Cosmetic containing nucleic acid composition as well as preparation method and application thereof

Abstract

The invention belongs to the field of cosmetics, and relates to a cosmetic containing nucleic acid composition substances as well as a preparation method and application thereof. The cosmetic containing the nucleic acid component is prepared from the following components in parts by weight: 60.0 to 65.0 parts of water; 0.5 to 1.5 parts of a nucleic acid composition substance; 4.0 to 7.0 parts of polydimethylsiloxane; 4.0 to 7.0 parts of cyclopentasiloxane (cyclopentasiloxane); 14.5 to 21.5 parts of a humectant; 1.0 to 1.5 parts of lauryl alcohol; 1.0 to 2.0 parts of polyacrylamide; 2.0 to 3.0 parts of an emulsifier; 0.2 to 2.0 parts of a preservative; and 0.1 to 1.0 part of essence. The cosmetic prepared by adding nucleic acid composition substances is non-irritant to skin, and has good moisture retention, oxidation resistance, sunscreen, wrinkle fading and other effects.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics, and relates to a cosmetic containing nucleic acid constituent substances, and a preparation method and application thereof. Background Art

[0002] Nucleotides are important molecules in organisms and are involved in a variety of biological processes, such as energy metabolism, signal transduction, and gene expression. In recent years, the application of nucleotides in the field of cosmetics has gradually attracted attention, especially in anti-aging, moisturizing, and repairing.

[0003] Adenosine was included in the "Catalogue of Approved Cosmetic Raw Materials (First Batch)" published by the State Food and Drug Administration in February 2013, which means that adenosine in a certain concentration can be added to cosmetics.

[0004] Adenosine, English name (INCI): ADENOSINE, is an endogenous nucleoside that is distributed throughout human cells. It has anti-aging effects in cosmetics. Adenosine is used as an antioxidant, skin conditioner, and moisturizer in cosmetics. The State Food and Drug Administration included adenosine in the "Catalogue of Approved Cosmetic Ingredients (First Batch)" published in February 2013, which means that a certain concentration of adenosine can be added to cosmetics. Adenosine is an anti-aging introducer that can replenish skin energy, significantly improve skin elasticity, promote skin cell metabolism, prevent skin tissue from sagging, and reorganize skin structure, so that the skin can restore elasticity by tightening and shrinking pores. Adenosine ingredients are suitable for wrinkled skin, sensitive skin, oily skin, pigmented skin, dry skin and other skin types.

[0005] Cytidine is distributed in all organisms. It is an important component of cells and participates in their metabolism. Cytidine is currently mainly produced by fermentation. Cytidine is a nutrient. It can reduce the depth of wrinkles and improve the texture of the skin when used in skin care products. It can also be used as a hair growth agent. CTFA lists cytidine as a cosmetic raw material in the 2010 edition of the "International Standard Chinese Name Catalog of Cosmetic Raw Materials" published by the China Association of Perfumery and Aromatics.

[0006] Cytosine: The "Notice on the Catalog of Used Cosmetic Ingredients" issued by the State Food and Drug Administration in 2014, and the "International Standard Chinese Name Catalog of Cosmetic Ingredients" published by CTFA and the China Perfume and Chemical Association in 2010 all list cytosine as a cosmetic ingredient, and there are no reports that it is unsafe for external use. Cytosine is a component of nucleic acid, participates in the biochemical activities of the human body, can be used as a nutrient, has a conditioning effect on the skin, and can be used in hair products to promote hair growth; it can replace inorganic alkali to adjust the pH value of creams, which helps to relieve skin allergies caused by this aspect; cytosine has a sunscreen effect and has a cool feeling when applied to the skin.

[0007] Guanosine: Guanosine can participate in various cell activities and is an important biochemical substance in many biochemical processes of the human body. External use can directly and quickly replenish cell energy, promote the biosynthesis of skin collagen, elastin and fibrin, and is a cell activator. It is used for multi-faceted skin conditioning and has anti-inflammatory effects. When used in nail products, it can reduce nail brittleness and improve its toughness. CTFA lists guanosine as a cosmetic raw material in the 2010 edition of the "International Cosmetic Raw Materials Standard Chinese Name Catalog" of the China Perfume Association. Summary of the invention

[0008] Purpose of the invention: The technical problem to be solved by the present invention is to provide a cosmetic containing nucleic acid components and a preparation method and application thereof in view of the deficiencies of the prior art. The present invention achieves a synergistic effect by mixing different types of nucleic acid components in a specific ratio; the cosmetics prepared by adding nucleic acid components in the present invention have excellent moisturizing, repairing, anti-aging, wrinkle-reducing and sunscreen effects, and are suitable for all skin types, especially dry and sensitive skin.

