Biosafety medium and small animal infection transmission device

Abstract

The invention relates to a biosafety type medium and small animal infection transmission device, and relates to the technical field of infection transmission experiment devices.The biosafety type medium and small animal infection transmission device comprises a toxicity attacking closed box and an aerosol contamination closed box, the air inlet end of the toxicity attacking closed box is sequentially communicated with a first dryer and a first efficient filter, and the air outlet end of the toxicity attacking closed box is communicated with the air inlet end of the aerosol contamination closed box; the air outlet end of the aerosol contamination closed box is communicated with an air extracting pump; the toxicity attacking closed box body and the aerosol contamination closed box body are each provided with a temperature, humidity, oxygen and ammonia detection controller and a dehumidifier, and the temperature, humidity, oxygen and ammonia detection controllers are configured to be used for detecting environmental parameters such as temperature, humidity, oxygen and ammonia in the toxicity attacking closed box body or the aerosol contamination closed box body and can automatically adjust the air suction rate of the air suction pump according to the oxygen-ammonia ratio. By arranging the transmission infection device capable of carrying out an aerosol transmission infection verification experiment or a droplet transmission infection and aerosol transmission infection simultaneous verification experiment, the research requirements of aerosol transmission and droplet transmission infection mechanisms of pathogenic microorganisms are met.

Description

Technical Field

[0001] The present application relates to the field of infection transmission experimental devices, and in particular to a biosafety infection transmission device for small and medium-sized animals. Background Art

[0002] At present, in order to make a rapid and accurate diagnosis of infectious diseases, it is often necessary to conduct a comprehensive inspection and analysis of pathogenic microorganisms. Inhalation toxicology is a science that studies the various conditions, laws and mechanisms of the occurrence, development and elimination of the toxic effects of inhalable substances on the body, as well as the risk assessment of inhalable substances.

[0003] In existing research, it is necessary to conduct experiments on healthy animals and animals with pathogens together, and to infect healthy animals through the exhaled droplets and aerosols of pathogenic animals under certain environmental conditions (such as humidity, temperature, etc.), conduct transmission and infection experiments between animals, and then study the transmission and infection mechanism of viral microorganisms.

[0004] In order to meet the above experimental needs, the applicant designed a biosafety small and medium-sized animal transmission infection device. Summary of the invention

[0005] In view of the above problems, the present application is proposed to provide a biosafety device for transmitting infection to small and medium-sized animals that overcomes the above problems or at least partially solves the above problems.

[0006] The biosafety small and medium-sized animal infection transmission device provided in this application adopts the following technical solutions: A biosafety type small and medium-sized animal transmission infection device, comprising a closed box for placing infected animals and an aerosol infection closed box for placing healthy animals, wherein the air inlet end of the closed box is connected to a first dryer and a first high-efficiency filter in sequence, and the air outlet end is connected to the air inlet end of the aerosol infection closed box, and the air outlet end of the aerosol infection closed box is connected to an air pump; The poison attack closed box and the aerosol poisoning closed box are both provided with a temperature, humidity, oxygen and ammonia detection controller and a dehumidifier. The temperature, humidity, oxygen and ammonia detection controller is configured to detect environmental parameters such as temperature, humidity, oxygen and ammonia in the poison attack closed box or the aerosol poisoning closed box, and can automatically adjust the suction rate of the vacuum pump according to the oxygen-ammonia ratio.

[0007] Optionally, a particle separator is provided between the air outlet of the poison attack closed box and the air inlet of the aerosol poisoning closed box.

[0008] Optionally, a droplet infection closed box is provided between the particle separator and the infection closed box, and the droplet infection closed box is also used to place healthy animals. The infection closed box and the droplet transmission closed box are openly connected, and the infected animals placed in the infection closed box and the healthy animals placed in the droplet infection closed box are non-contact isolated.

[0009] Optionally, a second high-efficiency filter, a first muffler and a first solenoid valve are sequentially connected between the aerosol contamination closed box and the vacuum pump.

