A method for fabricating a sensor, the sensor itself, and its application.

Abstract

The sensor preparation method provided in this application involves dispersing a sensitive material and a conductive material in a solvent and ultrasonically treating them to obtain a nanocomposite material solution. The nanocomposite material solution is then drop-cast onto the surface of a conductive substrate and dried to obtain the sensor. This method is simple, requires no complex equipment or stringent conditions, and significantly reduces production costs and energy consumption. Furthermore, the ratio of the sensitive material dispersion to the conductive material dispersion can be flexibly adjusted during the preparation process to optimize performance, meeting the required sensitivity and stability in menthone solutions of different concentrations. This results in superior detection accuracy and stability in menthone detection, representing an innovative solution that combines low cost and high performance. Additionally, this application also provides a sensor and its application.

Description

Technical Field

[0001] This application relates to the field of biological detection technology, and in particular to a method for preparing a sensor, the sensor itself, and its application. Background Technology

[0002] Menthone is a common biomarker used in the metabolism of antiviral and anti-inflammatory drugs, and as an important indicator of food quality and authenticity. Furthermore, menthone is also a biomarker for brain diseases such as epilepsy.

[0003] In existing technologies, the detection methods for menthone mainly focus on gas chromatography (GC) and liquid chromatography (LC). However, these methods suffer from a series of drawbacks, including high cost, complex operation, demanding sample pretreatment requirements, limited sensitivity, difficulty in real-time detection, and strict environmental requirements. These shortcomings significantly limit the detection efficiency and applicability of menthone in practical applications, necessitating the development of new menthone detection methods. Summary of the Invention

[0004] Therefore, it is necessary to provide a method for preparing a sensor, a sensor, and an application that can effectively improve the detection accuracy and stability of menthone, addressing the shortcomings of current menthone detection methods.

[0005] To solve the above problems, this application adopts the following technical solution:

[0006] One of the objectives of this application is to provide a method for fabricating a sensor, comprising the following steps:

[0007] Sensitive and conductive materials are dispersed in a solvent and ultrasonically treated to obtain a nanocomposite solution.

[0008] The sensor is obtained by drop casting the nanocomposite solution onto the surface of a conductive substrate and then drying it.

[0009] In some embodiments, the step of dispersing the sensitive material and the conductive material in a solvent and ultrasonically treating them to obtain a nanocomposite solution specifically includes the following steps:

[0010] A sensitive material is dispersed in a solvent to form a sensitive material dispersion, and a conductive material is dispersed in a solvent to form a conductive material dispersion. The concentration of the sensitive material dispersion is 1–50 mg / mL, and the concentration of the conductive material dispersion is 0.5–3 mg / mL.

[0011] The dispersions of the sensitive material and the conductive material, with a volume ratio of 0.2 to 1, are ultrasonically treated for 5 to 30 minutes to obtain a nanocomposite material solution.

[0012] In some embodiments, the sensitive material includes nano-oxides, which are not limited to oxides of Ni, Cu, Mn, Ti, Au, or Pt.

[0013] In some embodiments, the conductive material includes, but is not limited to, graphene, graphite, carbon nanotubes, or MXene.

[0014] In some embodiments, the solvent includes, but is not limited to, ethanol or deionized water.

[0015] In some embodiments, the step of drop-casting the nanocomposite solution onto the surface of a conductive substrate and then drying it to obtain the sensor specifically includes the following steps:

[0016] The sensor is obtained by drop casting the nanocomposite solution onto the surface of a conductive substrate and then physically drying it for 20 to 60 minutes. The conductive substrate includes, but is not limited to, nickel foam, nano-gold, nano-carbon, or platinum black. The physical drying includes, but is not limited to, an oven or a heating table.

[0017] In some embodiments, the step of activating the sensor is also included.

[0018] In some embodiments, the step of activating the sensor specifically includes:

[0019] The sensor is placed at the working electrode position of a three-electrode system and immersed in an activator for electrochemical scanning. After scanning, the sensor is cleaned and physically dried to obtain an activated sensor. The activator includes, but is not limited to, ferrous and / or ferric ions, potassium chloride and / or potassium ferricyanide, benzoquinone and / or hydroquinone. The electrochemical method includes, but is not limited to, CV, It, DPV, or LSV.

