Production method and system for producing high-quality enzymatic gelatin

Abstract

The invention provides a production method and system for producing high-quality enzymic gelatin, which can reduce the use of strong acid and strong alkali, and save equipment cost, production raw material cost and sewage treatment cost on the basis of ensuring the output of gelatin, the production method comprises the following steps: adding powder to be treated into an acetic acid solution with the pH value of 2.5, fully mixing and stirring for 30 minutes, filtering, washing, and drying to obtain the high-quality enzymic gelatin. Adding 10000U / g of pepsin powder, performing enzymolysis for 1.5-2 hours, performing centrifugation, performing dialysis, performing secondary centrifugation, and performing drying so as to obtain first enzymolysis powder; adding a sodium hydroxide solution with the pH value of 8.5 into the first enzymolysis powder, stirring in a water bath at 45 DEG C for 30 minutes, adding Elka enzyme, performing constant-temperature enzymolysis in the water bath at 50 DEG C for 3-3.5 hours, centrifuging, dialyzing, performing secondary centrifugation, and drying to obtain second enzymolysis powder; adding deionized water into the second enzymolysis powder, and heating in a water bath to obtain an enzymatic gelatin solution; carrying out desalting treatment on the enzymatic gelatin solution; removing residual small molecular impurities and moisture in the photoresist solution through a reverse osmosis membrane; and evaporating and concentrating to obtain the enzymatic gelatin.

Description

Technical Field

[0001] The invention relates to the field of enzymatic gelatin production, and in particular to a production method and system for producing high-quality enzymatic gelatin. Background Art

[0002] The traditional enzymatic gelatin production process is as follows: 1. Raw material preparation, raw material types: various animal skins, bones and other raw materials can be used. For example, the skins of animals such as cattle and pigs can be used as raw materials for the production of gelatin. First, chop or powder the various raw materials to prepare for subsequent enzymatic hydrolysis. 2. Enzymatic hydrolysis process, enzymatic hydrolysis conditions: under the conditions of temperature of 40-45℃ and pH of 5.0-6.0, add an appropriate amount of rare earth enzyme protease for enzymatic hydrolysis. The purpose of enzymatic hydrolysis is to remove impurity proteins in the raw materials. Then acetic acid dissolution: the raw materials after enzymatic hydrolysis are dissolved with acetic acid, salt precipitation of collagen fibers: use salt to precipitate collagen fibers, and then dehydrate and defatted the collagen fibers; boil gelatin: add the treated collagen fibers to distilled water and boil gelatin at 60-70℃; concentrate and dry: finally, concentrate and dry the boiled gelatin to obtain the enzymatic gelatin product.

[0003] The advantages of enzymatic production are: short production cycle: the production cycle is shortened from 1-3 months of the alkaline method to about 10 days; high gelatin yield: the gelatin yield is increased from about 50% to nearly 100%. However, the traditional enzymatic production time is still relatively long. Based on this, a method for quickly preparing bone gelatin with patent number 202310681721.2 is proposed, which uses strong acid and strong alkali combined with acidic enzyme and alkaline enzyme production process to shorten the production cycle and improve efficiency. However, with such a large amount of strong acid and strong alkali, the entire production system needs to be equipped with production equipment with higher safety factors and higher costs to provide technical support to prevent corrosion. The strong acid and strong alkali solution discharged by centrifugation after production is discharged to the sewage treatment equipment for treatment. There is a strong acid and strong alkali neutralization reaction, which requires a large amount of strong acid and strong alkali solution to be used in each production, resulting in an increase in production costs. At the same time, the pipelines and treatment equipment of the sewage treatment equipment need to be significantly upgraded to meet the sewage discharge treatment intensity of the entire gelatin production process. Summary of the invention

[0004] The object of the present invention is to provide a production method and system for producing high-quality enzymatic gelatin, which can reduce the use of strong acids and alkalis, and save equipment costs, production raw material costs, and sewage treatment costs while ensuring gelatin output.