[0010] Optionally, a two-way valve is provided at the air outlet of the vacuum pump, and one port of the two-way valve is connected to the poison-attacking closed box, and the other port is connected to a third high-efficiency filter, a second muffler and a second solenoid valve in sequence.

[0011] Optionally, the air outlet of the vacuum pump is connected to the poison attack closed box, and a sodium peroxide reaction container is provided between the air inlet of the vacuum pump and the aerosol poisoning closed box, and sodium peroxide and manganese dioxide are placed in the sodium peroxide reaction container.

[0012] Optionally, a second dryer is provided between the attack closed box and the aerosol infection closed box.

[0013] Optionally, a third dryer is provided between the vacuum pump and the closed attack box.

[0014] Optionally, the attack closed box and the aerosol infection closed box are both connected and provided with a pressure differential gauge and a sampling tube.

[0015] In summary, the present application includes the following beneficial technical effects: The present application meets the research needs of aerosol transmission and droplet transmission infection mechanisms of pathogenic microorganisms by setting up a biosafety small and medium-sized animal transmission infection device that can conduct aerosol transmission infection verification experiments or droplet transmission infection and aerosol transmission infection simultaneous verification experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural diagram of Example 1 of the present application.

[0017] Figure 2 It is a structural diagram of Example 2 of the present application.

[0018] Figure 3 It is a structural diagram of Example 3 of the present application.

[0019] Figure 4 It is a structural diagram of Example 4 of the present application.

[0020] Figure 5It is a structural diagram of Example 5 of the present application.

[0021] Explanation of the reference numerals: 1. closed box for attacking poison; 2. closed box for aerosol contamination; 3. first dryer; 4. first high-efficiency filter; 5. second dryer; 6. second high-efficiency filter; 7. first muffler; 8. first solenoid valve; 9. vacuum pump; 10. differential pressure gauge; 11. sampling tube; 12. temperature and humidity oxygen and ammonia detection controller; 13. dehumidifier; 14. particle separator; 15. closed box for droplet contamination; 16. third high-efficiency filter; 17. second muffler; 18. second solenoid valve; 19. third dryer; 20. sodium peroxide reaction container. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiment is one embodiment of the present invention, not all embodiments. Based on the described embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Unless otherwise defined, technical or scientific terms used in the present invention shall have the common meanings understood by one having ordinary skills in the field to which the present invention belongs.

[0024] Embodiments 1-5 provide a biosafety device for the transmission of infection to small and medium-sized animals. Embodiment 1;

[0025] Reference Figure 1 The biosafety small and medium-sized animal transmission infection device includes a closed box for virus attack 1 and a closed box for aerosol infection 2. The closed box for virus attack 1 is used to place infected animals, that is, experimental animals carrying pathogenic microorganisms, and the closed box for aerosol infection 2 is used to place healthy animals.

[0026] The air inlet end of the poison attack closed box 1 is connected to the first dryer 3 and the first high-efficiency filter 4 in sequence, and the air outlet end is connected to the air inlet end of the aerosol poisoning closed box 2 through the second dryer 5; the air outlet end of the aerosol poisoning closed box 2 is connected to the second high-efficiency filter 6, the first silencer 7, the first solenoid valve 8 and the vacuum pump 9 in sequence.

[0027] During the experiment, under the action of the vacuum pump 9, the laboratory airflow is treated by the first dryer 3 and the first high-efficiency filter 4 and then enters the attacking closed box 1 and enters the aerosol infection closed box 2 through the second dryer 5. In the process of the airflow flowing from the attacking closed box 1 into the aerosol infection closed box 2, the pathogenic microorganisms carried by the infected animals are transmitted from the attacking closed box 1 to the aerosol infection closed box 2, thereby realizing the transmission and infection of healthy animals. When the airflow flows out from the outlet of the aerosol infection closed box 2, the second high-efficiency filter 6 filters it again to ensure the cleanliness of the airflow finally discharged into the laboratory environment and avoid polluting the laboratory environment.

