A method for treating extraction wastewater from GA3 production

Abstract

The application discloses a GA3 production extraction wastewater treatment method, which comprises the following steps: S1, GA3 production extraction wastewater is loaded into a fermentation container, and after sterilization, a wine yeast seed liquid is inoculated, and the inoculation amount is 1-15 v / v%; S2, fermentation culture is carried out, and fermentation liquid is obtained, and the fermentation conditions are as follows: 25-35 DEG C, 50-600 r / min, and 24-200 h. The application can produce valuable single-cell protein while reducing COD, so that the purpose of treating wastewater is achieved.

Description

Technical Field

[0001] This invention relates to the field of wastewater treatment, and in particular to a method for treating GA3 production extraction wastewater. Background Technology

[0002] The production of GA3 (gibberellin) generates a large amount of extraction wastewater, which contains a large amount of nutrients such as polypeptides, amino acids, esters, alcohols, and trace elements, with a chemical oxygen demand (COD) as high as 50,000-100,000 mg / L.

[0003] The current practice is to directly send the extraction wastewater generated in GA3 production into the sewage treatment system for chemical, physical, and biochemical reactions, just like ordinary wastewater (the biochemical reactions of ordinary wastewater). This not only seriously increases the difficulty of subsequent sewage treatment, but also makes the GA3 production extraction wastewater extremely prone to putrefaction, deterioration, and foul odor, causing serious environmental pollution.

[0004] Therefore, how to effectively treat the GA3 production extraction wastewater before sending it into the wastewater treatment system has become an urgent problem for the industry.

[0005] The above background information is provided to facilitate understanding of the present invention and is not intended to be publicly known technology disclosed to the general public prior to the application of this invention. Summary of the Invention

[0006] To address the aforementioned shortcomings, this invention provides a method for treating GA3 production extraction wastewater. This method produces valuable single-cell proteins while reducing COD to achieve the purpose of wastewater treatment.

[0007] The technical solution is: a method for treating GA3 production extraction wastewater, comprising the following steps: S1, GA3 production extraction wastewater is loaded into a fermentation vessel, sterilized, and then inoculated with brewer's yeast seed culture at an inoculation rate of 1~15 v / v%. S2, fermentation culture, to obtain fermentation broth, fermentation conditions are: 25~35℃, 50~600r / min, 24~200 h.

[0008] Furthermore, the brewing yeast is Angel high-activity dry yeast.

[0009] Furthermore, in S2, the fermentation conditions are: 30~33℃, 200~300r / min, 96~120 h, pH 3.2~5.5; in S1, the inoculum size is 5~10 v / v.

[0010] Furthermore, 0.5~2g / L of potassium dihydrogen phosphate is added to S1.

[0011] Furthermore, in S1, the fermentation vessel is a shake flask.

[0012] Furthermore, S2 is followed by S3: concentrating and drying the fermentation broth from S2 to obtain a single-cell protein product. Furthermore, in S1, the fermentation container is a fermenter with a loading coefficient of 50-80%, and in S2, the actual digestion time is 20-50 minutes, with an aeration rate of 0.5-2 vvm.

[0013] Furthermore, S2 is followed by S3: the fermentation broth of S2 is concentrated and separated into a concentrated liquid and a dilute liquid through a membrane. The concentrated liquid is spray-dried to obtain a single-cell protein product, and the dilute liquid is wastewater, which is discharged into the sewage treatment system.

[0014] Furthermore, the brewer's yeast seed liquid is formed through the following steps: S01, YPD plate culture: After sterilization of YPD medium, the Saccharomyces cerevisiae strain was streaked on YPD plates for activation and cultured at 25~35℃ for 12~48h; S02, Shake Flask Culture: Pick a single colony from the YPD plate and inoculate it into a sterile YPD liquid shake flask. Incubate at 25~35℃ and 100~300r / min for 12~48h.