[0009] In order to solve the above technical problems, the technical solution adopted by the present invention is as follows:

[0010] The present invention discloses a cosmetic containing nucleic acid constituent material, which is composed of the following components in parts by weight:

[0011] Water 60.0-65.0 parts;

[0012] 0.5-1.5 parts of nucleic acid constituent substances;

[0013] 4.0-7.0 parts of polydimethylsiloxane;

[0014] 4.0-7.0 parts of cyclopentasiloxane;

[0015] 14.5-21.5 parts of moisturizer;

[0016] 1.0-1.5 parts of lauryl alcohol;

[0017] 1.0-2.0 parts of polyacrylamide;

[0018] Emulsifier 2.0-3.0 parts;

[0019] 0.2-2.0 parts of preservatives;

[0020] 0.1-1.0 parts of flavor.

[0021] The cosmetics containing nucleic acid constituent substances provided by the present invention do not contain unsaturated fatty acids, saturated fatty acids, or protein substances.

[0022] In some embodiments, preferably, the cosmetics containing nucleic acid constituent substances are composed of the following components in parts by weight:

[0023] 62.5 parts of water;

[0024] 1.0 part of nucleic acid constituent material;

[0025] 6.0 parts of polydimethylsiloxane;

[0026] Cyclopentasiloxane 6.0 parts;

[0027] Moisturizer 19.0 parts;

[0028] 1.3 parts of lauryl alcohol;

[0029] 1.7 parts of polyacrylamide;

[0030] 2.5 parts of emulsifier;

[0031] 0.6 parts of preservatives;

[0032] 0.4 parts of flavor.

[0033] In some embodiments, the moisturizer is any one or a combination of glycerin, 1,3-propylene glycol, panthenol, hyaluronic acid, sodium hyaluronate, urea and ceramide; the emulsifier is any one or a combination of stearyl alcohol polyether, oleyl alcohol polyether, glyceryl stearate and cetyl polyethylene glycol; the preservative is any one or a combination of phenoxyethanol, parabens and parahydroxyacetophenone.

[0034] In some embodiments, preferably, the moisturizer is any one or a combination of glycerol, 1,3-propylene glycol and hyaluronic acid; the emulsifier is glyceryl stearate; and the preservative is p-hydroxyacetophenone.

[0035] In some embodiments, preferably, the cosmetic is composed of the following components in parts by weight:

[0036] Water 60.0-65.0 parts;

[0037] 0.5-1.5 parts of nucleic acid constituent substances;

[0038] 4.0-7.0 parts of polydimethylsiloxane;

[0039] 4.0-7.0 parts of cyclopentasiloxane;

[0040] Glycerin 7.0-10.0 parts;

[0041] 7.0-10.0 parts of 1,3-propylene glycol;

[0042] 0.5-1.5 parts of hyaluronic acid;

[0043] 1.0-1.5 parts of lauryl alcohol;

[0044] 1.0-2.0 parts of polyacrylamide;

[0045] Glyceryl stearate 2.0-3.0 parts;

[0046] 0.2-2.0 parts of p-hydroxyacetophenone;

[0047] 0.1-1.0 parts of flavor.

[0048] In some embodiments, further preferably, the cosmetic is composed of the following components in parts by weight:

[0049] 62.5 parts of water;

[0050] 1.0 part of nucleic acid constituent material;

[0051] 6.0 parts of polydimethylsiloxane;

[0052] Cyclopentasiloxane 6.0 parts;

[0053] Glycerin 9.0 parts;

[0054] 9.0 parts of 1,3-propylene glycol;

[0055] 1.0 part of hyaluronic acid;

[0056] 1.3 parts of lauryl alcohol;

[0057] 1.7 parts of polyacrylamide;

[0058] 2.5 parts of glyceryl stearate;

[0059] 0.6 parts of p-hydroxyacetophenone;

[0060] 0.4 parts of flavor.

[0061] In some embodiments, the nucleic acid constituent substance is any one or a combination of guanosine, adenosine, cytidine and cytosine.

