Sample preparation facility for pesticide residue detection

Abstract

This invention relates to the field of pesticide residue detection technology, and more particularly to a sample preparation mechanism for pesticide residue detection, comprising a main control device, a sample storage and retrieval device, and a conveying device. The main control device coordinates and controls the various devices to achieve automated preparation of the sample for pesticide residue detection. Compared with traditional pesticide residue detection methods, this sample preparation mechanism for pesticide residue detection automatically realizes the functional steps of sample preparation, sample transfer, reagent or enzyme addition, mixing of the sample, and substrate addition through the cooperation of the sample storage and retrieval device and the conveying device. It has the following advantages: 1. The structural design is reasonable, enabling the various device components to cooperate with each other and reducing the floor space; 2. In the process of pesticide residue detection, it no longer relies on the professional operation of personnel to prepare the sample, which helps to improve the efficiency of pesticide residue detection, reduce labor costs, and reduce the cost of pesticide residue detection.

Description

Technical Field

[0001] This invention relates to the field of pesticide residue detection technology, and in particular to a sample preparation mechanism for pesticide residue detection. Background Technology

[0002] Pesticides are widely used for controlling crop diseases and pests due to their high efficiency, speed, economy, and convenience. However, they can also lead to pesticide residues in agricultural products, posing a threat to food safety. Therefore, pesticide residue detection equipment is needed to test agricultural products for pesticide residues. Currently, most pesticide residue testing is done using conventional laboratory testing equipment. However, this type of equipment cannot automatically add buffer solutions, enzymes, reagents, substrates, etc., requiring manual testing by professional technicians. It suffers from low automation, low testing efficiency, and is also bulky and inconvenient.

[0003] With the development of the industry, some portable pesticide residue detection devices have gradually emerged. For example, the utility model patent with publication number CN216900223, entitled "An Integrated Intelligent Detection Device for Qualitative and Quantitative Pesticide Residues," discloses a pesticide residue detection device. According to its specification and drawings, this integrated intelligent detection device includes a first turntable, a second turntable, and a liquid addition module. The upper surface of the first turntable has a sample tank and a reagent tank, while the upper surface of the second turntable has a reaction tank. The liquid addition module is located outside the two turntables, between them. It draws liquid samples from the sample tank and reagent tank and transfers them to the reaction tank for pesticide residue detection after reaction. However, this device has two parallel circular turntables, and the liquid addition module is installed between them, requiring multiple drive motor components. This results in a complex structure, a large footprint, and high implementation costs. Summary of the Invention

[0004] To overcome the problems existing in related technologies, this invention provides a sample preparation mechanism for pesticide residue detection. Based on the automated preparation of pesticide residue detection liquid samples, the structure is more reasonable, occupies less space and has a smaller volume, and the detection cost is lower.

[0005] To achieve the above objectives, the present invention provides a sample preparation mechanism for pesticide residue detection, comprising: a main control device, a sample storage and retrieval device and a conveying device electrically connected to the main control device.

[0006] The liquid sample storage and retrieval device includes a first storage module, a second storage module, and a liquid sample transfer module.

[0007] The first storage module includes a sample tray, a reaction tray, and a first driving component. The sample tray has multiple sample positions and a sample dispensing window, with each sample position used to place a sample vial. The reaction tray is installed below the sample tray and has multiple cuvette positions for placing cuvettes. Each cuvette position corresponds to a sample dispensing window, through which liquid samples are injected into the cuvettes. The first driving component is located below the sample tray and is fixedly connected to it. The first driving component drives the sample tray to rotate, thereby rotating the sample vials on it. The rotation of the sample tray drives the rotation of the reaction tray below it, thereby rotating the cuvettes on the reaction tray.

[0008] The second storage module includes a reagent tray with multiple reagent positions for storing enzymes or reagents, and a shaft hole in the center of the reagent tray.

[0009] The liquid sample transfer module includes a robotic arm, a liquid dispensing needle, and a second drive assembly. The liquid dispensing needle is installed at the first end of the robotic arm and is used to extract and release liquid samples. The first end of the robotic arm passes through the shaft hole of the reagent tray, so that the liquid dispensing needle is located above the reagent position. The second drive assembly is installed below the reagent tray and connected to the second end of the robotic arm. The second drive assembly is used to drive the robotic arm to lift or rotate, thereby driving the liquid dispensing needle to lift or rotate.

[0010] The delivery device is connected to the liquid injection needle through a pipe and is used to drive the liquid injection needle to draw or release liquid samples; the first driving component drives the sample tray and the reaction tray to rotate, and the second driving component drives the robotic arm to move the liquid injection needle up and down in a timely manner, and drives the robotic arm to rotate the liquid injection needle in a timely manner, so that the liquid injection needle reaches each of the sample positions, each of the cuvette positions, and each of the reagent positions to draw or release liquid samples.

[0011] In an optional embodiment, the sample tray is a circular disk with multiple sample positions arranged in a ring around its edge, and multiple sample application windows arranged in a ring around the inner side of each sample position; the reagent tray is crescent-shaped and arranged side-by-side with the sample tray, with multiple reagent positions arranged in an arc on the side of the reagent tray away from the sample tray, and an arc-shaped portion concave towards its center on the side of the reagent tray closer to the sample tray; the outer edge of the sample tray mates with the arc-shaped portion of the reagent tray, and a gap exists between them; the robotic arm drives the liquid application needle onto the reagent tray. When the robot arm rotates, it moves the dispensing needle to each of the reagent positions to draw reagents. When the robot arm drives the dispensing needle to rotate to a first preset position outside the reagent tray, the sample tray is driven to rotate, so that the sample bottles on the sample positions move sequentially to below the dispensing needle to realize liquid sample injection or extraction. When the robot arm drives the dispensing needle to rotate to a second preset position outside the reagent tray, the sample tray is driven to rotate, which drives the reaction tray to rotate, so that the cuvettes on the cuvette positions move sequentially to below the dispensing needle to realize liquid sample injection or extraction.

[0012] In an optional embodiment, the outer circumference of the sample tray is provided with an abutment platform; the first storage module further includes a sample receiving groove and a cuvette receiving groove; the sample receiving groove is located below the sample tray, and the top of its outer ring sidewall abuts against the abutment platform of the sample tray; the sample receiving groove has a first annular space; the opening of the first annular space faces each of the sample positions, for accommodating and providing rotation space for the sample bottles; the cuvette receiving groove is located below the reaction tray and inside the sample receiving groove, and has a second annular space; the opening of the second annular space faces each of the cuvette positions, for accommodating and providing rotation space for the cuvettes; an annular heating plate is provided on the sidewall of the cuvette receiving groove to maintain the reaction temperature of the test liquid in the cuvette.

[0013] In an optional embodiment, the reaction disk is provided with a plurality of detection ports along its radial direction, each detection port corresponding to each cuvette position; a first detection window is provided on the outer wall of the cuvette receiving tank, and a second detection window is provided on its inner wall, the first detection window and the second detection window being located on both sides of the cuvette position; the transmitting end of the spectrophotometer, the cuvette, and the receiving end of the spectrophotometer are arranged sequentially along the radial direction of the reaction disk.

[0014] In an optional embodiment, the sample disk has a central shaft hole; the first drive assembly includes a first motor, a first synchronous pulley, a second synchronous pulley, a first synchronous belt, a first bearing bracket, a first bearing, a second bearing, and a first rotating shaft; the first motor is mounted below the sample disk; the first synchronous pulley is sleeved on the output shaft of the first motor; the second synchronous pulley is arranged side by side with the first synchronous pulley and is connected by the first synchronous belt; the first bearing is mounted on the first bearing bracket and is located above the second synchronous pulley; the second bearing is mounted on the first bearing bracket and is located below the second synchronous pulley; the first rotating shaft is mounted on the first bearing bracket via the first bearing and the second bearing, the upper end of the first rotating shaft passes sequentially through the shaft center of the first bearing and the shaft hole of the sample disk and is fixedly connected to the sample disk, the lower end of the first rotating shaft passes sequentially through the shaft center of the second synchronous pulley and the shaft center of the second bearing and is driven to rotate by the second synchronous pulley; the output shaft of the first motor rotates, driving the first synchronous pulley to rotate, which in turn drives the second synchronous pulley to rotate synchronously via the first synchronous belt, and the second synchronous pulley drives the first rotating shaft to rotate, thereby driving the sample disk and the reaction disk to rotate.

