A method for cultivating *Striga asiatica* in pots

Abstract

This application relates to a method for potted cultivation of *Striga asiatica*, which involves pre-culturing mature *Striga asiatica* seeds successively with water and a hormone solution containing a specific concentration of strigolactone. The limited pre-culturing time effectively promotes seed germination in soil and enhances the activity of early growth stages, significantly shortening the germination time in soil and achieving rapid seedling emergence in soil-grown *Striga asiatica*. Furthermore, the selection of substrate and host plant based on this method optimizes the drought tolerance and disease and pest resistance of *Striga asiatica* seeds, improving their resistance to environmental stress and ultimately resulting in high-quality seedlings.

Description

Technical Field

[0001] This application relates to the field of plant cultivation technology, and in particular to a method for potted cultivation of *Striga asiatica*. Background Technology

[0002] *Striga asiatica*, belonging to the Scrophulariaceae family, is an annual semi-parasitic herb that typically parasitizes the roots of grasses. It grows in barren mountains, valleys, grasslands, and field edges, primarily distributed in Guangdong, Fujian, Jiangxi, Guangxi, Yunnan, and Guizhou provinces of my country. Also known as *Ganjicao*, *Aijiaozi*, *Dujiaogan*, and *Xiaomichong*, the dried whole herb is used medicinally. The medicinal material, *Striga asiatica*, has the effects of strengthening the spleen, eliminating food stagnation, clearing heat, and killing parasites. It is a traditional medicinal plant in southern my country, commonly used in Guangdong and Guangxi to treat infantile indigestion and liver fire with good results. Clinically, it is mainly used to treat infantile malnutrition, indigestion, jaundice hepatitis, and anorexia. Modern research shows it also has potential for antioxidant, antibacterial, and immunomodulatory effects. Due to its unique semi-parasitic characteristics, the seeds of *Striga asiatica* have a deep dormancy period, with a germination rate of 0% under natural conditions without a host. Under natural conditions, *Striga asiatica* seeds require a specific environment and stimulation from host secretions to germinate. Even after stimulating seed germination using existing technologies, *Striga asiatica* still needs to find a suitable host and establish a parasitic relationship. It must absorb nutrients from the host to maintain its growth and development, completing its life cycle from germination and seedling formation to flowering and fruiting. These characteristics result in weak competitiveness and slow natural regeneration within *Striga asiatica* communities. In recent years, market demand for *Striga asiatica* has increased, and excessive harvesting and environmental destruction have led to the near extinction of wild *Striga asiatica* resources. Therefore, artificial cultivation methods have emerged. The primary goal of artificial cultivation is to break the dormancy of *Striga asiatica* seeds to achieve a high germination rate.

[0003] However, existing methods for artificially cultivating *Striga asiatica* are mostly carried out under laboratory conditions. Although these methods can promote a high germination rate, they have stringent requirements for the laboratory culture environment, requiring the use of MS medium and strict aseptic treatment and management, which is very expensive and has no practical commercial value. On the other hand, the few existing methods for soil cultivation of *Striga asiatica* result in excessively long germination times, low seedling health rates, and low survival rates. Summary of the Invention

[0004] Therefore, it is necessary to provide a potted cultivation method for *Striga asiatica* to address the aforementioned technical problems. This method allows for the rapid germination of *Striga asiatica* seedlings in pots, enabling the production of high-quality *Striga asiatica* plants within a short period.

[0005] To achieve the above objectives, the embodiments of the present invention adopt the following technical solutions:

[0006] A method for cultivating *Striga asiatica* in pots, such as... Figure 1 As shown, it includes:

[0007] Step A: Select the substrate and host plant. Cultivate the host plant in the substrate until it grows to 10-15cm. Host plants include corn, crabgrass, and goosegrass. The substrate is a mixture of soil (clay) from the habitat of *Striga asiatica* and sand, or a mixture of nutrient soil and sand, or a mixture of nutrient soil, sand, and perlite.

[0008] Step B: Take mature *Striga asiatica* seeds and culture them in clean water for 3-8 days. After removing them, culture them in a hormone solution for 3-8 days. Mature seeds are those that are plump and have a brownish-black seed coat. The hormone solution should be 10... -8 ~10 -3 mol / L strigolactone solution;

[0009] Step C: Take out the seeds of *Striga asiatica* and wash them with sterile water. Inoculate the washed seeds of *Striga asiatica* around the roots of the host plant.

[0010] Step D: Perform routine watering management on the Strigolactone seeds.

