Microfluidic intracellular delivery device and delivery method based on 3D electroporation system

Abstract

The invention discloses a microfluidic intracellular delivery device and delivery method based on a 3D electroporation system, and relates to the technical field of intracellular delivery, the microfluidic intracellular delivery device comprises a 3D electroporation microfluidic chip, a power supply and a pressure pump; the 3D electroporation micro-fluidic chip is respectively connected with the power supply and the pressure pump; the 3D electroporation micro-fluidic chip comprises channel inlets and outlets and shrinkage channels alternately distributed in the middle, the channel inlets and outlets are located in an inlet and an outlet of the chip respectively and used for allowing cells to enter the chip and collecting the cells, and the shrinkage channels alternately distributed in the middle are channels with different inner diameters and alternately distributed; the power supply is used for supplying power to the 3D electroporation micro-fluidic chip, so that an electric field is generated in a channel, and the pressure pump is used for conveying cells to pass through the chip at different flow speeds and collecting the cells. The method can efficiently complete high-activity delivery of allogenic substances to different types of cells, has excellent delivery efficiency and biocompatibility, and effectively solves the problems of complex operation procedures and high cytotoxicity of the current intracellular delivery method.

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