[0005] The embodiment of the present invention is achieved as follows:

[0006] A method for producing high-quality enzymatic gelatin, the method comprising:

[0007] The powder to be treated is added to an acetic acid solution with a pH of 2.5, and the mixture is fully mixed and stirred for 30 minutes to perform a preliminary demineralization reaction, and then 10000U / g pepsin powder is added for enzymatic hydrolysis for 1.5-2 hours to precipitate the crude gelatin in the bone powder, and the enzyme is inactivated at 85-90°C for 5 minutes, and the powder is centrifuged, dialyzed, centrifuged again, and dried to obtain the first enzymatic hydrolysis powder;

[0008] Adding a sodium hydroxide solution with a pH of 8.5 to the first enzymatic hydrolysis powder, stirring in a water bath at 45° C. for 30 minutes, adding Alka enzyme and performing constant temperature enzymolysis in a water bath at 50° C. for 3-3.5 hours, using Alka enzyme to hydrolyze the first enzymatic hydrolysis powder to obtain osteoin hydrolyzed collagen, which exhibits a good ability to scavenge hydroxyl free radicals, inactivating the enzyme at 85-90° C. for 5 minutes, centrifuging, dialysis, secondary centrifuging, and drying to obtain a second enzymatic hydrolysis powder;

[0009] Deionized water is added to the second enzymatic hydrolysis powder, and the solution is heated in a water bath to obtain an enzymatic gelatin solution; the enzymatic gelatin solution is desalted; small molecular impurities and water remaining in the gelatin solution are removed by a reverse osmosis membrane; and the enzymatic gelatin is obtained by evaporation and concentration.

[0010] In a preferred embodiment of the present invention, the centrifugation, dialysis and secondary centrifugation steps in S1 and S2 are:

[0011] The enzymatic hydrolysis solution is centrifuged to remove the supernatant acid / alkali solution, the supernatant acid complex solution is introduced into a primary strong acetic acid reaction tank, and the supernatant alkali complex solution is introduced into a primary sodium hydroxide reaction tank to obtain osteoin precipitate;

[0012] Deionized water was used to wash the osteoin precipitate at a solid-liquid ratio of 1:3 (w / v);

[0013] Centrifuge twice at 4800 r / min for 15 min to remove the supernatant acid / alkali solution.

[0014] In a preferred embodiment of the present invention, the evaporation concentration is carried out at a vacuum degree of 0.1 MPa and a concentration temperature of 55°C.

[0015] In a preferred embodiment of the present invention, the stirring temperature of the acetic acid solution and the powder to be treated is room temperature 20-25°C; the enzymatic hydrolysis temperature of pepsin is 30-32°C.

[0016] In a preferred embodiment of the present invention, the powder to be processed includes bone meal or second enzymatic hydrolysis powder after one treatment.

[0017] In a preferred embodiment of the present invention, the above-mentioned method for desalting the enzymatic gelatin solution is to use 2% 732 cationic resin and 2% 717 anionic resin for desalting at 30-35°C for 20 minutes.

[0018] In a preferred embodiment of the present invention, the reverse osmosis membrane removes small molecular impurities and water remaining in the glue solution until the glue solution concentration reaches 15%.

[0019] In a preferred embodiment of the present invention, the above-mentioned dialysis, secondary centrifugation and drying steps are performed 1-2 times.

[0020] A production system for producing high-quality enzymatic gelatin, comprising a primary strong acetic acid reaction tank, a first centrifugation-dialysis-secondary centrifugation-drying treatment device, a primary sodium hydroxide reaction tank, a second centrifugation-dialysis-secondary centrifugation-drying treatment device, a secondary strong acetic acid reaction tank, a pepsin powder enzymolysis reaction tank, a secondary weak alkaline sodium hydroxide solution reaction tank, and an alcase enzymolysis reaction tank, wherein the primary strong acetic acid reaction tank, the first centrifugation-dialysis-secondary centrifugation-drying treatment device, the primary sodium hydroxide reaction tank, and the second centrifugation-dialysis-secondary centrifugation-drying treatment device are connected in sequence, and the secondary strong acetic acid reaction tank, the pepsin powder enzymolysis reaction tank, the first centrifugation-dialysis-secondary centrifugation-drying treatment device, the secondary weak alkaline sodium hydroxide solution reaction tank, the alcase enzymolysis reaction tank, and the second centrifugation-dialysis-secondary centrifugation-drying treatment device are connected in sequence;

[0021] The output end of the solution treated by the first centrifuge in the first centrifugation-dialysis-secondary centrifugation-drying treatment device is connected to the input end of the primary strong acetic acid reaction tank;

[0022] The output end of the solution treated by the first centrifuge in the second centrifugation-dialysis-secondary centrifugation-drying treatment device is connected to the input end of the primary sodium hydroxide reaction tank.