[0015] In an optional embodiment, the second drive assembly further includes a second motor, a third synchronous pulley, a fourth synchronous pulley, a second synchronous belt, a second bearing bracket, a third bearing, a fourth bearing, and a bushing; the robotic arm includes a second rotating shaft and a cantilever, the upper end of the second rotating shaft is mounted on the liquid injection needle via the cantilever, the outer wall of the second rotating shaft is provided with a vertical groove along its axial direction, the height of the vertical groove being the same as the lifting height of the liquid injection needle; the second motor is mounted below the reagent tray; the third synchronous pulley is sleeved on the output shaft of the second motor; the fourth synchronous pulley is arranged side by side with the third synchronous pulley and is connected by the second synchronous belt; the fourth synchronous pulley is provided with a fastening groove along its radial direction, the fastening groove containing steel balls and elastic fasteners; the third bearing is mounted on the second bearing bracket and located above the fourth synchronous pulley; the fourth bearing is mounted on the... The second bearing is mounted on the second bearing bracket and located below the fourth synchronous pulley; the second rotating shaft is fitted with the bushing, and the second rotating shaft and the bushing are mounted on the second bearing bracket through the third bearing and the fourth bearing. The upper end of the second rotating shaft and the bushing pass through the axis of the third bearing and the shaft hole of the reagent tray in sequence and extend upward. The lower end of the second rotating shaft passes through the axis of the fourth synchronous pulley and the axis of the fourth bearing in sequence and is driven to rotate by the fourth synchronous pulley; the bushing is provided with a radial through hole; the elastic fastener passes through the radial through hole and pushes the steel ball, pressing the steel ball tightly into the vertical slide groove of the second rotating shaft; the output shaft of the second motor rotates, driving the third synchronous pulley to rotate, and driving the fourth synchronous pulley to rotate synchronously through the second synchronous belt. The fourth synchronous pulley drives the second rotating shaft, the bushing, the second rotating shaft, and the liquid injection needle to rotate.

[0016] In an optional embodiment, the second drive assembly further includes a lifting unit, which includes a connecting block, a lifting motor, a slider, and a guide rail. The connecting block has a first connecting hole and a second connecting hole arranged side-by-side. The lifting motor is mounted above the connecting block, and its lead screw extends downward and passes through the second connecting hole, being fixedly installed with the connecting block. The guide rail is installed parallel to the lead screw of the lifting motor. One side of the slider is slidably installed with the guide rail, and the other side is fixedly installed with the connecting block. The lower end of the second rotating shaft passes through the first connecting hole and is fixedly installed with the connecting block through the first connecting hole. When the lead screw of the lifting motor moves up and down, it drives the slider to slide on the guide rail, and drives the connecting block, the second rotating shaft, and the liquid injection needle to rise and fall. The vertical groove of the second rotating shaft and the steel ball generate relative movement.

[0017] In an optional embodiment, the reagent tray is further provided with a needle washing position, which is adjacent to the reagent position and is used to install a cleaning device.

[0018] In an optional embodiment, the delivery device includes a first valve, a buffer zone, a second valve, and a plunger pump connected in sequence; the first valve is connected to the injection needle via a pipe; when the liquid sample is drawn, the plunger pump operates to provide suction, drawing the liquid sample into the buffer zone through the second valve, the first valve, and the injection needle; when the liquid sample is injected, the plunger pump operates to provide thrust, releasing the liquid sample stored in the buffer zone through the second valve, the first valve, and the injection needle.

[0019] The sample preparation mechanism for pesticide residue detection of the present invention has the following beneficial effects: Compared to traditional pesticide residue testing methods, the technical solution in this embodiment automatically realizes the functions of sample preparation, sample transfer, reagent (reaction reagent, colorimetric reagent, substrate, etc.) or enzyme addition, mixing of the test solution, and substrate addition through the cooperation of the sample storage and transportation device. It no longer relies on professional technicians to prepare the test sample, and has a reasonable structural design, small footprint and volume, stronger convenience and environmental adaptability, and low cost of pesticide residue testing.

[0020] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and are not intended to limit the invention.

[0021] To better understand and implement this invention, the following detailed description is provided in conjunction with the accompanying drawings. Attached Figure Description

[0022] Figure 1 A perspective view of the intelligent pesticide residue detection device provided in an embodiment of the present invention; Figure 2 This is a schematic diagram of the internal structure of the intelligent pesticide residue detection device provided in an embodiment of the present invention; Figure 3 This is a schematic diagram of the sample tray provided in an embodiment of the present invention; Figure 4 A schematic diagram of the installation of the first driving component provided in an embodiment of the present invention; Figure 5 A schematic diagram of the installation of the reaction disk provided in an embodiment of the present invention; Figure 6 An installation diagram of the first storage module provided in an embodiment of the present invention; Figure 7 This is a schematic diagram of the sample tray provided in an embodiment of the present invention; Figure 8 A schematic diagram of the structure of the reaction disk provided in this embodiment of the invention; Figure 9 This is a schematic diagram of the structure of the cuvette receiving tank provided in an embodiment of the present invention; Figure 10 A schematic diagram of the driving structure of the second rotating shaft provided in an embodiment of the present invention; Figure 11 This is a schematic diagram of the structure of the second storage module provided in an embodiment of the present invention; Figure 12 A cross-sectional schematic diagram of the second storage module and the liquid sample transfer module provided in an embodiment of the present invention; Figure 13 This is a schematic diagram of the installation of reagent bottle in-situ detection according to an embodiment of the present invention; Figure 14 This is a schematic diagram of the installation between the second rotating shaft, the bushing, and the fourth synchronous pulley provided in an embodiment of the present invention; Figure 15 This is a schematic diagram illustrating the working principle of the conveying device provided in an embodiment of the present invention.

[0023] Icon labels: 1. Housing; 11. Frame; 12. Base; 2. Liquid sample storage and retrieval device; 21. First storage module; 211. Sample tray; 2111. Sample position; 2112. Sample application window; 2113. Contact platform; 2114. Recess; 2115. Mounting platform; 212. Reaction tray; 2121. Partition; 2122. Protrusion; 2123. Cuvette position; 2124. Detection port; 2125. Silicone sleeve; 213. First drive assembly; 2131. First motor; 2132. First synchronous pulley; 2133. 2134. Second synchronous pulley; 2135. First synchronous belt; 2136. First rotating shaft; 2137. Snap-fit ​​part; 2138. First bearing; 2139. Second bearing; 210. Sample receiving slot; 211. Cuvette receiving slot; 22. Second storage module; 221. Reagent tray; 2211. Reagent position; 2212. In-situ detection slot; 23. Liquid sample transfer module; 231. Robotic arm; 2311. Second rotating shaft; 23111. Vertical slide; 232. Liquid dispensing needle; 233. Second drive assembly; 2331 1. Second motor; 2332. Third synchronous pulley; 2333. Fourth synchronous pulley; 23331. Fastening groove; 2334. Second synchronous belt; 2336. Third bearing; 2337. Fourth bearing; 2338. Bushing; 2335. Lifting unit; 23351. Connecting block; 23352. Lifting motor; 23353. Sliding block; 23354. Guide rail; 3. Detection device; 31. Spectrophotometer; 32. Photoelectric sensor; 4. Result output device; 5. Enzyme storage device; 51. Storage box; 52. 53. Enzyme storage bottle; 54. Semiconductor cooling chip; 55. Heat sink; 56. Temperature sensor; 77. Cleaning device; 78. First support; 79. First upright plate; 70. First motor mounting plate; 71. First bearing bracket; 72. First detection mounting bracket; 73. Second detection mounting bracket; 74. Second support; 75. Second upright plate; 76. Second motor mounting plate; 77. Second bearing bracket; 88. Clear water bottle; 89. Buffer solution bottle; 80. Wastewater bottle; 200. Sample bottle; 300. Cuvette. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The components of the embodiments of the present invention described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0025] Therefore, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the invention without inventive effort are within the scope of protection of the invention.

