Manifold assembly, fluid system, and biochemical assay detection platform

Abstract

A manifold assembly, a fluid system, and a biochemical analysis detection platform. The manifold assembly includes: a manifold body (101) having one or more first external flow path interfaces (102-109), one or more first internal flow path interfaces (127-134), one or more second internal flow path interfaces (120, 123, 125, 126, 135-138), one or more second external flow path interfaces (118), and a plurality of internal flow paths (162-179), the plurality of internal flow paths including one or more first internal flow paths (162-169) and one or more second internal flow paths (170-179), each first external flow path interface (102-109) being in communication with at least one first internal flow path interface (127-134) through a corresponding first internal flow path (162-179), each second external flow path interface (118) being in communication with at least one second internal flow path interface (120, 123, 125, 126, 135-138) through a corresponding second internal flow path (170-179); and one or more internal flow path control valves (110-117), each internal flow path control valve (110-117) being connected between at least one first internal flow path interface (127-134) and at least one second internal flow path interface (120, 123, 125, 126 135-138) and being configured to control the on-off of the first internal flow path interface (127-134) and the second internal flow path interface (120, 123, 125, 126, 135 138) connected thereto.

Description

Technical Field

[0001] This disclosure relates to the field of fluid system technology, and in particular to a manifold assembly, a fluid system, and a biochemical analysis and detection platform. Background Technology

[0002] In related technologies, biochemical analysis, such as gene sequencing, involves switching between various reagents (or samples). To facilitate this switching, reversing devices (or reagent selection devices), such as rotary valves or slider valves, are typically used between the reagent kit (or sample cassette) and the slide. These reversing devices have multiple channels between themselves and the slide, leading to high reagent consumption and higher testing costs.

[0003] In related technologies, to address the issues of high reagent consumption and high testing costs, an integrated flow channel carrier has been proposed. However, the carrier is a consumable and needs to be replaced frequently. This integrated flow channel carrier relies on external active valve control devices. When replacing the carrier, the number of interfaces that need to be connected immediately is large, making sealing difficult. Furthermore, to ensure that all interfaces are well sealed, the manufacturing precision of the integrated flow channel carrier needs to be improved. Therefore, the manufacturing cost of this integrated flow channel carrier is high, the manufacturing difficulty is high, and the reliability is poor. Summary of the Invention

[0004] The purpose of this disclosure is to provide a manifold assembly, a fluid system, and a biochemical analysis and detection platform.

[0005] The first aspect of this disclosure provides a manifold assembly, comprising:

[0006] The manifold body includes one or more first external flow channel interfaces, one or more first internal flow channel interfaces, one or more second internal flow channel interfaces, one or more second external flow channel interfaces, and a plurality of internal flow channels. The plurality of internal flow channels includes one or more first internal flow channels and one or more second internal flow channels. Each first external flow channel interface is connected to at least one first internal flow channel interface through a corresponding first internal flow channel, and each second external flow channel interface is connected to at least one second internal flow channel interface through a corresponding second internal flow channel.

[0007] One or more internal flow channel control valves, each of which is connected between at least one first internal flow channel interface and at least one second internal flow channel interface, and is configured to control the on / off state of the first internal flow channel interface and the second internal flow channel interface to which it is connected.

[0008] In some embodiments of the manifold assembly,

[0009] At least two of the first internal flow channels are connected to the corresponding first external flow channel interface through another first internal flow channel; and / or

[0010] At least two of the second internal flow channels are connected to the corresponding second external flow channel interface through another second internal flow channel.

[0011] In some embodiments of the manifold assembly,

[0012] The internal flow channel control valve is a solenoid valve; and / or

[0013] The internal flow channel control valve is a reversing valve.

[0014] In some embodiments of the manifold assembly,

[0015] At least one of the internal flow channel control valves is provided corresponding to one of the first internal flow channel interfaces and one of the second internal flow channel interfaces; and / or

[0016] At least one of the internal flow channel control valves is provided corresponding to one first internal flow channel interface and two or more second internal flow channel interfaces; and / or

[0017] At least one of the internal flow channel control valves is provided corresponding to two or more first internal flow channel interfaces and one second internal flow channel interface; and / or

[0018] At least one of the internal flow channel control valves is provided corresponding to two or more first internal flow channel interfaces and two or more second internal interfaces.

[0019] In some embodiments of the manifold assembly,

[0020] The one or more internal flow channels include a third internal flow channel, which is connected to at least one second internal flow channel;

[0021] The manifold body includes a third external flow channel interface, which is connected to the third internal flow channel.

[0022] The manifold assembly includes a bypass control valve configured to control the opening and closing of the third internal flow passage connected thereto and the at least one second internal flow passage connected thereto.

[0023] In some embodiments of the manifold assembly, the bypass control valve includes three ports, two of which are connected to two second internal flow channels respectively, and the third port is connected to the third internal flow channel. The bypass control valve is configured to connect the two second internal flow channels and disconnect the third internal flow channel, or to connect one of the two second internal flow channels to the third internal flow channel and disconnect the other.

[0024] In some embodiments of the manifold assembly, the manifold body is a multi-manifold plate, wherein,

[0025] Multiple first external flow channel interfaces are arranged side by side on the same side of the multi-manifold plate and / or;

[0026] Multiple second external flow channel interfaces are arranged side-by-side on the same side of the multi-manifold plate; and / or

[0027] Multiple first internal flow channel interfaces and multiple external flow channel interfaces are arranged side-by-side on the same side of the multi-manifold plate; and / or

[0028] Multiple internal flow control valves are arranged side-by-side on the same side of the manifold.

[0029] A second aspect of this disclosure provides a fluid system, comprising:

[0030] The manifold assembly described in the first aspect of this disclosure;

[0031] A fluid storage device includes one or more reagent storage areas for storing reagents, the reagent storage areas being connected to a corresponding first external flow channel interface;

[0032] A carrier plate, including at least one flow cell, each flow cell being connected to a corresponding second external flow channel interface of the manifold assembly; and

[0033] One or more power sources, each power source being configured to drive the reagent in the reagent storage area to the corresponding flow cell.

[0034] In some embodiments of the fluid system, the fluid system further includes a reversing valve disposed between the fluid storage device and one or more first external flow channel interfaces of the manifold body, configured to selectively connect one of the reagent storage areas to one of the first external flow channel interfaces.

[0035] In some embodiments of the fluid system, the fluid system further includes a bypass line configured to connect to at least one of the plurality of internal flow channels to allow reagents entering the manifold body to flow into the bypass line.

[0036] In some embodiments of the fluid system, the slide includes two or more flow pools, and the fluid system includes two or more power sources corresponding to the two or more flow pools.

[0037] In some embodiments of the fluid system, the fluid system further includes a waste liquid storage module configured to receive waste liquid from the flow tank.

[0038] A third aspect of this disclosure provides a biochemical analysis and detection platform, which includes the manifold assembly described in the first aspect of this disclosure or the fluid system described in the second aspect of this disclosure.

