A planting method and a harvesting method of evodia rutaecarpa

Abstract

This invention relates to the field of traditional Chinese medicinal herb cultivation technology, specifically to a method for cultivating and harvesting *Trifolium repens*. The method for cultivating *Trifolium repens* provided by this invention includes the following steps: applying a growth solution to the cut surface of the first harvest of *Trifolium repens*. The growth solution comprises a nutrient supplement and a cytokinin, wherein the concentration of the nutrient supplement is 1-2 mL / L and the concentration of the cytokinin is 3-4 mL / L. This invention, by applying a growth solution composed of a nutrient supplement and cytokinin to the cut surface of *Trifolium repens*, can improve tillering and increase the yield of the medicinal herb.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine cultivation technology, specifically to a cultivation method and harvesting method for the herb *Trifolium repens*. Background Technology

[0002] The medicinal material of *Trifolium repens* is *Trifolium repens*, a plant belonging to the Rutaceae family. Evodia lepta To ensure the supply of the dried stems and leafy young branches of *Spreng.* Merr., large-scale artificial cultivation and harvesting are mostly adopted to guarantee the market supply of *Spreng.* Merr.*.

[0003] Currently, traditional planting methods are generally used, and the entire plant is cut down during the harvest season. The herbs are then dried by sun-drying or oven-drying to obtain the three-forked bitter herb. However, traditional planting and harvesting methods are not only costly and inefficient, but also make it difficult to guarantee the uniformity of the herb's quality. Most importantly, the yield of the three-forked bitter herb is low. Summary of the Invention

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defect of low yield of Trifolium repens in the prior art, thereby providing a planting method and a harvesting method for Trifolium repens.

[0005] Therefore, the present invention provides a method for planting *Trifolium repens*, comprising the following steps: applying a growth solution to the cut surface of the first harvest of *Trifolium repens*, wherein the growth solution includes a nutrient supplement and a cytokinin, wherein the concentration of the nutrient supplement in the growth solution is 1-2 mL / L and the concentration of the cytokinin is 3-4 mL / L.

[0006] In some embodiments, the distance between the break of the first three-pronged pruning and the ground is 3-20cm, preferably 5-15cm.

[0007] In some embodiments, the nutrient supplement is a micronutrient fertilizer, wherein the total micronutrient content in the micronutrient fertilizer is greater than or equal to 100 g / L. Preferably, the trace elements in the trace element fertilizer include at least one of copper, iron, manganese, zinc, boron, and molybdenum.

[0008] More preferably, the micronutrient fertilizer conforms to the implementation standard NY 1428-2010.

[0009] In some embodiments, the cytokinin comprises benzylaminopurine, and the content of benzylaminopurine in the cytokinin is 3-8 wt%.

[0010] In some embodiments, the growth solution is prepared by dissolving the nutrient supplement and cytokinin separately in a solvent, and then mixing the dissolved nutrient supplement and the dissolved cytokinin to obtain the growth solution.

[0011] Preferably, water is used as the solvent for dissolving nutrient supplements or cytokinins.

[0012] In some embodiments, the harvesting period for Trifolium repens is from October to December, the first crop of Trifolium repens is planted for more than 3 years, and after the first crop is harvested, the second crop is planted for at least 2 years.

[0013] On the other hand, the present invention provides a method for harvesting the herb *Trifolium repens*, comprising the following steps: slicing and drying the harvested fresh *Trifolium repens* herb, wherein the drying is carried out by alternating drying and dehumidification.

[0014] Preferably, the fresh *Trifolium repens* medicinal material is obtained by the above-mentioned cultivation method of *Trifolium repens* medicinal material.

[0015] Preferably, the thickness of the sliced ​​fresh Trichosanthes kirilowii herb is 2-5 cm.

[0016] In some embodiments, before drying the sliced ​​fresh Trichosanthes kirilowii herb, the step of stacking the sliced ​​fresh Trichosanthes kirilowii herb to a height of 80-100cm is also included.

[0017] In some embodiments, the drying of sliced ​​fresh Trichosanthes kirilowii herb also includes a cold-drying step.

[0018] In some embodiments, the fresh *Trifolium repens* herb is stored for 5 days or less.

[0019] In some embodiments, the drying temperature is 55-60°C and the relative humidity is 10-40%.

[0020] In some embodiments, the drying time is 8-16 hours and the dehumidification time is 3-9 hours.