[0026] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0027] This invention discloses a sample preparation mechanism for pesticide residue detection, applicable to diverse scenarios such as agricultural product quality supervision and inspection, disease prevention and control, environmental protection, industrial and commercial administration, and vegetable wholesale markets. It automates the steps of sample transfer, reagent injection, sample mixing, enzyme addition, substrate addition, and quantitative pesticide residue detection. This sample preparation mechanism is suitable for detecting organophosphates, carbamates, and other pesticide residues.

[0028] The sample preparation mechanism for pesticide residue detection includes: a main control device (not shown in the figure), a sample storage and retrieval device electrically connected to the main control device, and a conveying device.

[0029] To improve the portability, ease of use, and environmental adaptability of the sample preparation mechanism for pesticide residue detection, the sample preparation mechanism can be housed in a box 1. The box 1 includes a top cover (not shown), a frame 11, and a base 12, which are installed together from top to bottom to form the box 1.

[0030] In this embodiment, the sample preparation mechanism for the test liquid is an important component of the intelligent pesticide residue detection device. The intelligent pesticide residue detection device also includes a detection device and a result output device installed inside the chamber.

[0031] A communication connection is established between the main control device of the sample preparation mechanism and the sample storage and retrieval device 2, the conveying device, the detection device 3, and the result output device 4. This communication connection can be wireless or wired to achieve real-time interaction during pesticide residue detection. For example, the sample storage and retrieval device 2, the conveying device, the detection device 3, and the result output device 4 can receive control signals sent by the main control device or send detection signals back to the main control device. In this embodiment, the form of the main control device is not limited. It can be a control board specifically designed for controlling each stage of pesticide residue detection, or other intelligent devices such as general-purpose computers, smart tablets, smartphones, smart bracelets, industrial control computers, etc., or even a cloud server or other virtual computer. In this embodiment, the main control device is a control board located inside the housing 1.

[0032] Combination Figures 2 to 6 The liquid sample storage and retrieval device 2 includes a first storage module 21, a second storage module 22, and a liquid sample transfer module 23. The first storage module 21 includes a sample tray 211, a reaction tray 212, and a first driving assembly 213. The sample tray 211 has multiple sample positions 2111 and multiple sample application windows 2112. The sample positions 2111 are used to place sample vials 200, specifically 18 sample positions 2111. The reaction tray 212 is installed below and fixed to the sample tray 211, and can be rotated by the sample tray 211. The reaction tray 212 has multiple cuvette positions 2123, which are used to place cuvettes 300. Each cuvette position 2123 corresponds to a sample application window 2112, specifically 18 cuvette positions 2123 and 18 sample application windows 2112. Liquid samples are injected into the cuvettes 300 through the sample application windows 2112. The reaction disk 212 is equipped with multiple cuvettes 300, which can realize multi-channel synchronous detection, acquire multi-channel detection data, and improve detection efficiency and detection accuracy.

[0033] Combination Figure 7 , Figure 8 As can be seen, both the reaction disk 212 and the sample disk 211 are provided with screw holes. The reaction disk 212 and the sample disk 211 can be fixedly installed by screws, or they can be fixedly connected by a mutually mating structure. Optionally, the sample disk 211 has a number of recesses 2114 on the side near the reaction disk 212, and the reaction disk 212 has a number of protrusions 2122 on the side near the sample disk 211. The shape of the protrusions 2122 corresponds to the shape of the recesses 2114, so that the reaction disk 212 and the sample disk 211 can be installed together and securely.

[0034] Optional, such as Figure 5 , Figure 8As shown, each pair of cuvette positions 2123 in the reaction disk 212 is separated by a partition 2121, and a protrusion 2122 is provided on the top of each partition 2121; the shape of the recess 2114 and the protrusion 2122 can be triangular, and a silicone sleeve 2125 is fitted on the protrusion 2122 to make the protrusion 2122 and the recess 2114 fit more tightly.

[0035] Combination Figures 2 to 6 The first driving component 213 is located below and fixedly connected to the sample tray 211. The first driving component 213 drives the sample tray 211 to rotate, causing the sample vials 200 on it to rotate as well. This rotates the sample vials 200 to a suitable position, facilitating the addition of buffer solutions, reagents (reaction reagents, chromogenic agents, substrates, etc.), and enzymes. It also facilitates the transfer of the test solution to the cuvette 300. Simultaneously, the rotation of the sample tray 211 also helps to evenly mix the liquid sample in the sample vials 200 at various stages. The rotation of the sample tray 211 also drives the reaction tray 212 below it to rotate, causing the cuvette 300 on the reaction tray 212 to rotate as well. This facilitates the transfer of the liquid sample soaked in the test substance to the cuvette 300, the addition of chromogenic agents or enzymes, or the cleaning of the cuvette 300.

[0036] like Figures 9 to 12 As shown, the second storage module 22 includes a reagent tray 221 with multiple reagent positions 2211. The reagent positions 2211 are used to store enzymes or reagents. The center of the reagent tray 221 has a shaft hole for mounting a robotic arm 231. The robotic arm 231 can drive the dispensing needle 232 to rotate and reach the reagent position of the reagent tray 221.

[0037] To ensure secure installation of the reagent bottles, each reagent position 2211 is a circular hole, with a bottle sleeve installed below it. The upper end of the bottle sleeve passes through the circular hole and is secured with screws. The bottle sleeve has a cylindrical receiving cavity for holding the reagent bottle or test tube.

[0038] Optional, such as Figure 12 As shown, in order to realize in-situ detection of reagent bottles, the reagent tray 221 is provided with an in-situ detection slot 2212 facing each reagent position 2211 on the side near the base. A photoelectric sensor 32 is installed in the in-situ detection slot 2212 for in-situ detection of reagent bottles, test tubes or enzyme storage bottles 52 in each reagent position 2211.

[0039] The liquid sample transfer module 23 includes a robotic arm 231, a dispensing needle 232, and a second drive assembly 233. The dispensing needle 232 is mounted on the first end of the robotic arm 231 and is used to extract and release liquid samples. The first end of the robotic arm 231 passes through the shaft hole of the reagent tray 221, so that the dispensing needle 232 is positioned above the reagent position 2211. The second drive assembly 233 is mounted below the reagent tray 221 and connected to the second end of the robotic arm 231. The second drive assembly 233 is used to drive the robotic arm 231 to lift or rotate, thereby driving the dispensing needle 232 to lift or rotate. Specifically, the robotic arm 231 drives the dispensing needle 232 to rotate around the shaft hole of the reagent tray 221 with the horizontal distance between the shaft hole and the dispensing needle 232 as the radius, that is, the dispensing needle 232 moves along the circumference of a preset circle.

[0040] The delivery device is connected to the liquid injection needle 232 through a pipe, and is used to drive the liquid injection needle 232 to draw or release liquid samples at each sample position 2111, each cuvette position 2123, and each reagent position 2211.

[0041] The first driving component 213 drives the sample disk 211 and the reaction disk 212 to rotate. The second driving component 233 drives the robotic arm 231 to move the liquid dispensing needle 232 up and down in a timely manner, and drives the robotic arm 231 to rotate the liquid dispensing needle 232 along the circumference of a preset circle so that the liquid dispensing needle 232 reaches each sample position 2111, each cuvette position 2123, and each reagent position 2211 to extract or release liquid samples.