[0039] In some embodiments of the biochemical analysis and detection platform, the biochemical analysis and detection platform includes a gene sequencer, which includes the manifold assembly or the fluid system. Based on the manifold assembly of this disclosure, the manifold body and multiple internal flow channel control valves form a manifold assembly. Compared with the related technologies of integrated flow channels for slides described in the background art, the flow channels for the slides are integrated into the manifold assembly, and the communication relationship between the multiple internal flow channels of the manifold body is controlled by the internal flow channel control valves. This limits the number of slide interfaces to the necessary number of interfaces matching the number of flow cells, which is beneficial to improving the sealing performance between the slide interfaces and the connected fluid elements. Furthermore, since the number of slide interfaces is small, there is no need to impose higher processing requirements on the slides, thereby helping to control the slide manufacturing cost and reduce manufacturing difficulty. The various flow channels and flow channel control components for supplying reagents to the slides are integrated into the manifold assembly, which does not require frequent replacement. This allows for the reduction of the amount of reagent in the flow channels while providing reagent supply flow channels that meet the detection requirements.

[0040] The fluid system and biochemical analysis platform disclosed herein have the advantages of the manifold components disclosed herein. Attached Figure Description

[0041] The accompanying drawings, which are included to provide a further understanding of this disclosure and form part of this disclosure, illustrate exemplary embodiments of the present disclosure and are used to explain the disclosure, but do not constitute an undue limitation of the disclosure. In the drawings:

[0042] Figure 1 This is a schematic diagram of the structure of a manifold assembly according to an embodiment of the present disclosure.

[0043] Figure 2A and Figure 2B for Figure 1 The diagram shows a cross-sectional view of the manifold body structure of the manifold assembly.

[0044] Figure 3 This is a schematic diagram of a fluid system according to an embodiment of the present disclosure.

[0045] Figure 4 This is a cross-sectional view of the manifold body of a manifold assembly according to another embodiment of the present disclosure.

[0046] Figure 5A and Figure 5B This is a cross-sectional view of the manifold body of a manifold assembly according to another embodiment of the present disclosure.

[0047] Figure 6This is a cross-sectional view of the manifold body of a manifold assembly according to another embodiment of the present disclosure.

[0048] Figure 7A and Figure 7B This is a cross-sectional view of the manifold body of a manifold assembly according to another embodiment of the present disclosure.

[0049] Figure 8 This is a schematic diagram of the structure of a fluid system according to another embodiment of the present disclosure. Detailed Implementation

[0050] The technical solutions of the embodiments of this disclosure will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this disclosure, and not all embodiments. The following description of at least one exemplary embodiment is merely illustrative and is in no way intended to limit this disclosure or its application or use. All other embodiments obtained by those skilled in the art based on the embodiments of this disclosure without creative effort are within the scope of protection of this disclosure.

[0051] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps set forth in these embodiments do not limit the scope of this disclosure. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following drawings denote similar items; therefore, once an item is defined in one drawing, it need not be further discussed in subsequent drawings.

[0052] like Figures 1 to 8 As shown, this disclosure provides a manifold assembly including a manifold body and one or more internal flow channel control valves.

[0053] The manifold body includes one or more first external flow channel interfaces, one or more first internal flow channel interfaces, one or more second internal flow channel interfaces, one or more second external flow channel interfaces, and multiple internal flow channels. The multiple internal flow channels include one or more first internal flow channels and one or more second internal flow channels. Each first external flow channel interface is connected to at least one first internal flow channel interface through a corresponding first internal flow channel, and each second external flow channel interface is connected to at least one second internal flow channel interface through a corresponding second internal flow channel.

[0054] Each internal flow channel control valve is connected between at least one first internal flow channel interface and at least one second internal flow channel interface, and is configured to control the on / off state of the first internal flow channel interface and the second internal flow channel interface to which it is connected.

[0055] In this embodiment, the manifold body and multiple internal flow channel control valves form a manifold assembly. Compared to the related technologies of integrated flow channel carriers described in the background art, the flow channels for the carrier are integrated within the manifold assembly, and the communication relationship between the multiple internal flow channels of the manifold body is controlled by the internal flow channel control valves. This limits the number of carrier interfaces to the necessary number of interfaces matching the number of flow cells, which is beneficial to improving the sealing performance between the carrier 140 interfaces and the connected fluid elements. Furthermore, since the number of carrier interfaces is small, there is no need to impose higher processing requirements on the carrier, thereby helping to control carrier manufacturing costs and reduce manufacturing difficulty. The various flow channels and flow channel control components that supply reagents to the carrier are integrated within the manifold assembly, which does not require frequent replacement. This allows for the reduction of reagent volume within the flow channels while providing reagent supply channels that meet detection requirements.

[0056] In some embodiments of the manifold assembly, at least two first internal flow channels are connected to a corresponding first external flow channel interface through another first internal flow channel, such as... Figures 1 to 6 As shown; and / or at least two second internal flow channels are connected to the corresponding second external flow channel interface through another second internal flow channel, such as Figure 7A , Figure 7B and Figure 8 As shown. For example: Figures 1 to 6 As shown, at least two first internal flow channels are connected to the corresponding first external flow channel interface through another first internal flow channel; as Figure 7A , Figure 7B and Figure 8 As shown, at least two second internal flow channels are connected to the corresponding second external flow channel interface through another second internal flow channel.

[0057] The above internal flow channel arrangement utilizes a tree-like branch design, which helps reduce the number of sealing surfaces between the manifold body 101 and external fluid components, thereby improving reliability.

[0058] In some embodiments of the manifold assembly, the internal flow control valve is a solenoid valve; and / or the internal flow control valve is a directional valve. For example: in Figures 1 to 4 , Figure 6 In the illustrated embodiment, the internal flow control valve is a two-position, two-way solenoid valve; Figure 5A , Figure 5B , Figure 7A , Figure 7B and Figure 8 In the embodiment shown, the internal flow control valve is a two-position three-way solenoid valve.

[0059] Using a directional valve as the internal flow channel control valve facilitates accurate valve position switching and enables the on / off switching of corresponding interfaces. Using a solenoid valve as the internal flow channel control valve allows for miniaturization, high integration of fluid control functions, and strong scalability in the number of interfaces. It also helps reduce the weight and height of the manifold assembly, lowering the requirements for the load-bearing capacity and motion control precision of the carrier's motion platform.

[0060] In some embodiments of the manifold assembly, at least one internal flow channel control valve is configured corresponding to one first internal flow channel interface and one second internal flow channel interface; and / or at least one internal flow channel control valve is configured corresponding to one first internal flow channel interface and two or more second internal flow channel interfaces; and / or at least one internal flow channel control valve is configured corresponding to two or more first internal flow channel interfaces and one second internal flow channel interface; and / or at least one internal flow channel control valve is configured corresponding to two or more first internal flow channel interfaces and two or more second internal interfaces. For example: Figures 1 to 4 , Figure 6 As shown, each internal flow channel control valve is configured with a corresponding first internal flow channel interface and a second internal flow channel interface; for example... Figure 5A and Figure 5B As shown, each internal flow channel control valve is configured corresponding to two first internal flow channel interfaces and one second internal flow channel interface; for example... Figure 7A and Figure 7B As shown, each internal flow channel control valve is correspondingly configured with one first internal flow channel interface and two second internal flow channel interfaces. In embodiments not shown, one internal flow channel control valve may be connected to more first internal flow channel interfaces and second internal flow channel interfaces.

