一株拟无枝酸菌YINM00005及其在制备安莎霉素类化合物中的应用
By optimizing culture conditions and purification techniques, a novel anesamcinolone compound with high yield and high purity was prepared using the pseudomycolic acid bacterium YINM00005, solving the problem of low yield in existing technologies and realizing the efficient preparation and large-scale production of anesamcinolone compounds.
CN119736186BActive Publication Date: 2026-07-17YUNNAN UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- YUNNAN UNIV
- Filing Date
- 2024-11-27
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
In the existing technology, strains capable of metabolizing and producing anesamcinolone compounds have low yields, which limits their application in drug development.
Method used
Four novel ansamycin-like compounds were prepared by fermentation with *Amylopectinobacterium yanhusuo* YINM00005, and by optimizing the culture medium and fermentation conditions, combined with silica gel column chromatography and high performance liquid chromatography purification.
Benefits of technology
It improves the yield and purity of anisomycin compounds, enriches the variety of anisomycins, provides an efficient preparation method, is suitable for large-scale production, and has mild reaction conditions and is environmentally friendly.
✦ Generated by Eureka AI based on patent content.
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Figure CN119736186B_ABST
Abstract
本发明公开了一株拟无枝酸菌YINM00005及其在制备安莎霉素类化合物中的应用，属于微生物技术领域。所述拟无枝酸菌YINM00005为云南昆明石蝉草内生菌，发酵后可大量代谢新颖的安莎霉素类化合物；发酵步骤主要包括：在适合的培养条件下发酵该菌株得到发酵液，经过溶剂提取浓缩后，结合柱层析法，可制备得到4种安莎霉素类化合物，发酵液中4种安莎霉素类化合物总浓度可达4.5‑8.6mg / L，提取分离后纯度可达98%以上，4种化合物均具有很强的抗肿瘤细胞毒活性。该方法成本较低，过程简单，环境友好，可用于该类安莎霉素类化合物的大量生产制备，不仅满足了低碳经济的需求，而且为后期安莎霉素类化合物的生产和相关产品开发奠定了基础。
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