Tobacco NtMYB61 gene, protein and application thereof

By cloning and editing the tobacco NtMYB61 gene and utilizing the CRISPR/Cas9 system, the problem of insufficient drought resistance in tobacco was solved. This achieved gene overexpression reduction and knockout to improve tobacco drought resistance, providing a breeding basis and molecular target, and enhancing the growth capacity of tobacco under drought stress.

CN119662662BActive Publication Date: 2025-10-28SICHUAN BRANCH OF CHINA TOBACCO
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Patent Information

Application Number
CN202411860465.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Priority Date
2024-12-06
Filing Date
2024-12-17
Publication Date
2025-10-28
Estimated Expiration
2044-12-17

AI Technical Summary

Technical Problem

Existing technologies are insufficient to effectively improve the drought resistance of tobacco, resulting in reduced tobacco yield and quality. Furthermore, the high labor costs associated with manual irrigation make it difficult to implement in most tobacco-growing areas.

Method used

By cloning the tobacco NtMYB61 gene and using the CRISPR/Cas9 gene editing system, gene overexpression and knockout were performed respectively to construct transgenic tobacco to improve or reduce its drought resistance.

Benefits of technology

Gene overexpression reduces tobacco drought resistance, while gene knockout increases it, providing a theoretical basis and molecular targets for genetic research and breeding of tobacco drought resistance, and enhancing the growth capacity of tobacco under drought stress.

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Abstract

This invention discloses the tobacco NtMYB61 gene, its protein, and its applications. An NtMYB61 gene capable of influencing tobacco's resistance to drought stress is disclosed, with its CDS sequence shown in SEQ ID No. 2 and its genome sequence shown in SEQ ID No. 1. This invention utilizes traditional gene overexpression and transgenic techniques to construct the NtMYB61 gene into a plant expression vector and transfer it into tobacco for overexpression. The overexpressed plants exhibited significantly reduced drought resistance compared to the wild-type control. Conversely, by using gene editing technology to knock out the NtMYB61 gene in the tobacco genome, the resulting Ntmyb61 mutant material exhibited significantly enhanced drought stress tolerance compared to the wild-type control.
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