[0015] Furthermore, the brewer's yeast seed liquid is formed through the following steps: S01, YPD plate culture: After sterilization of YPD medium, the Saccharomyces cerevisiae strain was streaked on YPD plates for activation and cultured at 25~35℃ for 12~48h; S02, Shake flask culture: Pick a single colony from the YPD plate and inoculate it into a sterile YPD liquid shake flask. Incubate on a shaker at 25~35℃ and 100~300r / min for 12~48h. S03, Seed tank culture: Add the seed tank culture medium to a 15L seed tank with a filling coefficient of 50-80%, allow to digest for 20-50 minutes, inoculate at 1-5 v / v%, aeration at 100-300 r / min and 0.5-1 vvm, and culture at 25-35℃ for 12-48 hours.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention utilizes GA3 production extraction wastewater as a raw material to produce single-cell protein. Single-cell protein (SCP), also known as microbial protein or bacterial protein, is characterized by high protein content, balanced amino acid composition, high nutritional value, and safety. It is extremely rich in nutrients, with a protein content as high as 40-80%, and contains various vitamins, lipids, amino acids, trace elements, enzymes, and bioactive substances, making it suitable as food, feed, or food additives. This invention not only utilizes the organic matter in wastewater to produce single-cell protein but also reduces the organic matter content in wastewater, thereby achieving the treatment and purification of extraction wastewater. Detailed Implementation

[0017] As used in this article: "Prepared from" is synonymous with "comprising". The terms "comprising", "including", "having", "containing", or any other variations thereof as used herein are intended to cover non-exclusive inclusion. For example, a composition, step, method, article, or apparatus that includes the listed elements is not necessarily limited to those elements, but may include other elements not expressly listed or elements inherent to such composition, step, method, article, or apparatus.

[0018] The conjunction "composed of..." excludes any unspecified elements, steps, or components. If used in a claim, this phrase makes the claim closed, excluding materials other than those described, except for associated conventional impurities. When the phrase "composed of..." appears in a clause of the body of a claim rather than immediately following it, it limits only the elements described in that clause; other elements are not excluded from the claim as a whole.

[0019] When a quantity, concentration, or other value or parameter is expressed as a range, a preferred range, or a range defined by a series of upper and lower preferred values, this should be understood as specifically disclosing all ranges formed by any pair of any upper or preferred value with any lower or preferred value, regardless of whether the range is disclosed individually. For example, when the range “1–5” is disclosed, the described range should be interpreted as including ranges “1–4”, “1–3”, “1–2”, “1–2 and 4–5”, “1–3 and 5”, etc. When numerical ranges are described herein, unless otherwise stated, the range is intended to include its endpoints and all integers and fractions within that range.

[0020] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] Those skilled in the art will understand that the following embodiments are for illustrative purposes only and should not be construed as limiting the scope of this application. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents, instruments, or microorganisms used, unless otherwise specified by the manufacturer, are all commercially available conventional products.

[0022] In these embodiments, unless otherwise specified, the portions and percentages are all by weight.

[0023] "Parts by mass" refers to the basic unit of measurement that expresses the mass ratio of multiple components. One part can represent any unit mass, such as 1g or 2.689g. If we say that component A has "a" parts by mass and component B has "b" parts by mass, it means the ratio of the mass of component A to the mass of component B is a:b. Alternatively, it can mean that the mass of component A is aK and the mass of component B is bK (K is any number representing a multiplier). It is important to understand that, unlike the number of parts by mass, the sum of the mass parts of all components is not limited to 100 parts.

[0024] "And / or" is used to indicate that one or both of the described situations may occur, for example, A and / or B includes (A and B) and (A or B).

[0025] In this invention, OD is the optical density value of the fermentation broth at a wavelength of 570 nm, measured using a UV-Vis spectrophotometer.

[0026] In the following examples and comparative examples, the shake flask culture medium consisted of 1% yeast extract, 2% peptone, 2% glucose, and the remainder was water; the seed tank culture medium consisted of 1 wt% yeast extract, 2 wt% peptone, 2 wt% glucose, 0.05 wt% bubbling agent, and the remainder was water.

[0027] In the following examples and comparative examples, the initial COD of the extraction wastewater was 73468 mg / L.

[0028] In the following examples, the brewing yeast was Angel Yeast High Activity Dry Yeast - Low Sugar Type 500g (Angel Yeast Co., Ltd.).

