Primer combination for detecting rice MITE transposon insertion site and application thereof
CN119372369AActive Publication Date: 2025-01-28SANYA NATIONAL INSTITUTE OF SOUTHERN BREEDING CHINESE ACADEMY OF AGRICULTURAL SCIENCES +1
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Patent Information
- Application Number
- CN202411920873.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-25
- Publication Date
- 2025-01-28
- Estimated Expiration
- 2044-12-25
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Figure CN119372369A_ABST
Abstract
The invention relates to the field of molecular biology, in particular to a primer group for detecting rice MITE transposon insertion sites and application of the primer group. The primer group comprises 16 specific primers, and the sequences of the specific primers are as shown in SEQ ID NO: 11-26; about 2.90 thousand MITE loci can be enriched from each rice material by utilizing any single primer or a mixed combination of any several primers in the primer group through a method of establishing a library for PCR (Polymerase Chain Reaction) and then carrying out NGS second-generation deep sequencing, and the detection rate exceeds 80%; the invention provides a powerful tool for rice MITE site detection, and has important technical guidance significance in the fields of rice rapid molecular breeding, germplasm identification and the like.
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Citation Information
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