Wheat sts marker related to lateral root length and drought resistance and application thereof

Abstract

The application belongs to the technical field of biology, and discloses a wheat lateral root length and drought resistance related STS marker and application thereof. The application provides application of an InDel molecular marker, wherein the DNA fragment of the InDel molecular marker is the nucleotide sequence of 84th-103rd of SEQ ID NO:1, the application is identification or auxiliary identification of wheat lateral root length and / or drought resistance, and the application can be used for wheat breeding. The STS molecular marker can provide a strong selection site, improve the accuracy of selection of breeding materials of the lateral root length and drought resistance related traits, and realizes the goal of molecular marker assisted selection of excellent wheat varieties.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, and more specifically, to STS markers related to wheat lateral root length and drought resistance, and their applications. Background Technology

[0002] The root system is the core organ for wheat to absorb soil moisture and is also the first organ to sense soil drought. Wheat roots include seed roots and adventitious roots, but all branches produced by any type of root are collectively called lateral roots. Lateral roots provide additional support to the plant, expand its ability to absorb soil water, increase soil compaction and water conservation, improve soil permeability and oxygen supply, and enhance drought adaptability. They are crucial tissues that are expected to be improved in drought-resistant breeding. However, lateral roots are dense and deeply embedded in the soil, making direct selection through visual observation difficult. The rapid development of molecular marker technology has provided a new opportunity for efficient selection of lateral roots. Therefore, discovering and utilizing molecular markers related to lateral root traits, and accelerating lateral root improvement through marker-assisted selection, is an urgent need for the sustainable development of wheat production. Summary of the Invention

[0003] The technical problem to be solved by this invention is how to quickly identify the lateral root length and drought resistance of wheat.

[0004] To address the aforementioned problems, this invention first provides an application of the InDel molecular marker, wherein the InDel molecular marker is a DNA fragment with nucleotide sequence SEQ ID No: 1, positions 84-103, and the application is the use of the InDel molecular marker in any of the following:

[0005] A1) Identification or auxiliary identification of wheat lateral root length;

[0006] A2) Prepare products for identification or auxiliary identification of wheat lateral root length;

[0007] A3) Identify or assist in the identification of wheat drought resistance;

[0008] A4) Prepare products for identifying or assisting in the identification of wheat drought resistance;

[0009] A5) Wheat breeding;

[0010] A6) Prepare products for wheat breeding.

[0011] This invention also provides an application of a substance for detecting InDel molecular markers, wherein the InDel molecular marker is a DNA fragment with nucleotide sequence SEQ ID No: 1, positions 84-103, and the application is the use of the substance in any of the following:

[0012] B1) Identification or auxiliary identification of wheat lateral root length;

[0013] B2) Prepare products for identification or auxiliary identification of wheat lateral root length;

[0014] B3) Identification or auxiliary identification of wheat drought resistance;

[0015] B4) Prepare products for identifying or assisting in the identification of wheat drought resistance;

[0016] B5) Wheat breeding;

[0017] B6) Prepare products for wheat breeding.

[0018] The indicators for wheat breeding mentioned above may include wheat lateral root length and wheat drought resistance.

[0019] In the above text, the wheat lateral root length can be the total lateral root length of wheat; the wheat drought resistance can be the wheat drought resistance index.

[0020] Furthermore, in the aforementioned applications, the purpose of wheat breeding may include breeding wheat with altered total lateral root length (total lateral root length greater than the parent or total lateral root length less than the parent); it may also include breeding wheat with a drought resistance index superior to that of the parent.

[0021] In this application, "parent" refers to two different varieties or strains of plants used for hybridization, such as wheat.

[0022] In the above applications, the substance contains PCR primers for amplifying wheat genomic DNA fragments including the InDel molecular marker.

[0023] The PCR primers are primer pairs, which consist of a forward primer and a reverse primer. The forward primer is a single-stranded DNA that specifically binds to the upstream of position 84 of the double-stranded DNA shown in SEQ ID No: 1 of wheat genomic DNA, and the reverse primer is a single-stranded DNA that specifically binds to the downstream of position 103 of the double-stranded DNA shown in SEQ ID No: 1 of wheat genomic DNA.

[0024] The forward primer may be a single-stranded DNA with the nucleotide sequence SEQ ID No: 2, and the reverse primer may be a single-stranded DNA with the nucleotide sequence SEQ ID No: 3.

