Composition for removing senescent cells as well as preparation method and application of composition

Abstract

The invention discloses a composition for removing senescent cells as well as a preparation method and application of the composition. The compounded anti-aging composition comprises a silybum marianum extract, tetrahydrocurcumin, a hollyhock flower extract, sodium mannose phosphate, polydeoxyribonucleotide and collagen peptide, through mutual compatibility of the components, the effects of targeting aging cells and removing aging cells are achieved, meanwhile, a Maltese cross liquid crystal structure formed by alpha-gel is combined, and the anti-aging effect is achieved. The alpha-gel can better wrap other effective components and help the effective components to permeate into the deep layer of the skin, and the alpha-gel also has moisturizing and anti-aging effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin care products, and in particular relates to a composition for removing senescent cells, a preparation method and an application thereof. Background Art

[0002] Skin aging is a complex process, in which "senescent cells" play an important role. Senescent cells, or "zombie" cells, are a type of cells that no longer divide. They go through a senescence period, during which they neither die nor divide and reproduce normally, but like zombies, they continue to produce destructive molecules, attacking and killing nearby healthy cells. These senescent cells secrete some aging factors (SASP), triggering the aging of neighboring cells, including inflammatory factors involved in inflammatory responses and enzymes involved in skin matrix degradation, which can amplify micro-inflammation of the skin and destroy skin collagen, thereby affecting the surrounding normal cells and converting them into senescent cells. Surviving zombie cells will have DNA damage, telomere shortening, mitochondrial metabolic damage, cell cycle arrest, expression and aging-related biomarkers such as p53, p21, p16 activation, la minute B1 downregulation, increased activity of β-galactosidase (SA-β-gal) causing an increase in lysosome mass, etc., allowing the cells as a whole to enter a senescent state. Ultimately, zombie cells will affect the ECM extracellular matrix and cause overall skin aging.

[0003] Cells, as the most basic unit of the structure of living organisms, provide vitality and renewal for the skin. When cells are stimulated by exogenous stress and affected by endogenous factors, the skin will produce oxidative stress. Oxidative stress is also the main cause of cell damage. Oxidative stress can induce high levels of ROS production, leading to DNA oxidative damage to telomeres, and ultimately leading to the blockage of the cell renewal cycle and the promotion of cell aging. Therefore, to achieve root anti-aging, we must return to the cellular level.

[0004] The human body itself has a good clearance mechanism that causes senescent cells to undergo apoptosis. However, when a certain regulatory mechanism fails, for example, when healthy cells are subjected to molecular or cellular stress, such as DNA damage, oxidative free radicals, chemical damage, radiation damage or physical damage, this aging mechanism will be activated to transform these cells into senescent cells to prevent them from continuing to proliferate. Some damaged cells face apoptosis according to normal procedures, while some damaged cells are still alive and have metabolic activity, but have lost the ability to divide. These cells are so-called senescent cells, also known as "zombie" cells. Unlike apoptosis, zombie cells can resist death and survive for a long time, and continue to secrete various cytokines and proteases, infecting surrounding cells or destroying the normal structure of the body. "Zombie cells" produce SASP: pro-inflammatory factors poison nearby cells and release MMPs that destroy ECM, thereby accelerating skin aging. Therefore, to resist skin aging, the underlying logic is to remove zombie cells and restore cell vitality and energy.

[0005] There are many anti-aging products on the market, most of which focus on the following aspects: (1) Improving fine lines and wrinkles: Many anti-aging products aim to improve fine lines and wrinkles on the skin by adding ingredients such as collagen and hyaluronic acid, making the skin smoother and firmer. (2) Improving skin elasticity: Products containing ingredients such as elastin and peptides can enhance skin elasticity and reduce sagging. (3) Moisturizing and firming: Moisturizing is an important part of anti-aging. Many products add moisturizing factors such as glycerin and sodium hyaluronate to maintain skin moisture. At the same time, firming ingredients such as caffeine and hexapeptide can also help improve skin firmness. (4) Antioxidant: Antioxidant ingredients such as vitamin C, vitamin E, and green tea extract can resist free radical damage to the skin, thereby slowing down the aging process. It is impossible to target a specific cell or tissue for anti-aging.