[0021] In some embodiments, the cold drying temperature is 12-18°C, the relative humidity is 8-15%, and the cold drying time is 0.5-1.5 hours.

[0022] Preferably, the drying steps are as follows: drying at a temperature of 55-65℃ and a relative humidity of 25-35% for 0.5-1.5 hours followed by dehumidification for 0.5-1.5 hours; drying at a temperature of 55-65℃ and a relative humidity of 15-25% for 0.5-1.5 hours followed by dehumidification for 0.5-1.5 hours; drying at a temperature of 50-60℃ and a relative humidity of 10-20% for 0.5-1.5 hours followed by dehumidification for 0.5-1.5 hours; drying at a temperature of 55-65℃ and a relative humidity of 10-20% for 3-5 hours followed by dehumidification for 0.5-1.5 hours; drying at a temperature of 55-65℃ and a relative humidity of 8-15% for 3-5 hours followed by dehumidification for 0.5-1.5 hours; and cold drying at a temperature of 10-20℃ and a relative humidity of 8-15% for 0.5-1.5 hours.

[0023] The technical solution of this invention has the following advantages: 1. This invention provides a method for cultivating *Trifolium repens*, comprising the following steps: applying a growth solution to the cut surface of the first harvest of *Trifolium repens*, wherein the growth solution includes a nutrient supplement and a cytokinin, wherein the concentration of the nutrient supplement is 1-2 mL / L and the concentration of the cytokinin is 3-4 mL / L. This invention, by applying a growth solution composed of a nutrient supplement and cytokinin to the cut surface of *Trifolium repens*, can improve tillering and increase the yield of the medicinal herb.

[0024] 2. This invention provides a method for planting *Trifolium repens*, wherein the distance between the cut surface of the first crop of *Trifolium repens* and the ground is 3-20 cm, preferably 5-15 cm. This invention limits the distance between the cut surface and the ground to prevent the cut surface from being too close to the ground, thus avoiding disease invasion, and also to prevent the cut surface from being too high, causing the tillering stem to approach the lenticels of the *Trifolium repens*, resulting in lodging. This, in turn, increases the yield of *Trifolium repens*.

[0025] 3. This invention provides a method for cultivating *Trifolium repens*, wherein the growth solution is prepared by dissolving a nutrient supplement and a cytokinin separately in water, and then mixing the dissolved nutrient supplement and the dissolved cytokinin to obtain the growth solution. This invention first completely dissolves the nutrient supplement before dissolving the cytokinin, avoiding the incompatibility of adding these two agents simultaneously, which would prevent the simultaneous promotion of tillering in *Trifolium repens* and shorten the planting time.

[0026] 4. This invention provides a method for harvesting *Trifolium repens*, comprising the following steps: slicing the harvested fresh *Trifolium repens* and drying it, wherein the drying is carried out using alternating drying and dehumidification methods. The drying method provided by this invention better preserves the effective components (heat-sensitive components) in the medicinal material, effectively removing moisture while maintaining the color of the material, reducing damage to the cell structure of the medicinal material, thereby maintaining the quality of the medicinal material. The method of drying fresh *Trifolium repens* using alternating drying and dehumidification not only improves the drying efficiency of *Trifolium repens*, but also increases the content of extractives from *Trifolium repens*, while effectively controlling the moisture content of the *Trifolium repens*, ensuring the uniformity of the medicinal material's quality.

[0027] 5. This invention provides a method for harvesting *Trifolium repens*, which includes a step of piling up the sliced ​​fresh *Trifolium repens* before drying, with the pile height being 80-100 cm. If the pile height is higher than 100 cm, the drying time is longer than 15 hours, resulting in higher energy consumption; if the pile height is lower than 80 cm, the drying time does not change much, but the total amount of processed herbs is reduced, and the energy utilization efficiency is lower. Attached Figure Description

[0028] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0029] Figure 1 This is a flowchart of a cultivation method for the herb *Trifolium repens* provided in Embodiment 1 of the present invention. Detailed Implementation The following embodiments are provided to better understand the present invention and are not limited to the preferred embodiments described. They do not constitute a limitation on the content and scope of protection of the present invention. Any product that is the same as or similar to the present invention, derived by any person under the guidance of the present invention or by combining the features of the present invention with other prior art, falls within the protection scope of the present invention.

[0030] For experiments not specifically described in the examples, the procedures or conditions should be followed according to the conventional experimental procedures described in the literature in this field. Reagents or instruments whose manufacturers are not specified are all commercially available conventional reagent products.