[0023] In a preferred embodiment of the present invention, the first centrifugation-dialysis-secondary centrifugation-drying treatment device and the second centrifugation-dialysis-secondary centrifugation-drying treatment device are combined devices consisting of a centrifuge, a dialysis device, a centrifuge, and a drying device.

[0024] The beneficial effects of the embodiments of the present invention are:

[0025] 1. Improved gelatin precipitation efficiency: This technical solution adopts a processing technology of strong acid compound pepsin + weak base compound alkaline enzyme + deionized water neutralization. Compared with the traditional gelatin production process of strong acid and strong base combined with acidic enzyme and alkaline enzyme, this production process control can still complete the processing within 14 hours even if two extraction treatments are completed, and the processing efficiency is extremely high;

[0026] 2. Compared with the strong acid + strong alkali processing technology, this technical solution adopts a strong acid and weak alkali environment, reducing the use of strong alkali, which has advantages in terms of equipment safety, equipment selection cost, sewage treatment, personnel protection, etc. For example, it can reduce the workload of sewage treatment equipment, thereby reducing the construction and operation investment of sewage treatment equipment;

[0027] 3. In gelatin production, the mixed solution of acetic acid and pepsin powder after the first centrifugation and the mixed solution of sodium hydroxide and alcase after centrifugation can be used for the first pre-extraction of bone powder. The operation steps are similar to the last one, but the reaction time is halved; this can reduce cost waste and improve the gelatin yield.

[0028] 4. Deionized water is used in the dialysis process to dialyze to neutrality to avoid the waste of raw materials caused by the neutralization reaction of strong acids and strong bases, so that the used strong acid and weak base solutions such as acetic acid and sodium hydroxide can be reused in the pretreatment stage. The amount of powder after pretreatment has been greatly reduced by filtration, and less strong acetic acid and sodium hydroxide weak base solution is required for secondary treatment, thereby saving costs; at the same time, deionized water can be recycled through regeneration technology, thereby greatly reducing water consumption and reducing the use of sewage treatment equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0030] Figure 1 A flow chart of a production method for producing high-quality enzymatic gelatin according to an embodiment of the present invention;

[0031] Figure 2 This is a production system diagram for producing high-quality enzymatic gelatin according to an embodiment of the present invention. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0033] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0035] First embodiment

[0036] See also Figure 1 This embodiment provides a method for producing high-quality enzymatic gelatin, the method comprising:

[0037] S1: Add animal bone powder with a particle size of less than 0.6 mm into a primary strong acetic acid reaction tank containing a mixed solution of strong acetic acid and pepsin powder for pretreatment, fully mix and stir for 1-1.5 hours to perform a preliminary demineralization reaction and remove the fat in the bone powder. At the same time, acetic acid can remove the mineralized collagen fibers in the animal bone powder. After demineralization, the mineralized collagen fiber bundles in the bone are destroyed, and hydroxyapatite is dissolved, which can be directly used for the next enzymatic hydrolysis;

[0038] Then enter the first centrifugation-dialysis-secondary centrifugation-drying treatment device to obtain dry bone powder;

[0039] S2: Add the dried bone meal to a primary sodium hydroxide reaction tank containing a mixed solution of weak sodium hydroxide solution and Alka enzyme for pretreatment, fully mix and stir for 1-1.5 hours for preliminary pre-precipitation reaction, and the weak alkaline environment created can also react with the fat in the animal bone meal for saponification. The entire secondary enzymatic hydrolysis reaction process takes 1-1.5 hours, which is sufficient to remove the fat;

[0040] Then enter the second centrifugation-dialysis-secondary centrifugation-drying treatment device to obtain dry bone powder;

