Method for simultaneously determining low-content active chemical components in radix stephaniae tetrandrae and radix astragali decoction

Abstract

The invention provides a method for simultaneously determining low-content active chemical components in radix stephaniae tetrandrae and radix astragali decoction, and belongs to the technical field of traditional Chinese medicine component analysis, and the method comprises the following steps: decocting raw materials of the radix stephaniae tetrandrae and radix astragali decoction with water, and then performing ultrasonic extraction by using a methanol solution to obtain a test solution; and respectively and precisely sucking the mixed reference substance solution and the test solution by adopting a UHPLC-MS / MS method, and injecting the mixed reference substance solution and the test solution into an analytical instrument for determination. Compared with a traditional ultraviolet detector, mass spectrum can accurately determine the content of components and accurately give mass number information of parent ions and daughter ions of a to-be-detected object, and different from retention time, the mass number information serving as a basis for qualitative identification is more stable, reliable and high in specificity.

Images