Application of Chitinophaga eiseniae 4H in preparation of amino acid

Abstract

The invention discloses an application of Chitinophaga eiseniae 4H in the preparation of amino acid, and particularly discloses an application of the Chitinophaga eiseniae 4H in the preparation of amino acid. Chitinophaga eiseniae 4H (the preservation number is CCTCC NO: M 2022113) is inoculated into a culture medium of feather meal for culture, and after the feather is degraded and fermentation is performed for 24 hours, the total amino acid content in degradation liquid is increased by 8.14 times, the total essential amino acid content is increased by 7.0 times, and the contents of high-value amino acid tyrosine (Tyr), arginine (Arg) and cystine (Cys) are increased by 19.23, 7.10 and 1.8 times respectively, so that the recycling of waste resources is realized. The Chitinophaga eiseniae 4H is used for fermenting the feather meal, the fermentation time is short, the total amino acid, total essential amino acid and high-value amino acid after fermentation are high in increase multiple and high in concentration, and the fermentation liquor rich in amino acid has great potential in the aspects of medicine, food additives, amino acid production and cosmetic application.

Description

Technical Field

[0001] The invention belongs to the field of microorganisms, and specifically relates to the use of Chitinophaga eiseniae 4H in preparing total essential amino acids (phenylalanine (Phe), valine (Val), leucine (Leu), threonine (Thr), lysine (Lys), isoleucine (Ile) and methionine (Met)) and important high-value amino acids (tyrosine (Tyr), arginine (Arg) and cystine (Cys)). Background Art

[0002] With the intensive development of the breeding industry, my country produces millions of tons of waste feathers every year. The crude protein content in waste feathers is over 80%, and it contains 18 kinds of amino acids such as lysine, methionine, and threonine, as well as macro and trace elements and some unknown growth factors. There are a large number of disulfide bonds in the feather structure, and the cross-linking of disulfide bonds causes the feather structure to fold, making it difficult to degrade. In recent years, more and more feather-degrading microorganisms have been discovered. The microbial method is the most promising method for green manufacturing. Converting feathers into high-value-added amino acids can not only turn waste into treasure and realize the resource utilization of waste feathers, but also avoid their adverse effects on the environment. Summary of the invention

[0003] The invention provides application of Chitinophaga eiseniae 4H in preparing amino acids by fermenting feather powder. The Chitinophaga eiseniae 4H has a preservation number of CCTCC No: M 2022113.

[0004] Preferably, the amino acids are phenylalanine, valine, leucine, threonine, lysine, isoleucine and methionine.

[0005] Preferably, the amino acids are tyrosine, arginine and cystine.

[0006] Preferably, the feather meal is poultry duck feather meal.

[0007] Preferably, the feather meal is puffed duck feather meal.

[0008] The second object of the present invention is to provide a method for preparing amino acids using Chitinophaga eiseniae 4H, characterized in that amino acids are prepared by fermenting Chitinophaga eiseniae 4H in a culture medium containing feather powder, and the Chitinophaga eiseniae 4H has a preservation number of CCTCC No: M 2022113.

[0009] Preferably, the culture medium components and their contents are as follows: Feather meal 20 g·L -1 ,K2HPO41.4 g·L -1 , KH2PO40.7g·L -1 , NaCl 0.5 g·L -1 , MgSO40.1g·L -1 , the solvent is water.

[0010] The prepared amino acids are used in feed additives, amino acid production and cosmetics.

[0011] The invention uses Chitinophaga eiseniae 4H (with a deposit number of CCTCC NO: M 2022113) to be inoculated into a culture medium of feather powder for culture. After fermentation for 24 hours, the total amino acid content increases by 8.14 times, the total essential amino acid content increases by 7.0 times, and the high-value amino acids tyrosine (Tyr), arginine (Arg) and cystine (Cys) increase by 19.23, 7.10 and 1.8 times, respectively. The invention has great potential in feed additives, amino acid production and cosmetic applications.

[0012] Collection Instructions

[0013] The Chitinophaga eiseniae 4H of the present invention was deposited in the China Center for Type Culture Collection (CCTCC) on February 14, 2022, with an address of Wuhan University, Wuhan, China, zip code: 430072, and a deposit number of CCTCC NO: M2022113. The strain information is disclosed in the patent No. 202210508401.2, the invention name of which is the application of Chitinophaga eiseniae in degrading feather powder to produce biosurfactants. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 Types and concentrations (mg / L) of amino acids in the fermentation broth of Chitinophaga eiseniae 4H degrading feather meal at 0h and 24h. DETAILED DESCRIPTION

[0015] The following examples are provided to further illustrate the present invention, rather than to limit the present invention.