[0006] Most anti-aging products on the market can only act on the surface of the skin, and it is difficult to penetrate into the dermis or subcutaneous tissue for deep anti-aging. This limits the anti-aging effect of the product. In addition, the high content of active ingredients may cause skin discomfort or allergic reactions after use. Long-term use of certain anti-aging products may also bring some side effects, such as thinning of the skin, dependence, etc., resulting in limited product effects. In addition, conventional anti-aging products on the market achieve anti-aging by directly supplementing collagen, and do not clear senescent cells. Moreover, the products lack targeted technology and have unstable structures, making it difficult to accurately act on senescent cells or tissues. Therefore, developing a product that can effectively remove senescent cells and achieve deep anti-aging is an urgent problem that needs to be solved at present. Summary of the invention

[0007] In order to effectively remove senescent cells and achieve deep anti-aging, the present invention provides a composition for removing senescent cells, a preparation method and an application thereof.

[0008] The present invention provides a composition for removing senescent cells, comprising the following components in percentage by mass: 10-13% of a moisturizer, 10-15% of an emollient, 0.1-0.3% of a thickener, 1.5-3.5% of an α-gel emulsifier, 5.1-23% of an anti-aging composition, 0.02-0.1% of a pH regulator, and the remainder being water, wherein the sum of the percentages by mass of the above components is 100%;

[0009] Among them, the anti-aging composition is milk thistle extract, tetrahydrocurcumin, hibiscus flower extract, sodium mannose phosphate, polydeoxyribonucleotide, and collagen peptide.

[0010] Furthermore, the anti-aging composition includes the following components in mass percentage: milk thistle extract: 0.5-3%, tetrahydrocurcumin: 2-6%, hibiscus flower extract: 0.5-2.5%, sodium mannose phosphate: 0.5-2%, polydeoxyribonucleotide: 0.5-4%, collagen peptide 1-3%, palmitoyl tetrapeptide-7, 0.1-1.5%. The sum of the mass percentages of the above components is 5.1-23%.

[0011] Wherein, the moisturizing agent is one or a combination of glycerin, butylene glycol, isopentyl glycol, hexylene glycol, pentylene glycol, panthenol, propylene glycol, methyl gluceth 20, glycereth-26, sorbitol, and polyethylene glycol 400.

[0012] Among them, emollients are composed of liquid oils and solid oils;

[0013] Furthermore, the liquid oil is at least one of dimethicone, cetyl dimethicone, squalane, caprylic / capric triglyceride, diethylhexyl carbonate, cetyl ethylhexanoate, meadowfoam seed oil, rosehip oil, jojoba oil, wheat germ oil, sweet almond oil, tangerine peel oil, and vitamin E;

[0014] Furthermore, the solid oil is at least one of synthetic wax, beeswax, shea butter, sunflower seed wax, mixed base polydimethylsiloxane cross-linked polymer, candelilla wax, lanolin, white cocoa butter, and green vitex flower wax.

[0015] The α-gel emulsifier is one or a combination of olive oil polyglycerol-6 esters, sodium stearoyl lactylate, cetearyl alcohol, and cetyl alcohol phosphate.

[0016] Wherein, the thickener is: one or a combination of carbomer, xanthan gum, and hydroxyethyl cellulose;

[0017] Wherein, the pH regulator is any one of sodium hydroxide, triethanolamine or arginine.

[0018] Based on the same inventive concept, the present invention also provides a method for preparing the above composition, comprising the following steps:

[0019] Dissolve the aqueous phase raw material and the oil phase raw material separately, then add the oil phase into the aqueous phase, stir homogenously, add a pH regulator, adjust the pH to 6.0-6.5, stir evenly, add the anti-aging composition for removing senescent cells and stir for 20-35 minutes to obtain;

[0020] Further, the water phase raw materials include: a humectant, a thickener, and water;

[0021] Furthermore, the oil phase raw materials include: emollients and α-gel emulsifiers.