[0062] In some embodiments, when the nucleic acid constituent material is a combination of guanosine and adenosine, the weight ratio of guanosine to adenosine is (1:2) to (2:1).

[0063] In some embodiments, when the nucleic acid constituent material is a combination of guanosine and cytidine, the weight ratio of guanosine to cytidine is (1:2) to (2:1).

[0064] In some embodiments, when the nucleic acid constituent material is a composition of adenosine and cytidine, the weight ratio of adenosine to cytidine is (1:2) to (2:1).

[0065] In some embodiments, when the nucleic acid constituent material is a composition of guanosine, adenosine and cytidine, the weight ratio of guanosine, adenosine and cytidine is (1-3): (1-2): (1-3).

[0066] In some embodiments, when the nucleic acid constituent material is a composition of guanosine, adenosine, cytidine and cytosine, the weight ratio of guanosine, adenosine, cytidine and cytosine is (1-4):(1-3):(1-2):1.

[0067] In some embodiments, preferably, when the nucleic acid constituent material is a composition of guanosine and adenosine, the weight ratio of guanosine to adenosine is 1:1.

[0068] In some embodiments, preferably, when the nucleic acid constituent material is a composition of guanosine and cytidine, the weight ratio of guanosine to cytidine is 1:1.

[0069] In some embodiments, preferably, when the nucleic acid constituent material is a composition of adenosine and cytidine, the weight ratio of adenosine to cytidine is 1:1.

[0070] In some embodiments, preferably, when the nucleic acid constituent substance is a composition of guanosine, adenosine and cytidine, the weight ratio of guanosine, adenosine and cytidine is 1:1:1.

[0071] In some embodiments, preferably, when the nucleic acid constituent material is a composition of guanosine, adenosine, cytidine and cytosine, the weight ratio of guanosine, adenosine, cytidine and cytosine is 1:1:1:1.

[0072] Furthermore, the present invention discloses a method for preparing the above-mentioned cosmetics, comprising the following steps:

[0073] (1) Dissolving: mixing the water, moisturizing agent and nucleic acid constituent material in the above-mentioned weight proportions, stirring and heating for emulsification to obtain an aqueous phase;

[0074] (2) mixing the oil phase: uniformly mixing the polydimethylsiloxane, cyclopentasiloxane, lauryl alcohol, emulsifier and polyacrylamide in the above-mentioned weight proportions to obtain an oil phase;

[0075] (3) emulsification: mixing the oil phase obtained in step (2) and the water phase obtained in step (1) at a controlled temperature, and then emulsifying to obtain an emulsified mixture;

[0076] (4) Aging: lowering the system temperature of the emulsified mixture obtained in step (3), adding the essence in the aforementioned weight proportion to the system; then continuing to cool to room temperature and aging to obtain the cosmetic;

[0077] The preservative in the above-mentioned weight proportion can be added in the emulsification step of step (3), or can be added together with the flavor in step (4).

[0078] In some embodiments, in step (1), the emulsification temperature is 70-85° C. and the emulsification time is 40-50 min; the stirring speed is 1000-1500 rpm.

[0079] In some embodiments, in step (1), the emulsification temperature is 80-85° C., and the emulsification time is 40-50 min; and the stirring speed is 1200 rpm.

[0080] In some embodiments, in step (2), the mixing is performed at 70-85° C.; the mixing requires stirring, the stirring speed is 800-1500 rpm, and the stirring time is 10-50 min.

[0081] In some embodiments, in step (2), the mixing is performed at 80-85° C.; the mixing requires stirring, the stirring speed is 1000 rpm, and the stirring time is 20-30 min.

[0082] In some embodiments, in step (3), the temperature is controlled at 70-75°C; the emulsification temperature is 70-75°C and the emulsification time is 20-50 min; stirring is required during the emulsification process and the stirring speed is 1000-1500 rpm.

[0083] In some embodiments, in step (3), the temperature is controlled at 73°C; the emulsification temperature is 73°C and the emulsification time is 30 to 45 minutes; stirring is required during the emulsification process and the stirring speed is 1200 rpm.

[0084] In some embodiments, in step (4), the lowering of the system temperature is performed to reduce the temperature to 30-40° C.; and the aging is performed for 2-6 hours.