[0042] Compared to traditional pesticide residue testing methods, the technical solution in this embodiment automatically realizes the functional steps of liquid sample preparation, liquid sample transfer, addition of reagents (reaction reagents, colorimetric reagents, substrates, etc.) or enzymes, mixing of the test solution, and addition of substrates through the cooperation of liquid sample storage and transportation devices. It has a high degree of intelligence, high efficiency in liquid sample preparation, no longer relies on professional technicians to prepare test liquid samples, and has a reasonable structural design, small footprint and volume, stronger convenience and environmental adaptability, and low cost of pesticide residue testing.

[0043] The following is a detailed description of each device in the sample preparation mechanism for pesticide residue detection.

[0044] To save space in the chamber, a clever structural design was implemented, taking into account the required number of detection channels and the amount of reagents to be added.

[0045] In this embodiment, Figures 2-8As shown, the sample tray 211 is a circular disk that can be driven to rotate. Multiple sample positions 2111 are arranged in a ring around the edge of the disk, specifically 18 equidistant sample positions 2111. When the sample vial 200 is placed in the sample position 2111, its opening faces upwards. Multiple sample application windows 2112 are arranged in a ring around the inner side of the sample positions 2111 on the sample tray 211, specifically 18 equidistant sample application windows 2112. Optionally, a first decorative sticker is affixed to the upper surface of the sample tray 211 facing the cover, and the first decorative sticker is marked with the sample position 2111 number and / or the cuvette position 2123 number.

[0046] like Figure 2 , Figure 9 , Figure 12 As shown, the reagent tray 221 is crescent-shaped and is arranged side-by-side with the sample tray 211. The reagent tray 221 is non-rotatable, while the sample tray 211 is rotatable. Multiple reagent positions 2211 are arranged in an arc shape on the side of the reagent tray 221 away from the sample tray 211. These positions are equidistant along the arc, making it easier for the dispensing needle 232 to be controlled and transferred to each reagent position 2211. Optionally, the reagent tray 221 has five reagent positions 2211, one of which is used for enzyme storage. The side of the reagent tray 221 closest to the sample tray 211 has an arc-shaped portion concave towards its center. The outer edge of the sample tray 211 mates with the arc-shaped portion of the reagent tray 221, with a small gap between them, allowing the sample tray 211 to rotate freely. This allows the robotic arm 231 to rotate and move the dispensing needle 232 to each sample vial 200, cuvette 300, or reagent vial to aspirate or release liquid samples. Optionally, a second decorative sticker is affixed to the upper surface of the reagent tray 221 facing the cover, the second decorative sticker being marked with the reagent slot 2211 number, the cleaning tank label, and / or the enzyme storage label.

[0047] Specifically, when the robotic arm 231 rotates the dispensing needle 232 on the reagent tray 221, the dispensing needle 232 reaches each reagent position 2211 to draw reagents; the robotic arm 231 rotates the dispensing needle 232 to a first preset position outside the reagent tray 221 ( Figure 2 When the sample is at sample position 3, the sample tray 211 is driven to rotate, thereby moving the sample vials 200 on the sample position 2111 to below the liquid injection needle 232 in sequence, so as to realize liquid sample injection or extraction; the robotic arm 231 drives the liquid injection needle 232 to rotate to the second preset position outside the reagent tray 221. Figure 2 When the sample plate 211 is rotated (at cuvette position 2123), the sample plate 211 is driven to rotate, which in turn drives the reaction plate 212 to rotate, thereby moving the cuvette 300 on cuvette position 2123 to the bottom of the injection needle 232 to realize liquid sample injection or extraction. Among them, each reagent position 2211, the first preset position, and the second preset position are all on the circumference of the same preset circle.

[0048] Meanwhile, the outer edge of the sample tray 211 is tightly fitted with the arc-shaped part of the reagent tray 221, which can reduce the radius of rotation of the robotic arm 231, reduce the platform area, thereby reducing the volume of the box 1, saving space, making it easy to carry and transport, and reducing the requirements for the testing environment.

[0049] When the sample vials 200 and cuvettes 300 are rotated, they are prone to shaking, which can lead to interruption of the detection process if the shaking is severe. To prevent the sample vials 200 and cuvettes 300 from shaking during rotation and to improve the structural stability of the equipment, a corresponding stable structure is required to accommodate the sample vials 200 and cuvettes 300. Preferably, the outer circumferential edge of the sample tray 211 is provided with an abutment platform 2113. The first storage module 21 also includes a sample receiving groove 214 and a cuvette receiving groove 215. The sample receiving groove 214 is located below the sample tray 211, is non-rotatable, and the top of its outer ring sidewall abuts against the abutment platform 2113 on the outer circumferential edge of the sample tray 211. The sample receiving groove 214 has a first annular space; the opening of the first annular space faces each sample position 2111, which is used to accommodate and provide rotation space for the sample vials 200. The cuvette receiving tank 215 is located below the reaction plate 212 and inside the sample receiving tank 214. It is non-rotatable and has a second annular space. The opening of the second annular space faces each cuvette position 2123 and is used to accommodate and provide rotation space for the cuvette 300. An annular heating plate is provided on the outer wall of the cuvette receiving tank 215 to maintain the reaction temperature of the test solution in the cuvette 300 and provide constant temperature reaction conditions to ensure enzyme activity.

[0050] Optionally, in order to achieve spectrophotometric detection, pesticide residue testing equipment, such as... Figure 8 As shown, the reaction disk 212 has multiple detection ports 2124 arranged radially therefrom, each detection port 2124 corresponding to a cuvette position 2123. A first detection window 2151 is provided on the outer wall of the cuvette receiving tank 215, and a second detection window 2152 is provided on its inner wall. The first detection window 2151 and the second detection window 2152 are located on opposite sides of the cuvette position 2123, respectively. During detection, the sample disk 211 needs to be driven to rotate, which in turn drives the reaction disk 212 and the cuvette 300 on it to rotate, so that the cuvette 300 containing the test liquid is aligned with the first detection window 2151 and the second detection window 2152, facilitating spectrophotometric detection.

[0051] From the perspective of pesticide residue testing, such as Figure 6 , Figure 9As shown, a third detection window 2153 is provided on the outer wall of the cuvette container 215. The spectrophotometer 31 of the detection device 3 includes an emitting end and a receiving end. The emitting end, cuvette 300, and receiving end are arranged in sequence along the radial direction of the reaction plate 212. The light signal emitted by the emitting end irradiates the test liquid in the cuvette 300 through the third detection window 2153 of the cuvette container 215 and the detection port 2124 of the reaction plate 212. The receiving end receives the detection signal through the detection port 2124 of the reaction plate 212 and the second detection window of the cuvette container 215.

[0052] To enable in-situ detection of cuvette 300, the detection device 3 may optionally include a photoelectric sensor 32 for in-situ detection of cuvette 300; the photoelectric sensor 32 passes through the second detection window and the detection port 2124 of the reaction plate 212 to perform in-situ detection of cuvette 300.

[0053] To achieve the rotation of the sample disk 211 and the reaction disk 212, this embodiment is achieved through the following structure.

[0054] The sample tray 211 has a shaft hole at its center; the first drive assembly 213 includes a first motor 2131, a first synchronous pulley 2132, a second synchronous pulley 2133, a first synchronous belt 2134, a first bearing 2136 frame, a first bearing 2136, a second bearing 2137, and a first rotating shaft 2135.

[0055] The first motor 2131 is mounted below the sample tray 211; the first synchronous pulley 2132 is fitted onto the output shaft of the first motor 2131; the second synchronous pulley 2133 is arranged side by side with the first synchronous pulley 2132 and is connected by a first synchronous belt 2134; the first bearing 2136 is mounted on the first bearing 2136 frame and is located above the second synchronous pulley 2133; the second bearing 2137 is mounted on the first bearing 2136 frame and is located below the second synchronous pulley 2133.