[0061] like Figure 6 As shown, in some embodiments of the manifold assembly, one or more internal flow channels further include a third internal flow channel connected to at least one second internal flow channel; one or more internal flow channels further include a third external flow channel interface connected to the third internal flow channel; the manifold assembly further includes a bypass control valve configured to control the on / off state of the third internal flow channel.

[0062] The above-mentioned third internal flow channel, third external flow channel interface and bypass control valve are designed to facilitate the formation of a bypass flow path in the fluid system where the manifold assembly is located, thereby increasing the operational flexibility of the fluid system.

[0063] like Figure 6As shown, in some embodiments of the manifold assembly, the bypass control valve includes three ports, two of which are connected to two second internal flow channels respectively, and the third port is connected to a third internal flow channel. The bypass control valve is configured to connect the two second internal flow channels while disconnecting the third internal flow channel, or to connect one of the two second internal flow channels to the third internal flow channel while disconnecting the other. Figure 6 In the illustrated embodiment, the bypass control valve is, for example, a two-position three-way solenoid valve.

[0064] like Figures 1 to 8 As shown, in some embodiments of the manifold assembly, the manifold body is a multi-manifold plate, and multiple first external flow channel interfaces are arranged side by side on the same side of the multi-manifold plate and / or multiple second external flow channel interfaces are arranged side by side on the same side of the multi-manifold plate; and / or multiple first internal flow channel interfaces and multiple external flow channel interfaces are arranged side by side on the same side of the multi-manifold plate; and / or multiple internal flow channel control valves are arranged side by side on the same side of the multi-manifold plate.

[0065] like Figure 3 and Figure 8 As shown, this disclosure also provides a fluid system. The fluid system includes a fluid storage device 153, a manifold assembly and a carrier 140 according to embodiments of this disclosure, and one or more power sources 143. The fluid storage device 153 includes one or more reagent storage areas for storing reagents, each reagent storage area being connected to a corresponding first external flow channel interface. The carrier 140 includes at least one flow cell 140A, each flow cell 140A being connected to a corresponding second external flow channel interface of the manifold assembly. Each power source 143 is configured to drive the reagents from the reagent storage areas to the corresponding flow cells 140A.

[0066] The fluid system of this disclosure has the same advantages as the manifold assembly of this disclosure.

[0067] like Figure 3 and Figure 8 As shown, in some embodiments of the fluid system, the fluid system further includes a reversing valve 151, which is disposed between the fluid storage device 153 and one or more first external flow channel interfaces of the manifold 101 and is configured to selectively connect a reagent storage area to a first external flow channel interface.

[0068] like Figure 3 and Figure 8 As shown, in some embodiments of the fluid system, the fluid system further includes a bypass line 146, which is configured to connect to at least one of a plurality of internal flow channels to allow reagents entering the manifold body 101 to flow into the bypass line 146.

[0069] like Figure 3 and Figure 8As shown, in some embodiments of the fluid system, the carrier 140 includes two or more flow pools 140A, and the fluid system includes two or more power sources 143 corresponding to the two or more flow pools 140A.

[0070] like Figure 3 and Figure 8 As shown, in some embodiments of the fluid system, the fluid system further includes a waste liquid storage module 145, which is configured to receive waste liquid and bubbles in the flow pool 140A and the flow path.

[0071] This disclosure provides a biochemical analysis and detection platform, including a manifold assembly or a fluid system as described in this disclosure. The biochemical analysis and detection platform may include, for example, a gene sequencer, which may include the manifold assembly or the fluid system as described in this disclosure.

[0072] The biochemical analysis and detection platform of this disclosure has the same advantages as the manifold assembly and fluid system of this disclosure.

[0073] The following combination Figures 1 to 8 The manifold assembly and fluid system according to embodiments of the present disclosure will be described in more detail.

[0074] like Figures 1 to 3 As shown, the manifold assembly 100 is an integrated solenoid valve manifold assembly, including a manifold body 101 and one or more internal flow control valves 110 to 117.

[0075] The manifold body 101 is used to integrate multiple internal flow channels. If necessary, it can also integrate required functional devices, such as sensors. The manifold body 101 has multiple internal flow channels and multiple interfaces. In this embodiment, the manifold body 101 is implemented as a multi-manifold board with multiple internal flow channels and multiple interfaces. The multiple interfaces include one or more first external flow channel interfaces, one or more first internal flow channel interfaces, one or more second internal flow channel interfaces, and one or more second external flow channel interfaces. The multiple internal flow channels include one or more first internal flow channels and one or more second internal flow channels. Each first external flow channel interface is connected to at least one first internal flow channel interface through a corresponding first internal flow channel, and each second external flow channel interface is connected to at least one second internal flow channel interface through a corresponding second internal flow channel.

[0076] The shape of each internal flow channel of the manifold body 101 can be various. For example, the cross section perpendicular to the fluid flow direction can be a circular cross section, a D-shaped cross section, a square cross section, a trapezoidal cross section, etc. Along the fluid flow direction, the internal flow channel can be a constant cross section flow channel or a variable cross section flow channel. For example, a horn-shaped flow channel can be a variable cross section flow channel.

[0077] like Figures 1 to 3 In the illustrated embodiment, one or more first external flow channel interfaces include external flow channel interfaces 102 to 109; one or more first internal flow channel interfaces include internal flow channel interfaces 127 to 134; one or more second internal flow channel interfaces include internal flow channel interfaces 123, 125, 126, and 135 to 138; one or more second external flow channel interfaces include external flow channel interface 118; one or more first internal flow channels include internal flow channels 162 to 169; one or more second internal flow channels include internal flow channels 170 to 179; and one or more internal flow channel control valves include internal flow channel control valves 110 to 117. The multiple internal flow channels can be arranged in one layer or multiple layers, i.e., the multiple flow channels can be on one plane, two planes, or more planes. Corresponding to the arrangement of the multiple internal flow channels, the multiple interfaces can also be arranged in one layer or multiple layers.

[0078] Among the multiple internal flow channels, internal flow channel 179 is a common flow channel that is directly connected to the external flow channel interface 118. Internal flow channels 170 to 177 are all connected to internal flow channel 179, and internal flow channels 170 to 179 form a tree-like branch structure.

[0079] Each external flow channel interface 102-109 is used to connect the manifold body 101 to external fluid devices of the manifold assembly 100. For example, the external flow channel interfaces 102-109 can be connected to reagent kits, sample boxes, or other fluid devices, such as rotary valves, solenoid valves, etc. The external flow channel interfaces 102-109 can serve as both fluid inlets and outlets.

[0080] The external flow channel interface 118 is used to connect the internal flow channel 179 to the flow pool 140A of the carrier 140. There can be one or more external flow channel interfaces 118. Figures 1 to 3 The number of the second external flow channel interface 118 is 1.