[0025] In the above applications, the substance can be a substance that detects the InDel molecular marker using at least one of the following methods: DNA sequencing, restriction enzyme fragment length polymorphism, single-strand conformation polymorphism, denaturing high-performance liquid chromatography, and SNP microarray. The SNP microarray includes microarrays based on nucleic acid hybridization reactions, microarrays based on single-base extension reactions, microarrays based on allele-specific primer extension reactions, microarrays based on one-step reactions, microarrays based on primer ligation reactions, microarrays based on restriction endonuclease reactions, microarrays based on protein-DNA binding reactions, and microarrays based on fluorescent molecule-DNA binding reactions.

[0026] In the above applications, the PCR primers may or may not be labeled with a marker. The marker refers to any atom or molecule that can be used to provide a detectable effect and can be linked to a nucleic acid. Markers include, but are not limited to, dyes; radioactive labels, such as 32P; binding moieties, such as biotin; haptens, such as digoxigenin (DIG); luminescent, phosphorescent, or fluorescent moieties; and fluorescent dyes alone or in combination with moieties whose emission spectra can be inhibited or shifted by fluorescence resonance energy transfer (FRET). The marker can provide a signal detectable by fluorescence, radioactivity, colorimetry, gravimetric determination, X-ray diffraction or absorption, magnetism, enzyme activity, etc. The marker can be a charged moiety (positive or negative charge) or, optionally, charge-neutral. The marker can include nucleic acid or protein sequences or combinations thereof, provided that the sequence containing the marker is detectable. In some embodiments, nucleic acids are detected directly without a marker (e.g., direct sequence reading).

[0027] The present invention also provides a method for identifying wheat lateral root length and / or drought resistance, comprising using the genomic DNA of the wheat to be identified as a template, performing PCR amplification with the above-mentioned specific primers to obtain PCR products, and identifying wheat lateral root length and / or drought resistance based on whether the PCR products contain the InDel molecular marker.

[0028] The present invention also provides a method for assisting in the identification of wheat lateral root length and / or drought resistance, comprising using the genomic DNA of the wheat to be identified as a template, performing PCR amplification with the above-mentioned specific primers to obtain PCR products, and identifying wheat lateral root length and / or drought resistance based on whether the PCR products contain the InDel molecular marker.

[0029] In the above method, the identification of wheat lateral root length and / or drought resistance based on whether the PCR product contains the InDel molecular marker can specifically be that the lateral root length and / or drought resistance of the wheat to be identified containing the InDel molecular marker is higher than that of the wheat to be identified without the InDel molecular marker.

[0030] In the above method, the wheat can be a wheat inbred line or a pure line, and the wheat can also be a hybrid offspring obtained by crossing at least two of the following wheat varieties (such as F2 generation or higher, such as BC1F1 or higher): Fuzhuang 30, Han 6172, Handan 6050, Heng 216, Heng 4399, Heng 5229, Luohan 2, Luohan 6, Luohan 7, Shanhe 6, Bainong 160, Boai 7023, Hengmai 2, Luomai 9, Luoyou 7, Yumai 29, Yumai 2, Yumai 38, Yumai 48, and Lumai 14.

[0031] The wheat variety can specifically be a hybrid offspring of Lumai 14 × Shanhe 6, such as BC1F1, BC2F1, BC3F1, BC3F2, BC3F3, BC3F4, or BC3F5. In the hybrid combination of Lumai 14 × Shanhe 6, Lumai 14 is the female parent and Shanhe 6 is the male parent.

[0032] The present invention also provides a DNA molecule, wherein the DNA molecule is the InDel molecular marker described above.

[0033] The present invention also provides a specific primer for identifying or assisting in the identification of wheat lateral root length and / or drought resistance. The specific primer consists of a forward primer and a reverse primer. The forward primer is a single-stranded DNA that specifically binds to the upstream of position 84 of the double-stranded DNA shown in SEQ ID No: 1 of wheat genomic DNA. The reverse primer is a single-stranded DNA that specifically binds to the downstream of position 103 of the double-stranded DNA shown in SEQ ID No: 1 of wheat genomic DNA.

[0034] The forward primer is a single-stranded DNA with the nucleotide sequence SEQ ID NO: 2, and the reverse primer is a single-stranded DNA with the nucleotide sequence SEQ ID NO: 3.

[0035] The present invention also provides a kit for identifying or assisting in the identification of wheat lateral root length and / or drought resistance, the kit containing the above-mentioned PCR primers.

