A multi-legged DNA walker based on framework DNA functional materials, and a detection method and application thereof
CN119736369BActive Publication Date: 2026-08-28GUANGDONG OCEAN UNIVERSITY
Patent Information
- Application Number
- CN202410940825.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-15
- Publication Date
- 2026-08-28
- Estimated Expiration
- 2044-07-15
AI Technical Summary
Technical Problem
但目前鲜有将3D DNA步行器用于VP的检测，为了克服现有VP检测方法特异性较差，存在假阳性或假阴性现象的问题，有必要提供更多的有效方法和手段，实现VP快速、灵敏、特异性强、无酶检测，为VP的微量或痕量分析提供新方法
Benefits of technology
本发明基于DNA纳米技术，设计并制备了多种DNA功能材料，利用适配体对VP靶标的特异性识别，通过多足DNA步行器的信号触发和迁移扩增以及G四链体的组装信号输出，构建了一种能快速灵敏、高特异性检测VP的多足DNA步行器，实现对目标VP的检测。当目标VP不存在时，无法触发多足DNA步行器的迁移，燃料GF仍保持稳定的发夹结构，无检测信号产生。当目标VP存在时，多足DNA步行器触发迁移，GF发夹打开，G四链体与NMM特异性结合，实现荧光信号的增强。同时，提供一种基于多足DNA步行器对VP进行荧光传感检测的方法，VP浓度在102~107CFU/mL范围内，都具有良好的检测性能，LOD为23 CFU/mL；并通过VP的加标回收实验，证明该方法具有实际应用价值，为低浓度的VP的检测提供了一种新的、灵敏性高、特性性强、无酶、快捷且准确的检测方法。
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Figure CN119736369B_ABST
Abstract
The application discloses a multi-legged DNA walker based on a frame DNA functional material and a detection method and application thereof. The application provides a multi-legged DNA walker constructed based on a frame DNA functional material, which not only improves the detection sensitivity of a VP sensing method, but also improves the kinetic efficiency of the DNA walker through a specific DNA tetrahedron structure, and realizes fluorescence signal output in combination with a G-quadruplex / NMM system, so that the biological signal of VP is converted into a detectable fluorescence signal. Meanwhile, a fluorescence sensing and detection method based on the multi-legged DNA walker is established, and rapid, sensitive, specific and enzyme-entropy-driven detection of VP is realized, and a good linear relationship is presented in a range of 10 2 ~10 7 CFU / mL, the detection limit is as low as 23 CFU / mL, the sensitivity and specificity are good, and the method can be used for detection in different sample environments, and more new products and methods are provided for trace detection and analysis of VP.
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