[0031] In the embodiments and comparative examples of this invention, the nutrient element supplement is a trace element water-soluble fertilizer (germination promoter) produced by Henan Qingnonghe Biotechnology Co., Ltd. Cytokinin was purchased from Henan Saier Agricultural Technology Co., Ltd., and the active ingredient, benzylaminopurine, had a mass fraction of 5%.

[0032] Example 1 Please see Figure 1 As shown in the figure, this embodiment provides a method for cultivating the herb *Trifolium repens*, with specific steps and parameters as follows: (1) Planting of Trifolium repens in March of the first year. Before planting, apply 0.5-1.0 kg of commercial organic fertilizer as base fertilizer in the planting hole. Weeding and loosening of soil and application of compound fertilizer are carried out in June and November of each year after planting. The amount of fertilizer is 100-150 g / plant. If no serious pests or diseases are found in the field, pesticides are generally not needed for control.

[0033] (2) The nutrient element supplement and the cytokinin were dissolved in water respectively. The dissolved nutrient element supplement and the dissolved cytokinin were mixed to obtain the growth solution. The concentration of the nutrient element supplement (produced by Henan Qingnonghe Biotechnology Co., Ltd. (trace element water-soluble fertilizer)) in the growth solution was 1 mL / L, and the concentration of the cytokinin (produced by Henan Saier Agricultural Technology Co., Ltd. (active ingredient content: benzylaminopurine 5%)) was 3 mL / L.

[0034] (3) In December of the third year of planting, cut the three-forked bitter herbs flat at a height of 15cm from the ground and apply a layer of growth solution to the cut surface. In the later period, weeding and loosening of soil and application of compound fertilizer are carried out in June and November of each year. The amount of fertilizer applied is 120g / plant. No serious pests and diseases are found in the field, and pesticides are generally not used for prevention and control.

[0035] (4) Harvest the three-forked bitter herb in December of the third year after applying the growth solution. The harvesting method is still to cut the three-forked bitter herb flat at a height of 15cm from the ground and apply a layer of growth solution to the cut surface.

[0036] Example 2 This embodiment provides a method for cultivating the herb *Trifolium repens*, with the specific steps and parameters as follows: (1) Planting of Trifolium repens in April of the first year. Before planting, apply 0.5-1.0 kg of commercial organic fertilizer as base fertilizer in the planting hole. Weeding and loosening of soil and application of compound fertilizer are carried out in June and November of each year after planting. The amount of fertilizer is 100-150 g / plant. If no serious pests or diseases are found in the field, pesticides are generally not used for control.

[0037] (2) The nutrient element supplement and the cytokinin were dissolved in water respectively. The dissolved nutrient element supplement and the dissolved cytokinin were mixed to obtain the growth solution. The concentration of the nutrient element supplement (produced by Henan Qingnonghe Biotechnology Co., Ltd. (trace element water-soluble fertilizer)) in the growth solution was 2 mL / L, and the concentration of the cytokinin (produced by Henan Saier Agricultural Technology Co., Ltd. (active ingredient content: benzylaminopurine 5%)) was 3 mL / L.

[0038] (3) In November of the third year of planting, cut the three-forked bitter herbs flat at a height of 10cm from the ground and apply a layer of growth solution to the cut surface. In the later period, weeding and loosening of soil and top dressing of compound fertilizer are carried out in June and November of each year. The amount of fertilizer applied is 100~150g / plant. If no serious pests or diseases are found in the field, pesticides are generally not used for prevention and control.

[0039] (4) Harvest the three-forked bitter herb in November of the third year after applying the growth solution. The harvesting method is still to cut the three-forked bitter herb flat at a height of 10cm from the ground and apply a layer of growth solution to the cut surface.

[0040] Example 3 This embodiment provides a method for cultivating the herb *Trifolium repens*, with the specific steps and parameters as follows: (1) Planting of Trifolium repens in March of the first year. Before planting, apply 0.5-1.0 kg of commercial organic fertilizer as base fertilizer in the planting hole. Weeding and loosening of soil and application of compound fertilizer are carried out in June and November of each year after planting. The amount of fertilizer is 100-150 g / plant. If no serious pests or diseases are found in the field, pesticides are generally not needed for control.

[0041] (2) Mix the nutrient element supplement and cytokinin to obtain the growth solution, wherein the concentration of the nutrient element supplement (produced by Henan Qingnonghe Biotechnology Co., Ltd. (trace element water-soluble fertilizer)) in the growth solution is 2 mL / L, and the concentration of cytokinin (produced by Henan Saier Agricultural Technology Co., Ltd. (active ingredient content: benzylaminopurine 5%)) is 4 mL / L.