[0020] A second objective of this application is to provide a sensor prepared by any of the preparation methods described herein.

[0021] A third objective of this application is to provide an application of the aforementioned sensor in detecting menthone concentration.

[0022] In some embodiments, the detection range of menthone concentration is 0.2-5 mM.

[0023] The present application adopts the above technical solution, and its beneficial effects are as follows:

[0024] The sensor preparation method, sensor, and application provided in this application involve dispersing sensitive and conductive materials in a solvent and ultrasonically treating them to obtain a nanocomposite material solution. The nanocomposite material solution is then drop-cast onto a conductive substrate and dried to obtain the sensor. This method is simple, requires no complex equipment or stringent conditions, and significantly reduces production costs and energy consumption. Furthermore, the ratio of the sensitive material dispersion to the conductive material dispersion can be flexibly adjusted during the preparation process to optimize performance, meeting the required sensitivity and stability in menthone solutions of different concentrations. This results in superior detection accuracy and stability in menthone detection, representing an innovative solution that combines low cost and high performance. Attached Figure Description

[0025] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the description of the embodiments of this application or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1 The CV curve of the SPCE / CuO@MWCNTs sensor provided in the embodiments of this application in a mixed solution of KCl and K3[Fe(CN)6].

[0027] Figure 2 The sensor prepared in Example 1 of this application has detection curves (a) for different concentrations of menthone and fitting curves (b) for the peak current of the characteristic peak of menthone at 0.82V.

[0028] Figure 3 The sensor prepared in Example 2 of this application has detection curves (a) for different concentrations of menthone and fitting curves (b) for the peak current of the characteristic peak of menthone at 0.82V.

[0029] Figure 4 The sensor prepared in Example 3 of this application has detection curves (a) for different concentrations of menthone and fitting curves (b) for the peak current of the characteristic peak of menthone at 0.82V.

[0030] Figure 5 The sensor prepared in Example 4 of this application has detection curves (a) for different concentrations of menthone and fitting curves (b) for the peak current of the characteristic peak of menthone at 0.82V. Detailed Implementation

[0031] The embodiments of this application are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain this application, and should not be construed as limiting this application.

[0032] In the description of this application, it should be understood that the terms "upper", "lower", "horizontal", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0033] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "multiple" means two or more, unless otherwise explicitly specified.

[0034] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments.

[0035] The sensor fabrication method provided in this application embodiment specifically includes the following steps:

[0036] Step S110: Disperse the sensitive material and the conductive material in a solvent and sonicate to obtain a nanocomposite solution.

[0037] In some embodiments, the step of dispersing the sensitive material and the conductive material in a solvent and ultrasonically treating them to obtain a nanocomposite solution specifically includes the following steps:

[0038] A sensitive material is dispersed in a solvent to form a sensitive material dispersion, and a conductive material is dispersed in a solvent to form a conductive material dispersion. The concentration of the sensitive material dispersion is 1–50 mg / mL, and the concentration of the conductive material dispersion is 0.5–3 mg / mL.

[0039] The dispersions of the sensitive material and the conductive material, with a volume ratio of 0.2 to 1, are ultrasonically treated for 5 to 30 minutes to obtain a nanocomposite material solution.

[0040] In some embodiments, the sensitive material includes nano-oxides, which are not limited to oxides of Ni, Cu, Mn, Ti, Au, or Pt.

[0041] In some embodiments, the conductive material includes, but is not limited to, graphene, graphite, carbon nanotubes, or MXene.

[0042] In some embodiments, the solvent includes, but is not limited to, ethanol or deionized water.

[0043] It is understood that in this embodiment, ethanol or deionized water is used as a solvent, and sensitive materials and conductive materials are used as solutes to form a nanocomposite solution. By adjusting the ratio of sensitive materials and conductive materials, the performance is optimized to better meet the needs of practical applications.

[0044] Step S120: The nanocomposite solution is drop-cast onto the surface of a conductive substrate and dried to obtain the sensor.

[0045] In some embodiments, the step of drop-casting the nanocomposite solution onto the surface of a conductive substrate and then drying it to obtain the sensor specifically includes the following steps:

[0046] The sensor is obtained by drop casting the nanocomposite solution onto the surface of a conductive substrate and then physically drying it for 20 to 60 minutes. The conductive substrate includes, but is not limited to, nickel foam, nano-gold, nano-carbon, or platinum black. The physical drying includes, but is not limited to, an oven or a heating table.