[0036] The experiments of this invention demonstrate that the STS marker (PCR primers amplifying wheat genomic DNA fragments containing the InDel molecular marker) located on wheat chromosome 3B, developed based on InDel (insertion / deletion) variants, can accurately genotype allelic variants related to lateral root length and / or drought resistance, and predict wheat lateral root length and / or drought resistance. Using molecular marker-assisted selection breeding technology can effectively avoid the influence of environmental factors and human errors on phenotypic identification. The STS molecular marker of this invention can provide a strong selection signal, improve the accuracy of identifying wheat lateral root length and / or drought resistance, and achieve the goal of molecular marker-assisted selection for lateral root length and / or drought resistance. Attached Figure Description

[0037] Figure 1 STS marker gel diagrams related to lateral root length and drought resistance.

[0038] Figure 2 The total lateral root length and drought resistance index of Lumai 14 and its near-isogenic lines were determined. Detailed Implementation

[0039] The present invention will now be described in further detail with reference to specific embodiments. The given embodiments are merely illustrative of the invention and not intended to limit its scope. The embodiments provided below can serve as a guide for further improvements by those skilled in the art and do not constitute a limitation on the invention in any way.

[0040] Unless otherwise specified, the experimental methods used in the following examples are conventional methods, performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Unless otherwise specified, the materials and reagents used in the following examples are commercially available.

[0041] Example 1: Location, molecular marker, and detection method of InDel sites related to wheat lateral root length and drought resistance.

[0042] The root phenotype of wheat seedlings was identified using hydroponics. Twenty wheat varieties were used as materials. Before sowing, the seeds were disinfected with a 10% sodium hypochlorite solution, washed five times with distilled water, and then placed in petri dishes at 25℃ for 24 hours. Seeds with good emergence and uniform growth were then sown. The sown hydroponic containers were placed in an artificial climate chamber with a day / night temperature of 22℃ / 18℃ and a light intensity of 400 μmol / m². -2 s -1 On the 10th day of cultivation, the hydroponic box was removed, and the root system was scanned and the root morphology was recorded using an Epson 10000XL root scanner. The total length of the lateral roots was quantified using WinRHIZO software.

[0043] At the Shunyi Experimental Base of the Institute of Crop Science, Chinese Academy of Agricultural Sciences, two treatments were set up: dryland and irrigated land. The dryland was rainfed, while the irrigated land was irrigated (750m²) before overwintering, during the heading and flowering stages. 3 hm -2 Sow 4 rows per variety, with a row length of 2m, a row spacing of 30cm, and 40 seeds per row. Perform conventional field management, measure plot yield, and calculate drought resistance index according to the national standard "Technical Specification for Identification and Evaluation of Drought Resistance of Wheat" (GB / T 21127-2007).

[0044] PCR amplification and polypropylene thiocyanate gel electrophoresis were performed using the developed InDel marker. Based on the electrophoretic analysis, the molecular marker 3B_InDel-20bp (corresponding to the DNA fragment at positions 84-103 of SEQ ID NO: 1), which is closely linked to wheat lateral root length and drought resistance, was obtained. The following STS marker primers were designed, and their sequences are shown below:

[0045] Forward primer F: 5'-GAGTTCAGCGTCGGTCAGG-3' (SEQ ID NO: 2),

[0046] Reverse primer R: 5'-GCAGAGATGACGGAGAAAGC-3' (SEQ ID NO: 3).

[0047] Using the extracted genomic DNA of the wheat variety to be tested as a template, PCR reaction was performed using the aforementioned forward primer F and reverse primer R.

[0048] Primers were synthesized by Shanghai Sangon Biotech Co., Ltd. After synthesis, the primers were diluted to 100 μM, and the reaction mixture is shown in Table 1 below.

[0049] Table 1 PCR reaction system

[0050] DNA Template (30 ng / uL) 0.4 <![CDATA[Primer F(10pmolμl -1 )]]> 0.6 <![CDATA[Primer R(10pmol μl -1 )]]> 0.6 dNTPs (2mM) 4 2×KOD buffer 10 <![CDATA[KOD FX(1Uμl -1 )]]> 0.4 <![CDATA[ddH2O]]> 4 Total 20

[0051] The PCR amplification conditions were as follows: 95℃ for 5 min; 95℃ for 1 min, 54℃ for 1 min, 60℃ for 30 s (35 cycles); 68℃ for 10 min. The PCR products were detected by electrophoresis on a 4.0% agarose gel. A sequence length of 166 bp (SEQ ID NO: 1) indicates an insertion (In) allelic variant, and a sequence length of 146 bp (SEQ ID NO: 4) indicates a deletion (Del) allelic variant. Figure 1 ).