[0042] (3) In December of the third year of planting, cut the three-forked bitter herbs flat at a height of 5cm from the ground and apply a layer of growth solution to the cut surface. In the later period, weeding and loosening of soil and application of compound fertilizer are carried out in June and November of each year. The amount of fertilizer is 100~150g / plant. If no serious pests or diseases are found in the field, pesticides are generally not used for prevention and control.

[0043] (4) Harvest the three-forked bitter herb in December of the third year after applying the growth solution. The harvesting method is still to cut the three-forked bitter herb on a flat surface 5cm above the ground and apply a layer of growth solution to the cut surface.

[0044] Example 4 This embodiment provides a method for planting the herb *Trifolium repens*. The specific steps and parameters are the same as in embodiment 1. The difference is that in step (2), the location where *Trifolium repens* is cut is 3 cm from the ground.

[0045] Example 5 This embodiment provides a method for planting the herb *Trifolium repens*. The specific steps and parameters are the same as in embodiment 1. The difference is that in step (2), the location where *Trifolium repens* is cut is 20cm from the ground.

[0046] Comparative Example 1 This comparative example provides a method for cultivating Trifoliate bitter herb. The specific steps and parameters are the same as in Example 1. The difference is that after cutting in step (3), growth solution was not applied to the cut surface.

[0047] That is (3) in December of the third year of planting, cut the three-forked bitter herbs flat at a height of 15cm from the ground. In the later period, weeding and loosening of the soil and application of compound fertilizer are carried out in June and November of each year. The amount of fertilizer applied is 120g / plant. No serious diseases or pests are found in the field, and pesticides are generally not used for prevention and control.

[0048] Comparative Example 2 This comparative example provides a method for cultivating Trifoliate bitter herb. The specific steps and parameters are the same as in Example 1. The difference is that the growth solution in step (2) does not contain nutrient supplements.

[0049] That is, (1) use cytokinin with a concentration of 3 mL / L as the growth medium.

[0050] Comparative Example 3 This comparative example provides a method for cultivating Trichosanthes kirilowii, with the specific steps and parameters being the same as in Example 1. The difference is that the growth solution in step (2) does not contain cytokinins.

[0051] That is, (1) a nutrient element supplement with a concentration of 1 mL / L is used as the growth solution.

[0052] Comparative Example 4 This comparative example provides a method for cultivating *Trifolium repens*, with the specific steps and parameters being the same as in Example 1, except that the concentration of the nutrient supplement in the growth solution is 0.5 mL / L.

[0053] Comparative Example 5 This comparative example provides a method for cultivating Trifoliate bitter herb. The specific steps and parameters are the same as in Example 1. The only difference is that the concentration of the nutrient supplement in the growth solution in step (2) is 2.5 mL / L.

[0054] Comparative Example 6 This comparative example provides a method for cultivating Trichoderma trifoliata, with the same specific steps and parameters as in Example 1. The only difference is that the concentration of cytokinin in the growth solution in step (2) is 2.5 mL / L.

[0055] Comparative Example 7 This comparative example provides a method for cultivating Trifoliate bitter herb. The specific steps and parameters are the same as in Example 1. The only difference is that the concentration of the nutrient supplement in the growth solution in step (2) is 4.5 mL / L.

[0056] Application Example 1 This application example provides a method for harvesting the herb *Trifolium repens*, with the specific steps and parameters as follows: The fresh *Trifolium repens* harvested in step (4) of Examples 1 and Comparative Examples 1-7 was sliced ​​using a slicer. The slice thickness was 5 mm. The fresh *Trifolium repens* harvested was stored for 5 days. The sliced ​​*Trifolium repens* was stacked in the intelligent air-source heat pump integrated drying equipment room with a stack height of 100 cm. The sliced ​​*Trifolium repens* was dried according to the following drying procedure to obtain *Trifolium repens*.

[0057] The drying process consists of the first stage, which uses a drying and dehumidification mode, at 60℃ and 30% RH, drying for 1 hour and dehumidification for 1 hour. The second stage uses a drying and dehumidification mode, drying for 1 hour and dehumidification for 1 hour at 60℃ and 20% RH. The third stage uses a drying and dehumidification mode, drying for 1 hour and dehumidification for 1 hour at 55℃ and 15RH% humidity. In the fourth stage, a drying and dehumidification mode is adopted, with drying at 60℃ and 15% RH for 4 hours and dehumidification for 1 hour. In the fifth stage, a drying and dehumidification mode is adopted, with drying at 60℃ and 10% RH for 4 hours and dehumidification for 1 hour. In the sixth stage, a cold drying mode is adopted, with a temperature of 15℃, a humidity of 10% and a drying time of 1 hour.

