A method for detecting the antigen content of a biphase oil adjuvant vaccine

CN119716068BActive Publication Date: 2026-08-28NOVO BIOTECH CORP
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Patent Information

Application Number
CN202311210733.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-09-19
Publication Date
2026-08-28
Estimated Expiration
2043-09-19

AI Technical Summary

Technical Problem

第一种方法具有准确度高，灵敏性好的优点，但是该方法对疫苗抗原蛋白的结构要求非常高，蛋白的结构在高效液相色谱上检测应形成单一、特异的吸收峰，如果抗原蛋白结构多样形成多吸收峰，则不能采用该方法进行准确定量，且由于分析柱相对昂贵，不宜用于规模化的动物疫苗检测

Benefits of technology

[0022]与现有技术相比，本发明的检测方法具有如下有益效果：1)该方法对疫苗抗原蛋白的结构要求相对低，不需要单一的结构，对于某些多构象的蛋白也适用。2)该方法先对疫苗进行破乳，再进行冷丙酮沉淀，有效避免了油佐剂对抗原沉淀效率的干扰，也排除了油佐剂对后续抗原检测的干扰。3)该方法冷丙酮沉淀后，用SDS或PBS进行复溶，增加了复溶的效率，为后续抗原检测提高准确性和稳定性。

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Abstract

The application provides a detection method for antigen content of a biphasic oil emulsion vaccine, and the detection method comprises the following steps: 1) demulsifying the biphasic oil emulsion vaccine to obtain a demulsified liquid, 2) layering the demulsified liquid to obtain an antigen-containing water phase and filtering to obtain a water phase filtrate, 3) precipitating the water phase filtrate with cold acetone to obtain antigen, and redissolving the antigen precipitate to obtain a redissolution, and 4) detecting the total protein concentration of the redissolution by using a protein quantitative detection kit. The detection method has relatively low requirements for the structure of vaccine antigen proteins, does not need a single structure, is suitable for some proteins with multiple conformations, is suitable for a wide range, and is stable and accurate.
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Citation Information

Patent Citations

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  • Emulsion breaking method for aftosa oil emulsion inactivated vaccine

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  • Detection method for protein content and purity of oil emulsion protein subunit vaccine, and applications thereof

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