[0028] In order to facilitate the observation of the negative pressure conditions of the poison attack closed box 1 and the aerosol poisoning closed box 2 and to take samples from the inside of the two, a pressure differential gauge 10 and a sampling tube 11 are provided for observing the negative pressure conditions of the poison attack closed box 1 and the aerosol poisoning closed box 2.

[0029] In order to detect environmental parameters such as temperature, humidity, oxygen, and ammonia in the closed box 1 for attacking poison and the closed box 2 for aerosol infection, the closed box 1 for attacking poison and the closed box 2 for aerosol infection are also provided with a temperature, humidity, oxygen, and ammonia detection controller 12, and the temperature, humidity, oxygen, and ammonia detection controller 12 can also automatically adjust the suction rate of the suction pump 9 according to the oxygen-ammonia ratio (i.e., the ratio of oxygen and ammonia concentrations) to ensure the normal concentration of oxygen in the closed box 1 for attacking poison and the closed box 2 for aerosol infection. At the same time, in order to further ensure the dryness of the airflow and prevent aerosols from agglomerating during the propagation process, resulting in a reduction in the propagation concentration and affecting the propagation efficiency, the closed box 1 for attacking poison and the closed box 2 for aerosol infection are also provided with a dehumidifier 13. Embodiment 2;

[0030] refer to Figure 2 The difference from Example 1 is that the attack closed box 1 and the aerosol infection closed box 2 are connected through a particle separator 14. The particle separator 14 separates and screens the aerosol particle size entering the aerosol infection closed box 2 through the impact principle. Large particles are captured by the particle separator 14, and small particles enter the aerosol infection closed box 2 through the particle separator 14. Since particles of different diameters can reach different healthy animal target organs, the healthy animal target organs that the aerosol can reach can be controlled by controlling the aerosol particle diameter, and the pathogenicity and characteristics of pathogens on different target organs are studied. Embodiment 3;

[0031] refer to Figure 3The difference from Example 2 is that a droplet infection closed box 15 is connected between the virus attack closed box 1 and the particle separator 14, and the droplet infection closed box 15 is also used to place healthy animals, and the virus attack closed box 1 and the droplet infection closed box 15 are openly connected, that is, the two are isolated by wire mesh, fences, etc., so that the infected animals set in the virus attack closed box 1 and the droplet infection closed box 15 are non-contact isolated, so that pathogenic microorganisms can infect healthy animals through droplets and spread by droplets.

[0032] After the animals in the droplet-contaminated closed box 15 are infected, the pathogenic microorganisms enter the aerosol-contaminated closed box 2 through the particle separator 14, and infect the healthy animals in the aerosol-contaminated closed box 2 through aerosol transmission. This setting can simultaneously realize experimental verification of droplet transmission and aerosol transmission. Embodiment 4;

[0033] refer to Figure 4 The difference from Example 1 is that the air inlet end of the vacuum pump 9 is directly connected to the aerosol contamination closed box 2, and the air outlet end is provided with a two-way valve. One port of the two-way valve is connected to the aerosol contamination closed box 1 through the third dryer 19, and the other port is connected to the third high-efficiency filter 16, the second muffler 17 and the second solenoid valve 18 in sequence.

[0034] This method can realize partial circulation of airflow in the attacking closed box 1 and the aerosol infection closed box 2, which helps to increase the concentration of pathogenic microorganisms in the aerosol, thereby helping to improve the efficiency of aerosol transmission infection, and can ensure the normal oxygen concentration in the attacking closed box 1 and the aerosol infection closed box 2 during a long period of infection.