[0058] The dried bitter herb is conveyed by a conveyor belt to a separate packaging area for cooling, and then packaged in food-grade yellow double-layered woven bags. Each bag is pre-quantified, labeled, and then transferred to the warehouse for storage. The pre-quantified packaging of bitter herb is 40kg / bag.

[0059] Application Example 2 This application example provides a method for harvesting the herb *Trifolium repens*, with the specific steps and parameters as follows: The fresh *Trifolium repens* harvested in step (4) of Example 2 was sliced ​​using a slicer. The slice thickness was 4 mm. The fresh *Trifolium repens* harvested was stored for 4 days. The sliced ​​*Trifolium repens* was stacked in the intelligent air-source heat pump integrated drying equipment room with a stack height of 90 cm. The sliced ​​*Trifolium repens* was dried according to the following drying procedure to obtain *Trifolium repens*.

[0060] The drying process consists of the first stage, which uses a drying and dehumidification mode, at 60℃ and 30% RH, drying for 1 hour and dehumidification for 1 hour. The second stage uses a drying and dehumidification mode, drying for 1 hour and dehumidification for 1 hour at 60℃ and 20% RH. The third stage uses a drying and dehumidification mode, drying for 1 hour and dehumidification for 1 hour at 55℃ and 15RH% humidity. In the fourth stage, a drying and dehumidification mode is adopted, with drying at 60℃ and 15% RH for 4 hours and dehumidification for 1 hour. In the fifth stage, a drying and dehumidification mode is adopted, with drying at 60℃ and 10% RH for 4 hours and dehumidification for 1 hour. In the sixth stage, a cold drying mode is adopted, with humidity at 15℃ and 10% RH for 1 hour.

[0061] The dried bitter herb is conveyed by a conveyor belt to a separate packaging area for cooling, and then packaged in food-grade yellow double-layered woven bags. Each bag is pre-quantified, labeled, and then transferred to the warehouse for storage. The pre-quantified packaging of bitter herb is 40kg / bag.

[0062] Application Example 3 This application example provides a method for harvesting the herb *Trifolium repens*, with the specific steps and parameters as follows: The fresh *Trifolium repens* harvested in step (4) of Example 3 was sliced ​​using a slicer. The slices were 2 mm thick. The fresh *Trifolium repens* harvested was stored for 3 days. The sliced ​​*Trifolium repens* was stacked in the intelligent air-source heat pump integrated drying equipment room with a stacking height of 80 cm. The sliced ​​*Trifolium repens* was dried according to the following drying procedure to obtain the *Trifolium repens*.

[0063] The drying process consists of the first stage, which uses a drying and dehumidification mode, at 60℃ and 30% RH, drying for 1 hour and dehumidification for 1 hour. The second stage uses a drying and dehumidification mode, drying for 1 hour and dehumidification for 1 hour at 60℃ and 20% RH. The third stage uses a drying and dehumidification mode, drying for 1 hour and dehumidification for 1 hour at 55℃ and 15RH% humidity. In the fourth stage, a drying and dehumidification mode is adopted, with drying at 60℃ and 15% RH for 4 hours and dehumidification for 1 hour. In the fifth stage, a drying and dehumidification mode is adopted, with drying at 60℃ and 10% RH for 4 hours and dehumidification for 1 hour. In the sixth stage, a cold drying mode is adopted, with humidity at 15℃ and 10% RH for 1 hour.

[0064] The dried bitter herb is conveyed by a conveyor belt to a separate packaging area for cooling, and then packaged in food-grade yellow double-layered woven bags. Each bag is pre-quantified, labeled, and then transferred to the warehouse for storage. The pre-quantified packaging of bitter herb is 40kg / bag.

[0065] Application Example 4 This application example provides a method for harvesting the herb *Trifolium repens*. The specific steps and parameters are the same as in application example 1. The difference is that the fresh herb *Trifolium repens* is obtained by cutting it down in a plane at a distance of 15cm from the ground in step (3) of example 1 (the first harvest of *Trifolium repens*, i.e., the *Trifolium repens* cut down before applying growth solution).