[0022] Based on the same inventive concept, the present invention also provides the use of the above composition in preparing skin care products.

[0023] Furthermore, skin care products include lotions, essences, creams, facial ointments, and facial masks.

[0024] The beneficial effects of the present invention are:

[0025] The composite anti-aging composition of the present invention comprises milk thistle extract, tetrahydrocurcumin, hibiscus flower extract, sodium mannose phosphate, polydeoxyribonucleotides, and collagen peptides. By combining the above ingredients with each other and the Maltese cross liquid crystal structure formed by α-gel, the senescent cells can be lysed and the anti-aging effect can be enhanced; at the same time, inflammatory aging can be inhibited, skin aging can be prevented, the true epidermis of aging skin can be repaired and tightened, and the firmness of the skin can be improved. The α-gel can stably wrap the composition in its Maltese cross liquid crystal structure. The safety problems of existing products are overcome, and the defects of the existing anti-aging products, such as single structural components and limited effects, are improved.

[0026] The composition for removing senescent cells prepared by the present invention includes three pathways for preventing the generation of senescent cells: milk thistle extract, removing senescent cells in time: hibiscus flower extract, tetrahydrocurcumin, sodium mannose phosphate, and inhibiting the senescence-associated secretory phenotype (SASP): polydeoxyribonucleotide, which comprehensively resist aging, enhance transdermal penetration, promote absorption, and can target senescent cells; the spherical structure of the anti-aging composition is stably present in the α-gel structure, so that the anti-aging composition and the α-gel play a dual role, which can not only inhibit the crystallization of oil and fat and make the preparation more stable, but also the α-gel itself has moisturizing and anti-aging effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The present invention is further described below in conjunction with the accompanying drawings.

[0028] Figure 1 Volunteers took photos of the anti-aging effect using the VISA instrument. From left to right, they are unused (0W), used for 2 weeks (2W), and used for 4 weeks (4W).

[0029] Figure 2 The anti-wrinkle effects of the composition product prepared in Example 4 on volunteers after 0 days, 28 days and 56 days.

[0030] Figure 3 This is a picture taken using the negative staining technique of the composition prepared in Example 4 of the present invention.

[0031] Figure 4 This is a transmission electron microscope image of the composition prepared in Comparative Example 2 of the present invention.

[0032] Figure 5 This is a three-dimensional fluorescence image of the composition prepared in Example 4 of the present invention targeting senescent cells.

[0033] Figure 6 The three-dimensional fluorescence images of the compositions prepared in Example 4 of the present invention and Comparative Example 1 targeting mitochondria.

[0034] Figure 7 This is a real picture of the composition prepared in Example 4 of the present invention under a polarizing microscope.

[0035] Figure 8 This is a real picture of the composition prepared in Comparative Example 2 of the present invention under a polarizing microscope. DETAILED DESCRIPTION

[0036] In order to better understand the present invention, the present invention is explained in detail below through the description of preferred specific embodiments of the present invention, but the present invention is not limited in any form.

[0037] Unless otherwise specified, the raw materials or chemical reagents used in the embodiments of the present invention are obtained through conventional commercial channels.

[0038] Some raw material sources:

[0039] Milk thistle extract: Raw material trade name: Celyscence, specification: 1kg, manufacturer: Biostar Korea;

[0040] Tetrahydrocurcumin: Raw material trade name: Polyglycoside® spherical curcumin. Specification: 1Kg, manufacturer: Shaanxi Baihong Synthetic Biotechnology Co., Ltd.;

[0041] Althaea flower extract: Raw material trade name: ALTHEOSTEM™ 73540, specification: 1KG, manufacturer: Provital;

[0042] Mannose Sodium Phosphate: Raw material trade name: Mannose-6-phosphate (M6P), specification: 10g, manufacturer: Beijing Youran Biotechnology Co., Ltd.

