MUC1 mutation detection system and non-disease diagnosis purpose method

Abstract

The invention relates to the technical field of gene detection, in particular to an MUC1 mutation detection system and a non-disease diagnosis target method. The system comprises a collection module used for collecting peripheral blood of a to-be-detected person and extracting and breaking high molecular weight DNA, a Y-shaped pre-library preparation module used for conducting end repair and joint connection on fragmented DNA to construct a Y-shaped pre-library, and a targeted enrichment module used for conducting hybridization and targeted enrichment on target genes through a target area capture probe. The single-molecule real-time sequencing library construction module is used for carrying out three-generation sequencing linker connection to obtain a single-molecule real-time sequencing library, and the single-molecule real-time sequencing analysis module is used for carrying out single-molecule real-time sequencing to obtain sequencing data of a target area, and disassembling and analyzing the sequencing data of the target area to obtain a mutation type of MUC1; detection is carried out through three-generation sequencing, mutation setting is accurate, and the problem that an existing detection scheme is not practical is solved.

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