[0066] Application Example 5 This application example provides a method for harvesting the herb *Trifolium repens*. The specific steps and parameters are the same as in Application Example 1. The difference is that a sunroom is used to dry the sliced ​​*Trifolium repens* for 60 hours.

[0067] Application Example 6 This application example provides a method for harvesting the herb *Trifolium repens*. The specific steps and parameters are the same as in Application Example 1. The difference is that the drying program is a constant temperature drying and dehumidification mode, drying at 40℃ for 1 hour and dehumidifying for 1 hour, for a total of 50 hours.

[0068] Application Example 7 This application example provides a method for harvesting the herb *Trifolium repens*. The specific steps and parameters are the same as in Application Example 1. The difference is that the drying program is a constant temperature drying and dehumidification mode, drying at 60℃ for 1 hour and dehumidifying for 1 hour, for a total of 35 hours.

[0069] Application Example 8 This application example provides a method for harvesting the herb *Trifolium repens*. The specific steps and parameters are the same as in Application Example 1. The difference is that the drying program is a constant temperature cold drying mode, which involves cold drying at 15°C for 80 hours.

[0070] Application Example 9 This application example provides a method for harvesting the herb *Trifolium repens*, with the specific steps and parameters as follows: The fresh *Trifolium repens* harvested in step (4) of Example 1 was sliced ​​using a slicer. The slice thickness was 5 mm. The fresh *Trifolium repens* harvested was stored for 5 days. The sliced ​​*Trifolium repens* was stacked in the intelligent air-source heat pump integrated drying equipment room with a stack height of 100 cm. The sliced ​​*Trifolium repens* was dried according to the following drying procedure to obtain *Trifolium repens*.

[0071] The drying process consists of the first stage, which uses a drying and dehumidification mode, at 55℃ and 35% RH, for 1.5 hours of drying and 0.5 hours of dehumidification. In the second stage, a drying and dehumidification mode is adopted, with drying at 65℃ and 15% RH for 1.5 hours and dehumidification for 0.5 hours. The third stage uses a drying and dehumidification mode, drying for 0.5 hours and dehumidification for 1.5 hours at 50℃ and 20% RH. In the fourth stage, a drying and dehumidification mode is adopted, with drying at 65℃ and 10% RH for 3 hours and dehumidification for 1.5 hours. In the fifth stage, a drying and dehumidification mode is adopted, with drying at 55℃ and 15% RH for 5 hours and dehumidification for 0.5 hours. The sixth stage uses a cold drying mode, at 20℃ and 8% RH, for 1.5 hours.

[0072] The dried bitter herb is conveyed by a conveyor belt to a separate packaging area for cooling, and then packaged in food-grade yellow double-layered woven bags. Each bag is pre-quantified, labeled, and then transferred to the warehouse for storage. The pre-quantified packaging of bitter herb is 40kg / bag.

[0073] Application Example 10 This application example provides a method for harvesting the herb *Trifolium repens*, with the specific steps and parameters as follows: The fresh *Trifolium repens* harvested in step (3) of Examples 1 and Comparative Examples 1-7 was sliced ​​using a slicer. The slice thickness was 5 mm. The fresh *Trifolium repens* harvested was stored for 5 days. The sliced ​​*Trifolium repens* was stacked in the intelligent air-source heat pump integrated drying equipment room with a stacking height of 100 cm. The sliced ​​*Trifolium repens* was dried according to the following drying procedure to obtain *Trifolium repens*.

[0074] The drying process consists of the first stage, which uses a drying and dehumidification mode, at 65℃ and 25% RH, for 0.5 hours of drying and 1.5 hours of dehumidification. In the second stage, a drying and dehumidification mode is adopted, with drying at 55℃ and 25% RH for 0.5 hours and dehumidification for 1.5 hours. The third stage uses a drying and dehumidification mode, drying for 1.5 hours and dehumidification for 0.5 hours at 60℃ and 10% RH. In the fourth stage, a drying and dehumidification mode is adopted, with drying at 55℃ and 20% RH for 5 hours and dehumidification for 0.5 hours. In the fifth stage, a drying and dehumidification mode is adopted, with drying at 65℃ and 8% RH for 3 hours and dehumidification for 1.5 hours. The sixth stage uses a cold drying mode, at 10℃ and 15% RH, for 0.5 hours.

