一种腈水解酶突变体及其在酰胺合成中的应用
By site-directed mutagenesis of nitrile hydrolase Nit-0305, particularly the modification of amino acid residues at positions 51, 162, and 185, a nitrile hydrolase mutant with high efficiency in catalyzing the formation of corresponding amides from 2, 3, or 4-cyanopyridine was constructed. This solved the problems of low catalytic efficiency and numerous byproducts in existing nitrile hydrolases, achieving efficient and targeted amide formation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- BAI KAISHENG (SHANGHAI) BIOTECHNOLOGY CO LTD
- Filing Date
- 2023-08-11
- Publication Date
- 2026-07-17
AI Technical Summary
Existing nitrile hydrolases suffer from low catalytic efficiency, numerous byproducts, and poor stereoselectivity when catalyzing the synthesis of corresponding amides from 2,3, or 4-cyanopyridine, making it difficult to meet industrial requirements.
By performing site-directed mutagenesis on Nit-0305, a nitrile hydrolase derived from Cereibacter sphaeroides, particularly modifying amino acid residues at positions 51, 162, and 185, a nitrile hydrolase mutant with high catalytic activity was constructed, increasing amide production and regulating the ratio of main to byproducts.
It significantly improved the efficiency of catalytic formation of the corresponding amides by 2-cyanopyridine, 3-cyanopyridine and 4-cyanopyridine. The formation amounts of 2-pyridinecarboxamide, nicotinamide and isonicotinamide increased by 51 times, 23.7 times and 67 times respectively, achieving the effect of efficient and targeted amide formation.
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