[0085] In some embodiments, in step (4), the lowering of the system temperature is to lower the temperature to 39° C.; and the aging is performed for 2 to 6 hours.

[0086] The use of the above-mentioned cosmetics in the preparation of cosmetics with moisturizing and / or anti-oxidation properties and / or wrinkle-reducing and / or sun-protection functions also falls within the protection scope of the present invention.

[0087] Beneficial effects:

[0088] The cosmetics prepared by adding nucleic acid constituent substances in the present invention are non-irritating to the skin and have good moisturizing, anti-oxidation and wrinkle-reducing effects. Furthermore, by adding cytosine, the cosmetics have good sun protection effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0089] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments, and the above and / or other advantages of the present invention will become more clear.

[0090] Figure 1 This is a photo of the cosmetics obtained in Example 2 of the present invention. DETAILED DESCRIPTION

[0091] The present invention can be better understood according to the following examples. However, it is easy for those skilled in the art to understand that the contents described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

[0092] The experimental methods described in the following examples are conventional methods unless otherwise specified; the reagents and materials described are commercially available unless otherwise specified.

[0093] Embodiment 1:

[0094] Preparation of raw materials: Prepare the materials according to the table below.

[0095] Table 1

[0096]

[0097]

[0098] The cosmetics were prepared using the components with the mass contents in Table 1 as raw materials. The preparation method is as follows:

[0099] (1) Dissolution: Mix water, glycerol, 1,3-propylene glycol and hyaluronic acid, stir and heat to 81°C to 85°C in an emulsifier at a stirring speed of 1200 rpm, and emulsify for 40 to 50 minutes to obtain an aqueous phase.

[0100] (2) Mixing the oil phase: Mix polydimethylsiloxane, cyclopentasiloxane, lauryl alcohol, glyceryl stearate and polyacrylamide, stir and heat to 81°C to 85°C in an oil phase pot at a stirring speed of 1000 rpm for 20 to 30 minutes to obtain an oil phase.

[0101] (3) Emulsification: The oil phase obtained in step (2) is transferred to an emulsifying pot and mixed with the water phase obtained in step (1). The temperature is controlled at 73° C. and the stirring speed is 1200 rpm. During the stirring process, p-hydroxyacetophenone is slowly added to the mixture. The mixture is emulsified for 30 minutes to obtain an emulsified mixture.

[0102] (4) Aging: The temperature of the emulsified mixture obtained in step (3) is slowly lowered. When the temperature drops to about 39° C., flavor is added; the temperature is then further lowered to room temperature and aged for 2 to 6 hours. The product is recorded as cosmetic 1.

[0103] Embodiment 2:

[0104] Table 2

[0105]

[0106]

[0107] The cosmetics were prepared using the components with the mass contents in Table 2 as raw materials. The preparation method is as follows:

[0108] (1) Dissolution: Mix water, glycerol, 1,3-propylene glycol, hyaluronic acid, adenosine, guanosine and cytidine, stir and heat to 81°C to 85°C in an emulsifier at a stirring speed of 1200 rpm, and emulsify for 40 to 50 minutes to obtain an aqueous phase.

[0109] (2) Mixing the oil phase: Mix polydimethylsiloxane, cyclopentasiloxane, lauryl alcohol, glyceryl stearate and polyacrylamide, stir and heat to 81°C to 85°C in an oil phase pot at a stirring speed of 1000 rpm for 20 to 30 minutes to obtain an oil phase.

[0110] (3) Emulsification: The oil phase obtained in step (2) is transferred to an emulsifying pot and mixed with the water phase obtained in step (1). The temperature is controlled at 73° C. and the stirring speed is 1200 rpm. During the stirring process, p-hydroxyacetophenone is slowly added to the mixture. The mixture is emulsified for 30 minutes to obtain an emulsified mixture.

[0111] (4) Aging: The temperature of the emulsified mixture obtained in step (3) is slowly lowered. When the temperature drops to about 39° C., flavor is added; the temperature is then further lowered to room temperature and aged for 2 to 6 hours. The product is recorded as Cosmetics 2.

[0112] Example 3: Adenosine + Guanosine

[0113] Table 3

[0114]

[0115]

[0116] The cosmetics were prepared using the components with the mass contents in Table 3 as raw materials. The preparation method is as follows:

[0117] (1) Dissolution: Mix water, glycerol, 1,3-propylene glycol, hyaluronic acid, adenosine and guanosine, stir and heat to 81°C to 85°C in an emulsifier at a stirring speed of 1200 rpm, and emulsify for 40 to 50 minutes to obtain an aqueous phase.

