A SERS-based digital nucleic acid detection platform, its preparation method, and its application

By using a SERS-based digital nucleic acid detection platform and CRISPR/Cas12a technology, we have achieved high-sensitivity detection of the COX III gene, solving the problems of long processing time and cumbersome operation of existing methods. This method is suitable for early diagnosis of acute kidney injury and detection of specific cancer biomarkers.

CN119614681BActive Publication Date: 2025-10-28HAINAN MEDICAL UNIV
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Patent Information

Application Number
CN202411858634.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-12-17
Publication Date
2025-10-28
Estimated Expiration
2044-12-17

AI Technical Summary

Technical Problem

Existing COX III gene detection methods are time-consuming, have limited sensitivity, and are cumbersome to operate, making it difficult to meet the needs of early diagnosis of acute kidney injury.

Method used

A SERS-based digital nucleic acid detection platform, combined with CRISPR/Cas12a technology, was used to achieve absolute quantitative analysis of target genes through microfluidic chips and SERS probes. Cas12a was used to form a binary complex with crRNA to capture and cleave target DNA, which was then combined with magnetic separation and Raman signal detection.

Benefits of technology

It achieves a sensitivity of 10 fM for COX III gene detection, simplifies sample processing, improves detection efficiency, is suitable for resource-limited environments, and is applicable to the early diagnosis of acute kidney injury and the detection of specific cancer biomarkers.

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Abstract

This invention discloses a SERS-based digital nucleic acid detection platform, its preparation method, and its applications, relating to the field of nucleic acid detection technology. It includes a microfluidic chip, a SERS probe, Cas12a, crRNA, and a Raman signal detector. The SERS probe is formed by base pairing of Au@DNA and MB@BiotinDNA. The microfluidic chip is provided with sequentially distributed and interconnected droplet generation region, droplet electrode fusion region, reservoir, magnetic separation region, and detection cell. The droplet generation region is used to divide the sample to be tested into several droplets. The droplet electrode fusion region is provided with channels for adding the SERS probe, Cas12a, and crRNA. The digital nucleic acid detection platform provided by this invention has a detection limit as low as 10 fM; it reduces the sample processing process and improves the sample detection efficiency; it can be operated directly at room temperature without a complex operating environment, making it convenient to use.
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Citation Information

Patent Citations

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