[0052] 5'-GAGTTCAGCGTCGGTCAGGAAGACGGCGGCCAGCCGAGGCCGGTTCTTTGCCTCTGCAAGATCTACCAGA GCCCGCGATTCCTCAAGTCCGCCTCCAAGAACTCCGCGTCCGCGCGCAAGAGGAAGACGCCGGACGACGGCCCT CGGCTTTCTCCGTCATCTCTGC-3' (SEQ ID NO: 1),

[0053] 5'-GAGTTCAGCGTCGGTCAGGAAGACGGCGGCCAGCCGAGGCCGGTTCTTTGCCTCTGCAAGATCTACCAGA GCCCGCGATTCCTCCGCGTCCGCGCGCAAGAGGAAGACGCCGGACGACGGCCCTCGGCTTTCTCCGTCATCTCT GC-3' (SEQ ID NO: 4).

[0054] Based on the root system test results of 20 wheat varieties (Table 2), it can be seen that the average total lateral root length and drought resistance index of wheat germplasm with amplification product sequence 1 are greater than those of wheat germplasm with amplification product sequence 4.

[0055] Table 2. STS marker detection results and corresponding total lateral root length and drought resistance index

[0056]

[0057]

[0058] Therefore, the lateral root length and drought resistance of wheat can be detected using forward primer F and reverse primer R. The specific method is as follows:

[0059] The wheat samples were subjected to PCR reaction using forward primer F and reverse primer R, and the sequence length of the PCR product was detected. The total lateral root length and drought resistance index of the wheat samples with a PCR product sequence length of 166 bp (SEQ ID No: 1) were greater than those of the wheat samples with a PCR product sequence length of 146 bp (SEQ ID No: 4).

[0060] Example 2: A method for identifying wheat lateral root length and drought resistance using specific primer pairs for detecting the InDel molecular marker (3B_InDel).

[0061] This embodiment uses specific primers for detecting the InDel molecular marker (3B_InDel), consisting of forward primer F and reverse primer R from Example 1, to identify wheat lateral root length and drought resistance. The specific experimental method is as follows:

[0062] Lumai 14 was used as the female parent and Shanhe 6 as the male parent for hybridization to obtain F1 seeds. F1 seeds were planted to obtain F1 individual plants. These F1 individual plants were backcrossed with Lumai 14 to obtain BC1F1 seeds. BC1F1 individual plants underwent two rounds of self-pollination to obtain BC1F3 seeds. The total lateral root length of 100 BC1F3 seedlings was identified using the above hydroponic method. Simultaneously, leaves from 100 seedling lines (BC1F3-1 to BC1F3-100) were taken. Using the genomic DNA from these leaves as a template, and using the forward primer F and reverse primer R from Example 1 as primers, PCR amplification was performed according to the PCR system and PCR amplification reaction conditions of Example 1 to obtain the amplified products. The amplification products were detected by gel electrophoresis, and the PCR products were recovered and sequenced. The sequencing results showed that the amplification products of 47 BC1F1 single plants were all DNA fragments with nucleotide sequences of SEQ ID No: 1 in the sequence listing, and their genotypes were named insertion type (In); the amplification products of 53 BC1F1 single plants were all DNA fragments with nucleotide sequences of SEQ ID No: 4 in the sequence listing, and their genotypes were named deletion type (Del).

[0063] BC1F3 seeds were planted at the Shunyi Experimental Base of the Institute of Crop Science, Chinese Academy of Agricultural Sciences. Rows were 2m long with a spacing of 0.3m, and 20 holes were sown at equal intervals per row, with 3 seeds per hole. Seedlings were thinned to three leaves to ensure one seedling per hole. The soil in the experimental plot was sandy loam. Irrigation was carried out before winter, at the jointing stage, and during the flowering stage, with each irrigation using 750m³ of water. 3 ha -1 Other management practices were the same as conventional field management. Zhongmai 36, the control variety for the drought-prone winter wheat region in the Huang-Huai area, was used as the control variety. The drought resistance index was calculated according to the national standard "Technical Specification for Identification and Evaluation of Drought Resistance of Wheat" (GB / T 21127-2007). The results are shown in Table 3.

[0064] Table 3. Genotypes, total lateral root length, and drought resistance index of 100 BC1F3 individual plants.