[0118] (2) Mixing the oil phase: Mix polydimethylsiloxane, cyclopentasiloxane, lauryl alcohol, glyceryl stearate and polyacrylamide, stir and heat to 81°C to 85°C in an oil phase pot at a stirring speed of 1000 rpm for 20 to 30 minutes to obtain an oil phase.

[0119] (3) Emulsification: The temperature of the oil phase obtained in step (2) is controlled at 73° C. and the stirring speed is 1200 rpm. During the stirring process, the water phase obtained in step (1) is slowly added to the oil phase, and the temperature and stirring speed are maintained. Emulsification is performed for 45 minutes to obtain an emulsified mixture.

[0120] (4) Aging: The temperature of the emulsified mixture obtained in step (3) is slowly lowered. When the temperature drops to about 39° C., flavor and p-hydroxyacetophenone are added. The temperature is then further lowered to room temperature and aged for 2 to 6 hours. The product is recorded as Cosmetics 3.

[0121] Example 4: Adenosine + Cytidine

[0122] Table 4

[0123]

[0124]

[0125] The cosmetics were prepared using the components with the mass contents in Table 4 as raw materials. The preparation method is as follows:

[0126] (1) Dissolution: Mix water, glycerol, 1,3-propylene glycol, hyaluronic acid, adenosine and cytidine, stir and heat to 81°C to 85°C in an emulsifier at a stirring speed of 1200 rpm, and emulsify for 40 to 50 minutes to obtain an aqueous phase.

[0127] (2) Mixing the oil phase: Mix polydimethylsiloxane, cyclopentasiloxane, lauryl alcohol, glyceryl stearate and polyacrylamide, stir and heat to 81°C to 85°C in an oil phase pot at a stirring speed of 1000 rpm for 20 to 30 minutes to obtain an oil phase.

[0128] (3) Emulsification: The temperature of the oil phase obtained in step (2) is controlled at 73° C. and the stirring speed is 1200 rpm. During the stirring process, the water phase obtained in step (1) is slowly added to the oil phase, and the temperature and stirring speed are maintained. Emulsification is performed for 45 minutes to obtain an emulsified mixture.

[0129] (4) Aging: The temperature of the emulsified mixture obtained in step (3) is slowly lowered. When the temperature drops to about 39° C., flavor and p-hydroxyacetophenone are added. The temperature is then further lowered to room temperature and aged for 2 to 6 hours. The product is recorded as cosmetic 4.

[0130] Example 5: Guanosine + Cytidine

[0131] Table 5

[0132]

[0133]

[0134] The cosmetics were prepared using the components with the mass contents in Table 5 as raw materials. The preparation method is as follows:

[0135] (1) Dissolution: Mix water, glycerol, 1,3-propylene glycol, hyaluronic acid, guanosine and cytidine, stir and heat to 81°C to 85°C in an emulsifier at a stirring speed of 1200 rpm, and emulsify for 40 to 50 minutes to obtain an aqueous phase.

[0136] (2) Mixing the oil phase: Mix polydimethylsiloxane, cyclopentasiloxane, lauryl alcohol, glyceryl stearate and polyacrylamide, stir and heat to 81°C to 85°C in an oil phase pot at a stirring speed of 1000 rpm for 20 to 30 minutes to obtain an oil phase.

[0137] (3) Emulsification: The temperature of the oil phase obtained in step (2) is controlled at 73° C. and the stirring speed is 1200 rpm. During the stirring process, the water phase obtained in step (1) is slowly added to the oil phase, and the temperature and stirring speed are maintained. Emulsification is performed for 45 minutes to obtain an emulsified mixture.

[0138] (4) Aging: The temperature of the emulsified mixture obtained in step (3) is slowly lowered. When the temperature drops to about 39° C., flavor and p-hydroxyacetophenone are added. The temperature is then further lowered to room temperature and aged for 2 to 6 hours. The product is recorded as cosmetic 5.