[0081] Each of the internal flow channel interfaces 127 to 134 is used to connect to one port of an internal flow channel control valve to achieve connection with the external flow channel of the manifold assembly 100; each of the internal flow channel interfaces 120, 123, 125, 126, 135 to 138 is used to connect to the other port of an internal flow channel control valve to achieve communication with the internal flow channel 179 and the external flow channel interface 118 of the manifold body 101, thereby achieving connection with the flow pool 140A of the carrier 140.

[0082] Internal flow control valves 110 to 117 are used to switch (or disconnect) the internal flow channels they are connected to. In this embodiment, internal flow control valves 110 to 117 are all two-position two-way solenoid directional valves.

[0083] like Figures 1 to 3 As shown, multiple external flow channel interfaces 102 to 109 are connected to multiple internal flow channel interfaces 134, 133, 132, 131, 130, 129, 128, and 127 respectively through internal flow channels 162 to 169; one port of internal flow channel control valves 110 to 117 is connected to internal flow channel interfaces 134, 133, 132, 131, 130, 129, 128, and 127 respectively; another port of internal flow channel control valves 110 to 117 is connected to internal flow channel interfaces 135 to 138, 120, 123, 125, and 126 respectively; multiple internal flow channel interfaces 135 to 138, 120, 123, 125, and 126 are connected to internal flow channel 179 through internal flow channels 170 to 177; internal flow channel 179 is connected to external flow channel interface 118.

[0084] Internal flow channel 179 is a common flow channel through which two or more reagents flow. In this embodiment, internal flow channels 170 to 177 connected to internal flow channel interfaces 120, 123, 125, 126, and 135-138 can directly converge or branch into internal flow channel 179. For example, pipes connected to internal flow channel interfaces 120, 123, 125, 126, and 135-138 can converge in pairs into one flow channel, and then converge into internal flow channel 179. In this embodiment, internal flow channels 175 connected to internal flow channel interface 123 and internal flow channel 176 connected to internal flow channel interface 125 first converge into internal flow channel 178, and internal flow channel 178 then connects to internal flow channel 179. Other internal flow channels 170 to 174 and 177 are directly connected to internal flow channel 179.

[0085] In an embodiment not shown, the internal channels connected to the internal channel interfaces 120, 123, 125, 126, 135-138 can converge into a single point in the internal channel 179.

[0086] like Figures 1 to 3 As shown, the main operating process of a fluid system with the aforementioned manifold assembly includes:

[0087] 1. Flowchart of reagent fluid flowing into slide 140

[0088] When the internal flow channel control valve 117 is in the first position and is in the open state, the internal flow channel interface 126 is connected to the internal flow channel interface 127. The fluid (reagent / sample / gas) enters the manifold body 101 from the first external flow channel interface 109, flows through the internal flow channel 169 and the internal flow channel control valve 117, and then flows out from the external flow channel interface 118 through the internal flow channel interface 126, the internal flow channel 177 and the internal flow channel 179.

[0089] When the internal flow channel control valve 116 is in the first position and is in the open state, the internal flow channel interface 125 is connected to the internal flow channel interface 128. The fluid (reagent / sample / gas) enters the manifold body 101 along the internal flow channel interface 108, flows through the internal flow channel 168 and the internal flow channel control valve 116, and then flows out from the external flow channel interface 118 through the internal flow channel interface 125 and the internal flow channels 176, 178 and 179.

[0090] 2. Reagent flow process in bypass

[0091] When the internal flow channel control valve 116 and internal flow channel control valve 111 are in the first position and are in the open state, the internal flow channel interface 125 is connected to the internal flow channel interface 128, and the internal flow channel interface 136 is connected to the internal flow channel interface 133. The fluid (reagent / sample / gas) enters the manifold body 101 along the first external flow channel interface 108, flows through the internal flow channel 168, internal flow channel control valve 116, internal flow channel 176, internal flow channel 178, internal flow channel 179, internal flow channel 171, and internal flow channel control valve 111 in sequence, and flows out of the manifold body 101 from the external flow channel interface 103.

[0092] The above description only covers the working process of some internal flow channels, some interfaces, and some internal flow channel control valves. The working processes of the remaining internal flow channels, interfaces, and internal flow channel control valves not described can be referred to the preceding description.

[0093] Alternatively, the fluid can also flow in the opposite direction along the aforementioned path under the action of the driving source.

[0094] As can be seen from the manifold assembly 100 in the above embodiments, the manifold assembly 100 of this disclosure adopts an integrated flow channel, which can reduce the common volume, reduce reagent consumption, and reduce detection (such as gene sequencing) costs; the number of interfaces of the slide 140 in the fluid system where the manifold assembly 100 is located can be limited to the necessary number of interfaces matching the number of flow cells, which is beneficial to improve the sealing performance of the interfaces of the slide 140 and the connected fluid elements, and since the number of slide interfaces is small, there is no need to impose higher processing requirements on the slide, thereby helping to control the slide manufacturing cost and reduce manufacturing difficulty; the adoption of an internal flow channel control valve can reduce the weight and height of the slide 140 motion platform, reduce the requirements for the load-bearing capacity and motion control accuracy of the slide 140, and achieve a reliable sealed connection.

[0095] Figure 3 For having Figures 1 to 3 The fluid system of the manifold assembly 100 shown. For example... Figure 3As shown, the fluid system includes a power source 143, a waste liquid storage module 145, a fluid storage device 153, a pipette assembly, a fluid piping assembly, a manifold assembly, and a reversing valve 151. The fluid piping assembly includes pipes 141, 144, and 146. The pipette assembly includes multiple reagent pipes, such as reagent pipe 152.

[0096] The manifold assembly includes a manifold body 101 and a plurality of internal flow control valves 110 to 117. For details, please refer to the preceding description of the manifold assembly 100.

[0097] Figure 3 In the embodiment shown, the carrier 140 has a flow cell 140A.

[0098] Power source 143 is used to move samples or reagents within the fluid system. Power source 143 can generate pressure of a certain form, including negative or positive pressure, depending on the direction required for fluid transport. Power source 143 has multiple fluid interfaces to facilitate the transport of different fluids. For example, power source 143 can be connected to pipe 141 or pipe 146 to transport samples, reagents, or cleaning solutions, and connected to pipe 144 to discharge waste liquid. Power source 143 may include hydraulic units such as peristaltic pumps, plunger pumps, syringe pumps, gear pumps, and diaphragm pumps, or pneumatic units such as vacuum pumps, diaphragm pumps, air compressors, and air tanks with a certain positive or negative pressure. Power source 143 may include a directional valve 142, which can be a solenoid valve, rotary valve, pneumatic directional valve, electro-hydraulic directional valve, manual directional valve, piezoelectric valve, pinch valve, rotary valve, or rotary shear valve.

[0099] The waste liquid storage module 145 has one or more interfaces for storing or discharging all or part of the waste liquid from the fluid system. When the fluid system belongs to an instrument, such as a gene sequencer, the waste liquid storage module 145 can be located inside or outside the instrument body, or it can be directly discharged to a laboratory waste liquid treatment system via its interfaces. The waste liquid storage module 145 may or may not have a container, or it may include one or more containers. Depending on functional requirements, the waste liquid storage module 145 may also include liquid detection sensors, air purification devices, or secondary spill prevention devices.