[0041] S3: Add bone powder to an acetic acid solution with a pH of 2.5, and stir thoroughly for 30 minutes for a preliminary demineralization reaction; in fact, the demineralization reaction of animal bone powder in an acetic acid solution with a lower pH value is faster, but the faster the demineralization reaction, the stronger the acid solution will destroy the gelatin result, affecting the gelatin yield and gel texture parameters in the later stage. The pH 2.5 selected in this embodiment can not only meet the faster reaction speed, but also in this acidic environment, the pepsin activity is in the best state, the swelling effect on bone powder is the best, and the efficiency of extracting crude gelatin from bone powder is the highest;

[0042] Then, 10000U / g of pepsin powder was added for enzymatic hydrolysis for 1.5-2h to precipitate crude gelatin from the bone meal. The gelatin content increased with the increase of enzyme addition. When the pepsin addition was 10000U / g, the gelatin extraction rate was the highest. As the enzyme addition increased again, the crude gelatin extraction rate began to decrease. The reason is: when the amount of pepsin added was too low, the bone meal was not fully hydrolyzed, resulting in a low extraction rate. When the amount of pepsin added was too high, excessive enzymatic hydrolysis of the bone meal also resulted in a decrease in the extraction rate. Therefore, when the amount of pepsin added during the extraction process was 10000U / g, the extraction rate was high and more appropriate. This value was selected as the condition for subsequent experiments.

[0043] Among them, the stirring temperature of the acetic acid solution and the animal bone meal is 20-25℃ at room temperature; the enzymatic hydrolysis temperature of pepsin is 30-32℃. The optimal enzymatic hydrolysis temperature of general pepsin is 37℃, but near this optimal temperature, due to the high enzymatic activity of pepsin, bone gelatin is over-enzymatically hydrolyzed, resulting in a decrease in the yield, viscosity and gel strength of bone gelatin. And when the temperature is higher than 32℃, the combined action of heat treatment and enzyme treatment will excessively damage the bone gelatin that has lost the protection of the hydroxyapatite crystal region in the osteoin, causing the demineralized bone gelatin to denature and degrade into small molecule peptides, and the gel strength and viscosity of bone gelatin are reduced. After the enzymatic hydrolysis is completed, a part of the small molecule short chains produced by the excessive enzymatic hydrolysis of bone gelatin are easily soluble in water and are lost during the cleaning process, resulting in a decrease in the yield of bone gelatin products. Taking into account the yield, gel strength and viscosity of bone gelatin, the optimal enzyme action temperature is selected to be 30-32℃.

[0044] The enzyme is inactivated at 85-90°C for 5 minutes, and the supernatant is removed by centrifugation. The supernatant stock solution or the solute content is adjusted to obtain the primary first enzymatic hydrolysis powder in the pretreatment step, and the centrifugal precipitate is retained for dialysis operation; the dialysis is performed, and the centrifugal precipitate is neutralized with deionized water; the second centrifugation is performed, and the supernatant is removed for standby use, and the centrifugal precipitate is retained for drying to obtain the first enzymatic hydrolysis powder;

[0045] S4: Add sodium hydroxide solution with a pH of 8.5 to the first enzymatic hydrolyzate powder, stir in a water bath at 45°C for 30 minutes, use weakly alkaline sodium hydroxide to carry out a preliminary reaction, remove the acidic substances in the first enzymatic hydrolyzate powder that have not been removed by centrifugation, dialysis, secondary centrifugation, and drying, and create a weakly alkaline environment suitable for Alka enzyme; compared with the traditional method for preparing gelatin under a strong acid + strong base environment, the strong acid + weak base extraction method in this technical solution greatly reduces the high-intensity sewage treatment load and equipment investment during the preparation process.

[0046] S5: Add Alka enzyme and perform enzymolysis in a 50℃ water bath for 3-3.5h. Alka enzyme can hydrolyze macromolecular proteins into products such as polypeptides and amino acids, and has a strong ability to decompose proteins, while shortening the gelatin extraction time; two weak alkaline sodium hydroxide solutions and Alka enzyme environment can further break the hydrogen bonds of bone gelatin, so that collagen completely unwinds the triple helix to form a single chain, and a large amount of bone gelatin products are formed during the later heating process;

[0047] Inactivate the enzyme at 85-90°C for 5 minutes, centrifuge, dialyze, centrifuge again, and dry to obtain the second enzymatic hydrolyzate powder;

[0048] S6: adding deionized water to the second enzymatic hydrolysis powder to neutralize the second enzymatic hydrolysis powder in a weak alkaline environment, and heating the precipitate in a water bath to obtain an enzymatic gelatin solution;

[0049] The enzymatic gelatin solution is further desalted and purified; the small molecule impurities and water remaining in the gelatin solution are removed by reverse osmosis membrane; high-quality enzymatic gelatin is obtained by evaporation and concentration. More specifically, the evaporation and concentration is carried out at a vacuum degree of 0.1 MPa and a concentration process of 55°C.