[0075] The dried bitter herb is conveyed by a conveyor belt to a separate packaging area for cooling, and then packaged in food-grade yellow double-layered woven bags. Each bag is pre-quantified, labeled, and then transferred to the warehouse for storage. The pre-quantified packaging of bitter herb is 40kg / bag.

[0076] Experimental Example 1 For the *Trifolium repens* planted in Examples 1-5 and Comparative Examples 1-7, the number of shoots sprouting from the base of the main stem at the cut site was counted in March of the year following the application of the growth solution. The yield of medicinal materials after harvesting in the third year after the application of the growth solution (the year of application of the growth solution is considered the first year, and the harvest time is considered the third year) and the mold rate after 5 days of temporary storage of the harvested medicinal materials (after the medicinal materials are temporarily stored, there is a layer of black mold on the surface, and the mold rate = number of moldy medicinal materials / total number of medicinal materials × 100) were tested. The results are shown in Table 1.

[0077] Table 1. Detection results of Trichosanthes kirilowii (a type of medicinal herb).

[0078] According to the data in Table 1, compared with Comparative Examples 1-3, the growth solution formed by the nutrient element supplement and cytokinin used in this invention has a synergistic effect in promoting the number of branches and new roots of Trifolium repens. Among them, Comparative Example 1 had fewer new branches due to the lack of growth solution, and the branches withered and died during the later growth process.

[0079] Compared to comparative examples 4-7, the present invention, by controlling the concentration of nutrient supplement in the growth solution to 1-2 mL / L and the concentration of cytokinin to 3-4 mL / L, significantly promotes the growth of branch number and new roots in *Trifolium repens*. Therefore, the growth solution formed by the nutrient supplement and cytokinin in this invention can effectively increase the number of branches and new roots in the second crop of *Trifolium repens*, and the yield of the second crop of medicinal materials.

[0080] Compared to Examples 4-5, when the main stem left after felling is less than 5cm from the ground, the newly sprouted branches are easily infected with diseases. When the main stem left after felling is more than 20cm from the ground, it will cause the branches to sprout at the lenticels at the base of the main stem, resulting in the new branches lodging and affecting the yield of the second crop of bitter herbs. This invention limits the felling distance to 5-20cm from the ground, reducing the infection and lodging problems of diseases during the cultivation of bitter herbs, thereby increasing the yield of the second crop of bitter herbs.

[0081] Meanwhile, the traditional planting method results in a three-year harvest cycle for Trifolium repens. However, the planting method provided by this invention uses the application of growth solution as the current year, allowing for the harvesting of Trifolium repens in the third year. Thus, the planting method provided by this invention can shorten the harvest cycle to 2 years.

[0082] Experimental Example 2 The moisture content, extract, ash content, and drying cost of the dried Trichosanthes kirilowii medicinal material were measured according to cases 1-8, and the results are shown in Table 2.

[0083] The method for determining the moisture content of *Trifolium repens* medicinal material is as follows: Take 5g of the sample. If the diameter or length of the sample exceeds 3mm, it should be quickly prepared into granules or fragments with a diameter or length not exceeding 3mm before weighing. Spread these granules or fragments evenly in a flat weighing bottle that has been dried to constant weight, with a thickness not exceeding 5mm. For loose samples, the thickness should not exceed 10mm. Weigh accurately. Open the bottle cap and dry at 102±2℃ for 5 hours. Close the cap, transfer the bottle to a desiccator, and cool for 30 minutes. Weigh accurately again. Dry at the same temperature for another hour, cool, and weigh until the difference between two consecutive weighings does not exceed 5mg. Calculate the moisture content (%) of the sample based on the weight loss: Moisture content (%) = Weight loss (g) / Total weight of sample (g) × 100.

[0084] Method for detecting extracts from medicinal materials - Hot extraction method: Accurately weigh approximately 2g of the test sample and place it in a 250mL Erlenmeyer flask. Accurately add 50mL of ethanol, seal tightly, weigh, and let stand for 1 hour. Then connect a reflux condenser, heat to boiling, and maintain a gentle boil for 1 hour. After cooling, remove the Erlenmeyer flask, seal tightly, weigh again, replenish the lost weight with ethanol, shake well, filter through a dry filter, accurately measure 25mL of the filtrate, place it in an evaporating dish that has been dried to constant weight, evaporate to dryness on a water bath, dry at 105℃ for 3 hours, cool in a desiccator for 30 minutes, and quickly weigh accurately. Unless otherwise specified, calculate the content (%) of alcohol-soluble extracts in the test sample based on the dried product: Extract content (%) = dried product weight (g) / test sample weight (g) × 100.