[0035] In the actual implementation process, this embodiment can also be the same as embodiment 1 or embodiment 2, that is, the poison attack closed box 1 and the aerosol poisoning closed box 2 are connected through the particle separator 14 or a droplet poisoning closed box 15 is set between the particle separator 14 and the poison attack closed box 1. Embodiment 5;

[0036] refer to Figure 5 The difference from Example 1 is that the air outlet end of the vacuum pump 9 is directly connected to the attacking closed box 1 through the third dryer 19, and the air inlet end is connected to the aerosol infection closed box 2 through the sodium peroxide reaction container 20. Sodium peroxide and manganese dioxide are placed in the sodium peroxide reaction container 20. Sodium peroxide can react with carbon dioxide or water to generate oxygen under the catalytic action of manganese dioxide. While realizing the circulation of airflow in the attacking closed box 1 and the aerosol infection closed box 2, the normal oxygen concentration of the attacking closed box 1 and the aerosol infection closed box 2 can be guaranteed, thereby effectively improving the speed of aerosol transmission infection.

[0037] In the actual implementation process, this embodiment can also be the same as Example 1 or Example 2, that is, the poison attack closed box 1 and the aerosol poisoning closed box 2 are connected through the particle separator 14 or a droplet poisoning closed box 15 is set between the particle separator 14 and the poison attack closed box 1.

[0038] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. Biosafety small and medium-sized animal transmission infection device, characterized by: It comprises a closed box (1) for placing infected animals and an aerosol poisoning closed box (2) for placing healthy animals, wherein the air inlet of the closed box (1) is connected to a first dryer (3) and a first high-efficiency filter (4) in sequence, and the air outlet is connected to the air inlet of the closed box (2), and the air outlet of the closed box (2) is connected to an air pump (9); The poison attack closed box (1) and the aerosol poisoning closed box (2) are both provided with a temperature, humidity, oxygen and ammonia detection controller (12) and a dehumidifier (13); the temperature, humidity, oxygen and ammonia detection controller (12) is configured to detect environmental parameters such as temperature, humidity, oxygen and ammonia in the poison attack closed box (1) or the aerosol poisoning closed box (2), and can automatically adjust the suction rate of the suction pump (9) according to the oxygen-ammonia ratio.

2. The biosafety small and medium-sized animal transmission and infection device according to claim 1 is characterized by: A particle separator (14) is provided between the air outlet of the poison attack closed box (1) and the air inlet of the aerosol poisoning closed box (2).

3. The biosafety small and medium-sized animal transmission infection device according to claim 2 is characterized by: A droplet infection closed box (15) is provided between the particle separator (14) and the infection closed box (1), and the droplet infection closed box (15) is also used to place healthy animals. The infection closed box (1) and the droplet transmission closed box are openly connected, and the infected animals placed in the infection closed box (1) and the healthy animals placed in the droplet infection closed box (15) are non-contact isolated.

4. The biosafety small and medium-sized animal transmission infection device according to any one of claims 1 to 3, characterized in that: A second high-efficiency filter (6), a first silencer (7) and a first electromagnetic valve (8) are sequentially connected between the aerosol poisoning closed box (2) and the air extraction pump (9).

5. The biosafety small and medium-sized animal transmission infection device according to claim 1, characterized in that: A two-way valve is provided at the air outlet of the vacuum pump (9), and one of the ports of the two-way valve is connected to the poison-attacking closed box (1), and the other port is connected to a third high-efficiency filter (16), a second muffler (17) and a second solenoid valve (18) in sequence.

6. The biosafety small and medium-sized animal transmission infection device according to claim 1 is characterized by: The air outlet of the vacuum pump (9) is connected to the poison attacking closed box (1), and a sodium peroxide reaction container (20) is provided between the air inlet of the vacuum pump (9) and the aerosol poisoning closed box (2), and sodium peroxide and manganese dioxide are placed in the sodium peroxide reaction container (20).

7. The biosafety small and medium-sized animal transmission infection device according to claim 1, 5 or 6, characterized in that: A second dryer (5) is provided between the attack closed box (1) and the aerosol infection closed box (2).

8. The biosafety small and medium-sized animal transmission infection device according to claim 5 or 6, characterized in that: A third dryer (19) is provided between the vacuum pump (9) and the closed attack box (1).

9. The biosafety small and medium-sized animal transmission infection device according to claim 1, characterized in that: The poison attack closed box (1) and the aerosol poisoning closed box (2) are both connected and provided with a pressure differential gauge (10) and a sampling tube (11).

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