[0011] Preferably, the matrix combination methods include:

[0012] The substrate is a 1:1 mixture of soil (clay) and sand from the habitat of *Striga asiatica*, or a 1:1 mixture of nutrient soil and sand, or a 1:1:1 mixture of nutrient soil, sand and perlite; the nutrient soil composition is as follows: 45% coconut coir, 25% peat moss, 5% corn cob residue, 5% xylose residue, 5% wood ash, 5% wheat bran, 3% Bacillus subtilis, 3% humic acid, and 4% perlite.

[0013] Preferably, the seeds of *Striga asiatica* are cultured in clean water for 3-8 days in a dark environment at a water temperature of 30-32 degrees Celsius; after being removed, the seeds are cultured in a hormone solution for 3-8 days in a dark environment at a hormone solution temperature of 32-35 degrees Celsius.

[0014] Preferably, before soaking the *Striga asiatica* seeds in the hormone solution, the seeds are first disinfected with a 3% sodium hypochlorite solution for 3 minutes.

[0015] Strigolactone is a sesquiterpene lactone compound extracted from the plant *Striga asiatica*. Hormone solutions containing strigolactone can regulate seed cell division, elongation, and other physiological functions. -8 ~10 -3The mol / L concentration is the optimized concentration obtained by our team after multiple experiments. It can effectively promote seed germination and subsequent seedling growth without causing adverse effects due to drug overdose. The solution temperature is set at 34℃, which is suitable for the chemical activity of strigolactone and provides a warm environment for seed germination, further activating the internal growth process of the seed and further promoting the germination and seedling growth of strigolactone seeds in the soil environment.

[0016] The above technical solution has the following advantages and beneficial effects:

[0017] By pre-culturing mature *Striga asiatica* seeds successively with water and a hormone solution containing strigolactone at a specific concentration, and limiting the pre-culturing time, the germination of *Striga asiatica* seeds in soil and the enhancement of their activity in the early growth stage can be effectively promoted. This significantly shortens the emergence time of *Striga asiatica* in soil environment, achieving rapid seedling emergence in soil culture. Furthermore, the selection of substrate and host plants based on this method optimizes the drought tolerance and disease and pest resistance of *Striga asiatica* seeds, improving their resistance to environmental stress and ultimately resulting in high-quality seedlings. Attached Figure Description

[0018] Figure 1 A flowchart illustrating the potted cultivation method for *Striga asiatica*.

[0019] Figure 2 This is a schematic diagram of the *Striga asiatica* potted plant after 19 days of planting in Example 1;

[0020] Figure 3 This is a schematic diagram of the *Striga asiatica* potted plant after 33 days of cultivation in Example 1;

[0021] Figure 4 This is a schematic diagram of the *Striga asiatica* potted plant after 40 days of planting in Example 1;

[0022] Figure 5 This is a schematic diagram of the *Striga asiatica* potted plant after 20 days of planting in Example 2;

[0023] Figure 6 This is a schematic diagram of the *Striga asiatica* potted plant after 30 days of planting in Example 2;

[0024] Figure 7 This is a schematic diagram of the *Striga asiatica* potted plant after 38 days of cultivation in Example 2;

[0025] Figure 8 This is a schematic diagram of the *Striga asiatica* potted plant after 16 days of planting in Example 3;

[0026] Figure 9 This is a schematic diagram of the *Striga asiatica* potted plant after 35 days of planting in Example 3;

[0027] Figure 10This is a schematic diagram of the *Striga asiatica* potted plant after 43 days of cultivation in Example 3; Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application.

[0029] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

[0030] Furthermore, the technical solutions of the various embodiments of the present invention can be combined with each other, but only if they are implemented by those skilled in the art. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such combination of technical solutions does not exist and is not within the scope of protection claimed by the present invention.

[0031] Example 1

[0032] A method for cultivating *Striga asiatica* in pots is provided, including the following steps:

[0033] Step A: Select a 1:1 mixture of soil (clay) and sand from the habitat of *Striga asiatica*, and select corn and crabgrass as host plants. Cultivate corn and crabgrass in the soil (clay) substrate from the habitat of *Striga asiatica* until they grow to 14cm.

[0034] Step B: Take mature *Striga asiatica* seeds and cultivate them in clean water for 5 days in a dark environment at a water temperature of 30 degrees Celsius. After removing them, place the seeds in a hormone solution for 5 days in a dark environment at a hormone solution temperature of 34 degrees Celsius. Mature seeds are plump and have a brownish-black seed coat. The hormone solution concentration is 5*10. -6 mol / L strigolactone solution;

[0035] Step C: Take out the seeds of *Striga asiatica* and wash them with sterile water. Inoculate the washed seeds of *Striga asiatica* around the roots of the host plant.