[0050] More specifically, the steps of centrifugation, dialysis, secondary centrifugation and drying are:

[0051] After the enzymatic hydrolysis solution is centrifuged, the supernatant acid / alkali complex solution is removed, the supernatant acid complex solution is introduced into a primary strong acetic acid reaction tank, and the supernatant alkali complex solution is introduced into a primary sodium hydroxide reaction tank to obtain osteoin precipitate;

[0052] Deionized water is used to wash the osteoin precipitate at a material-liquid ratio of 1:3 (w / v). The ionized water after washing and neutralization enters the mixed bed resin regeneration device of the sewage treatment equipment, and deionized water that can be used again is obtained after backwashing and stratification, water discharge, regeneration liquid feeding, regeneration replacement, mixing of cation and anion resins, and forward washing.

[0053] Secondary centrifugation at 4800r / min for 15min to remove the supernatant acid / alkali solution. The amount of supernatant acid / alkali solution after secondary centrifugation is small and the pH value is more appropriate, so it is directly discharged to the sewage treatment equipment for treatment;

[0054] Drying: conventional drying equipment is used for drying, and the water generated by drying is directly discharged to the sewage treatment equipment for treatment.

[0055] More specifically, the enzymatic gelatin solution desalination method is to use 2% 732 cationic resin and 2% 717 anionic resin for 20 minutes at 30-35°C. The reverse osmosis membrane removes the small molecular impurities and water remaining in the gelatin solution until the gelatin solution concentration reaches 15%.

[0056] The centrifugation, dialysis, secondary centrifugation and drying steps in the secondary reaction process can be performed one more time to improve the purity of gelatin.

[0057] In summary, the advantages of the improved gelatin production process are:

[0058] 1. Improved gelatin precipitation efficiency: This technical solution adopts a processing technology of strong acid compound pepsin + weak base compound alkaline enzyme + deionized water neutralization. Compared with the traditional acid method, alkaline method or enzyme method gelatin production process, this production process control can still complete the processing within 14 hours even if two extraction treatments are completed, and the processing efficiency is extremely high;

[0059] At the same time, compared with the strong acid + strong alkali processing technology, this technical solution adopts a strong acid and weak alkali environment, reducing the use of strong alkali, which has benefits in terms of equipment safety, equipment selection cost, sewage treatment, personnel protection, etc. For example, it can reduce the workload of sewage treatment equipment, thereby reducing the construction and operation investment of sewage treatment equipment;

[0060] 2. In gelatin production, the mixed solution of acetic acid and pepsin powder after the first centrifugation and the mixed solution of sodium hydroxide and alcase after centrifugation can be used for the first pre-extraction of bone powder. The operation steps are similar to the last one, but the reaction time is halved; this can reduce cost waste and improve the gelatin yield.

[0061] 3. Deionized water is used in the dialysis process to dialyze to neutrality, reducing the occurrence of neutralization reactions such as strong acids and strong bases, so that used strong acids such as acetic acid and sodium hydroxide can be reused in the pretreatment stage by adjusting the solute content. At the same time, deionized water can be recycled through regeneration technology, thereby greatly reducing water consumption and reducing the use of sewage treatment equipment.