[0029] Example 1: Preparation of Saccharomyces cerevisiae seed culture, Yersinia lipolytica seed culture, and Candida albicans seed culture S01, YPD plate culture: After sterilization of YPD medium, Saccharomyces cerevisiae, Yersinia lipolytica and Candida albicans strains were streaked on YPD plates for activation and cultured at 30℃ for 24h. S02, Shake flask culture: Pick a single colony from the YPD plate and inoculate it into a sterile YPD liquid shake flask. Incubate at 30℃ and 200r / min for 24h on a shaker. Saccharomyces cerevisiae seed liquid, Yersinia lipolytica seed liquid, and Candida albicans seed liquid were obtained respectively.

[0030] Example 2 A method for treating GA3 production extraction wastewater includes the following steps: S1, GA3 production extraction wastewater is put into a fermentation shake flask, sterilized, and then inoculated with the brewing yeast seed liquid of Example 1 at an inoculation amount of 10 v / v.

[0031] S2 was cultured at 30℃ and 200r / min for 114h with natural pH (3.2-3.5) to obtain fermentation broth. The OD and COD contents in the fermentation broth were detected, and the results are shown in Table 1.

[0032] S3, the fermentation broth was concentrated and dried to obtain single-cell protein powder. The amount of single-cell protein is shown in Table 1, and the crude protein content is shown in Table 1.

[0033] Example 3 The only difference between this embodiment and embodiment 2 is that in this embodiment, the culture temperature in S2 is 33°C.

[0034] The OD and COD contents in the fermentation broth were detected, and the results are shown in Table 1. The yield of single-cell protein and the crude protein content are shown in Table 1.

[0035] Example 4 The only difference between this embodiment and embodiment 2 is that in this embodiment, the pH in S2 is 5.5.

[0036] The OD and COD contents in the fermentation broth were detected, and the results are shown in Table 1. The yield of single-cell protein and the crude protein content are shown in Table 1.

[0037] Comparative Example 1 The only difference between this comparative example and Example 2 is that, in this comparative example, in S1, the inoculated material is the Yeast lipolytica seed culture of Example 1.

[0038] The OD and COD contents in the fermentation broth were detected, and the results are shown in Table 1. The yield of single-cell protein and the crude protein content are shown in Table 1.

[0039] Comparative Example 2 The only difference between this comparative example and comparative example 1 is that the culture temperature in S2 of this comparative example is 33℃.

[0040] The OD and COD contents in the fermentation broth were detected, and the results are shown in Table 1. The yield of single-cell protein and the crude protein content are shown in Table 1.

[0041] Comparative Example 3 The only difference between this comparative example and comparative example 1 is that in this comparative example, the pH in S2 is 5.5.

[0042] The OD and COD contents in the fermentation broth were detected, and the results are shown in Table 1. The yield of single-cell protein and the crude protein content are shown in Table 1.

[0043] Comparative Example 4 The only difference between this comparative example and Example 2 is that in this comparative example, in S1, the inoculated product is the Candida seed culture of Example 1.

[0044] The OD and COD contents in the fermentation broth were detected, and the results are shown in Table 1. The yield of single-cell protein and the crude protein content are shown in Table 1.

[0045] Comparative Example 5 The only difference between this comparative example and comparative example 4 is that the culture temperature in S2 of this comparative example is 33℃.

[0046] The OD and COD contents in the fermentation broth were detected, and the results are shown in Table 1. The yield of single-cell protein and the crude protein content are shown in Table 1.

[0047] Comparative Example 6 The only difference between this comparative example and comparative example 4 is that in this comparative example, the pH in S2 is 5.5.

[0048] The OD and COD contents in the fermentation broth were detected, and the results are shown in Table 1. The yield of single-cell protein and the crude protein content are shown in Table 1.

[0049] Example 5 S01, YPD plate culture: After sterilization of YPD medium, the Saccharomyces cerevisiae strain was streaked on YPD plates for activation and cultured at 30℃ for 24h. S02, Shake flask culture: Pick a single colony from the YPD plate and inoculate it into a sterile YPD liquid shake flask. Incubate at 30℃ and 200r / min for 24h on a shaker. S03, Seed tank culture: Add the seed tank culture medium to a 15L seed tank with a filling coefficient of 70%, and let it sterilize for 30 minutes. Inoculate the S02 seed tank in a shake flask with an inoculation amount of 2v / v%, an aeration rate of 0.7vvm at 200r / min, and culture at 30℃ for 24h.