[0036] Step D: Perform routine watering management on the Strigolactone seeds.

[0037] It is understandable that the soil (clay) in the habitat of *Striga asiatica* has fine particles (usually less than 0.002 mm in diameter), high water retention, strong nutrient accumulation capacity, and low aeration. Clay soil can provide long-term moisture and abundant nutrients to the roots of *Striga asiatica* through its high water retention and good cation exchange capacity, which is conducive to plant growth and the accumulation of medicinal components. However, the high viscosity and low aeration of clay soil may limit root expansion and lead to waterlogging and root hypoxia. Therefore, during cultivation, adding organic matter or sandy soil to improve the aeration and drainage of clay soil can further optimize the growth environment of *Striga asiatica*, promote its root development and enhance its medicinal components, and help improve the germination speed and quality of *Striga asiatica*.

[0038] It's understandable that culturing *Striga asiatica* seeds in clean water for 5 days promotes water absorption, activates metabolic activity within the embryo, and prepares for germination. During this period, the seeds swell through water absorption, the seed coat softens, and enzymatic reactions within the seed gradually begin, triggering the germination mechanism. The 5-day water culture period ensures that the seeds absorb sufficient water and fully activate their biological functions.

[0039] It is understandable that in the early stages of germination, a dark environment helps the seeds of *Striga asiatica* to successfully enter the germination state. This is because germination of *Striga asiatica* seeds may be inhibited under light conditions. A dark environment simulates the soil conditions under natural conditions, avoiding light interference with the regulation of hormones and enzymes during seed germination and promoting physiological changes within the seed.

[0040] It is understandable that water temperature has a direct impact on the rate of water absorption and germination of seeds. 30 degrees Celsius is a suitable temperature for the water absorption and metabolic activity of *Striga asiatica* seeds. Providing a suitable physiological temperature can accelerate the transition of seeds from dormancy to an active metabolic stage. This temperature can activate enzymatic reactions inside the seed, promote intracellular metabolism, and enhance the seed's germination ability.

[0041] It is understandable that strigolactone is a sesquiterpene lactone compound extracted from the strigolactone plant. Hormone solutions containing strigolactone can regulate seed cell division, elongation, and other physiological functions. 5*10 -6 The mol / L concentration was the optimized concentration obtained by our team after multiple experiments. It effectively promotes seed germination and subsequent seedling growth without causing adverse effects due to drug overdose. The solution temperature was set at 34℃, which is suitable for the chemical activity of strigolactone and provides a warm environment for seed germination, further activating the internal growth process of the seed and further promoting the germination and seedling growth of *Striga asiatica* seeds in the soil environment.

[0042] It is understandable that sterile water is obtained by treating water containing bacterial colonies through physical methods. This can be achieved by using steam, UHT heat, ultraviolet sterilization, ozone, and physical filtration to kill and filter microorganisms in the water. Using sterile water to wash seeds that have been disinfected with sodium hypochlorite solution or alcohol can remove any residual sodium hypochlorite solution or alcohol from the seeds, thus preventing the embryo from being affected.

[0043] Example 1 describes a method for cultivating *Striga asiatica* in pots. A 1:1 mixture of *Striga asiatica* habitat soil (clay) and sand is selected as the substrate. Corn and crabgrass are chosen as host plants. Mature *Striga asiatica* seeds are first cultured in water under darkness, then cultured in a hormone solution containing strigolactones under darkness. After culturing, the *Striga asiatica* seeds are washed and inoculated around the roots of the host plants. This method effectively promotes the germination of *Striga asiatica* seeds in the soil and enhances their activity in the early growth stages, allowing them to fully absorb water during cultivation. This significantly shortens the germination time of *Striga asiatica* in soil, achieving rapid germination in soil-grown *Striga asiatica*.

[0044] In Example 1, the *Striga asiatica* cultured produced seedlings that emerged relatively quickly and grew vigorously, although the number of seedlings was moderate. Seedlings were visible emerging from the soil after 17 days. Figure 2 It is clearly visible that the seedlings, after 19 days, exhibit the rudimentary form of *Striga asiatica* plants. Figure 3 It is clearly visible that the plant flowers after 33 days. Figure 4 It is evident that the plants bear fruit after 40 days, and the *Striga asiatica* plants are in good health.