[0100] The fluid storage device 153 is used to store liquids such as reagents, samples, and cleaning solutions required by the fluid system during biochemical detection (such as gene sequencing). Each type of liquid can correspond to a reagent storage area. In this embodiment, the fluid storage device 153 includes multiple reagent bottles, and the receiving portion of each reagent bottle constitutes a reagent storage area.

[0101] The pipette assembly is used to draw liquid from the fluid storage device 153 and deliver it externally. The pipette assembly may include a reagent tube, a reagent needle, or a manifold. The pipette assembly may also include a filter, a bubble sensor, etc.

[0102] Fluid piping assemblies are used to connect fluid devices and transport fluids. Fluid piping assemblies may include detection devices such as pressure sensors, flow sensors, and bubble sensors mounted on the pipes to monitor the state of the fluid system.

[0103] The reversing valve 151 is used to switch the connection relationships between different pipette components and different first external flow channel interfaces of the manifold body 101, so that samples or reagents are transported along a set path. For example, the internal flow channel control valve 117 has two ports, one of which can be connected to the carrier 140, and the other port can be connected to the fluid storage device 153, or it can be connected to the fluid storage device 153 via other fluid devices. Thus, the reversing valve 151 can control whether the fluid storage device 153 is connected to the carrier 140. The reversing valve 151 can be a solenoid valve, rotary valve, pneumatic reversing valve, electro-hydraulic reversing valve, manual reversing valve, piezoelectric valve, pinch valve, rotary valve, swivel valve, etc. In this embodiment, the reversing valve 151 is a rotary valve. The rotary valve has eight ports, each of which is connected to a reagent bottle.

[0104] Figures 1 to 3 As shown, the main working process of the above fluid system includes:

[0105] 1. The process of reagent flowing into slide 140

[0106] The reversing valve 151 switches to the 8th position, the internal flow channel control valve 117 is in the first position, and the reversing valve 142 of the power source 143 switches to be connected to the pipeline 141. The power source 143 generates negative pressure, and the reagent flows from the corresponding reagent bottle of the fluid storage device 153 along the reagent pipeline 152, reversing valve 151, reagent pipeline 150, internal flow channel 169, internal flow channel control valve 117, internal flow channel 177, and internal flow channel 179 from the external flow channel interface 118 into the carrier 140.

[0107] 2. The process of reagent flowing into the bypass

[0108] The reversing valve 151 is switched to the 8th position, the internal flow control valves 117 and 111 are in the first position, and the reversing valve 142 of the power source 143 is switched to be connected to the pipeline 146. The power source 143 generates negative pressure, and the reagent is drawn from the corresponding reagent bottle of the fluid storage device 153 through the reagent pipeline 152 of the pipette assembly, the reversing valve 151, the reagent pipeline 150, the internal flow channel 169, the internal flow control valve 117, the internal flow channel 177, the internal flow channel 179, the internal flow channel 171, and the internal flow control valve 111 from the external flow channel interface 103 into the bypass pipeline 146.

[0109] 3. The process of reagent discharge

[0110] The reversing valve 142 of the power source 143 switches to connect with the pipeline 144, and the power source 143 generates positive pressure to discharge the reagent into the waste liquid storage module 145.

[0111] The above description illustrates the working process of the fluid system in this embodiment of the disclosure only by referring to the working process corresponding to a portion of the internal flow channels, a portion of the interfaces, and a portion of the internal flow channel control valves of the manifold assembly 100. The working processes of the fluid system related to the remaining internal flow channels, interfaces, and internal flow channel control valves of the manifold assembly 100, which are not described above, can be referred to the preceding description. The fluid system of this embodiment of the disclosure has the same advantages as the manifold assembly 100 of this embodiment of the disclosure. Figure 4 This is a schematic diagram of the manifold body of a manifold assembly according to another embodiment of this disclosure. The following description only illustrates this embodiment and... Figures 1 to 3 The differences between the manifold assembly 100 in the illustrated embodiment are as follows. Figure 4 Some of the Figures 1 to 3 The same structures shown in the embodiments are not labeled with reference numerals; the reference numerals for these structures can be found by referring to... Figures 1 to 3 The corresponding reference numerals of the illustrated embodiments.

[0112] like Figure 4 As shown, in this embodiment, there are two second external flow channel interfaces, including external flow channel interface 118 and external flow channel interface 202. External flow channel interface 118 and external flow channel interface 202 are connected to the ends of internal flow channel 179 through internal flow channel 201 and internal flow channel 203, respectively. That is, internal flow channel 179, which serves as a common flow channel, branches to form two second internal flow channels, namely internal flow channel 201 and internal flow channel 203, and the ends of internal flow channel 201 and internal flow channel 203 away from internal flow channel 179 are respectively connected to two external flow channel interfaces 118 and external flow channel interface 202, which serve as second external flow channel interfaces, to adapt to carriers 140 with different channels. Figure 4The manifold assembly of the illustrated embodiment can be adapted to a two-channel carrier 140. The two-channel carrier 140 includes two flow pools 140A arranged side by side, with fluid flowing into and out of the two flow pools 140A respectively.

[0113] in addition, Figure 4 In the illustrated embodiment, the internal flow channels 175 to 178, which serve as the second internal flow channels, are... Figures 1 to 3 The internal flow channels 175 to 178 in the illustrated embodiments are connected in different ways. Figure 4 In the illustrated embodiment, internal flow channels 175 to 177 are all connected to internal flow channel 178, and internal flow channels are connected to internal flow channel 179. That is, three second internal flow channels (internal flow channels 175 to 177) first converge into another second internal flow channel (internal flow channel 178) and then merge into the second internal flow channel (internal flow channel 179) which serves as a common flow channel. The remaining internal flow channels 170 to 174, which serve as second internal flow channels, each merge into internal flow channel 179 individually.

[0114] Figure 4 For any parts of the manifold assembly and fluid system not described in the corresponding embodiments, please refer to the relevant descriptions in the foregoing embodiments.

[0115] Figure 5A and Figure 5B This is a schematic diagram of the manifold body of a manifold assembly according to another embodiment of this disclosure. The following description only illustrates this embodiment and... Figures 1 to 3 The differences between the manifold assembly 100 in the illustrated embodiment are as follows. Figure 5A and Figure 5B Some of the Figures 1 to 3 The same structures shown in the embodiments are not labeled with reference numerals; the reference numerals for these structures can be found by referring to... Figures 1 to 3 The corresponding reference numerals of the illustrated embodiments.

[0116] like Figure 5A and Figure 5B As shown, in this embodiment of the disclosure, compared to Figures 1 to 3 In the embodiment shown, in the manifold assembly, one or more first external flow channel interfaces further include external flow channel interfaces 212 to 219, one or more first internal flow channel interfaces further include internal flow channel interfaces 222 to 229, and one or more first internal flow channels further include internal flow channels 232 to 239.

