用于扩增I型登革病毒基因组的引物组、试剂盒及其方法和应用
By designing primer sets and third-generation sequencing technology for targeted amplification of the dengue virus type I genome, the problems of low genome coverage and cumbersome procedures in existing technologies have been solved, achieving high coverage and low cost detection results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ZHUHAI INT TRAVEL HEALTH CARE CENT
- Filing Date
- 2024-11-28
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies for dengue virus type I genome detection suffer from low genome coverage, a large number of primers, and cumbersome procedures, especially in next-generation sequencing and existing primer sets, resulting in high detection costs and low accuracy.
A primer set for targeted amplification of the dengue virus type I genome was designed, containing 20 nucleic acid sequences divided into two primer pools. Combined with third-generation sequencing technology, through reverse transcription, amplification, library construction and sequencing steps, high coverage and high throughput detection of the full-length genome were achieved.
This method achieves high coverage detection of the dengue virus type I genome, reduces detection costs, simplifies the sequence assembly process, and improves detection accuracy and efficiency.
Smart Images
Figure CN119710086B_ABST