[0085] Method for determining the ash content of medicinal materials: For the total ash determination method, the sample must be pulverized to pass through a No. 2 sieve. After thorough mixing, take 2g of the sample (5g if acid-insoluble ash is to be determined), place it in a crucible heated to constant weight, and weigh it (accurate to 0.01g). Heat slowly, taking care to avoid combustion, until complete carbonization. Gradually increase the temperature to 500℃ to ensure complete ashing and constant weight. Calculate the total ash content (%) in the sample based on the residue weight. If the sample is difficult to ashing, cool the crucible, add 2mL of hot water or 10% ammonium nitrate solution to moisten the residue, then evaporate it to dryness on a water bath. Heat the residue again as before until the crucible contents are completely ashing. The total ash content (%) in the sample = residue weight (g) / sample weight (g) × 100.

[0086] Method for calculating the cost of drying medicinal materials (excluding outsourced testing fees): Drying cost = Dryer energy consumption cost × Drying time + Operator wages + Other expenses Table 2. Test results of dried Trichosanthes kirilowii root.

[0087] As shown in Table 3, compared with Application Examples 5-8, the staged drying method of the present invention for drying *Trifolium repens* significantly increases the extract content of *Trifolium repens*, reduces the ash content and moisture content of the herb, and simultaneously lowers processing costs. Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A method for planting Evodia lepta, characterized in that, Includes the following steps, Apply growth hormone to the cut surface of the first batch of three-pronged bitterling. The growth medium includes a nutrient supplement and a cytokinin, wherein the concentration of the nutrient supplement in the growth medium is 1-2 mL / L and the concentration of the cytokinin is 3-4 mL / L. The nutrient supplement is a micronutrient fertilizer, and the total micronutrient content in the micronutrient fertilizer is greater than or equal to 100g / L. The micronutrients in the micronutrient fertilizer include at least one of copper, iron, manganese, zinc, boron, and molybdenum. The cytokinin includes benzylaminopurine, and the content of benzylaminopurine in the cytokinin is 3-8 wt%. The distance between the cut surface of the first batch of three-pronged pruning and the ground is 5-15cm; The first crop of Trifoliate Bitter Orange was planted more than 3 years ago; The harvesting period for *Trifolium repens* is from October to December. After the first harvest, the second planting period is at least two years. The harvested fresh *Trifolium repens* herbs are sliced ​​and dried. The drying steps are as follows: Dry at 55-65℃ and 25-35% relative humidity for 0.5-1.5 hours, then remove moisture for 0.5-1.5 hours. Dry at 55-65℃ and 15-25% relative humidity for 0.5-1.5 hours, then remove moisture for 0.5-1.5 hours. Dry at 50-60℃ and 10-20% relative humidity for 0.5-1.5 hours, then remove moisture for 0.5-1.5 hours. After drying at a temperature of 55-65℃ and a relative humidity of 10-20% for 3-5 hours, remove moisture for 0.5-1.5 hours. After drying at 55-65℃ and 8-15% relative humidity for 3-5 hours, remove moisture for 0.5-1.5 hours. Cold dry for 0.5-1.5 hours at a temperature of 10-20℃ and a relative humidity of 8-15%. After the initial drying process, the product is then subjected to cold drying at a temperature of 12-18°C, a relative humidity of 8-15%, and a drying time of 0.5-1.5 hours.

2. The planting method of the Radix et Rhizoma Tripterygii according to claim 1, characterized in that, The growth solution is prepared by dissolving the nutrient supplement and cytokinin separately in a solvent, and then mixing the dissolved nutrient supplement and cytokinin to obtain the growth solution.

3. The method for cultivating the herb *Trifolium repens* according to claim 1, characterized in that, Water is used as the solvent to dissolve nutrient supplements or cytokinins.

4. The planting method of Radix et Rhizoma Tripterygii according to any one of claims 1-3, characterized in that, The thickness of the sliced ​​fresh Trichosanthes kirilowii herb is 2-5 cm.

5. The method of claim 1-3, wherein the method is characterized by, Before drying the sliced ​​fresh Trichosanthes kirilowii medicinal material, the process also includes stacking the sliced ​​fresh Trichosanthes kirilowii medicinal material to a height of 80-100cm.

6. The method for cultivating the herb *Trifolium repens* according to any one of claims 1-3, characterized in that, The fresh *Trifolium repens* medicinal material must be stored for no more than 5 days.

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