一种基于脱氧核酶和光子晶体技术的微流控芯片及其应用
CN119592410BActive Publication Date: 2026-07-17DALIAN UNIV OF TECH
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- DALIAN UNIV OF TECH
- Filing Date
- 2024-11-22
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing technologies are insufficient for rapid, sensitive, and highly selective detection of E. coli, especially in field applications where they are complex to operate and expensive.
Method used
A microfluidic chip based on deoxyribozyme and photonic crystal technology was designed, comprising a deoxyribozyme inlet, a serpentine reaction channel, a magnetic bead enrichment chamber, and a signal detection chamber. The fluorescence signal is enhanced in the photonic crystal signal detection chamber after the deoxyribozyme reacts with the E. coli sample.
Benefits of technology
It achieves rapid response and high sensitivity detection, with a detection limit of 103 CFU/mL within 20 minutes. It has significant specificity and low cost, making it easy to promote and apply.
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Figure CN119592410B_ABST
Abstract
本发明公开一种基于脱氧核酶和光子晶体技术的微流控芯片及其应用，属于分析检测技术领域。本发明基于脱氧核酶和光子晶体技术的微流控芯片主要由脱氧核酶进样口，大肠杆菌样品进样口，蛇形反应流道，磁珠富集腔室和信号检测腔室组成，聚苯乙烯球作为光子晶体组装在信号检测腔室，磁铁固定在磁珠富集腔室底部。在反应流道中，偶联在磁珠上的脱氧核酶与大肠杆菌靶标发生反应后，带有FAM荧光基团的底物链断裂，随着流体的流动进入到信号检测腔室，在488nm激发光照射下，使用荧光显微镜观察荧光图像，荧光强弱与大肠杆菌靶标的含量成正比，检测时间需要约25min。本发明的微流控芯片轻巧便携、检测迅速、灵敏度高、选择性好，可实现对待测物的现场定量检测。
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