[0016] Embodiment 1:

[0017] 1. Feather degradation process

[0018] Chitinophaga eiseniae 4H was inoculated into a culture medium containing feather powder, and cultured at 30°C and 220 rpm to obtain a fermentation liquid. The composition and content of the culture medium were as follows: puffed duck feather powder 20 g·L -1 ,K2HPO41.4 g·L -1 , KH2PO40.7g·L -1 , NaCl 0.5 g·L -1 , MgSO40.1g·L -1 , pH 7.0, and the preparation method is to mix the ingredients evenly and sterilize them for later use.

[0019] Subsequently, the fermentation broth of 0h and 24h of feather meal degradation by Chitinophaga eiseniae 4H was taken to determine the amino acid content using an amino acid analyzer (Biochrom 30+, Biochrom / Biochrom, UK).

[0020] 2. Results

[0021] The amino acid composition of fermentation broth is of great significance for downstream applications, and the biological and functional activities of protein hydrolysates depend on the type and composition of amino acids in the protein sequence. Figure 1As shown, the fermentation liquid of Chitinophaga eiseniae 4H degrading feather meal at 0h and 24h had total amino acid contents of 200mg / L and 1628mg / L, respectively, and total essential amino acids (phenylalanine (Phe), valine (Val), leucine (Leu), threonine (Thr), lysine (Lys), isoleucine (Ile) and methionine (Met)) contents of 93mg / L and 651mg / L, respectively. After Chitinophaga eiseniae 4H fermented feather meal for 24h, the total amino acid content increased by 8.14 times, and the total essential amino acid content increased by 7.0 times. After 24h of Chitinophaga eiseniae 4H fermentation of feather meal, the concentrations of essential amino acids phenylalanine (Phe), valine (Val), leucine (Leu), threonine (Thr), lysine (Lys), isoleucine (Ile) and methionine (Met) increased by 25-, 18-, 13.10-, 8.5-, 6.50-, 2.9- and 1.0-fold respectively compared with 0h. In addition, tyrosine (Tyr), arginine (Arg) and cystine (Cys) are other important high-value amino acids with huge market demand. After 24h of fermentation, the concentrations of tyrosine (Tyr), arginine (Arg) and cystine (Cys) can reach 250mg / L, 71mg / L and 36mg / L, respectively, which are 19.23-, 7.10-, and 1.8-fold higher than 0h, respectively. Therefore, the fermentation broth of Chitinophaga eiseniae 4H fermented feather meal after 24h has great potential in feed additives, amino acid production and cosmetic applications.

Claims

1. Application of Chitinophaga eiseniae 4H in the preparation of amino acids by fermenting feather powder, wherein the Chitinophaga eiseniae 4H has a deposit number of CCTCC No: M 2022113.

2. The use according to claim 1, characterized in that: The amino acids are phenylalanine, valine, leucine, threonine, lysine, isoleucine and methionine.

3. The use according to claim 1, characterized in that: The amino acids are tyrosine, arginine and cystine.

4. The use according to claim 1, characterized in that: The feather meal is poultry duck feather meal.

5. The use according to claim 4, characterized in that: The feather powder is puffed duck feather powder.

6. A method for preparing amino acids using Chitinophaga eiseniae 4H, characterized in that: The amino acid is prepared by fermenting Chitinophaga eiseniae 4H in a culture medium containing feather powder. The Chitinophaga eiseniae 4H has a preservation number of CCTCC No: M 2022113.

7. The method according to claim 6, characterized in that The culture medium components and their contents are as follows: Feather powder 20 g·L -1 ,K2HPO41.4 g·L -1 ,KH2PO40.7 g·L -1 , NaCl 0.5 g·L -1 , MgSO40.1g·L -1 , the solvent is water.

8. The method according to claim 6, characterized in that The feather meal is poultry duck feather meal.

9. The method according to claim 8, characterized in that The feather powder is puffed duck feather powder.

10. The method according to claim 6, characterized in that The amino acids are phenylalanine, valine, leucine, threonine, lysine, isoleucine and methionine.

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