[0045] Example 2

[0046] A method for cultivating *Striga asiatica* in pots is provided, including the following steps:

[0047] Step A: Select a 1:1 mixture of potting soil and sand as the substrate. The potting soil composition is as follows: coconut coir 45%, peat moss 25%, corn cob residue 5%, xylose residue 5%, wood ash 5%, wheat bran 5%, Bacillus subtilis 3%, humic acid 3%, and perlite 4%. Select corn, crabgrass, and goosegrass as host plants and cultivate them to 12cm in the 1:1 mixture of potting soil and sand.

[0048] Step B: Take mature *Striga asiatica* seeds and cultivate them in clean water for 3 days in a dark environment at a water temperature of 32 degrees Celsius. After removing them, cultivate them in a hormone solution for 6 days in a dark environment at a hormone solution temperature of 35 degrees Celsius. Mature seeds are plump and have a brownish-black seed coat. The hormone solution concentration is 5*10. -6 mol / L strigolactone solution;

[0049] Step C: Take out the seeds of *Striga asiatica* and wash them with sterile water. Inoculate the washed seeds of *Striga asiatica* around the roots of the host plant.

[0050] Step D: Perform routine watering management on the Strigolactone seeds.

[0051] It is understood that the nutrient soil used in this embodiment, through scientific formulation of component ratios, can provide plants with balanced moisture, nutrients and aeration, and is particularly suitable for the cultivation and growth optimization of various crops or medicinal plants; the components and ratios of the nutrient soil in this embodiment are not strictly fixed and can be flexibly adjusted according to needs.

[0052] The potted cultivation method for *Striga asiatica* described in Example 2 above involves selecting a 1:1 mixture of nutrient soil and sand as the substrate, and choosing corn, crabgrass, and goosegrass as host plants. First, mature *Striga asiatica* seeds are cultured in water under darkness. Then, they are cultured in a hormone solution containing strigolactones under darkness. After culturing, the *Striga asiatica* seeds are washed and inoculated around the roots of the host plants. This method maximizes the seedling survival rate of *Striga asiatica* seeds without damaging them.

[0053] The *Striga asiatica* cultured in Example 2 developed well, with rapid and abundant seedling emergence; seedlings were visible emerging from the soil within 18 days. Figure 5 It is evident that the 20-day-old *Striga asiatica* plant is beginning to take shape. Figure 6 It is clearly visible that the plant will flower after 30 days. Figure 7 It is clear that the plants bear fruit after 38 days.

[0054] Example 3

[0055] A method for cultivating *Striga asiatica* in pots is provided, including the following steps:

[0056] Step A: Select a 1:1:1 mixture of potting soil, sand, and perlite as the substrate. The potting soil composition is as follows: coconut coir 45%, peat moss 25%, corn cob residue 5%, xylose residue 5%, wood ash 5%, wheat bran 5%, Bacillus subtilis 3%, humic acid 3%, and perlite 4%. Select corn, crabgrass, and goosegrass as host plants and cultivate them to 10cm in the soil (clay) substrate of the *Striga asiatica* habitat.

[0057] Step B: Take mature *Striga asiatica* seeds and cultivate them in clean water for 3 days in a dark environment at a water temperature of 30 degrees Celsius. After removal, disinfect the seeds with a 3% sodium hypochlorite solution for 3 minutes. Then, cultivate the seeds in a hormone solution for 3 days in a dark environment at a hormone solution temperature of 33 degrees Celsius. Mature seeds are plump with a brownish-black seed coat. The hormone solution concentration is 5*10... -8 mol / L strigolactone solution;

[0058] Step C: Take out the seeds of *Striga asiatica* and wash them with sterile water. Inoculate the washed seeds of *Striga asiatica* around the roots of the host plant.

[0059] Step D: Perform routine watering management on the Strigolactone seeds.

[0060] In Example 3, after removing the *Striga asiatica* seeds from clean water, they were disinfected with a 3% sodium hypochlorite solution for 3 minutes, and then cultured in a hormone solution. This method has the advantages of removing pathogens from the seed surface, dissolving the waxy coating on the seed surface, improving the germination rate, and preventing mold growth. At the same time, the disinfection process is fast and efficient and does not damage the seeds. This method can greatly improve the robustness of *Striga asiatica* seedlings and improve the quality of *Striga asiatica* plants while ensuring the number and speed of seedling emergence.