[0139] Embodiment 6:

[0140] 1. Preparation of cosmetics:

[0141] The preparation method of the cosmetics is the same as that of Example 2, and the material list of the cosmetics is as follows:

[0142] Table 6

[0143]

[0144]

[0145] The specific ratios and experimental schemes of the nucleic acid components in Table 6 are as follows:

[0146] Table 7

[0147]

[0148] 2. Security Testing

[0149] The cosmetics containing nucleic acid components (different cosmetics prepared by the experimental schemes in Table 7 of this embodiment) were tested for safety such as skin irritation and allergy to ensure that the products would not cause adverse reactions to the skin during use.

[0150] (1) Test materials and methods:

[0151] Animals: Common experimental animals are white rabbits. No abnormalities were found in the animals during physical examination.

[0152] Test method: According to the acute skin irritation test of the "Hygienic Standards for Cosmetics" (2007 edition), 24 hours before the test, the hair on both sides of the spine of the test animal's back was cut off, and the hair removal area was 3 cm × 3 cm. Take 0.5g of cosmetics without nucleic acid components or each group of cosmetics containing nucleic acid components prepared by each experimental scheme in Example Table 7 and apply it directly on the right skin, cover it with clean gauze, and fix it with non-irritating adhesive tape for 2 hours. The other side is a negative control. Observe the skin reaction of the contact part of the test animal 1 hour, 24 hours, 48 ​​hours, and 72 hours after removing the test substance and make a record.

[0153] (2) Conclusion: Under the conditions of this test, the cosmetics containing the nucleic acid constituents of the present invention were classified as non-irritating in the acute skin irritation test on rabbits according to the skin irritation intensity. The control cosmetics (cosmetic base without nucleic acid constituents) were also non-irritating.

[0154] 3. Moisturizing test, free radical scavenging rate test and wrinkle reduction test

[0155] (1) Skin moisturizing test:

[0156] Evaluate the effect of cosmetics containing nucleic acid components on skin moisture content through in vitro or in vivo experiments. Use tools such as a skin moisture tester to measure skin moisture content before and after use and compare changes.

[0157] Cosmetic moisturizing degree and moisturizing time detection: MMV (moisture measurement value) method detects the MMV value, which reflects the ability of the skin stratum corneum to bind water. The CM825 skin oil, water, and acid-base tester is used to test the hydration of the skin. Its measurement principle is that the stratum corneum contains a large amount of electrolytes, and the electrolytes in the water are conductive. This phenomenon also occurs on the surface of the skin. After the test probe comes into contact with the skin, the change in conductivity reflects the hydration state of the skin.

[0158] The subjects were healthy volunteers without skin diseases. 90 healthy volunteers without skin diseases were selected and divided into 9 groups according to the experimental schemes in Table 7, with 10 people in each group for testing. The test method was as follows: ① Determine 4 test areas on the left and right forearms, with an area of ​​4cm×4cm, and test the blank value of each area; ② Apply the sample to the test area at a dosage of 2mg / cm 2 ; ③ Perform the final test 120 minutes after use, test 5 points in each area and take the average value. The samples are different cosmetics prepared by the experimental schemes in Table 7. The evaluation formula for moisturizing effect evaluation is as follows:

[0159] Relative moisture retention rate / %=(P sample t=xP blank) / (P standard t=xP blank)×100%;

[0160] Among them, Psample t=x is the conductivity of the product after a certain period of use; Pblank is the conductivity of the uncoated sample; and Pstandard t=x is the conductivity of the reference (a cosmetic matrix that does not contain nucleic acid components) after a certain period of use.

[0161] (2) Free radical scavenging rate test

[0162] DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) is a stable free radical. Its solution is purple and has a maximum absorption peak at 517nm. When an antioxidant is added to the DPPH solution, the DPPH free radical is scavenged, the solution color becomes lighter, and the absorbance decreases. The degree of change is linearly related to the degree of free radical scavenging, so the activity of the antioxidant can be evaluated. Detection method: DPPH free radical scavenging rate detection kit (colorimetric method).

[0163] (3) Wrinkle reduction test

[0164] Wrinkle removal effect: Volunteers (18-45 years old, 90 people) were selected and divided into 9 groups according to the experimental schemes in Table 7, with 10 people in each group for testing. The prepared cosmetics were applied mainly to the skin where wrinkles just appeared on the face, and used continuously for one month.

[0165] Table 8 Wrinkle removal effect evaluation table

[0166]

[0167] A 10-point system was adopted, with a full score of 10 for each item. 90 volunteers were invited to evaluate their hobbies, and finally the average score of each group was taken as the evaluation result.