[0062] Second embodiment

[0063] See also Figure 2 This embodiment provides a production system for producing high-quality enzymatic gelatin, which is designed based on the production method of producing high-quality enzymatic gelatin, and includes: a primary strong acetic acid reaction tank, a first centrifugation-dialysis-secondary centrifugation-drying treatment device, a primary sodium hydroxide reaction tank, a second centrifugation-dialysis-secondary centrifugation-drying treatment device, a secondary strong acetic acid reaction tank, a pepsin powder enzymatic reaction tank, a secondary weak alkaline sodium hydroxide solution reaction tank, and an Alka enzyme enzymatic reaction tank. Among them,

[0064] The primary strong acetic acid reaction tank is connected to the first centrifuge in the first centrifugation-dialysis-secondary centrifugation-drying treatment device, and the strong acetic acid pepsin mixed solution centrifuged in the primary treatment and secondary treatment processes is refluxed to the primary strong acetic acid reaction tank for pretreatment;

[0065] The first centrifugation-dialysis-secondary centrifugation-drying treatment device is used to specifically treat the osteoin material after being treated with a strong acid solution, and is composed of a centrifuge, a dialysis neutralizer, and a dryer; the drying treatment device is connected to a primary sodium hydroxide reaction tank or an alkaline sodium hydroxide solution reaction tank, and is used to perform an alkaline solution reaction in the primary treatment or secondary treatment process;

[0066] The output end of the primary sodium hydroxide reaction tank is connected to the first centrifuge input end of the second centrifuge-dialysis-secondary centrifuge-drying treatment device, and the first centrifuge solution is connected to the first centrifuge solution output end. The osteoin material after the first drying treatment enters the primary sodium hydroxide reaction tank, and the primary sodium hydroxide reaction tank is provided with a weak alkali / alkaline enzyme mixed solution in the secondary gelatin treatment process and after the centrifugation treatment.

[0067] The second centrifugation-dialysis-secondary centrifugation-drying treatment device is used to specifically treat the osteogenic substance after the weak alkaline solution treatment. It is composed of a centrifuge, a dialysis neutralizer and a dryer. The solution after dialysis, secondary centrifugation and drying treatment enters the sewage treatment device for treatment;

[0068] The output end of the secondary strong acetic acid reaction tank is connected to the input end of the pepsin enzymolysis reaction tank, an acetic acid solution with a pH of 2.5 is arranged in the secondary strong acetic acid reaction tank, and after being stirred evenly, it enters the pepsin enzymolysis reaction tank, and then pepsin powder with an activity of 10000U / g is added to the pepsin enzymolysis reaction tank for enzymolysis for 1.5-2h;

[0069] The output end of the pepsin enzymolysis reaction tank is connected to the first centrifuge of the first centrifugation-dialysis-secondary centrifugation-drying treatment device; the output end of the dryer is connected to the secondary weak alkaline sodium hydroxide solution reaction tank and the aika enzyme enzymolysis reaction tank in sequence, and the output end of the aika enzyme enzymolysis reaction tank is connected to the first centrifuge of the second centrifugation-dialysis-secondary centrifugation-drying treatment device, and high-quality gelatin is obtained after drying is completed.

[0070] This specification describes examples of embodiments of the present invention, and it is not intended that these embodiments illustrate and describe all possible forms of the present invention. It should be understood that the embodiments in the specification can be implemented in a variety of alternative forms. The drawings need not be drawn to scale; some features can be enlarged or reduced to show the details of specific components. The specific structural and functional details disclosed should not be interpreted as limiting, but are merely representative bases for teaching those skilled in the art to implement the present invention in various forms. It should be understood by those skilled in the art that the multiple features illustrated and described with reference to any of the figures can be combined with the features illustrated in one or more other figures to form embodiments that are not explicitly illustrated or described. The illustrated combined features provide representative embodiments for typical applications. However, various combinations and variations of features consistent with the teachings of the present invention can be used for specific applications or implementations as needed.

[0071] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for producing high-quality enzymatic gelatin, characterized in that: The production method comprises: adding the powder to be treated into an acetic acid solution with a pH of 2.5, fully mixing and stirring for 30 minutes, adding 10000U / g pepsin powder for enzymatic hydrolysis for 1.5-2 hours, inactivating the enzyme at 85-90°C for 5 minutes, centrifuging, dialysis, secondary centrifugation, and drying to obtain a first enzymatic hydrolysis powder; Add a sodium hydroxide solution with a pH of 8.5 to the first enzymatic hydrolysis powder, stir in a water bath at 45° C. for 30 min, add Alka enzyme, perform enzymolysis in a water bath at 50° C. for 3-3.5 h, inactivate the enzyme at 85-90° C. for 5 min, centrifuge, dialyze, centrifuge again, and dry to obtain a second enzymatic hydrolysis powder; Deionized water is added to the second enzymatic hydrolysis powder, and the solution is heated in a water bath to obtain an enzymatic gelatin solution; the enzymatic gelatin solution is desalted; small molecular impurities and water remaining in the gelatin solution are removed by a reverse osmosis membrane; and the enzymatic gelatin is obtained by evaporation and concentration.