[0061] The *Striga asiatica* cultured in Example 3 developed well, and seedlings emerged from the soil after 14 days. Figure 8 It is evident that the 16-day-old *Striga asiatica* is beginning to take shape as a plant. Figure 9 It is clearly visible that the plant flowers after 35 days. Figure 10 It is evident that the plants bear fruit after 43 days, with a large number of seedlings emerging, and the plants are in good health.

[0062] Through the above three examples, good *Striga asiatica* plants were obtained through pot cultivation. Examples 1, 2, and 3 all involved culturing *Striga asiatica* seeds in water and a hormone solution containing strigolactone for different number of days, effectively promoting seed germination in the soil and enhancing activity in the early growth stages. Examples 2 and 3 incorporated nutrient soil into the culture medium to maximize the seedling rate without damaging the seeds. Example 3 used a 3% sodium hypochlorite solution to disinfect the *Striga asiatica* seeds for 3 minutes before culturing them in the hormone solution. This method effectively removes pathogens from the seed surface, dissolves the waxy layer that affects germination, improves the germination rate, and prevents mold growth. Furthermore, the disinfection process is rapid, efficient, and does not damage the seeds.

[0063] The following are some comparative examples for Example 1, Example 2 and Example 3 respectively:

[0064] The pre-culture process for *Striga asiatica* cultivation in Example 1 was modified, including the procedure and hormone solution concentration, to form the following three corresponding comparative examples 1, 2, and 3:

[0065] Comparative Example 1

[0066] Compared to Example 1, the *Striga asiatica* seeds were cultured in water but not in a hormone solution containing strigolactones, as detailed below:

[0067] Step A: Select a 1:1 mixture of soil (clay) and sand from the habitat of *Striga asiatica*, and select corn and crabgrass as host plants. Cultivate corn and crabgrass in the soil (clay) substrate from the habitat of *Striga asiatica* until they grow to 15cm.

[0068] Step B: Take mature *Striga asiatica* seeds and place them in clean water for 5 days in a dark environment at a water temperature of 30 degrees Celsius. Mature seeds are those that are plump and have a brownish-black seed coat.

[0069] Step C: Take out the seeds of *Striga asiatica* and wash them with sterile water. Inoculate the washed seeds of *Striga asiatica* around the roots of the host plant.

[0070] Step D: Perform routine watering management on the Strigolactone seeds.

[0071] In Comparative Example 1, *Striga asiatica* seeds were cultured in plain water without being placed in a hormone solution containing strigolactone. After 50 days, *Striga asiatica* seedlings emerged, and the development rate of the cultured seedlings was extremely low. This indicates that the strigolactone solution plays an important role in breaking the dormancy of *Striga asiatica* seeds and promoting their germination.

[0072] Comparative Example 2

[0073] Compared to Example 1, after culturing the *Striga asiatica* seeds in clean water, they were placed in 5*10... -2 The culture was carried out in a mol / L strigolactone solution, and the other procedures were the same.

[0074] Comparative Example 2 uses 5*10 -2 When strigolactone solution was used as a hormone solution to pre-culture strigolactone seeds, no strigolactone seedlings were observed to emerge after 60 days. This indicates that the high concentration of strigolactone solution had a destructive effect on the strigolactone seeds, causing them to die and fail to germinate.

[0075] Comparative Example 3

[0076] Compared to Example 1, instead of culturing the seeds in water, the seeds were directly placed in a hormone solution containing strigolactones for culturing, while the rest of the process remained the same.

[0077] In Comparative Example 3, instead of culturing the seeds of *Striga asiatica* in water, they were directly placed in a hormone solution containing strigolactone. It took 27 days for the seedlings to emerge from the soil. This shows that water plays a key role in activating the seeds, stimulating metabolic activity within the seed embryo, and preparing them for germination.

[0078] As can be seen from Example 1 and Comparative Examples 1, 2, and 3, compared to culturing *Striga asiatica* seeds in water without further culturing them in a hormone solution containing strigolactone, and then directly inoculating them around the roots of the host plant, the method used in Example 1 significantly shortens the germination rate of *Striga asiatica* seeds, resulting in a larger number of seedlings. Seedlings can be seen emerging from the soil in 17 days, showing the initial shape of *Striga asiatica* plants in 19 days, flowering in 33 days, and fruiting in 40 days. The *Striga asiatica* plants are in good health.

[0079] By changing the composition and ratio of the matrix in Example 2, the following comparative examples 4 and 5 were formed:

[0080] Comparative Example 4

[0081] Compared to Example 2, the host plant was planted using a 1:1 mixture of ordinary soil and sand, with all other processes remaining the same.

