3D protein mutated A-type Seneca recombinant virus strain as well as construction and application of 3D protein mutated A-type Seneca recombinant virus strain

CN119464505APending Publication Date: 2025-02-18LANZHOU VETERINARY RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES(LANZHOU BRANCH CENTER OF CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENTER)
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Patent Information

Application Number
CN202411589773.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-08
Publication Date
2025-02-18

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Abstract

The invention belongs to the technical field of biological genetic engineering, and particularly relates to a 3D protein mutated A-type Seneca recombinant virus strain as well as construction and application thereof. The invention firstly finds that down-regulation of RSAD1 can significantly inhibit replication of SVA, and RSAD1 as a target spot can be used for screening drugs for inhibiting SVA; secondly, the 398th amino acid of the SVA 3D protein is a key site of the host containing RSAD1 mediated oxidation modification, and after the 398th amino acid of the 3D protein is mutated into A, the stability of the 3D protein is influenced, the replication of the SVA is inhibited, and the virus titer is reduced; finally, the 398th amino acid of the 3D protein is mutated into A to construct and successfully save to obtain the SVA recombinant virus strain rSVA-3DM398A, and compared with wild type SVA, the rSVA-3DM398A has the advantages that the virulence is obviously reduced, the virulence is reduced, and the rSVA-3DM398A can be used as a recombinant vaccine strain and has a good application prospect.
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Citation Information

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