Preparation method and application of stable phase inversion Pickering emulsion of hydrophobic H-SiO2-coated PNIPAM hybrid microgel

Abstract

The invention discloses a preparation method and application of a phase inversion Pickering emulsion with stable hydrophobic H-SiO2-coated PNIPAM hybridized microgel, and relates to the technical field of nano material preparation and biological culture. According to the preparation method, H-SiO2 and PNIPAM hybrid microgel particles with hydrophobic surfaces are prepared through a precipitation polymerization method and a sol-gel reaction, then the hybrid microgel is used as an emulsifier to stabilize a W / O type Pickering emulsion, obligate anaerobic bifidobacterium can be encapsulated in liquid drops of the emulsion, and efficient culture is achieved. The hybrid microgel prepared in the invention has strong hydrophobicity, and retains the thermosensitivity of the PNIPAM core, so the stable Pickering emulsion also has thermosensitivity, the emulsion can generate phase inversion only through temperature change, internal phase bacteria are released, and rapid separation is realized. Compared with a conventional anaerobic bacteria culture method, the method provided by the invention not only can realize efficient culture, but also can realize rapid separation of cultured bacteria, and has a good application prospect.

Images