[0082] Comparative Example 4 used a mixture of ordinary soil and sand as the substrate, without the nutrient soil used in the examples. The host plants grown in this way grew slower. Compared with Example 2, it took an extra 1-2 weeks for the host plants to grow to 12cm. In addition, the seedlings of *Striga asiatica* also germinated more slowly. Seedlings could be seen emerging from the soil in 24 days, flowering in 40 days, and fruiting in 48 days. The number of seedlings was also significantly reduced.

[0083] Comparative Example 5

[0084] Compared to Example 2, the host plant was planted using a 4:1 mixture of nutrient soil and sand, with all other processes remaining the same.

[0085] In Comparative Example 5, the host plant was planted using a 4:1 mixture of nutrient soil and sand. The seedling rate of *Striga asiatica* seeds was reduced, and the seedling speed was also slower. This was because the soil contained less sand than in Example 2, which reduced the permeability of the substrate. As a result, oxygen could not effectively reach the roots of the seeds, and the seeds could not germinate successfully.

[0086] As can be seen from Examples 2 and Comparative Examples 4 and 5, selecting a strictly proportioned 1:1 mixture of nutrient soil and sand as the substrate can maximize the seedling rate of *Striga asiatica* seeds without damaging them.

[0087] The concentration and type of disinfectant in Example 3 were changed, as well as the light and temperature conditions, to form the following comparative examples 6, 7, and 8:

[0088] Comparative Example 6

[0089] Compared to Example 3, the seeds of *Striga asiatica* were disinfected with a 10% sodium hypochlorite solution, while the other processes remained the same.

[0090] Comparative Example 6 used a 10% sodium hypochlorite solution to disinfect the seeds of *Striga asiatica*. After 50 days, no *Striga asiatica* seedlings were observed to emerge. This indicates that the high concentration of sodium hypochlorite solution had a destructive effect on the *Striga asiatica* seeds, causing them to die and preventing germination.

[0091] Comparative Example 7

[0092] Compared to Example 3, the seeds of *Striga asiatica* were disinfected with a 3% hydrogen peroxide solution, while the other procedures remained the same.

[0093] Comparative Example 7 used a 3% hydrogen peroxide solution to disinfect the seeds of *Striga asiatica*. No germination was observed in the seeds, indicating that the 3% hydrogen peroxide solution inhibits the germination of *Striga asiatica* seeds or damages them.

[0094] Comparative Example 8

[0095] Compared with Example 3, Comparative Example 8 used different light environments and temperatures to pretreat the seeds of *Striga asiatica*, while other steps were the same. The specific treatment effects are shown in the table below:

[0096] Table 1: Number of days for *Striga asiatica* seedlings to emerge under different culture conditions

[0097]

[0098]

[0099] Comparative Example 8: The seeds of *Striga asiatica* were cultivated under different light conditions and temperatures, and the number of days it took for the seedlings to emerge varied considerably.

[0100] Compared with Comparative Example 8, Example 3, which used a dark environment culture in clean water at a temperature of 30 degrees Celsius and a dark environment culture in a hormone solution at a temperature of 33 degrees Celsius, showed significantly better results than other methods.

[0101] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0102] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. A method for cultivating *Striga asiatica* in pots, characterized in that, The method includes: Step A: Select a 1:1:1 mixture of potting soil, sand, and perlite as the substrate. The potting soil composition is as follows: coconut coir 45%, peat moss 25%, corn cob residue 5%, xylose residue 5%, wood ash 5%, wheat bran 5%, Bacillus subtilis 3%, humic acid 3%, and perlite 4%. Select corn, crabgrass, and goosegrass as host plants and cultivate them to 10cm in the 1:1:1 mixture of potting soil, sand, and perlite. Step B: Take mature *Striga asiatica* seeds and cultivate them in clean water for 3 days in a dark environment at a water temperature of 30 degrees Celsius. After removal, disinfect the seeds with a 3% sodium hypochlorite solution for 3 minutes. Then, cultivate the seeds in a hormone solution for 3 days in a dark environment at a hormone solution temperature of 33 degrees Celsius. Mature seeds are plump and have a brownish-black seed coat. The hormone solution is... strigolactone solution; Step C: Take out the seeds of *Striga asiatica* and wash them with sterile water. Inoculate the washed seeds of *Striga asiatica* around the roots of the host plant. Step D: Perform routine watering management on the Strigolactone seeds.

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