A flow cytometry antibody microsphere packaging structure

Abstract

The application discloses a flow type antibody microsphere packaging structure, which comprises a tube body, antibody microspheres arranged at the bottom of the tube body, and a cover body used for sealing the tube body, wherein a hollow limiting part used for limiting the antibody microspheres is arranged at the bottom of the cover body and extends to the bottom of the tube body, and the hollow limiting part is connected with the bottom of the cover body through a rod body. The application can effectively fix the antibody microspheres and prevent displacement of the antibody microspheres, and meanwhile, through diversified limiting design and monitoring means, the storage safety and reliability are improved, so that the application has a wide application prospect.

Description

Technical Field

[0001] This invention belongs to the field of flow cytometry technology and discloses a flow cytometry antibody microsphere packaging structure. Background Technology

[0002] Antibody microspheres, as important biomaterials, have wide applications in drug delivery, immunoassay, and biosensing. Due to their tiny size (typically between 1 μm and 250 μm), antibody microspheres possess a large specific surface area, which can effectively improve drug bioavailability and targeting. However, antibody microspheres face numerous challenges in storage and transportation during practical applications, especially in storage devices such as flow cytometry tubes, where their small size and high flexibility make immobilization and stabilization particularly difficult.

[0003] Currently, many traditional antibody microsphere storage methods use a bottom iron plate or similar structure to immobilize the microspheres. While this method is simple, it has several significant drawbacks. First, the material of the iron plate may react with the antibody microspheres, leading to reduced activity and affecting subsequent experimental results. Second, the bottom iron plate cannot completely prevent the microspheres from shifting within the flow cytometer; random movement of the microspheres can result in inconsistent and poorly reproducible experimental data. Furthermore, traditional immobilization structures often increase the complexity of assembly and disassembly, making experimental operations and maintenance inconvenient. Summary of the Invention

[0004] To address the aforementioned issues, this invention provides a flow cytometry antibody microsphere packaging structure that effectively immobilizes antibody microspheres and prevents their displacement. Furthermore, through diverse containment designs and monitoring methods, it enhances the safety and reliability of storage.

[0005] The technical solution provided by this invention is as follows:

[0006] A flow cytometry antibody microsphere packaging structure includes a tube body, antibody microspheres placed at the bottom of the tube body, and a cap for sealing the tube body. The bottom of the cap body extends towards the bottom of the tube body and has a perforated limiting portion for limiting the antibody microspheres. The perforated limiting portion is connected to the bottom of the cap body by a rod.

[0007] In some implementations, the tube is a flow tube or a glass tube.

[0008] In some embodiments, the hollowed-out limiting part is claw-shaped, and the ends of several claws wrap and fix the antibody microspheres.

[0009] In some embodiments, the perforated limiting part is a mesh cover type, which covers and fixes the antibody microspheres.

[0010] In some embodiments, a protrusion is provided in the middle of the rod, and the outer periphery of the protrusion fits against the inner wall of the tube.

[0011] In some embodiments, a moisture-absorbing sheet is provided on the outer periphery of the protrusion, and the moisture-absorbing sheet has water absorption and color development lines.

[0012] In some implementations, the cover is a threaded cap or a plug.

[0013] In some implementations, the stopper is a rubber stopper or a wooden stopper.

[0014] In summary, the beneficial effects of the present invention are as follows:

[0015] (1) This invention can not only effectively fix antibody microspheres and prevent them from shifting, but also improve the safety and reliability of storage through diversified limiting design and monitoring methods, and has broad application prospects.

[0016] (2) The protrusion of the present invention can further seal the storage chamber of antibody microspheres, ensuring dryness and preventing antibody microspheres from getting damp.

[0017] (3) The present invention has a moisture-absorbing sheet with a raised outer periphery and a water absorption color line on the moisture-absorbing sheet. The color line changes from yellow to blue according to the amount of water absorbed, thus ensuring the long-term stability of the antibody microspheres by detecting the moisture status of the antibody microspheres in the tube. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the exploded structure of Embodiment 1 of the present invention;

[0019] Figure 2 This is a schematic diagram of the internal structure of Embodiment 2 of the present invention;

[0020] Figure 3 This is a schematic cross-sectional view of Embodiment 1 of the present invention;

[0021] Figure 4 This is a cross-sectional structural diagram of Embodiment 2 of the present invention.

[0022] The attached figures are labeled as follows:

[0023] 1. Tube body; 2. Antibody microspheres; 3. Cap body; 4. Hollowed-out limiting part; 5. Rod body; 6. Protrusion; 7. Moisture-absorbing sheet. Detailed Implementation

[0024] To enhance understanding of the present invention, the present invention will be further described in detail below with reference to embodiments and accompanying drawings. These embodiments are only used to explain the present invention and do not constitute a limitation on the scope of protection of the present invention.

[0025] Example 1

[0026] Please refer to Figure 1 , 3This embodiment provides a flow cytometry antibody microsphere packaging structure, including a tube body 1, a cap 3, and antibody microspheres 2. First, the antibody microspheres 2 are placed at the bottom of the tube body 1. To ensure that the antibody microspheres 2 do not shift during transportation or storage, the cap 3 is designed as a cap with a limiting structure, thereby further fixing the microspheres and sealing the tube body 1.

[0027] Tube 1 is a flow cytometer or glass tube, with high transparency as the primary material choice to ensure that researchers can clearly observe the storage status of the antibody microspheres inside the tube. The main body of tube 1 has a hollow cylindrical structure, and the bottom of tube 1 is designed with an arc shape to better accommodate and support the microspheres, preventing the microspheres from gathering in a certain position due to the irregular shape of the bottom.