[0117] In this embodiment of the disclosure, instead of Figures 1 to 3The internal flow control valves 110 to 117 of the illustrated embodiment, which have two ports, are internal flow control valves with three ports. In this embodiment, the internal flow control valve is, for example, a two-position three-way solenoid valve. Of the three ports of each internal flow control valve, the first port is connected to a second internal flow port, and the other two ports are each connected to a first internal flow port. Each internal flow control valve is configured such that its connected second internal flow port can selectively connect to one of its two connected first internal flow ports, while disconnecting from the other. In this embodiment, one port of each of the eight internal flow control valves is sequentially connected to internal flow ports 135 to 138, 120, 123, 125, and 126; one of the other two ports of the eight internal flow control valves is sequentially connected to internal flow interfaces 222 to 229; and the other of the other two ports of the eight internal flow control valves is sequentially connected to internal flow interfaces 134, 133, 132, 131, 130, 129, 128, and 127.

[0118] In this embodiment, when the internal flow control valves connecting internal flow interfaces 228, 128, and 125 and the internal flow control valves connecting internal flow interfaces 229, 127, and 126 are all in the first position, internal flow interfaces 128 and 125 are connected while internal flow interface 228 is disconnected, and internal flow interfaces 127 and 126 are connected while internal flow interface 229 is disconnected; when the internal flow control valves connecting internal flow interfaces 228, 128, and 125 and the internal flow control valves connecting internal flow interfaces 229, 127, and 126 are all in the second position, internal flow interfaces 228 and 125 are connected while internal flow interface 128 is disconnected, and internal flow interfaces 229 and 126 are connected while internal flow interface 127 is disconnected.

[0119] The control relationships of other internal flow channel control valves can be referenced from the internal flow channel control valves connected to internal flow channel interfaces 228, 128 and 125 and the internal flow channel control valves connected to internal flow channel interfaces 229, 127 and 126.

[0120] Figure 5A and Figure 5B For any parts of the manifold assembly and fluid system not described in the corresponding embodiments, please refer to the relevant descriptions in the foregoing embodiments.

[0121] Figure 6 This is a schematic diagram of the manifold body of a manifold assembly according to another embodiment of this disclosure. The following description only illustrates this embodiment and... Figures 1 to 3 The differences between the manifold assembly 100 in the illustrated embodiment are as follows. Figure 6 Some of the Figures 1 to 3 The same structures shown in the embodiments are not labeled with reference numerals; the reference numerals for these structures can be found by referring to... Figures 1 to 3 The corresponding reference numerals of the illustrated embodiments.

[0122] like Figure 6 As shown, compared to Figures 1 to 3 Regarding the manifold assembly of the illustrated embodiment, in the manifold assembly of this disclosure embodiment, one or more internal flow channels further include a third internal flow channel. In this embodiment, the third internal flow channel is internal flow channel 244. Internal flow channel 244 is connected to at least one second internal flow channel; the one or more internal flow channels also include a third external flow channel interface. The third external flow channel interface is external flow channel interface 245. External flow channel interface 245 is connected to internal flow channel 244. The manifold assembly also includes a bypass control valve (not shown), which is configured to control the on / off state of the internal flow channel 244 connected thereto and at least one second internal flow channel connected thereto.

[0123] The bypass control valve includes three ports. Two of the three ports are connected to internal flow channels 179 and 241, which serve as second internal flow channels, respectively, and the third port is connected to internal flow channel 244, which serves as a third internal flow channel. The bypass control valve is configured to connect internal flow channels 179 and 241 while disconnecting internal flow channel 244, or to connect one of internal flow channels 179 and 241 to internal flow channel 244 while disconnecting the other. Figure 6 In the illustrated embodiment, the bypass control valve is, for example, a two-position three-way solenoid valve.

[0124] like Figure 6 As shown, one or more interfaces further include internal flow channel interfaces 240, 242, and 243. One or more second internal flow channels further include an internal flow channel 241. An internal flow channel 244 is connected to an internal flow channel interface 240. Both ends of the internal flow channel 241 are connected to an internal flow channel interface 242 and an external flow channel interface 118, respectively. An internal flow channel 179 is connected to an internal flow channel interface 243. The three ports of the bypass control valve are connected to internal flow channel interfaces 240, 241, and 243, respectively, thereby achieving connection to internal flow channels 244, 179, and 241, respectively.

[0125] Figure 6 The manifold assembly of the illustrated embodiment can realize reagent bypass function. Of course, samples or reagents can also be loaded from the third external flow channel interface through the third external flow channel interface, the third internal flow channel, and the bypass control valve.

[0126] Figure 6 For any parts of the manifold assembly and fluid system not described in the corresponding embodiments, please refer to the relevant descriptions in the foregoing embodiments.

[0127] Figure 7A and Figure 7B This is a schematic diagram of the manifold body of a manifold assembly according to another embodiment of the present disclosure. Figure 8This is a schematic diagram of the structure of a fluid system according to another embodiment of the present disclosure. Figure 8 The fluid system shown employs Figure 7A and Figure 7B The manifold assembly of the embodiment shown. Figures 7A to 8 The manifold body of the manifold assembly in the illustrated embodiment and Figures 1 to 3 The manifold body of the illustrated embodiment differs from that of the other embodiment. Figures 1 to 3 The manifold body of the illustrated embodiment includes multiple first external flow channel inlets and one second external flow channel interface, while Figures 7A to 8 The manifold body of the illustrated embodiment includes a first external flow channel inlet and a plurality of second external flow channel interfaces.

[0128] like Figure 7A , Figure 7B and Figure 8 As shown, the manifold assembly is an integrated solenoid valve manifold assembly, including a manifold body and multiple internal flow control valves.

[0129] The manifold body has multiple internal flow channels and multiple interfaces. In this embodiment, the manifold body is implemented as a multi-manifold plate with multiple internal flow channels and multiple interfaces. The multiple interfaces include one or more first external flow channel interfaces, one or more first internal flow channel interfaces, one or more second internal flow channel interfaces, and one or more second external flow channel interfaces. The multiple internal flow channels include one or more first internal flow channels and one or more second internal flow channels. Each first external flow channel interface is connected to at least one first internal flow channel interface through a corresponding first internal flow channel, and each second external flow channel interface is connected to at least one second internal flow channel interface through a corresponding second internal flow channel.

[0130] like Figure 7A , Figure 7B and Figure 8 In the illustrated embodiment, one or more first external flow channel interfaces include external flow channel interfaces 301 and 341 to 348; one or more first internal flow channel interfaces include internal flow channel interfaces 311 to 318 and 321 to 328; one or more second internal flow channel interfaces include internal flow channel interfaces 331 to 338; one or more second external flow channel interfaces include external flow channel interfaces 351 to 358; one or more first internal flow channels include internal flow channels 361 to 367, 371 to 378, and 381 to 388; one or more second internal flow channels include internal flow channels 391 to 398; and one or more internal flow channel control valves include eight internal flow channel control valves 302 to 309. The multiple internal flow channels can be arranged in one layer or multiple layers, i.e., the multiple flow channels can be on one plane, two planes, or more planes. Correspondingly, the multiple interfaces can also be arranged in one layer or multiple layers.

