Application of ntcrlk47 gene in relieving tobacco continuous cropping obstacles

CN119736330BActive Publication Date: 2026-07-21YUXI ZHONGYAN SEED CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YUXI ZHONGYAN SEED CO LTD
Filing Date
2024-12-30
Publication Date
2026-07-21

AI Technical Summary

Technical Problem

Long-term continuous tobacco cropping leads to the deterioration of soil physical and chemical properties, reduced enzyme activity, and accumulation of autotoxic substances, resulting in tobacco continuous cropping obstacles that are difficult to alleviate effectively with existing technologies.

Method used

By knocking out the NtCrRLK47 gene in tobacco, a tobacco mutant was constructed. The mutant was then planted in soil using Agrobacterium-mediated genetic transformation technology to interfere with gene expression and alleviate continuous cropping obstacles.

Benefits of technology

It significantly increases the fresh weight, stem length, and leaf area of ​​tobacco in continuous cropping, effectively alleviating continuous cropping obstacles and increasing tobacco yield.

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Abstract

The application discloses application of NtCrRLK47 gene in relieving tobacco continuous cropping obstacles, and the gene is numbered as Ntab0776290.1 in a Chinese tobacco genome database. The application also provides a method for relieving tobacco continuous cropping obstacles, and the method is achieved by knocking out or interfering with expression of the gene in tobacco, or constructing a tobacco mutant strain of tobacco NtCrRLK47 gene knockout. After the mutant material is planted, tobacco continuous cropping is carried out in soil, so that the continuous cropping obstacles of tobacco can be effectively relieved, and the yield of tobacco is improved. The method has application prospects in the field of tobacco planting.
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