[0065]

[0066]

[0067] Data were processed using SPSS 19.0 statistical software. Experimental results are expressed as mean ± standard deviation. One-way ANOVA was used to analyze the relationship between the insertion and deletion genotypes and the total lateral root length and drought resistance index of wheat. The results are shown in Table 4, indicating that the total lateral root length and drought resistance index of wheat with the insertion genotype were significantly higher than those with the deletion genotype.

[0068] Table 4. Association analysis of wheat InDel molecular marker genotypes with total lateral root length and drought resistance index.

[0069] Insert type 47 <![CDATA[100.7 a ]]> <![CDATA[1.1 a ]]> Missing type 53 <![CDATA[61.7 b ]]> <![CDATA[0.94 b ]]>

[0070] Note: Different superscript letters indicate significant differences (P<0.01).

[0071] Example 3: Application of STS markers in assisted selection of wheat germplasm with long lateral roots and drought resistance

[0072] Three near-isogenic lines (NILs) of Lumai 14 (recipient parent) were screened from the backcross population of (Lumai 14 × Shanhe 6) × Lumai 14 (BC3F5) as follows: NIL1 I n, NIL2 I n and NIL3 I n: Using Lumai 14 as the female parent and Shanhe 6 as the male parent, cross them to obtain F1 seeds. Plant the F1 seeds to obtain F1 individual plants. Backcross the F1 individual plants with Lumai 14 to obtain BC1F1 seeds.

[0073] BC1F1 seeds were planted to obtain BC1F1 generation single plants. Using the genomic DNA from the leaves of these single plants as templates, and using the forward primer F and reverse primer R from Example 1 as primers, PCR amplification was performed according to the PCR system and PCR amplification reaction conditions of Example 1 to obtain the amplification product. The amplification product was detected by gel electrophoresis. The BC1F1 generation single plant with a PCR product of 166 bp was selected and backcrossed with Lumai 14 to obtain BC2F1 seeds.

[0074] BC2F1 seeds were planted to obtain BC2F1 generation single plants. Using the genomic DNA from the leaves of these single plants as templates, and using the forward primer F and reverse primer R from Example 1 as primers, PCR amplification was performed according to the PCR system and PCR amplification reaction conditions of Example 1 to obtain the amplification product. The amplification product was detected by gel electrophoresis. The BC2F1 generation single plant with a PCR product of 166 bp was selected and backcrossed with Lumai 14 to obtain BC3F1 seeds.

[0075] Plant BC3F1 seeds to obtain BC3F1 generation single plants. Select BC3F1 generation single plants with PCR product of 166bp according to the above method for self-pollination to obtain BC3F2 seeds.

[0076] Plant BC3F2 seeds to obtain BC4F2 generation single plants. Select BC3F2 generation single plants with PCR product of 166bp according to the above method for self-pollination to obtain BC3F3 seeds.

[0077] Plant BC3F3 seeds to obtain BC3F3 generation single plants. Select BC3F3 generation single plants with PCR product of 166bp according to the above method for self-pollination to obtain BC3F4 seeds.

[0078] BC3F4 seeds were planted to obtain BC3F4 generation single plants. Following the method described above, BC3F4 generation single plants with a PCR product of 166 bp were selected for self-pollination to obtain BC3F5 seeds. Three near-isogenic lines (NILs) were obtained: NIL1 In NIL2 In and NIL3 In .

[0079] NIL1 In NIL2 In and NIL3 In BC3F5 seeds were planted at the Shunyi Experimental Base of the Institute of Crop Science, Chinese Academy of Agricultural Sciences, in 2023-2024. Each material was sown in 4-row plots, with a row length of 2m, a row spacing of 0.6m, and 20 holes at equal intervals per row, with 3 seeds per hole. Seedlings were thinned to three-leaf stage to ensure one seedling per hole. A randomized block design with three replicates was used. Irrigation was conducted before overwintering, at the jointing stage, and at the flowering stage, with each irrigation using 750m³ of water. 3 ha -1 Other management practices were the same as in conventional field management. Yield of each plot was measured after maturity. Using the forward primer F and reverse primer R from Example 1 as primers, PCR amplification was performed according to the PCR system and PCR amplification reaction conditions of Example 1 to obtain the amplification products. The amplification products were detected by gel electrophoresis, and the PCR products were recovered and sequenced. Sequencing results showed NIL1. In NIL2 In and NIL3 In The amplification products of each of the five individual plants were all DNA fragments with nucleotide sequences of SEQ ID No: 1 in the sequence listing, and their genotypes were insertion type (In); the amplification products of the five individual plants of Lumai 14 were all DNA fragments with nucleotide sequences of SEQ ID No: 4 in the sequence listing, and their genotypes were deletion type (Del).