[0168] 4. Results:

[0169] Table 9

[0170]

[0171] Embodiment 7:

[0172] Table 10

[0173]

[0174] (1) Dissolution: Mix water, glycerol, 1,3-propylene glycol, hyaluronic acid, guanosine, cytidine, adenosine and cytosine, stir and heat to 81°C to 85°C in an emulsifier at a stirring speed of 1200 rpm, and emulsify for 40 to 50 minutes to obtain an aqueous phase.

[0175] (2) Mixing the oil phase: Mix polydimethylsiloxane, cyclopentasiloxane, lauryl alcohol, glyceryl stearate and polyacrylamide, stir and heat to 81°C to 85°C in an oil phase pot at a stirring speed of 1000 rpm for 20 to 30 minutes to obtain an oil phase.

[0176] (3) Emulsification: The temperature of the oil phase obtained in step (2) is controlled at 73° C. and the stirring speed is 1200 rpm. During the stirring process, the water phase obtained in step (1) is slowly added to the oil phase, and the temperature and stirring speed are maintained. Emulsification is performed for 45 minutes to obtain an emulsified mixture.

[0177] (4) Aging: Slowly lower the system temperature of the emulsified mixture obtained in step (3), and when the temperature drops to about 39° C., add flavor and p-hydroxyacetophenone; then continue to cool to room temperature and age for 2 to 6 hours to obtain cosmetic 6.

[0178] Embodiment 8:

[0179] The sunscreen performance of the product was tested by testing the cosmetic 2 prepared in Example 2 and the cosmetic 6 prepared in Example 7. The specific testing method is as follows:

[0180] (1) Accurately weigh 200 mg of each cosmetic sample into a 100 mL bottle, add anhydrous ethanol and anhydrous ether in a 1:1 ratio as solvent to prepare a solution. Pour the prepared solution into a 1 cm × 1 cm × 5 cm quartz colorimetric cell, and measure the ultraviolet transmittance in the wavelength range of 290 to 400 nm in a tuned ultraviolet spectrophotometer.

[0181] (2) The test results are shown in the following table.

[0182] Table 11

[0183] Test items UVB transmittance UVA transmittance Adenosine + Cytidine + Guanosine 3.2517 17.8458 Adenosine + Cytidine + Guanosine + Cytosine 1.9943 9.8912

[0184] From the data in the table, we can see that adding cytosine to cosmetic 2 can effectively enhance the sun protection effect of the cosmetic.

[0185] The present invention provides a cosmetic containing nucleic acid components and a preparation method and application thereof. There are many methods and approaches to implement the technical solution. The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention. All components not specified in this embodiment can be implemented by existing technologies.

Claims

1. A cosmetic containing nucleic acid components, characterized in that: It is composed of the following components in parts by weight: Water 60.0-65.0 parts; 0.5-1.5 parts of nucleic acid constituent substances; 4.0-7.0 parts of polydimethylsiloxane; 4.0-7.0 parts of cyclopentasiloxane; 14.5-21.5 parts of moisturizer; 1.0-1.5 parts of lauryl alcohol; 1.0-2.0 parts of polyacrylamide; Emulsifier 2.0-3.0 parts; 0.2-2.0 parts of preservatives; 0.1-1.0 parts of flavor.

2. The cosmetic according to claim 1, characterized in that: The moisturizer is any one of glycerin, 1,3-propylene glycol, panthenol, hyaluronic acid, sodium hyaluronate, urea and ceramide, or a combination of several of them; the emulsifier is any one of stearyl alcohol polyether, oleyl alcohol polyether, glyceryl stearate and cetyl polyethylene glycol, or a combination of several of them; the preservative is any one of phenoxyethanol, parabens and parahydroxyacetophenone, or a combination of several of them.