[0131] like Figure 7A , Figure 7B and Figure 8 In the illustrated embodiment, among the multiple internal flow channels, internal flow channels 361 to 367 and 371 to 378, which serve as the first internal flow channels, form a tree-like branching structure. For example... Figure 7A , Figure 7B and Figure 8 As shown, internal flow channel 361 is connected to external flow channel interface 301. The end of internal flow channel 361 furthest from external flow channel interface 301 branches into internal flow channels 362 and 363. Internal flow channel 362 branches into internal flow channels 364 and 365. Internal flow channel 363 branches into internal flow channels 366 and 367. Internal flow channel 364 branches into internal flow channels 371 and 372. Internal flow channel 365 branches into internal flow channels 373 and 374. Internal flow channel 366 branches into internal flow channels 375 and 376. Internal flow channel 367 branches into internal flow channels 377 and 378. Internal flow channels 371 to 378 are sequentially connected to internal flow channel interfaces 311 to 318, which serve as the first internal flow channel interface.

[0132] like Figure 7A , Figure 7B and Figure 8 In the illustrated embodiment, external flow channel interface 301 is used to connect the manifold body to external fluid devices, such as reagent kits, sample cassettes, or other fluid devices like rotary valves or solenoid valves. External flow channel interface 301 can serve as both a fluid inlet and outlet. External flow channel interfaces 341 to 348 can be connected to external reagent kits or sample cassettes. External flow channel interfaces 351 to 358 are used to connect to the flow cell 140A of the slide 140.

[0133] like Figure 7A , Figure 7B and Figure 8 As shown, multiple external flow channel interfaces 341 to 348 are connected to multiple internal flow channel interfaces 331 to 338 through internal flow channels 381 to 388, respectively; multiple external flow channel interfaces 351 to 358 are connected to multiple internal flow channel interfaces 321 to 328 through internal flow channels 391 to 398, respectively.

[0134] Internal flow control valves are used to reverse (or disconnect) the internal flow channels to which they are connected.

[0135] In this embodiment, each of the internal flow control valves 302 to 309 has three ports. For example, the internal flow control valves 302 to 309 are two-position three-way solenoid valves. One port of each internal flow control valve 302 to 309 is connected to a second internal flow port, and the other two ports are each connected to a first internal flow port. Each internal flow control valve 302 to 309 is configured such that one of its connected second internal flow ports can selectively connect to one of its connected two first internal flow ports, while the other is disconnected. In this embodiment, one port of each of the eight internal flow control valves 302 to 309 is sequentially connected to internal flow ports 321 to 328; one of the other two ports of each of the eight internal flow control valves 302 to 309 is sequentially connected to internal flow interfaces 311 to 318, and the other of the other two ports of each of the eight internal flow control valves is sequentially connected to internal flow interfaces 331 to 338.

[0136] like Figure 8 As shown, the fluid system includes multiple power sources 143, a waste liquid storage module 145, a fluid storage device 153, a pipette assembly, a fluid piping assembly, a manifold assembly, and a reversing valve 151. The fluid piping assembly includes pipes 141 and 144. The pipette assembly includes multiple reagent pipes, such as reagent pipe 152.

[0137] The manifold assembly includes a manifold body and multiple internal flow control valves 302 to 309. For details, please refer to the previous section... Figures 7A to 8 Description of the manifold assembly shown.

[0138] Figure 8 In the fluid system of the illustrated embodiment, the slide 140 has multiple flow cells 140A. Flow cells 140A are the sites for biochemical reactions during sequencing. Each flow cell 140A can be exactly the same size or vary in size. All flow cells 140A can be combined into a single inlet or outlet, or some flow cells 140A can be combined into a single inlet or outlet while others have independent outlets. In this embodiment, the multiple flow cells 140A are independent of each other. The multiple flow cells 140A are respectively connected to the external flow channel interfaces 351 to 358 of the manifold body.

[0139] Power source 143 is used to move samples or reagents within the fluid system. Power source 143 can generate pressure of a certain form, including negative or positive pressure, depending on the direction required for fluid transport. Power source 143 has multiple fluid interfaces to facilitate the transport of different fluids. For example, power source 143 connected to pipe 141 can transport samples, reagents, or cleaning solutions, while connected to pipe 144 can discharge waste liquid. Power source 143 may include hydraulic units such as peristaltic pumps, plunger pumps, syringe pumps, gear pumps, and diaphragm pumps, or pneumatic units such as vacuum pumps, diaphragm pumps, air compressors, and air tanks with a certain positive or negative pressure. Power source 143 may include a directional valve 142, which can be a solenoid valve, rotary valve, pneumatic directional valve, electro-hydraulic directional valve, manual directional valve, piezoelectric valve, pinch valve, rotary valve, or rotary shear valve. In this embodiment, multiple power sources 143 are arranged one-to-one with multiple flow pools 140A of the carrier 140. The multiple power sources 143 are connected to the corresponding flow pools 140A of the carrier 140 through their reversing valves 142 and multiple pipelines 141.

[0140] The waste liquid storage module 145 has multiple interfaces for storing or discharging all or part of the waste liquid from the fluid system. Depending on functional requirements, the waste liquid storage module 145 may also include liquid detection sensors, air purification devices, or secondary spill prevention equipment. In this embodiment, the waste liquid storage module 145 includes multiple interfaces, and multiple power sources 143 are connected to the corresponding interfaces of the waste liquid storage module 145 via their reversing valves 142 and multiple pipelines 144.

[0141] The fluid storage device 153 is used to store liquids such as reagents, samples, and cleaning solutions required by the fluid system during biochemical detection (such as gene sequencing). Each type of liquid can correspond to a reagent storage area. In this embodiment, the fluid storage device 153 includes multiple reagent bottles, and the receiving portion of each reagent bottle constitutes a reagent storage area. Figure 8 The image shows 16 reagent bottles of the fluid storage device 153. The eight reagent bottles on the right are connected to the external flow channel interface 301 of the manifold body via eight reagent lines and a reversing valve 151. The eight reagent bottles on the left are connected to the external flow channel interfaces 341 to 348 of the manifold body via another eight reagent lines.

[0142] The pipette assembly is used to draw liquid from the fluid storage device 153 and deliver it externally. The pipette assembly may include a reagent tube, a reagent needle, or a manifold. The pipette assembly may also include a filter, a bubble sensor, etc.

[0143] Fluid piping assemblies are used to connect fluid devices and transport fluids. Fluid piping assemblies may include detection devices such as pressure sensors, flow sensors, and bubble sensors mounted on the pipes to monitor the state of the fluid system.

[0144] The reversing valve 151 is used to switch the connection relationships between different pipette components and different first external flow channel interfaces of the manifold body, so that samples or reagents are transported along a set path. For example, the internal flow channel control valve 309 has three ports: one port can be connected to the carrier 140, another port can be connected to the fluid storage device 153, and the third port can be connected to the reagent bottle. Thus, the reversing valve 151 can control whether the fluid storage device 153 is connected to the carrier 140. The reversing valve 151 can be a solenoid valve, rotary valve, pneumatic reversing valve, electro-hydraulic reversing valve, manual reversing valve, piezoelectric valve, pinch valve, rotary valve, swivel valve, etc. In this embodiment, the reversing valve 151 is a rotary valve. The rotary valve has eight ports, which are respectively connected to eight reagent bottles on the right side of the storage device 153.

