Primer pair for identifying ulmus pumila and peeled ulmus pumila germplasm and application thereof

Abstract

The invention discloses a primer pair for identifying germplasm of ulmus pumila and peeled ulmus pumila and application of the primer pair, the primer pair comprises the following primer pairs Ulmusp20, Ulmusp61 or / and Ulmusp165, a fresh tissue sample of ulmus pumila or peeled ulmus pumila to be detected is collected, and DNA of the tissue sample is extracted; carrying out PCR (Polymerase Chain Reaction) amplification by using the primer pair by taking the DNA as a template; carrying out electrophoresis detection on an amplification product; judging whether the sample to be detected is ulmus pumila or peeled ulmus pumila according to a detection result. The SSR molecular marker for transcriptome sequencing is used for identifying germplasm resources of the ulmus pumila and the peeled ulmus pumila, so that improved variety breeding of the ulmus pumila and the peeled ulmus pumila is promoted, and the utilization process of excellent germplasm is accelerated. The specific primers respectively amplify peeled elm and ulmus pumila tissues, and the length difference of the two products is significant, which indicates that the specific primers can effectively identify the germplasm of ulmus pumila and peeled elm.

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