Preparation method of neural stem cell homogenate and application of neural stem cell homogenate in repairing skin light injury and light aging
CN119530154APending Publication Date: 2025-02-28TAOKANG INST OF NEUROLOGY (XIAMEN) CO LTD
View PDF 0 Cites 0 Cited by
Patent Information
- Application Number
- CN202411751531.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-02
- Publication Date
- 2025-02-28
AI Technical Summary
Technical Problem
The prior art has shortcomings in preventing, repairing and treating photoaging, especially stem cell homogenates have been studied in this field.
Method used
By selecting fetal mice with gestational age of 11.5 to 15.5 days, primary neural stem cells were extracted from their cerebral cortex, cultured, and the growth factor concentration was adjusted by half a dose of liquid change, and finally neural stem cell homogenate was obtained through cell disruption treatment.
Benefits of technology
This method can efficiently obtain neural stem cell homogenate, broaden its application range, and significantly repair skin lesions in mouse photoaging models, improve wrinkle scores and skin elasticity, and have a dose effect.
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure SMS_1 
Figure SMS_2 
Figure SMS_3
Abstract
The invention relates to a preparation method and application of neural stem cell homogenate. The method comprises the following steps: extracting primary neural stem cells from cerebral cortex of fetal rats with the gestational age of 11.5-15.5 days; culturing the extracted primary neural stem cells, adjusting the concentration of growth factors for culture by adopting a half-volume liquid change mode, and culturing to P1 generation; and repeatedly freezing and thawing the P1-generation neural stem cells to obtain the neural stem cell homogenate. According to the method, the neural stem cell homogenate can be efficiently obtained, and the neural stem cell homogenate has a repairing effect on skin injury of a photoaged mouse constructed by medium-wave ultraviolet rays (UVB) and has a dose effect.
Need to check novelty before this filing date? Find Prior Art