[0056] The first rotating shaft 2135 is mounted on the first bearing 2136 frame via a first bearing 2136 and a second bearing 2137. The upper end of the first rotating shaft 2135 passes sequentially through the shaft center of the first bearing 2136 and the shaft hole of the sample tray 211, and is fixedly connected to the sample tray 211. The lower end of the first rotating shaft 2135 passes sequentially through the shaft center of the second synchronous pulley 2133 and the shaft center of the second bearing 2137, and is driven to rotate by the second synchronous pulley 2133. Optionally, the shaft hole of the sample tray 211 is a limiting shaft hole, and a mounting platform 2115 is provided at the limiting shaft hole. The upper end of the first rotating shaft 2135 is provided with a snap-fit ​​part 21351 that matches the mounting platform 2115. The upper end of the first rotating shaft 2135 is provided with a screw hole for installing a cap.

[0057] The output shaft of the first motor 2131 rotates, driving the first synchronous pulley 2132 to rotate. The first synchronous belt 2134 drives the second synchronous pulley 2133 to rotate synchronously. The second synchronous pulley 2133 drives the first rotating shaft 2135 to rotate, thereby driving the sample disk 211 and the reaction disk 212 to rotate.

[0058] To achieve the rotation of the robotic arm 231 and the liquid injection needle 232, this embodiment is achieved through the following structure.

[0059] The second drive assembly 233 also includes a second motor 2331, a third synchronous pulley 2332, a fourth synchronous pulley 2333, a second synchronous belt 2334, a second bearing 2137 frame, a third bearing 2336, a fourth bearing 2337, and a bushing 2338.

[0060] The robotic arm 231 includes a second rotating shaft 2311 and a cantilever. A dispensing needle 232 is mounted on the upper end of the second rotating shaft 2311 via the cantilever. A vertical groove 23111 is provided along the axial direction of the outer wall of the second rotating shaft 2311, and the height of the vertical groove 23111 is the same as the lifting height of the dispensing needle 232. The length of the cantilever is equal to the radius of a preset circle, with the shaft hole of the reagent tray 221 as its center and the horizontal distance between the shaft hole and the dispensing needle 232 as its radius.

[0061] The second motor 2331 is mounted below the reagent tray 221 via the second motor mounting plate 722; the third synchronous pulley 2332 is fitted onto the output shaft of the second motor 2331; the fourth synchronous pulley 2333 is arranged side by side with the third synchronous pulley 2332 and is connected by a second synchronous belt 2334. Figure 14 As shown, the fourth synchronous pulley 2333 has a fastening groove 23331 arranged radially therein, and steel balls 23332 and elastic fasteners (not shown in the figure) are arranged in the fastening groove 23331. The third bearing 2336 is mounted on the second bearing 2137 frame and is located above the fourth synchronous pulley 2333; the fourth bearing 2337 is mounted on the second bearing 2137 frame and is located below the fourth synchronous pulley 2333; the second rotating shaft 2311 is fitted with a bushing 2338, and the second rotating shaft 2311 and bushing 2338 are mounted on the second bearing 2137 frame through the third bearing 2336 and the fourth bearing 2337. The upper end of the second rotating shaft 2311 and the bushing 2338 pass through the shaft of the third bearing 2336 and the shaft hole of the reagent tray 221 in sequence and extend upward. The lower end of the second rotating shaft 2311 passes through the shaft of the fourth synchronous pulley 2333 and the shaft of the fourth bearing 2337 in sequence and is driven to rotate by the fourth synchronous pulley 2333.

[0062] like Figure 14As shown, in order to eliminate the rotational clearance between the second rotating shaft 2311 and the bushing 2338, the bushing 2338 is provided with a radial through hole (which is blocked by the fourth synchronous pulley 2333); the elastic fastener passes through the radial through hole and pushes the steel ball 23332, which is pressed tightly against the vertical slide groove 23111 of the second rotating shaft 2311, so that the second rotating shaft 2311 can be driven to rotate or lift.

[0063] The output shaft of the second motor 2331 rotates, driving the third synchronous pulley 2332 to rotate. The second synchronous belt 2334 drives the fourth synchronous pulley 2333 to rotate synchronously. The fourth synchronous pulley 2333 drives the second rotating shaft 2311, the bushing 2338, the second rotating shaft 2311, and the liquid injection needle 232 to rotate.

[0064] To achieve the lifting and lowering of the robotic arm 231, such as Figure 11 , Figure 12 As shown, the second drive assembly 233 also includes a lifting unit 2335, which includes a connecting block 23351, a lifting motor 23352, a slider 23353, and a guide rail 23354. Therefore, the connecting block 23351 has a first connecting hole and a second connecting hole arranged side by side; the lifting motor 23352 is installed above the connecting block 23351, and the lead screw of the lifting motor 23352 extends downward and passes through the second connecting hole, and is fixedly connected to the connecting block 23351 through the second connecting hole; the guide rail 23354 is installed parallel to the lead screw of the lifting motor 23352; one side of the slider 23353 is slidably installed with the guide rail 23354, and the other side is fixedly installed with the connecting block 23351; the lower end of the second rotating shaft 2311 is installed through the first connecting hole; when the lead screw of the lifting motor 23352 moves up and down, it drives the slider 23353 to slide on the guide rail 23354, and drives the connecting block 23351, the second rotating shaft 2311 and the liquid injection needle 232 to rise and fall, and the vertical groove of the second rotating shaft 2311 and the steel ball generate relative movement.

[0065] To achieve cleaning of the injection needle 232, such as Figure 10As shown, the sample preparation mechanism for the test liquid is also equipped with a cleaning device 6; a needle washing position is also provided on the reagent tray 221, which is adjacent to the reagent position 2211 and located on the circumference of a preset circle; the cleaning device 6 is installed at the needle washing position, and the cleaning device 6 includes a cleaning tank located at the center of the needle washing position, with a water inlet at the bottom of the cleaning tank; the water inlet is connected to a clean water bottle through a pipe and a conveying device, and a water outlet is provided on the outer periphery of the cleaning tank, with a water outlet at the bottom of the water outlet, which is connected to a waste water bottle through a pipe and a conveying device. The liquid addition needle 232 is driven to move above the cleaning tank and is controlled to descend into the cleaning tank. Clean water at the bottom water inlet rinses the liquid addition needle 232, while waste water flows out from the bottom water outlet and is transferred to the waste water bottle by the conveying device. To facilitate the rotation control of the liquid addition needle 232, the needle washing position and each reagent position 2211 are installed at equal intervals along an arc.

[0066] To facilitate convenient enzyme storage, the sample preparation mechanism also includes an enzyme storage device 5. The enzyme storage device 5 comprises a storage box 51, an enzyme storage bottle 52, a thermoelectric cooler 53, a heat sink 54, and a temperature sensor (not shown). The storage box 51 is located below the reagent tray 221, and its bottom has a temperature measuring hole (not shown). An installation window is provided on one side wall of the storage box 51. The thermoelectric cooler 53 is installed inside the installation window. The enzyme storage bottle 52 is placed inside the storage box 51 and corresponds to one of the reagent positions 2211 on the reagent tray 221. The heat sink 54 is installed on the outside of the storage box 51 and adjacent to the thermoelectric cooler 53. The temperature sensor is located inside the temperature measuring hole and is used to detect the temperature of the thermoelectric cooler 53.

[0067] To facilitate the delivery of liquid samples, the sample preparation mechanism also includes a delivery device for extracting, transferring, and releasing water, liquid samples, reagents, and enzymes.

[0068] The delivery device includes a first valve (valve No. 3), a buffer zone, a second valve (valve No. 4), and a plunger pump connected in sequence; both the first and second valves are three-way valves. The first valve's first channel is connected to the injection needle 232 via a pipe, its second channel is connected to the first waste liquid motor via a pipe, and its third channel is connected to the buffer zone via a pipe. The second valve's first channel is connected to the buffer zone via a pipe, its second channel is connected to the plunger pump, and its third channel is connected to a peristaltic pump via a pipe. During sample extraction, the plunger pump provides suction, drawing the sample into the buffer zone through the second valve, the first valve, and the injection needle 232; during sample release, the plunger pump provides thrust, releasing the sample stored in the buffer zone through the second valve, the first valve, and the injection needle 232. During sample extraction and transfer, the injection needle 232 needs to rotate, and the sample tray 211 and reaction tray 212 also need to rotate.