[0047] In some embodiments, the step of activating the sensor is also included.

[0048] In some embodiments, the step of activating the sensor specifically includes:

[0049] The sensor is placed at the working electrode position of a three-electrode system and immersed in an activator for electrochemical scanning. After scanning, the sensor is cleaned and physically dried to obtain an activated sensor. The activator includes, but is not limited to, ferrous and / or ferric ions, potassium chloride and / or potassium ferricyanide, benzoquinone and / or hydroquinone. The electrochemical method includes, but is not limited to, CV, It, DPV, or LSV.

[0050] The sensor preparation method provided in the above embodiments of this application is simple and does not require complex equipment or strict conditions, which significantly reduces production costs and energy consumption. Moreover, the ratio of the sensitive material dispersion and the conductive material dispersion can be flexibly adjusted during the preparation process to optimize performance, so as to meet the required sensitivity and stability in menthone solutions of different concentrations. This results in superior detection accuracy and stability in menthone detection, making it an innovative solution that combines low cost and high performance.

[0051] The sensor prepared according to the above embodiments of this application can be used for the detection of menthone concentration.

[0052] Furthermore, the sensor provided in this embodiment has a detection sensitivity of 41.04 μA·mM in detecting the concentration of menthone. -1 ·cm -2 The detection concentration range is 0.2-5mM.

[0053] Please see Figure 1 The CV curves of the SPCE / CuO@MWCNTs sensor in a mixed solution of KCl and K3[Fe(CN)6] are shown below. Figure 1 As can be seen, the above sensor can exhibit good sensitivity and stability in menthol solutions of different concentrations.

[0054] The technical solutions described above in this application will be explained in detail below with reference to specific embodiments.

[0055] In the following embodiments, the concentration of the sensitive material dispersion is selected as 10 mg / mL, 5 mg / mL, or 1 mg / mL, and the concentration of the conductive material dispersion is selected as 2 mg / mL or 0 mg / mL. For specific embodiments, please refer to the following description.

[0056] Example 1

[0057] The sensor fabrication method provided in this embodiment is as follows:

[0058] CuO is dispersed in deionized water to form a sensitive material dispersion, and carbon nanotubes are dispersed in a solvent to form a conductive material dispersion. The concentration of the sensitive material dispersion is 10 mg / mL, and the concentration of the conductive material dispersion is 2 mg / mL.

[0059] The dispersions of the sensitive material and the conductive material, with a volume ratio of 1, were ultrasonically treated for 30 minutes to obtain a nanocomposite solution.

[0060] The sensor was obtained by drop casting the nanocomposite solution onto the surface of nickel foam and then physically drying it for 20 minutes.

[0061] Please see Figure 2 The images show the detection curves (a) of the sensor prepared in Example 1 for different concentrations of menthone and the fitting curve (b) for the peak current of the characteristic peak of menthone at 0.82V. Figure 2 The results show the effectiveness of the electrode prepared with 10 mg / mL CuO and 2 mg / mL carbon nanotubes in detecting menthone.

[0062] Example 2

[0063] The sensor fabrication method provided in this embodiment is as follows:

[0064] NiO is dispersed in deionized water to form a sensitive material dispersion, and carbon nanotubes are dispersed in a solvent to form a conductive material dispersion. The concentration of the sensitive material dispersion is 5 mg / mL, and the concentration of the conductive material dispersion is 2 mg / mL.

[0065] The dispersions of the sensitive material and the conductive material, with a volume ratio of 0.2, were ultrasonically treated for 50 minutes to obtain a nanocomposite solution.

[0066] The sensor was obtained by drop casting the nanocomposite solution onto a platinum black surface and then physically drying it for 60 minutes.

[0067] Please see Figure 3 The images show the detection curves (a) of the sensor prepared in Example 2 for different concentrations of menthone and the fitting curve (b) for the peak current of the characteristic peak of menthone at 0.82V. Figure 3 The results show the effectiveness of the electrode prepared with 5 mg / mL NiO and 2 mg / mL carbon nanotubes in the detection of menthone.

