Application and method for incrementally expressing bombyx mori peptidoglycan recognition protein BmPGRP-L1 in breeding artificial feed adaptive bombyx mori varieties

By incrementally expressing BmPGRP-L1 or knocking out the gene in the midgut of silkworms, using CRISPR/Cas9 technology, the problem of insufficient individual quality and disease resistance of silkworms with artificial feed is solved, and the adaptability of silkworms to artificial feed is significantly improved. and economic traits.

CN119453150APending Publication Date: 2025-02-18GERMPLASM INNOVATION GRAND SCIENCE CENTER OF WESTERN CHINA (CHONGQING) SCIENCE CITY +1
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Patent Information

Application Number
CN202411608276.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-12
Publication Date
2025-02-18

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Abstract

The invention discloses an application and a method of incremental expression of bombyx mori peptidoglycan recognition protein BmPGRP-L1 in artificial feed adaptive bombyx mori variety cultivation, discovers that bombyx mori PGRP-L1 is located on a midgut inner side cell membrane, and constructs midgut incremental expression and systemic knock strains of bombyx mori BmPGRP-L1 genes. Incremental expression of the BmPGRP-L1 significantly improves expression of antibacterial peptide genes in midguts under physiological and pathological conditions, and significantly improves diversity and richness of intestinal flora; incremental expression individuals are significantly higher than wild individuals in larva stage weight and higher than wild individuals in survival rate. The whole cocoon weight, pupa weight, cocoon weight and cocoon shell rate of an incremental expression individual are obviously higher than those of a wild type. Therefore, the BmPGRP-L1 gene has important value in research and application of silkworm artificial feed adaptive variety breeding, and the gene can be expressed through transgenosis increment to improve the adaptability of silkworms to artificial feed.
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Citation Information

Patent Citations

  • Cultivated silkworm peptidoglycan identification protein gene

    CN101139599A

  • Peptidoglycan recognition protein encoding nucleic acids and methods of use thereof

    WO2003029401A2