[0043] Polydeoxyribonucleotide: Raw material trade name: Polyglycoside® microsphered PDRN. Specification: 1Kg, manufacturer: Shaanxi Baihong Synthetic Biotechnology Co., Ltd.;

[0044] Collagen peptide: Taishu® collagen peptide PF, specification: 1Kg, manufacturer: Shaanxi Future Peptide Biotechnology Co., Ltd.;

[0045] α-Gel emulsifier (cetyl alcohol phosphate), raw material trade name: NIKKOL purephosα Specification: 1KG, manufacturer: NIKKOL.

[0046] Example 1

[0047] This embodiment provides a method for preparing a composition for removing senescent cells:

[0048] First, the following raw materials are weighed, wherein the content of each component is 13% of moisturizer, 15% of emollient, 0.2% of thickener, 1.5% of α-gel, 10.5% of senescent cell removing composition, 0.02-0.1% of pH adjuster, and the remainder is water, and the sum of the mass percentages of the above components is 100%.

[0049] Step 1: Add the aqueous phase raw material into the reaction vessel, heat to 85°C, stir and dissolve until transparent.

[0050] Keep warm for 25 minutes;

[0051] The water phase raw materials include 13% of a moisturizer consisting of butylene glycol, dipropylene glycol, sodium hyaluronate, and glycerin, 0.2% of carbomer, and an appropriate amount of purified water;

[0052] Step 2: Dissolve the oil phase, which consists of emollients and α-gel emulsifiers.

[0053] Add 15% of the emollient composed of caprylic / capric triglyceride, diethylhexyl carbonate, cetyl ethylhexanoate, avocado esters, and jojoba oil, and 1.5% of the α-gel emulsifier: cetyl phosphate into the oil phase mixing tank, start stirring, adjust the speed until the liquid surface shakes up and down, and heat the mixing tank to 85-87°C at the same time, stirring until all the oil is dissolved.

[0054] Step 3: Emulsification, pump the oil phase obtained in step 2 into the main water phase pot, maintain the temperature at 83-87°C, stir while pumping, and adjust the speed until the liquid surface shakes up and down. After pumping, maintain the temperature at 82-85°C, turn on the homogenization stirring, and homogenize at 50Hz for 10 minutes.

[0055] Step 4: First prepare 10.5% of the anti-aging composition for removing senescent cells, including 2% tetrahydrocurcumin, 1% polydeoxyribonucleotide, 1% palmitoyl tetrapeptide-7, 1%, 2% hibiscus flower extract, 2% milk thistle extract, 1.5% sodium mannose phosphate, and 1% collagen peptide.

[0056] After the homogenization in step 3 is completed, the circulating water is turned on to cool the temperature to 54-60°C, a pH adjuster is added to adjust the pH of the material to 6.5, and the mixture is stirred evenly. When the temperature drops to 40-45°C, the anti-aging composition for removing senescent cells is added, the rotation speed is adjusted until the liquid surface shakes up and down, and the mixture is stirred for 35 minutes.

[0057] Example 2

[0058] This embodiment provides a method for preparing a composition for removing senescent cells:

[0059] First, the following raw materials are weighed, wherein the content of each component is 10% of moisturizer, 10% of emollient, 0.1% of thickener, 2.5% of α-gel, 7.3% of senescent cell removing composition, 0.02-0.1% of pH adjuster, and the balance is water, and the sum of the mass percentages of the above components is 100%.

[0060] Step 1: Add the aqueous phase raw material into the reaction vessel, heat to 85°C, stir and dissolve until transparent.

[0061] Keep warm for 25 minutes;

[0062] The water phase raw materials include 10% of a moisturizer consisting of butylene glycol, dipropylene glycol, sodium hyaluronate, and glycerin, 0.2% of carbomer, and an appropriate amount of purified water;

[0063] Step 2: Dissolve the oil phase, which consists of emollients and α-gel emulsifiers.