[0068] Example 3

[0069] The sensor fabrication method provided in this embodiment is as follows:

[0070] Cu2O is dispersed in deionized water to form a sensitive material dispersion, and carbon nanotubes are dispersed in a solvent to form a conductive material dispersion. The concentration of the sensitive material dispersion is 1 mg / mL, and the concentration of the conductive material dispersion is 2 mg / mL.

[0071] The dispersions of the sensitive material and the conductive material, with a volume ratio of 0.5, were ultrasonically treated for 10 minutes to obtain a nanocomposite solution.

[0072] The sensor was obtained by drop casting the nanocomposite solution onto the surface of nano-carbon and then physically drying it for 40 minutes.

[0073] Please see Figure 4 The images show the detection curves (a) of the sensor prepared in Example 3 for different concentrations of menthone and the fitting curve (b) for the peak current of the characteristic peak of menthone at 0.82V. Figure 4 The results show the effectiveness of the electrode prepared with 1 mg / mL Cu2O and 2 mg / mL carbon nanotubes in detecting menthone.

[0074] Example 4

[0075] The sensor fabrication method provided in this embodiment is as follows:

[0076] CuO was dispersed in deionized water and ultrasonicated for 10 min to form a sensitive material dispersion. This dispersion was then drop-cast onto a carbon nanotube surface and physically dried for 40 min to obtain the sensor. The concentration of the sensitive material dispersion was 5 mg / mL.

[0077] Please see Figure 5 The images show the detection curves (a) of the sensor prepared in Example 4 for different concentrations of menthone and the fitting curve (b) for the peak current of the characteristic peak of menthone at 0.82V. Figure 5 As can be seen, the electrode prepared with 5 mg / mL CuO alone failed to detect menthone (i.e., no oxidation peak appeared near the potential of 0.82 V).

[0078] The above are merely preferred embodiments of this application, and only specifically describe the technical principles of this application. These descriptions are only for explaining the principles of this application and should not be construed as limiting the scope of protection of this application in any way. Based on this explanation, any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application, as well as other specific embodiments of this application that can be conceived by those skilled in the art without creative effort, should be included within the scope of protection of this application.

Claims

1. Application of a sensor in detecting menthone concentration; The method for preparing the sensor includes the following steps: Sensitive and conductive materials are dispersed in a solvent and ultrasonically treated to obtain a nanocomposite solution. The sensor is obtained by drop casting the nanocomposite solution onto the surface of a conductive substrate and then drying it. The step of dispersing sensitive and conductive materials in a solvent and ultrasonically treating them to obtain a nanocomposite solution specifically includes the following steps: A sensitive material is dispersed in a solvent to form a sensitive material dispersion, and a conductive material is dispersed in a solvent to form a conductive material dispersion. The concentration of the sensitive material dispersion is 1~50 mg / mL, and the concentration of the conductive material dispersion is 0.5~3 mg / mL. The dispersions of the sensitive material and the conductive material, with a volume ratio of 0.2 to 1, are ultrasonically treated for 5 to 30 minutes to obtain a nanocomposite material solution. The sensitive material includes nano-oxides, which are oxides of Ni or Cu. The conductive material is carbon nanotubes; It also includes the step of activating the sensor; The activation step of the sensor specifically includes: The sensor is placed at the working electrode position of a three-electrode system and immersed in an activator for electrochemical scanning. After scanning, the sensor is cleaned and physically dried to obtain an activated sensor. The activator includes ferrous and / or ferric ions, potassium chloride and / or potassium ferricyanide, benzoquinone and / or hydroquinone, and the electrochemical method includes CV, It, DPV, or LSV.

2. The application of the sensor as described in claim 1 in detecting menthone concentration, characterized in that, The solvent includes ethanol or deionized water.

3. The application of the sensor as described in claim 1 in detecting menthone concentration, characterized in that, The step of drop-casting the nanocomposite solution onto the surface of a conductive substrate and then drying it to obtain the sensor specifically includes the following steps: The sensor is obtained by drop casting the nanocomposite solution onto the surface of a conductive substrate and then physically drying it for 20-60 minutes. The conductive substrate includes nickel foam, nano-gold, nano-carbon, or platinum black. The physical drying includes an oven or a heating table.

4. The application of the sensor as described in claim 1 in detecting menthone concentration, characterized in that, The detection range for menthone concentration is 0.2-5 mM.

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