[0069] To prevent the plunger pump from dry running and affecting its service life, water needs to be drawn into the plunger pump each time it is started. Optionally, the conveying device also includes a clean water valve and a third valve (valve No. 5), both of which are three-way valves. The first channel of the third valve is connected to the first channel of the clean water valve via a pipe, its second channel is connected to the plunger pump, and its third channel is suspended.

[0070] The plunger pump's inlet is connected to the water bottle via a pipe, a third valve, and a clean water valve. When the plunger pump draws water, the second channel connecting the third valve to the plunger pump opens, and the third channel connecting the clean water valve to the clean water bottle opens, causing the plunger pump to operate and draw water through the third valve and the clean water valve.

[0071] During the entire pesticide residue testing process, the dosing needle 232 needs to be cleaned. Optionally, the conveying device also includes a clean water motor, a first waste liquid motor, and a waste liquid valve (a three-way valve). The first channel of the clean water valve is connected to the clean water motor, its second channel is connected to an external clean water source via a pipe, and its third channel is connected to a clean water bottle via a pipe. The first channel of the waste liquid valve is connected to the first waste liquid motor, its second channel is connected to an external waste liquid pool, and its third channel is connected to a waste liquid bottle. When the clean water motor operates, it delivers clean water to the dosing needle 232 cleaning tank through the clean water valve to rinse the dosing needle 232; simultaneously, the first waste liquid motor operates, transferring waste liquid to the waste liquid bottle through the waste liquid valve.

[0072] During the preparation of the test liquid sample, the cuvette 300 needs to be cleaned. Optionally, the delivery device also includes a second waste liquid motor, one end of which is connected to the second channel of the first valve through a pipe, and the other end is connected to the first channel of the waste liquid valve through a pipe. During the cleaning of the cuvette 300, water is drawn up by the dosing needle 232 and transferred to the cuvette 300 for cleaning. Specifically, when the dosing needle 232 draws up water, it controls the connection between the first and third channels of the water valve, the connection between the first and second channels of the third valve, the connection between the first and second channels of the second valve, and the connection between the first and third channels of the first valve to form a water suction channel. The plunger pump is controlled to operate to draw water into the buffer zone, and the dosing needle 232 is controlled to transfer the water to the cuvette 300. The plunger pump then operates again to release the water from the buffer zone into the cuvette 300 for cleaning. After cleaning, control the second waste liquid motor to run, control the first and second channels of the first valve to connect, and control the first and third channels of the waste liquid valve to connect, so as to transfer the waste liquid in the cuvette 300 to the waste liquid bottle.

[0073] When preparing the test solution, the buffer solution needs to be transferred to sample vial 200 first. To facilitate the addition of the buffer solution, the delivery device also includes a peristaltic pump, a buffer valve, a fourth valve (valve 1), and a fifth valve (valve 2). The buffer valve, the fourth valve (valve 1), and the fifth valve are all three-way valves.

[0074] The first and third channels of the buffer solution valve are connected, linking the buffer solution valve to the buffer solution bottle. The first and second channels of the fifth valve are also connected. The first and third channels of the fourth valve are connected. The first and third channels of the second valve are also connected, simultaneously controlling the peristaltic pump to draw buffer solution into the buffer zone. When the dosing needle 232 transfers the buffer solution to the sample bottle 200, the plunger pump is activated to release the buffer solution from the buffer zone into the sample bottle 200. Optionally, some air may be present in the buffer zone. Therefore, the air in the buffer zone needs to be purged before transferring the buffer solution. In this case, the first and third channels of the second valve and the first and second channels of the fourth valve are connected, with the second channel of the fourth valve opening to the outside. This activates the peristaltic pump, allowing the air in the buffer zone to be expelled through the second valve, the peristaltic pump, and the fourth valve.

[0075] In an optional embodiment, such as Figure 1 , Figure 2 As shown, the base inside the housing 1 of the intelligent pesticide residue detection device is equipped with a first bracket 71, a second bracket 72 and a third bracket (covered by the housing 1). The first bracket 71 is used to install and support the first storage module 21 and the detection device 3. The second bracket 72 is used to install and support the second storage module 22 and the liquid sample transfer module 23. The third bracket is used to install and support the clear water bottle 81, the buffer solution bottle 82 and the waste water bottle 83.

[0076] The first support 71 is located in the middle of the base and includes two first upright plates 711, a first motor mounting plate 2131, a first bearing 2136 bracket, a first inspection mounting bracket 714, and a second inspection mounting bracket 715. The two first upright plates 711 are mounted vertically side-by-side on the base, and the first motor mounting plate 2131 is mounted on and supported by the two first upright plates 711. The first motor mounting plate 2131 has a motor mounting position and a bearing mounting position. The motor mounting position is used to mount the first motor 2131, and the bearing mounting position is used to mount the bearing and the shaft. The first motor 2131 is mounted below the first motor mounting plate 2131, and its output shaft passes through the motor mounting position and is fixedly mounted to the first synchronous pulley 2132. The second synchronous pulley 2133 is mounted above the first motor mounting plate 2131 and corresponds to the bearing mounting position.

[0077] The first bearing 2136 frame is fixedly installed on the first motor mounting plate 2131, and the shaft hole on the first bearing 2136 frame corresponds to the bearing mounting position. The first bearing 2136 frame includes a first extension frame and a second extension frame arranged vertically, and the first extension frame and the second extension frame are used to install the first bearing 2136 and the second bearing 2137, respectively.

[0078] The first detection mounting bracket 714 is installed on the left side of the first motor mounting plate 2131. The side wall of the first detection mounting bracket 714 has a step that abuts against the edge of the motor mounting bracket and is used for screw fixing. The step of the first detection mounting bracket 714 also abuts against the second extension bracket of the first bearing 2136 bracket in the horizontal direction. The first detection mounting bracket 714 is provided with two vertical supports. The top of one vertical support is supported on the bottom of the cuvette receiving tank 215 and fixed to the cuvette receiving tank 215 with screws. The other vertical support is pressed against and supports the bottom of the sample receiving tank 214 and fixed to the sample receiving tank 214 with screws. Optionally, the vertical support used to support the cuvette receiving tank 215 is used to install a spectrophotometer 31. One side of the vertical support is used to install the transmitting end of the spectrophotometer 31, and the other side is used to install the receiving end of the spectrophotometer 31. The spectrophotometer 31 is installed in conjunction with the first and second detection windows so that the test liquid in the cuvette 300 can be detected by spectrophotometry through the two detection windows.

[0079] The second detection mounting bracket 715 is located on the right side of the first motor mounting plate 2131, and is fixed to the first bearing 2136 bracket by screws, and is arranged in the opposite direction to the first extension bracket and the second extension bracket. The photoelectric sensor 32 is mounted on the second detection mounting bracket 715 and faces the second detection window of the cuvette receiving slot 215, so that the cuvette 300 can be detected in place through the second detection window. Optionally, the second detection mounting bracket 715 is fixedly mounted to the sample receiving slot 214 by screws.

[0080] The second bracket 72 is mounted on the base, located to the left of the first bracket 71, and its structure is similar to that of the first bracket 71. Specifically, the second bracket 72 includes two second upright plates 721, a second motor mounting plate 722, and a second bearing 2137 bracket. The two second upright plates 721 are mounted vertically side by side on the base, and the second motor mounting plate 722 is mounted on and supported by the two second upright plates 721. The second motor mounting plate 722 is provided with a motor mounting position and a bearing mounting position. The motor mounting position is used to install the second motor 2331, and the bearing mounting position is used to install the bearing and the shaft. The second motor 2331 is mounted below the second motor mounting plate 722, and its output shaft passes through the motor mounting position and is fixedly mounted to the third synchronous pulley 2332. The third synchronous pulley 2332 is mounted above the second motor mounting plate 722 and corresponds to the bearing mounting position.