[0080] Lateral root lengths of the recipient parent Lumai 14 and three near-isogenic lines were identified using the same method as in Example 1. The results are as follows: Figure 2 As shown in the figure, the total lateral root length of Lumai 14 and the three near-isogenic lines showed extremely significant differences (P<0.01) through analysis of variance. The total lateral root length and drought resistance index of Lumai 14, with an amplified product sequence length of 146 bp, were 52.7 cm and 0.92, respectively, while those of the near-isogenic line NIL1, with an amplified product sequence length of 166 bp, were significantly different. In NIL2 InNIL3 In The total lateral root length and drought resistance index were 67cm, 75cm, and 73.7cm, and 1.10, 1.14, and 1.13, respectively. Figure 2 ).

[0081] This shows that wheat with the 3B_InDel-20bp genome has significantly higher lateral root length and drought resistance index than wheat without it. This indicates that the presence of the 3B_InDel-20bp sequence in the wheat genome can be used to predict wheat lateral root length and drought resistance index. Therefore, detecting the presence of the 3B_InDel-20bp sequence in the wheat genome can rapidly and accurately identify wheat lateral root length and drought resistance index.

[0082] In the breeding of wheat varieties with excellent root traits, it is best to select wheat with the genotype of insertion containing 3B_InDel-20bp as the parent for breeding.

[0083] The PCR product sequence length using the genomic DNA of the parental line Lumai 14 as a template was 146 bp, and the PCR product sequence length using the genomic DNA of near-isogenic line materials as a template was 166 bp.

[0084] The STS molecular markers of this invention have clearly defined sequences and primers, and based on case studies, can be directly used in marker-assisted selection breeding.

[0085] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

[0086] The present invention has been described in detail above. Those skilled in the art will recognize that the invention can be practiced in a wide range of ways with equivalent parameters, concentrations, and conditions without departing from its spirit and scope, and without requiring unnecessary experiments. While specific embodiments have been provided, it should be understood that further modifications can be made to the invention. In summary, according to the principles of the invention, this application is intended to include any changes, uses, or improvements to the invention, including changes made using conventional techniques known in the art that depart from the scope disclosed herein.

Claims

1. The application of InDel molecular markers, characterized by: The InDel molecular marker is a DNA fragment with nucleotide sequence SEQ ID NO:1 from position 82 to 101, and the application is the application of the InDel molecular marker in any of the following: A1) To identify or assist in the identification of wheat lateral root length; A2) To identify or assist in the identification of wheat drought resistance; A3) Breed wheat varieties with improved total lateral root length and / or better drought resistance index than their parents.

2. The application of detecting InDel-labeled substances, characterized in that: The InDel molecular marker is a DNA fragment with nucleotide sequence SEQ ID NO:1, positions 82-101, and the application is the use of the substance in any of the following: B1) Identification or auxiliary identification of wheat lateral root length; B2) Prepare products for identification or auxiliary identification of wheat lateral root length; B3) Identification or auxiliary identification of wheat drought resistance; B4) Prepare products for identifying or assisting in the identification of wheat drought resistance; B5) Cultivate wheat varieties with superior total lateral root length and / or drought resistance index compared to their parents; B6) Prepare products of wheat that have improved total lateral root length and / or drought resistance index compared to the parent wheat.

3. The application according to claim 2, characterized in that, The substance contains PCR primers for amplifying wheat genomic DNA fragments including the InDel molecular marker.

4. The application according to claim 3, characterized in that, The PCR primers are primer pairs, which consist of a forward primer and a reverse primer. The forward primer is a single-stranded DNA that specifically binds to the upstream of position 82 of the double-stranded DNA shown in SEQ ID NO:1 of wheat genomic DNA, and the reverse primer is a single-stranded DNA that specifically binds to the downstream of position 101 of the double-stranded DNA shown in SEQ ID NO:1 of wheat genomic DNA.

5. The application according to claim 4, characterized in that, The forward primer is a single-stranded DNA with the nucleotide sequence SEQ ID NO:2, and the reverse primer is a single-stranded DNA with the nucleotide sequence SEQ ID NO:

3.

6. A method for identifying or assisting in the identification of wheat lateral root length and / or drought resistance, comprising using the genomic DNA of the wheat to be identified as a template, performing PCR amplification with the PCR primers described in claims 3, 4 or 5 to obtain PCR products, and identifying wheat lateral root length and / or drought resistance based on whether the PCR products contain the InDel molecular marker described in claim 1.

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