[0050] Obtain the brewer's yeast seed liquid.

[0051] Example 6 A method for treating GA3 production extraction wastewater includes the following steps: S1. The GA3 production extraction wastewater is loaded into a fermenter (50L), sterilized, and then inoculated with the brewing yeast seed liquid of Example 5, with a loading coefficient of 70% and an inoculation amount of 8-10%.

[0052] S2, actual sterilization for 30 min, aeration rate of 0.7 vvm at 300 r / min, defoaming agent automatically controlled, pH naturally not adjusted, cultured at 30℃ for 114 h to obtain fermentation broth.

[0053] S3, the fermentation broth was concentrated using a membrane to separate it into a concentrated liquid and a dilute liquid. The concentrated liquid was spray-dried to obtain a single-cell protein product (results are shown in Table 1, with crude protein content shown in Table 1). The dilute liquid was wastewater, which was discharged into the wastewater treatment system. The COD of the discharged wastewater was 21239 mg / L. The OD and COD contents in the fermentation broth were detected, and the results are shown in Table 1.

[0054] Example 7 The difference between this embodiment and Embodiment 6 is that in S1, 1 g / L of potassium dihydrogen phosphate (calculated at a loading coefficient of 70%, the amount added is 35 g) is also added to the fermenter. The COD of the discharged wastewater is 18205 mg / L. The OD and COD contents in the fermentation broth were detected, and the results are shown in Table 1. The yield of single-cell protein and the crude protein content are shown in Table 1.

[0055] Table 1 .

[0056] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for treating GA3 production extraction wastewater, characterized in that, Includes the following steps: S1, GA3 production extraction wastewater is loaded into a fermentation vessel, sterilized, and then inoculated with the selected Saccharomyces cerevisiae seed liquid at an inoculation amount of 1~15 v / v%; the screening includes: comparing the COD removal rates of Saccharomyces cerevisiae, Yersinia lipolytica and Candida albicans in GA3 production extraction wastewater under the same fermentation conditions, and selecting the Saccharomyces cerevisiae with the highest COD removal rate. S2, fermentation culture to obtain fermentation broth, fermentation conditions are: 25~35℃, 50~600r / min, 24~200 h, pH 3.2~3.5; S3, the fermentation broth of S2 is concentrated and separated into concentrated liquid and dilute liquid through membrane. The concentrated liquid is spray dried to obtain single-cell protein product, and the dilute liquid is wastewater, which is discharged into the sewage treatment system. In S1, when the fermentation vessel is a fermenter, 0.5~2g / L of potassium dihydrogen phosphate is also added.

2. The method for treating GA3 production extraction wastewater according to claim 1, characterized in that, The brewing yeast is Angel high-activity dry yeast.

3. The method for treating GA3 production extraction wastewater according to claim 2, characterized in that, In S2, the fermentation conditions are: 30~33℃, 200~300r / min, 96~120 h; in S1, the inoculum size is 5~10 v / v.

4. The method for treating GA3 production extraction wastewater according to claim 1, characterized in that, In S1, the loading coefficient is 50-80%; in S2, the actual consumption is 20-50 minutes, and the ventilation rate is 0.5-2 vvm.

5. The method for treating GA3 production extraction wastewater according to any one of claims 1-4, characterized in that, The brewer's yeast seed liquid is formed through the following steps: S01, YPD plate culture: After sterilization of YPD medium, the Saccharomyces cerevisiae strain was streaked on YPD plates for activation and cultured at 25~35℃ for 12~48h; S02, Shake flask culture: Pick a single colony from the YPD plate and inoculate it into a sterile YPD liquid shake flask. Incubate on a shaker at 25~35℃ and 100~300r / min for 12~48h. S03, Seed tank culture: Add the seed tank culture medium to a 15L seed tank with a filling coefficient of 50-80%, allow to digest for 20-50 minutes, inoculate at 1-5 v / v%, aeration at 100-300 r / min and 0.5-1 vvm, and culture at 25-35℃ for 12-48 hours.