[0081] Optionally, the guide rail 23354 is vertically installed between the two second upright plates 721 and supported below the second motor mounting plate 722. When the lifting motor 23352 drives the lead screw and slider 23353 to slide, the slider 23353 and the guide rail 23354 move relative to each other.

[0082] The following is a detailed explanation of the steps for preparing the test liquid sample for pesticide residue testing.

[0083] The preparation of the test sample may include the following steps: S1: Clean the injection needle; S2: Add buffer solution to the sample vial containing the analyte through the injection needle; S3: Shake the analyte and buffer solution in the sample vial and allow it to settle for several minutes; S4: Clean the sampling needle, then draw the supernatant from the sample vial and transfer it to a cuvette; S5: Clean the injection needle, draw the enzyme, and add the enzyme to the cuvette; S6: Clean the injection needle, draw the colorimetric reagent, add it to the cuvette, and stabilize it for several minutes; S7: Clean the injection needle and add the substrate. Therefore, in the preparation of the test sample and the detection of pesticide residues, the steps requiring the coordinated rotation of the sample tray, reaction tray, and injection needle include at least S1 to S7.

[0084] Combination Figure 2 As you can see, when cleaning the liquid injection needle 232, the second motor 2331 drives the liquid injection needle 232 to rotate to the cleaning position through the second rotating shaft 2311. Clean water on the conveying device rinses the liquid injection needle 232. After cleaning, the liquid injection needle 232 is transferred to a suitable position.

[0085] When adding buffer solution, the buffer solution is first transferred to the buffer zone via a delivery device. Then, the first motor 2131 drives the first rotating shaft 2135 to rotate, causing the sample tray 211 to rotate. This brings the sample vials 200 requiring buffer solution to closer to the reagent tray 221. When the sample vials 200 requiring buffer solution and each reagent position 2211 are on the same preset circle, the sample tray 211 stops rotating. Then, the second motor 2331 drives the second rotating shaft 2311 to rotate, moving the dispensing needle 232 above the sample vials 200 requiring buffer solution. The buffer solution stored in the buffer zone is released via the delivery device and injected into the sample vials 200 through the dispensing needle 232. There are a total of 18 sample vials 200 on the sample tray 211. Rotating the sample tray 211 allows the sample vials 200 requiring buffer solution to sequentially approach the reagent tray 221, and the buffer solution is added using the above method. After the buffer solution is added, the first motor 2131 drives the first rotating shaft 2135 to rotate, which in turn rotates the sample tray 211 to mix the buffer solution and the analyte in the sample vial 200 to obtain the test sample solution.

[0086] After the sample solution to be tested is shaken and allowed to stand for several minutes, the liquid addition needle 232 is driven to rotate to the needle washing position for cleaning. Then, the liquid addition needle 232 is driven to rotate to the sample position 2111 to extract the sample solution to be tested from the sample bottle 200 and transfer it to the cuvette 300. Specifically, the second motor 2331 drives the second rotating shaft 2311 to rotate, thereby moving the liquid dispensing needle 232 to the first preset position, i.e., above the corresponding sample bottle 200. The lifting motor 23352 drives the liquid dispensing needle 232 to descend to the liquid surface of the sample bottle 200. The conveying device drives the liquid dispensing needle 232 to draw up the sample liquid to be tested and transfer it to the buffer zone. Then, the lifting motor 23352 drives the liquid dispensing needle 232 to rise above the sample bottle 200. The second motor 2331 drives the second rotating shaft 2311 to rotate, thereby moving the liquid dispensing needle 232 to the second preset position, i.e., above the corresponding cuvette 300. The lifting motor 23352 drives the liquid dispensing needle 232 to descend into the cuvette 300. The conveying device drives the release of the sample liquid to be tested from the buffer zone and injects it into the cuvette 300.

[0087] After the sample solution is transferred to cuvette 300, the dispensing needle 232 needs to be cleaned before enzyme can be drawn and added. Specifically, the dispensing needle 232 is driven to rotate to the needle washing position for cleaning, and then driven to rotate to the enzyme storage position. The lifting motor 23352 drives the dispensing needle 232 down into the enzyme storage bottle 52 to draw out the enzyme. Then, the lifting motor 23352 drives the dispensing needle 232 up away from the enzyme storage bottle 52. The second motor 2331 drives the second rotating shaft 2311 to rotate, moving the dispensing needle 232 to the second preset position, that is, above the corresponding cuvette 300. The lifting motor 23352 then drives the dispensing needle 232 down into the cuvette 300 to inject the enzyme. The chromogenic agent and substrate are added sequentially in the above manner, and finally, the detection device 3 performs spectrophotometric detection.

[0088] The sample preparation mechanism for pesticide residue detection of the present invention has the following beneficial effects: In the technical solution of this invention, the liquid sample preparation, liquid sample transfer, addition of reagents (reaction reagents, colorimetric reagents, substrates, etc.) or enzymes, mixing of the test solution, and addition of substrates are automatically realized through the cooperation of the liquid sample storage and retrieval device and the conveying device. It no longer relies on the professional operation of professional technicians to prepare the test liquid sample, uses fewer drive motor components, and has a reasonable structural design, small footprint and volume, stronger convenience and environmental adaptability, and low cost of pesticide residue detection.

[0089] Compared to the existing technology's design of two turntables and a rotating dispensing needle, the technical solution of this application only requires two drive motors to drive the rotation, resulting in a simpler control method and a more rational structure. The ingenious structural installation of the sample tray, reaction tray, reagent tray, and robotic arm in this application allows for sample transfer, reagent addition at each stage, and liquid sample transfer throughout the entire liquid sample preparation process using a single dispensing needle. Furthermore, multiple functions can be achieved simply by controlling the rotation of the sample tray, reaction tray, and robotic arm, simplifying the control method. This also reduces the footprint of the entire device, contributing to a smaller housing volume and improving the convenience and adaptability of the sample preparation mechanism for pesticide residue detection.

[0090] Note that the above description is merely a preferred embodiment of the present invention and the technical principles employed. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and various obvious changes, readjustments, and substitutions can be made without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in detail through the above embodiments, the present invention is not limited to the above embodiments, and may include many other equivalent embodiments without departing from the concept of the present invention, the scope of which is determined by the scope of the appended claims.