3. The cosmetic according to claim 2, characterized in that: It is composed of the following components in parts by weight: Water 60.0-65.0 parts; 0.5-1.5 parts of nucleic acid constituent substances; 4.0-7.0 parts of polydimethylsiloxane; 4.0-7.0 parts of cyclopentasiloxane; Glycerin 7.0-10.0 parts; 7.0-10.0 parts of 1,3-propylene glycol; 0.5-1.5 parts of hyaluronic acid; 1.0-1.5 parts of lauryl alcohol; 1.0-2.0 parts of polyacrylamide; Glyceryl stearate 2.0-3.0 parts; 0.2-2.0 parts of p-hydroxyacetophenone; 0.1-1.0 part of flavor; Preferably, the cosmetic is composed of the following components in parts by weight: 62.5 parts of water; 1.0 part of nucleic acid constituent material; 6.0 parts of polydimethylsiloxane; Cyclopentasiloxane 6.0 parts; Glycerin 9.0 parts; 9.0 parts of 1,3-propylene glycol; 1.0 part of hyaluronic acid; 1.3 parts of lauryl alcohol; 1.7 parts of polyacrylamide; 2.5 parts of glyceryl stearate; 0.6 parts of p-hydroxyacetophenone; 0.4 parts of flavor.

4. The cosmetic according to claim 1, characterized in that The nucleic acid constituent material is any one or a combination of guanosine, adenosine, cytidine and cytosine.

5. The cosmetic according to claim 4, characterized in that: When the nucleic acid constituent material is a composition of guanosine and adenosine, the weight ratio of guanosine to adenosine is (1:2) to (2:1); When the nucleic acid constituent material is a composition of guanosine and cytidine, the weight ratio of guanosine to cytidine is (1:2) to (2:1); When the nucleic acid composition material is a composition of adenosine and cytidine, the weight ratio of adenosine to cytidine is (1:2) to (2:1); When the nucleic acid constituent material is a composition of guanosine, adenosine and cytidine, the weight ratio of guanosine, adenosine and cytidine is (1-3): (1-2): (1-3); When the nucleic acid constituent material is a composition of guanosine, adenosine, cytidine and cytosine, the weight ratio of guanosine, adenosine, cytidine and cytosine is (1-4): (1-3): (1-2): 1; Preferably, When the nucleic acid constituent material is a combination of guanosine and adenosine, the weight ratio of guanosine to adenosine is 1:1; When the nucleic acid composition material is a combination of guanosine and cytidine, the weight ratio of guanosine to cytidine is 1:1; When the nucleic acid composition material is a composition of adenosine and cytidine, the weight ratio of adenosine to cytidine is 1:1; When the nucleic acid constituent material is a composition of guanosine, adenosine and cytidine, the weight ratio of guanosine, adenosine and cytidine is 1:1:1; When the nucleic acid constituent material is a composition of guanosine, adenosine, cytidine and cytosine, the weight ratio of guanosine, adenosine, cytidine and cytosine is 1:1:1:

1.

6. The method for preparing the cosmetics according to claim 1, characterized in that: The steps include: (1) Dissolving: mixing the water, moisturizing agent and nucleic acid constituent material in the above-mentioned weight proportions, stirring and heating for emulsification to obtain an aqueous phase; (2) mixing the oil phase: uniformly mixing the polydimethylsiloxane, cyclopentasiloxane, lauryl alcohol, emulsifier and polyacrylamide in the above-mentioned weight proportions to obtain an oil phase; (3) emulsification: mixing the oil phase obtained in step (2) and the water phase obtained in step (1) at a controlled temperature, and then emulsifying to obtain an emulsified mixture; (4) Aging: lowering the system temperature of the emulsified mixture obtained in step (3), adding the essence in the aforementioned weight proportion to the system; then continuing to cool to room temperature and aging to obtain the cosmetic; The preservative in the above-mentioned weight proportion can be added in the emulsification step of step (3), or can be added together with the flavor in step (4).

7. The preparation method according to claim 6, characterized in that: In step (1), the emulsification temperature is 70-85° C. and the emulsification time is 40-50 min; the stirring speed is 1000-1500 rpm.

8. The preparation method according to claim 6, characterized in that: In step (2), the mixing is carried out at 70-85° C.; the mixing requires stirring, the stirring speed is 800-1500 rpm, and the stirring time is 10-50 min.

9. The preparation method according to claim 6, characterized in that: In step (3), the temperature is controlled at 70-75°C; the emulsification temperature is 70-75°C and the emulsification time is 20-50 min; during the emulsification process, stirring is required at a stirring speed of 1000-1500 rpm; In step (4), the system temperature is lowered to 30-40° C.; and the aging time is 2-6 hours.

10. Use of the cosmetic according to any one of claims 1 to 5 in the preparation of cosmetics having moisturizing and / or anti-oxidation properties and / or wrinkle-reducing and / or sun-protection functions.

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