Construction method and application of electrochemical biosensor based on dual-conductive antifouling hydrogel

CN119355065AActive Publication Date: 2025-01-24QINGDAO UNIV OF SCI & TECH
3 Cites 1 Cited by

Patent Information

Application Number
CN202411255771.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-09-09
Publication Date
2025-01-24
Estimated Expiration
2044-09-09

AI Technical Summary

Technical Problem

Existing electrochemical sensors in complex biological environments have reduced detection sensitivity and accuracy due to the adsorption of nonspecific substances, especially in carcinoembryonic antigen (CEA) detection.

Method used

The ratio -type electrified student material sensor based on dual conductivity sewage gels is adopted. Through the use of MXENE, KH570, and PEDOT: PSS on the electrode interface, it enhances the electrical and anti -dirt resistance, and combines the interface prevention and ratio detection technology.

Benefits of technology

Significantly improves the detection sensitivity and accuracy of the electrochemical biosensor, achieving a low detection limit (0.41pg/mL) and a wide detection range (1pg/mL~1μg/mL) for CEA, and has excellent electrochemical stability and specificity.

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Abstract

The invention discloses a construction method and application of an electrochemical biosensor based on dual-conductive anti-fouling hydrogel, and belongs to the technical fields of biochemical analysis, functional nano materials and life science. In the invention, the antifouling conductive hydrogel is designed and synthesized by taking MXene as a conductive skeleton. Wherein the inherent surface hydrophilicity of the hydrogel endows the hydrogel with good anti-pollution capability. Meanwhile, due to the introduction of the gamma-methacryloxypropyltrimethoxysilane and the poly (3, 4-ethylenedioxythiophene)-polystyrolsulfon acid, the stability and the conductivity of the hydrogel are improved. Besides, the MXene nanosheet can load a large amount of [Ru (NH3) 6] < 3 + >, and effective integration of antifouling and internal standard is realized, so that the detection precision of the biosensor is improved. On the basis, the constructed electrochemical antifouling biosensor can realize sensitive detection on the carcino-embryonic antigen in a wide linear range, and the detection limit is as low as 0.41 pg / mL.
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Citation Information

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Cited By

  • Construction method and application of biosensor with double functions of autocatalytic enhancement and interface pollution resistance

    CN120629299A