Claims

1. A sample preparation mechanism for pesticide residue detection, characterized in that, include: The main control device, and the liquid sample storage and retrieval device and the conveying device electrically connected to the main control device; The liquid sample storage and retrieval device includes a first storage module, a second storage module, and a liquid sample transfer module; The first storage module includes a sample tray, a reaction tray, and a first driving component; the sample tray is a circular disk with multiple sample positions distributed in a ring around its edge, the sample positions being used to place sample vials, and multiple sample dispensing windows distributed in a ring around the inner side of the sample positions on the sample tray. The first storage module further includes a sample receiving tank and a cuvette receiving tank; a contact platform is provided on the outer circumference of the sample tray; the sample receiving tank is located below the sample tray, and the top of its outer ring sidewall abuts against the contact platform of the sample tray; the sample receiving tank has a first annular space; the opening of the first annular space faces each of the sample positions, and is used to accommodate and provide rotation space for the sample bottles; the cuvette receiving tank is located below the reaction tray and inside the sample receiving tank, and has a second annular space; the opening of the second annular space faces each of the cuvette positions, and is used to accommodate and provide rotation space for the cuvettes; an annular heating plate is provided on the sidewall of the cuvette receiving tank to maintain the reaction temperature of the test liquid in the cuvette; The reaction plate is installed below the sample plate. The reaction plate has multiple cuvette positions for placing cuvettes. Each cuvette position corresponds to a sample dispensing window, through which liquid samples are injected into the cuvettes. The sample plate has several recesses on the side near the reaction plate and several protrusions on the side near the sample plate. The shapes of the protrusions correspond to the shapes of the recesses, allowing the reaction plate and sample plate to fit together. The first driving component is disposed below the sample tray and is fixedly connected to the sample tray. The first driving component is used to drive the sample tray to rotate, thereby causing the sample bottle on it to rotate. The rotation of the sample tray causes the reaction tray below it to rotate, thereby causing the cuvette on the reaction tray to rotate. The second storage module includes a reagent tray, which is crescent-shaped and arranged side-by-side with the sample tray. Multiple reagent positions are arranged in an arc on the side of the reagent tray away from the sample tray. These reagent positions are used to store enzymes or reagents. An arc-shaped portion concave towards the center of the reagent tray is provided on the side of the reagent tray closest to the sample tray. The outer circumference of the sample tray mates with the arc-shaped portion of the reagent tray, with a gap between them. A shaft hole is provided at the center of the reagent tray. The liquid sample transfer module includes a robotic arm, a dispensing needle, and a second drive assembly. The dispensing needle is mounted on the first end of the robotic arm and is used to extract and release liquid samples. The first end of the robotic arm passes through the shaft hole of the reagent tray, positioning the dispensing needle above the reagent position. The second drive assembly is mounted below the reagent tray and connected to the second end of the robotic arm. The second drive assembly is used to drive the robotic arm to lift or rotate, thereby causing the dispensing needle to lift or rotate. The robotic arm includes a second rotating shaft and a cantilever, with the dispensing needle mounted on the upper end of the second rotating shaft via the cantilever. The delivery device is connected to the liquid injection needle and is used to drive the liquid injection needle to draw or release liquid samples; The first driving component drives the sample plate and the reaction plate to rotate. The second driving component drives the robotic arm to move the liquid dispensing needle up and down in a timely manner, and drives the robotic arm to rotate the liquid dispensing needle in a timely manner, so that the liquid dispensing needle reaches each of the sample positions, each of the cuvette positions, and each of the reagent positions to extract or release liquid samples, thereby realizing the preparation of the liquid sample to be tested. When the robotic arm rotates the dispensing needle on the reagent tray, the dispensing needle reaches each of the reagent positions to draw reagents. When the robotic arm rotates the dispensing needle to a first preset position outside the reagent tray, the sample tray is driven to rotate, so that the sample bottles on the sample positions move sequentially to below the dispensing needle to realize liquid sample injection or extraction. When the robotic arm rotates the dispensing needle to a second preset position outside the reagent tray, the sample tray is driven to rotate, which drives the reaction tray to rotate, so that the cuvettes on the cuvette positions move sequentially to below the dispensing needle to realize liquid sample injection or extraction.

2. The sample preparation mechanism for pesticide residue detection according to claim 1, characterized in that, The reaction disk is provided with multiple detection ports along its radial direction, and each detection port corresponds to each cuvette position. The outer wall of the cuvette container is provided with a first detection window, and the inner wall is provided with a second detection window. The first detection window and the second detection window are respectively located on both sides of the cuvette position.

3. The sample preparation mechanism for pesticide residue detection according to claim 1, characterized in that, The sample disk has a shaft hole at its center; The first drive assembly includes a first motor, a first synchronous pulley, a second synchronous pulley, a first synchronous belt, a first bearing bracket, a first bearing, a second bearing, and a first rotating shaft; The first motor is mounted below the sample tray; the first synchronous pulley is sleeved on the output shaft of the first motor; the second synchronous pulley is arranged side by side with the first synchronous pulley and is connected by the first synchronous belt. The first bearing is mounted on the first bearing bracket and is located above the second synchronous pulley; the second bearing is mounted on the first bearing bracket and is located below the second synchronous pulley. The first rotating shaft is mounted on the first bearing bracket via the first bearing and the second bearing. The upper end of the first rotating shaft passes through the shaft center of the first bearing and the shaft hole of the sample disk in sequence and is fixedly connected to the sample disk. The lower end of the first rotating shaft passes through the shaft center of the second synchronous wheel and the shaft center of the second bearing in sequence and is driven to rotate by the second synchronous wheel. The output shaft of the first motor rotates, driving the first synchronous pulley to rotate. The first synchronous belt drives the second synchronous pulley to rotate synchronously, and the second synchronous pulley drives the first rotating shaft to rotate, thereby driving the sample disk and the reaction disk to rotate.

4. The sample preparation mechanism for pesticide residue detection according to claim 1, characterized in that, The second drive assembly also includes a second motor, a third synchronous pulley, a fourth synchronous pulley, a second synchronous belt, a second bearing bracket, a third bearing, a fourth bearing, and a bushing; The outer wall of the second rotating shaft is provided with a vertical sliding groove along its axial direction, and the height of the vertical sliding groove is the same as the lifting height of the liquid injection needle; The second motor is installed below the reagent tray; the third synchronous pulley is sleeved on the output shaft of the second motor; the fourth synchronous pulley is arranged side by side with the third synchronous pulley and is connected by the second synchronous belt; the fourth synchronous pulley is provided with a fastening groove along its radial direction, and the fastening groove is provided with steel balls and elastic fasteners; The third bearing is mounted on the second bearing bracket and is located above the fourth synchronous pulley; the fourth bearing is mounted on the second bearing bracket and is located below the fourth synchronous pulley. The second rotating shaft is provided with the bushing. The second rotating shaft and the bushing are mounted on the second bearing bracket through the third bearing and the fourth bearing. The upper end of the second rotating shaft and the bushing pass through the shaft center of the third bearing and the shaft hole of the reagent tray in sequence and extend upward. The lower end of the second rotating shaft passes through the shaft center of the fourth synchronous wheel and the shaft center of the fourth bearing in sequence and is driven to rotate by the fourth synchronous wheel. The bushing is provided with a radial through hole; the elastic fastener passes through the radial through hole and pushes the steel ball, pressing the steel ball against the vertical slide groove of the second rotating shaft; The output shaft of the second motor rotates, driving the third synchronous pulley to rotate, which in turn drives the fourth synchronous pulley to rotate synchronously via the second synchronous belt. The fourth synchronous pulley drives the second rotating shaft, the bushing, the second rotating shaft, and the liquid injection needle to rotate.

5. The sample preparation mechanism for pesticide residue detection according to claim 4, characterized in that, The second drive assembly further includes a lifting unit, which includes a connecting block, a lifting motor, a slider, and a guide rail; Therefore, the connecting block has a first connecting hole and a second connecting hole arranged side by side; The lifting motor is installed above the connecting block, and the lead screw of the lifting motor extends downward and passes through the second connecting hole, and is fixedly installed with the connecting block; The guide rail is installed parallel to the lead screw of the lifting motor; one side of the slider is slidably installed in cooperation with the guide rail, and the other side is fixedly installed with the connecting block; The lower end of the second rotating shaft passes through the first connecting hole and is fixedly installed with the connecting block through the first connecting hole; When the lead screw of the lifting motor moves up and down, it drives the slider to slide on the guide rail, and drives the connecting block, the second rotating shaft and the liquid injection needle to rise and fall. The vertical slide groove of the second rotating shaft and the steel ball generate relative movement.

6. The sample preparation mechanism for pesticide residue detection according to claim 1, characterized in that, The reagent tray is also provided with a needle washing position, which is adjacent to the reagent position and is used to install a cleaning device.

7. The sample preparation mechanism for pesticide residue detection according to claim 1, characterized in that, The delivery device includes a first valve, a buffer zone, a second valve, and a plunger pump connected in sequence; the first valve is connected to the injection needle via a pipeline; During liquid sample extraction, the plunger pump operates to provide suction, and the liquid sample is drawn into the buffer zone through the second valve, the first valve, and the liquid injection needle; During liquid sample injection, the plunger pump operates to provide thrust, releasing the liquid sample stored in the buffer zone through the second valve, the first valve, and the injection needle.

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