[0064] Add 10% of the emollient composed of caprylic / capric triglyceride, diethylhexyl carbonate, cetyl ethylhexanoate, avocado esters, and jojoba oil, and 2.5% of the α-gel emulsifier: cetyl phosphate into the oil phase mixing tank, start stirring, adjust the speed until the liquid surface shakes up and down, and heat the mixing tank to 85-87°C at the same time, stirring until all the oil is dissolved.

[0065] Step 3: Emulsification, pump the oil phase obtained in step 2 into the main water phase pot, maintain the temperature at 83-87°C, stir while pumping, and adjust the speed until the liquid surface shakes up and down. After pumping, maintain the temperature at 82-85°C, turn on the homogenization stirring, and homogenize at 50Hz for 10 minutes.

[0066] Step 4: First prepare a 7.3% anti-aging composition for removing senescent cells, including 3% tetrahydrocurcumin, 0.5% polydeoxyribonucleotide, 0.3% palmitoyl tetrapeptide-7, 1% hibiscus flower extract, 0.5% sodium mannose phosphate, and 2% collagen peptide;

[0067] After cooling and adding other ingredients and homogenizing in step 3, start circulating water to cool the temperature to 54-60°C, add pH adjuster, adjust the pH of the material to 6.5, stir evenly, wait until the temperature drops to 40-45°C, add the anti-aging composition for removing senescent cells, adjust the speed until the liquid surface shakes up and down, and stir for 35 minutes.

[0068] Example 3

[0069] This embodiment provides a method for preparing a composition for removing senescent cells:

[0070] First, the following raw materials are weighed, wherein the content of each component is 12% of moisturizer, 13% of emollient, 0.2% of thickener, 1.5% of α-gel, 8.5% of senescent cell removing composition, 0.02-0.1% of pH adjuster, and the balance is water, and the sum of the mass percentages of the above components is 100%.

[0071] Step 1: Add the aqueous phase raw material into the reaction vessel, heat to 85°C, stir and dissolve until transparent.

[0072] Keep warm for 25 minutes;

[0073] The water phase raw materials include 12% of a moisturizer consisting of butylene glycol, dipropylene glycol, sodium hyaluronate, and glycerin, 0.2% of carbomer, and an appropriate amount of purified water;

[0074] Step 2: Dissolve the oil phase, which consists of emollients and α-gel emulsifiers.

[0075] Add 13% of the emollient composed of caprylic / capric triglyceride, diethylhexyl carbonate, cetyl ethylhexanoate, avocado esters, and jojoba oil, and 1.5% of the α-gel emulsifier: cetyl phosphate into the oil phase mixing tank, start stirring, adjust the speed until the liquid surface shakes up and down, and heat the mixing tank to 85-87°C at the same time, stirring until all the oil is dissolved.

[0076] Step 3: Emulsification, pump the oil phase obtained in step 2 into the main water phase pot, maintain the temperature at 83-87°C, stir while pumping, and adjust the speed until the liquid surface shakes up and down. After pumping, maintain the temperature at 82-85°C, turn on the homogenization stirring, and homogenize at 50Hz for 10 minutes.

[0077] Step 4: First prepare 8.5% of the anti-aging composition for removing senescent cells, including 1.5% polydeoxyribonucleotides, 0.5% palmitoyl tetrapeptide-7, 2% hibiscus flower extract, 2% sodium mannose phosphate, 1% collagen peptide, and 1.5% tetrahydrocurcumin;

[0078] After cooling and adding other ingredients and homogenizing in step 3, start circulating water to cool the temperature to 54-60°C, add pH adjuster, adjust the pH of the material to 6.5, stir evenly, wait until the temperature drops to 40-45°C, add the anti-aging composition for removing senescent cells, adjust the speed until the liquid surface shakes up and down, and stir for 35 minutes.

