Primer group and application thereof

Abstract

The invention belongs to the technical field of sequencing, and particularly relates to a primer group and application thereof. The invention provides a primer group, the primer group can be amplified through a one-step method or a two-step method to obtain a target library containing a normal sequencing linker sequence, an intermediate product does not need to be purified during amplification through the two-step method, and the obtained amplification product has specificity and is not duplication; the primer group can be used for mNGS and / or tNGS, and when the primer group is used for mNGS, detection of a target gene can be improved; when being used for tNGS, the kit can improve the concentration of effective target fragments in a library to be captured, improve the efficiency of pathogenic microorganism probe capture and avoid false positive.