[0028] The cap 3 is designed to ensure sealing and stability. A rod 5 is located at the bottom of the cap 3. The rod 5 is made of a sturdy yet lightweight material to reduce the weight burden inside the tube and to prevent deformation or breakage during the positioning of the antibody microspheres 2. The rod 5 extends towards the bottom of the tube 1, and its end connects to the hollowed-out positioning part 4. In this embodiment, the hollowed-out positioning part 4 is designed as a claw-shaped structure with eight claws evenly spaced, forming a stable enclosure around the antibody microspheres 2. The ends of the claws are curved to ensure that the antibody microspheres 2 are effectively wrapped and fixed, preventing them from falling off or shifting even during vigorous shaking or transportation. This avoids the microspheres moving or detaching from their initial position due to external forces or movement of the tube.

[0029] The cap 3 achieves a seal by engaging with the threaded top of the tube 1, ensuring that the internal microspheres are not affected by external air or moisture when tightened. This structure not only keeps the antibody microspheres 2 stable in the flow cytometer but also simplifies assembly and disassembly, facilitating rapid use and replacement by laboratory personnel.

[0030] Example 2

[0031] Please refer to Figure 2 , 4 This embodiment is based on Embodiment 1. By further modifying the structure of the rod 5, the storage quality of the antibody microspheres 2 is improved. Specifically:

[0032] In this embodiment, the hollow limiting part 4 is designed as a mesh cover. The mesh cover is semi-circular and covers the antibody microspheres 2. Its mesh aperture is smaller than the diameter of the antibody microspheres 2, which can effectively cover the antibody microspheres 2 and prevent them from displacing inside the tube.

[0033] In this embodiment, a cylindrical protrusion 6 is provided in the middle of the rod 5. The outer periphery of the protrusion 6 is fitted against the inner wall of the tube 1. The space below the protrusion 6 to the bottom of the tube 1 forms a storage chamber for the antibody microspheres 2. This design isolates the storage chamber for the antibody microspheres 2 from the external environment, forming an independent sealed environment, which greatly reduces the risk of damage to the performance of the antibody microspheres 2 due to changes in external humidity. Especially in experimental environments with high humidity, the design of the protrusion 6 can effectively reduce the contact between the antibody microspheres and the outside air, keeping the microspheres dry for a long time.

[0034] In this embodiment, a moisture-absorbing sheet 7 can also be provided on the outer periphery of the protrusion 6. The moisture-absorbing sheet 7 has a water absorption color development line, which changes from yellow to blue according to the amount of water absorbed. This is used to detect the moisture status of the antibody microspheres 2 inside the detection tube 1, ensuring the long-term stability of the antibody microspheres 2. If the moisture-absorbing sheet 7 changes color significantly, it indicates that the antibody microspheres 2 have become damp and should not be used. The other side of the moisture-absorbing sheet 7 can serve as an information label carrier, affixing product-related information, including the type of antibody microspheres 2, production batch, and expiration date, so that researchers can quickly identify the product information during use and avoid misuse or use after expiration.

[0035] The flow cytometry antibody microsphere 2 packaging structure of this application can not only effectively fix the antibody microsphere 2 and prevent its displacement, but also improve the safety and reliability of storage through diversified limiting design and monitoring methods.

[0036] It should be noted that implementations not shown or described in the accompanying drawings or the main text of the specification are all forms known to those skilled in the art and are not described in detail. Furthermore, the definitions of the elements and methods described above are not limited to the various specific structures, shapes, or methods mentioned in the embodiments.

[0037] It should also be noted that this document may provide examples of parameters containing specific values, but these parameters need not be exactly equal to the corresponding values, but can approximate the corresponding values ​​within acceptable error tolerances or design constraints. Directional terms mentioned in the embodiments, such as "up," "down," "front," "back," "left," "right," "inner," and "outer," are only for reference to the directions in the accompanying drawings and are not intended to limit the scope of protection of this application.

[0038] The foregoing description illustrates and describes preferred embodiments of the present invention. As previously stated, it should be understood that the present invention is not limited to the forms disclosed herein and should not be construed as excluding other embodiments. It can be used in various other combinations, modifications, and environments, and can be altered within the scope of the inventive concept described herein through the foregoing teachings or techniques or knowledge in related fields. Any modifications and variations made by those skilled in the art that do not depart from the spirit and scope of the present invention should be within the protection scope of the appended claims.

Claims

1. A flow cytometry antibody microsphere packaging structure, characterized in that, It includes a tube body (1), antibody microspheres (2) placed at the bottom of the tube body (1), and a cap (3) for sealing the tube body (1). The bottom of the cap (3) extends toward the bottom of the tube body (1) and is provided with a hollow limiting part (4) for limiting the antibody microspheres (2). The hollow limiting part is connected to the bottom of the cap (3) through a rod (5). The hollow limiting part (4) is claw-shaped, and the ends of several claws wrap and fix the antibody microspheres (2); The rod (5) has a protrusion (6) in the middle. The outer periphery of the protrusion (6) is attached to the inner wall of the tube (1). The space below the protrusion (6) to the bottom of the tube (1) forms a storage chamber for storing the antibody microspheres (2). The outer periphery of the protrusion (6) is provided with a moisture-absorbing sheet (7), and the moisture-absorbing sheet (7) has water-absorbing color lines.

2. The flow cytometry antibody microsphere packaging structure according to claim 1, characterized in that, The tube (1) is a flow tube or a glass tube.

3. The flow cytometry antibody microsphere packaging structure according to claim 1, characterized in that, The cover (3) is a threaded cover or a plug.

4. The flow cytometry antibody microsphere packaging structure according to claim 3, characterized in that, The plug is a rubber plug or a wooden plug.

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