[0079] Example 4

[0080] This embodiment provides a method for preparing a composition for removing senescent cells:

[0081] First, the following raw materials are weighed, wherein the content of each component is 13% of moisturizer, 15% of emollient, 0.2% of thickener, 1.5% of α-gel, 13.5% of senescent cell removing composition, 0.02-0.1% of pH adjuster, and the balance is water, and the sum of the mass percentages of the above components is 100%.

[0082] Step 1: Add the aqueous phase raw material into the reaction vessel, heat to 85°C, stir and dissolve until transparent.

[0083] Keep warm for 25 minutes;

[0084] The water phase raw materials include 13% of a moisturizer consisting of butylene glycol, dipropylene glycol, sodium hyaluronate, and glycerin, 0.2% of carbomer, and an appropriate amount of purified water;

[0085] Step 2: Dissolve the oil phase, which consists of emollients and α-gel emulsifiers.

[0086] Add 15% of the emollient composed of caprylic / capric triglyceride, diethylhexyl carbonate, cetyl ethylhexanoate, avocado esters, and jojoba oil, and 1.5% of the α-gel emulsifier: cetyl phosphate into the oil phase mixing tank, start stirring, adjust the speed until the liquid surface shakes up and down, and heat the mixing tank to 85-87°C at the same time, stirring until all the oil is dissolved.

[0087] Step 3: Emulsification, pump the oil phase obtained in step 2 into the main water phase pot, maintain the temperature at 83-87°C, stir while pumping, and adjust the speed until the liquid surface shakes up and down. After pumping, maintain the temperature at 82-85°C, turn on the homogenization stirring, and homogenize at 50Hz for 10 minutes.

[0088] Step 4: First prepare a 13.5% anti-aging composition for removing senescent cells, including 3% milk thistle extract, 2% polydeoxyribonucleotide, 1.5% palmitoyl tetrapeptide-7, 2.5% hibiscus flower extract, 1% sodium mannose phosphate, 2% collagen peptide, and 1.5% tetrahydrocurcumin;

[0089] After the homogenization in step 3 is completed, the circulating water is turned on to cool the temperature to 54-60°C, a pH adjuster is added to adjust the pH of the material to 6.5, and the mixture is stirred evenly. When the temperature drops to 40-45°C, the anti-aging composition for removing senescent cells is added, the rotation speed is adjusted until the liquid surface shakes up and down, and the mixture is stirred for 35 minutes.

[0090] Comparative Example 1

[0091] The difference between this comparative example and Example 1 is that in step 4, no senescent cell removing anti-aging composition component is added, and the rest is the same as Example 1.

[0092] Comparative Example 2

[0093] The difference between this comparative example and Example 1 is that no α-gel emulsifier component is added in step 2, and the rest is the same as Example 1.

[0094] Test Example 1

[0095] Anti-aging efficacy evaluation:

[0096] Test method: Volunteer use test.

[0097] Sample size: The total number of volunteers for sample distribution was 26, and 24 questionnaires were collected for a valid sample size.

[0098] Among the volunteers who participated in the test, 14 had mixed skin, 5 had oily skin, 3 had normal skin, and 2 had sensitive skin. All volunteers thought that the essence was mild and non-irritating, and no one experienced adverse reactions during use.

[0099] Among the 24 volunteers, based on their subjective evaluation, 100% of the volunteers believed that the essence had a certain moisturizing effect after use, and improved dry and rough skin conditions, making the skin hydrated; 95.83% of the volunteers believed that the skin condition was improved and the skin became firmer, and the skin elasticity was increased after use of the essence; 100% of the volunteers believed that the fine lines on the facial skin were reduced and the superficial wrinkles were lightened after use of the essence; 79.17% of the volunteers believed that the superficial wrinkles around the eyes were lightened and the deeper wrinkles around the eyes were lighter after use of the essence; 75.00% of the volunteers believed that the nasolabial folds and forehead wrinkles were reduced, the deeper wrinkles on the forehead were lighter, and the superficial wrinkles were lighter after use of the essence.