2. The method for producing high-quality enzymatic gelatin according to claim 1, characterized in that: The centrifugation, dialysis and secondary centrifugation steps are: The enzymatic hydrolysis solution is centrifuged to remove the supernatant acid / alkali solution, the supernatant acid complex solution is introduced into a primary strong acetic acid reaction tank, and the supernatant alkali complex solution is introduced into a primary sodium hydroxide reaction tank to obtain an osteoin precipitate; the osteoin precipitate is washed with deionized water at a material-liquid ratio of 1:3 (w / v); Centrifuge twice at 4800 r / min for 15 min to remove the supernatant acid / alkali solution.

3. The method for producing high-quality enzymatic gelatin according to claim 1, characterized in that: The evaporation concentration was carried out at a vacuum degree of 0.1 MPa and a temperature of 55°C.

4. The method for producing high-quality enzymatic gelatin according to claim 1, characterized in that: The stirring temperature of the acetic acid solution and the powder to be treated is 20-25°C at room temperature; the enzymolysis temperature of pepsin is 30-32°C.

5. The method for producing high-quality enzymatic gelatin according to claim 1, characterized in that: The powder to be processed includes bone meal or second enzymatic hydrolysis powder after one treatment.

6. The method for producing high-quality enzymatic gelatin according to claim 1, characterized in that: The method for desalting the enzymatic gelatin solution is to use 2% 732 cationic resin and 2% 717 anionic resin for desalting at 30-35° C. for 20 minutes.

7. The method for producing high-quality enzymatic gelatin according to claim 1, characterized in that: The reverse osmosis membrane removes the small molecular impurities and water remaining in the glue solution until the concentration of the glue solution reaches 15%.

8. The method for producing high-quality enzymatic gelatin according to claim 1, characterized in that: The centrifugation, dialysis, secondary centrifugation and drying steps are performed 1-2 times.

9. A production system for producing high-quality enzymatic gelatin, characterized in that: It comprises a primary strong acetic acid reaction tank, a first centrifugation-dialysis-secondary centrifugation-drying treatment device, a primary sodium hydroxide reaction tank, a second centrifugation-dialysis-secondary centrifugation-drying treatment device, a secondary strong acetic acid reaction tank, a pepsin powder enzymolysis reaction tank, a secondary weak alkaline sodium hydroxide solution reaction tank, and an alka enzyme enzymolysis reaction tank. The primary strong acetic acid reaction tank, the first centrifugation-dialysis-secondary centrifugation-drying treatment device, the primary sodium hydroxide reaction tank, and the second centrifugation-dialysis-secondary centrifugation-drying treatment device are connected in sequence, and the secondary strong acetic acid reaction tank, the pepsin powder enzymolysis reaction tank, the first centrifugation-dialysis-secondary centrifugation-drying treatment device, the secondary weak alkaline sodium hydroxide solution reaction tank, the alka enzyme enzymolysis reaction tank, and the second centrifugation-dialysis-secondary centrifugation-drying treatment device are connected in sequence; The output end of the solution treated by the first centrifuge in the first centrifugation-dialysis-secondary centrifugation-drying treatment device is connected to the input end of the primary strong acetic acid reaction tank; The output end of the solution treated by the first centrifuge in the second centrifugation-dialysis-secondary centrifugation-drying treatment device is connected to the input end of the primary sodium hydroxide reaction tank.

10. The production system for producing high-quality enzymatic gelatin according to claim 9, characterized in that: The first centrifugation-dialysis-secondary centrifugation-drying treatment device and the second centrifugation-dialysis-secondary centrifugation-drying treatment device are combined devices consisting of a centrifuge, a dialysis device, a centrifuge, and a drying device.

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