[0145] The following description of the working process of the embodiment of this disclosure is based only on the working process of the flow channel related to the internal flow channel control valve 309. The working process of the flow channel related to the internal flow channel control valve 309 can be referred to for other internal flow channel control valves.

[0146] When the reversing valve 151 is rotated to switch to the 8th position and the internal flow control valve 309 is in the first position ( Figure 8 In the lower position, internal flow channel interfaces 318 and 328 are connected, and internal flow channel interface 338 is disconnected, so that the reagent from the reagent bottle connected to the reagent pipeline 152 enters the flow cell 140A of the carrier 140 connected to the external flow channel interface 358 via the reversing valve 151, reagent pipeline 150, external flow channel interface 301, internal flow channel 361, internal flow channel 363, internal flow channel 367, internal flow channel 378, internal flow channel interface 318, internal flow channel control valve 309, internal flow channel interface 328, internal flow channel 398 and external flow channel interface 358. When the internal flow channel control valve 309 is in the second position ( Figure 8 (Middle and upper position), internal flow channel interfaces 338 and 328 are connected, internal flow channel interface 318 is disconnected, and the reagent in the reagent bottle connected to the external flow channel interface 348 of the manifold body enters the flow cell 140A of the carrier 140 connected to the external flow channel interface 358 through the external flow channel interface 348, internal flow channel 388, internal flow channel interface 338, internal flow channel control valve 309, internal flow channel interface 328, internal flow channel 398 and external flow channel interface 358.

[0147] In this embodiment, the reagent bottles connected to the external flow channel interfaces 341 to 348 of the manifold body can be used independently, which can shorten the calling time and reduce the wear of the reversing valve.

[0148] The term "reagent" as used in all the above embodiments should be understood to include all fluids, including pure water and gases.

[0149] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this disclosure and not to limit them; although this disclosure has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications can still be made to the specific implementation of this disclosure or equivalent substitutions can be made to some technical features without departing from the spirit of the technical solutions of this disclosure, and all such modifications and substitutions should be covered within the scope of the technical solutions claimed in this disclosure.

Claims

1. A manifold assembly, comprising: The manifold body is a multi-manifold plate, including one or more first external flow channel interfaces, multiple first internal flow channel interfaces, multiple second internal flow channel interfaces, one or more second external flow channel interfaces, and multiple internal flow channels. The multiple internal flow channels include multiple first internal flow channels and multiple second internal flow channels. Each first external flow channel interface is connected to at least one first internal flow channel interface through a corresponding first internal flow channel, and each second external flow channel interface is connected to at least one second internal flow channel interface through a corresponding second internal flow channel. The multiple first internal flow channels or the multiple second internal flow channels form a tree-like branch structure. The multiple first internal flow channel interfaces are arranged side-by-side, and the multiple second internal flow channel interfaces are arranged side-by-side in the same direction as the multiple first internal flow channel interfaces. Furthermore, along a direction perpendicular to the arrangement direction of the multiple first internal flow channel interfaces, the multiple first internal flow channel interfaces and the multiple second internal flow channels are located between and spaced apart from the one or more first external flow channel interfaces and the one or more second external flow channels. Multiple internal flow control valves are arranged side-by-side on the same outer side of the multi-manifold plate, and the arrangement direction of the multiple internal flow control valves is the same as the arrangement direction of the multiple first internal flow interfaces and the arrangement direction of the multiple second internal flow interfaces. Each internal flow control valve is connected between at least one first internal flow interface and at least one second internal flow interface, and is configured to control the on / off state of the first internal flow interface and the second internal flow interface to which it is connected.

2. The manifold assembly according to claim 1, wherein, At least two of the first internal flow channels are connected to the corresponding first external flow channel interface through another first internal flow channel; and / or At least two of the second internal flow channels are connected to the corresponding second external flow channel interface through another second internal flow channel.

3. The manifold assembly according to claim 1, wherein, The internal flow channel control valve is a solenoid valve; and / or The internal flow channel control valve is a reversing valve.

4. The manifold assembly according to claim 1, wherein, At least one of the internal flow channel control valves is provided corresponding to one of the first internal flow channel interfaces and one of the second internal flow channel interfaces; and / or At least one of the internal flow channel control valves is provided corresponding to one first internal flow channel interface and two or more second internal flow channel interfaces; and / or At least one of the internal flow channel control valves is provided corresponding to two or more first internal flow channel interfaces and one second internal flow channel interface; and / or At least one of the internal flow channel control valves is provided corresponding to two or more first internal flow channel interfaces and two or more second internal flow channel interfaces.

5. The manifold assembly according to claim 1, wherein, The plurality of internal flow channels includes a third internal flow channel, which is connected to at least one of the second internal flow channels; The manifold body includes a third external flow channel interface, which is connected to the third internal flow channel. The manifold assembly includes a bypass control valve configured to control the opening and closing of the third internal flow passage connected thereto and the at least one second internal flow passage connected thereto.

6. The manifold assembly according to claim 5, wherein, The bypass control valve includes three ports, two of which are connected to two second internal flow channels respectively, and the other is connected to the third internal flow channel. The bypass control valve is configured to connect the two second internal flow channels and disconnect the third internal flow channel, or to connect one of the two second internal flow channels to the third internal flow channel and disconnect the other.

7. The manifold assembly according to any one of claims 1 to 6, wherein Multiple first external flow channel interfaces are arranged side-by-side on the same side of the multi-manifold plate; and / or Multiple second external flow channel interfaces are arranged side-by-side on the same side of the multi-manifold plate; and / or Multiple first internal flow channel interfaces and multiple second internal flow channel interfaces are arranged side by side on the same side of the multi-manifold plate.

8. A fluid system, comprising: The manifold assembly according to any one of claims 1 to 7; The fluid storage device (153) includes one or more reagent storage areas for storing reagents, the reagent storage areas being connected to a corresponding first external flow channel interface; A carrier plate (140) includes at least one flow cell (140A), each of said flow cells (140A) being connected to a corresponding second external flow channel interface of the manifold assembly; and One or more power sources (143) are configured to drive the reagents in the reagent storage area to the corresponding flow cell (140A).

9. The fluid system of claim 8 further includes a reversing valve (151) disposed between the fluid storage device (153) and one or more first external flow channel interfaces of the manifold body, and configured to selectively connect one of the reagent storage areas to one of the first external flow channel interfaces.

10. The fluid system according to claim 8 or 9 further includes a bypass line (146) configured to connect to at least one of the plurality of internal flow channels to allow reagents entering the manifold body (101) to flow into the bypass line (146).

11. The fluid system according to claim 8 or 9, wherein, The carrier (140) includes two or more flow pools (140A), and the fluid system includes two or more power sources (143) corresponding to the two or more flow pools (140A).

12. The fluid system according to claim 8 or 9 further includes a waste liquid storage module (145) configured to receive waste liquid in the flow tank (140A).

13. A biochemical analysis and detection platform, wherein, Includes a manifold assembly according to any one of claims 1 to 7 or a fluid system according to any one of claims 8 to 12.

14. The biochemical analysis and detection platform according to claim 13, wherein, Includes a gene sequencer, the gene sequencer including the manifold assembly or the fluid system.

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