[0100] Human efficacy evaluation test, volunteers were selected to take photos and evaluate the improvement of wrinkles using the VISA instrument. Figure 1 The photographs taken by the instrument show that the volunteers' wrinkles were significantly improved after using the product of the present invention for 4 weeks.

[0101] Test Example 2

[0102] Quantitative assessment of skin wrinkle changes:

[0103] PRIMOS CR-3D is a non-contact in vivo skin measurement device based on structured light projection. This study evaluated the depth of wrinkles and the volume of wrinkles. The smaller the parameter value, the better the fine line condition of the skin. The sensor can evaluate the surface characteristics of the skin. The volunteers were between 30 and 65 years old. We chose periocular wrinkles as reference data. Figure 2 It can be found that the use of the product of Example 4 for 28 days and 56 days has a significant effect on improving the depth of wrinkles, while in Comparative Example 1, in the product without the anti-aging composition, the depth and volume of wrinkles are basically unchanged. It can be concluded that the anti-aging composition of the present invention has the effect of improving the depth of wrinkles and can play an anti-aging effect.

[0104] Test Example 3

[0105] The compositions prepared in Example 4 and Comparative Example 1 were photographed using a transmission electron microscope and a glow radiator using negative staining technology. The results are as follows: Figure 3 In Example 4, an anti-aging composition was added. Under negative staining technology, a large number of regular and uniform spherical particles with a particle size of 100-200 nm can be observed, which are stably present in the emulsion. The anti-aging active ingredients are wrapped inside the microspheres to exert a stable and long-lasting effect. In Comparative Example 1, no anti-aging composition was added. Figure 4 Under the transmission electron microscope, there is basically no microsphere structure, it is just a stick-shaped structure of ordinary cream, without the microsphere structure, and cannot exert precise anti-aging effects.

[0106] Test Example 4

[0107] Targeting senescent cells assays:

[0108] The fluorescent labeling method was used to label cells with fluorescent dyes or fluorescent proteins, and the cell structure and dynamic changes were observed, which proved that the polyamino acid polysaccharide condensate in the anti-aging composition can target senescent cells. In the experiment, red marked the product containing the anti-aging composition, blue marked the cell nucleus, and green was the cytoskeleton. After the polyamino acid polysaccharide condensate in the anti-aging composition with fluorescent labeling was co-cultured with cells, the cell nucleus and cytoskeleton were labeled with fluorescent dyes, and the co-localization was observed under a fluorescence microscope. The fluorescence results showed that red fluorescently labeled substances were observed inside the cells, indicating that the polyamino acid polysaccharide condensate in the anti-aging composition can target senescent cells. Figure 5It can be seen that the anti-aging composition prepared in Example 4 is marked with red fluorescence and can target senescent cells, but has no targeting effect on normal cells.

[0109] Test Example 5

[0110] Targeted mitochondrial assays:

[0111] The fluorescent labeling method was used to label cells with fluorescent dyes or fluorescent proteins, and the cell structure and dynamic changes were observed. This proved that tetrahydrocurcumin in the anti-aging composition can target the mitochondria in aging cells. In the experiment, red marked the product containing the anti-aging composition, blue marked the cell nucleus, and green was the cytoskeleton. After the polyamino acid polysaccharide condensate in the fluorescently labeled anti-aging composition was co-cultured with the cells, the cell nucleus and cytoskeleton were labeled with fluorescent dyes, and the co-localization was observed under a fluorescence microscope. The fluorescence results showed that red fluorescently labeled substances were observed inside the cells, indicating that tetrahydrocurcumin in the anti-aging composition can target the mitochondria in aging cells. Figure 6 It can be seen that the anti-aging composition prepared in Example 4 can target the mitochondria in aging cells, wherein the red fluorescent marker indicates that a large amount of red markers enter the mitochondria, while in Comparative Example 1, only a small amount of red markers enter.

