一种新的高活性酪氨酸解氨酶及其应用
By optimizing the amino acid sequence and reaction conditions of tyrosine ammonia-lyase from *Cladosporium chrysogenum*, a highly efficient catalytic process for the production of p-coumaric acid from L-tyrosine in *Escherichia coli* was achieved. This solved the problem of insufficient catalytic activity of tyrosine ammonia-lyase and enabled the efficient production of p-coumaric acid, which has significant industrial application value.
CN119530209BActive Publication Date: 2026-07-17ZHEJIANG UNIV OF TECH
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ZHEJIANG UNIV OF TECH
- Filing Date
- 2024-11-01
- Publication Date
- 2026-07-17
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Figure CN119530209B_ABST
Abstract
本发明提供了一种新的高活性酪氨酸解氨酶及其应用，该酶来源于变色栓菌(Trametes versicolor)在大肠杆菌中进行重组表达，对目的基因进行密码子优化，优化后的DNA序列如SEQ ID NO.2所示。该酶可以高效催化酪氨酸高效合成对香豆酸，在对香豆酸生产领域有很大的经济价值。
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