一种通过操纵基因非编码区延缓细胞衰老的方法
By intervening in the selective polyadenylation (APA) pattern of the KIAA1549 gene using whole-genome CRISPRpas screening technology, the problem of the failure of existing technologies to effectively delay cell senescence was solved, and the improvement of cell senescence phenotype and proliferation capacity were achieved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- FUDAN UNIVERSITY
- Filing Date
- 2024-10-31
- Publication Date
- 2026-07-17
AI Technical Summary
Current technologies have not effectively utilized CRISPRpas to regulate cellular senescence, and methods to delay cellular senescence by manipulating non-coding regions of genes have not been reported.
Using whole-genome CRISPRpas screening technology, a gRNA library targeting the selective 3′UTR of a gene was designed and constructed. This library intervened in the selective polyadenylation (APA) pattern of the KIAA1549 gene, shortening the 3′UTR length and thus delaying cellular senescence.
It significantly improves cellular senescence phenotype, enhances cell proliferation, reduces the proportion of SA-β-gal positive cells, increases the proportion of MKI67 positive cells, downregulates CDKN1A expression, regulates LMNB1 and IL1A expression, and prevents carcinogenesis risk.
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Figure CN119464388B_ABST