[0112] Test Example 6

[0113] Composition structure: Figure 7 The composition prepared in Example 1 was photographed under a polarizing microscope, and a bright Maltese cross liquid crystal structure can be seen. The microsphere structure in the anti-aging composition is firmly wrapped in its liquid crystal structure, so that the active ingredients can play a better role. In Comparative Example 2, no liquid crystal structure emulsifier was added. When photographed under a polarizing microscope, as shown in FIG. Figure 8 , basically there is no liquid crystal structure, it is a relatively stable popular emulsification result. It is not long-lasting and stable, so it is not good at anti-aging effect.

[0114] Although preferred embodiments of the present invention have been described, additional changes and modifications may occur to these embodiments once those skilled in the art are aware of the basic inventive concepts.

[0115] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A composition for removing senescent cells, characterized in that: The composition comprises the following components in percentage by mass: 10-13% moisturizing agent, 10-15% emollient, 0.1-0.3% thickener, 1.5-3.5% α-gel emulsifier, 5.1-23% anti-aging composition, 0.02-0.1% pH regulator, and the balance is water. The sum of the percentages by mass of the above components is 100%; The anti-aging composition comprises milk thistle extract, tetrahydrocurcumin, hibiscus flower extract, sodium mannose phosphate, polydeoxyribonucleotide and collagen peptide.

2. A composition for removing senescent cells according to claim 1, characterized in that: The anti-aging composition comprises the following components in percentage by mass: 0.5-3% of milk thistle extract, 2-6% of tetrahydrocurcumin, 0.5-2.5% of hibiscus flower extract, 0.5-2% of sodium mannose phosphate, 0.5-4% of polydeoxyribonucleotide, 1-3% of collagen peptide, and 0.1-1.5% of palmitoyl tetrapeptide-7. The sum of the percentages by mass of the above components is 5.1-23%.

3. The composition for removing senescent cells according to claim 1, characterized in that: The moisturizing agent is one or a combination of glycerin, butylene glycol, isopentyl glycol, hexylene glycol, pentylene glycol, panthenol, propylene glycol, methyl gluceth 20, glycereth-26, sorbitol, and polyethylene glycol 400.

4. The composition for eliminating senescent cells according to claim 1, characterized in that: The emollient is composed of liquid grease and solid grease; The liquid oil is at least one of dimethicone, cetyl dimethicone, squalane, caprylic / capric triglyceride, diethylhexyl carbonate, cetyl ethylhexanoate, meadowfoam seed oil, rosehip oil, jojoba oil, wheat germ oil, sweet almond oil, tangerine peel oil, and vitamin E; The solid oil is at least one of synthetic wax, beeswax, shea butter, sunflower seed wax, mixed-base polydimethylsiloxane cross-linked polymer, candelilla wax, lanolin, white cocoa butter, and green wattle flower wax.

5. The composition for eliminating senescent cells according to claim 1, characterized in that: The α-gel emulsifier is one or a combination of olive oil polyglycerol-6 esters, sodium stearoyl lactylate, cetearyl alcohol, and cetyl alcohol phosphate.

6. The composition for eliminating senescent cells according to claim 1, characterized in that: The thickener is: one or a combination of carbomer, xanthan gum, and hydroxyethyl cellulose; The pH regulator is any one of sodium hydroxide, triethanolamine or arginine.

7. A method for preparing the composition for eliminating senescent cells according to any one of claims 1 to 6, characterized in that: The following steps are involved: Dissolve the aqueous phase raw material and the oil phase raw material separately, then add the oil phase into the aqueous phase, stir homogenously, add a pH regulator, adjust the pH to 6.0-6.5, stir evenly, add the anti-aging composition for removing senescent cells and stir for 20-35 minutes to obtain; The water phase raw materials include: moisturizer, thickener, water; The oil phase raw materials include: emollient and α-gel emulsifier.

8. Use of the composition for removing senescent cells according to any one of claims 1 to 6 in the preparation of skin care products.

9. The use according to claim 8, characterized in that: The skin care products include lotions, essences, facial creams, facial ointments and facial masks.

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