一种与小麦抗赤霉病基因Qfhb.jaas-6B连锁的分子标记、引物组及其应用

By developing molecular markers and primer sets closely linked to the wheat scab resistance gene Qfhb.jaas-6B, rapid identification and breeding of scab-resistant wheat were achieved, solving the problems of single resistance genes and long breeding cycles in existing technologies, and improving breeding efficiency and disease resistance.

CN119736431BActive Publication Date: 2026-07-17JIANGSU ACAD OF AGRI SCI

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGSU ACAD OF AGRI SCI
Filing Date
2025-02-21
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Current technologies for breeding wheat resistance to Fusarium head blight rely on a very small number of resistance genes, which are insufficient to effectively reduce the damage in years when Fusarium head blight is prevalent. There is a lack of stable resistance genes and efficient breeding methods.

Method used

We developed molecular markers and primer sets closely linked to the wheat Fusarium head blight resistance gene Qfhb.jaas-6B. We then used quantitative real-time PCR amplification and fluorescence signal scanning to identify whether wheat DNA samples carry the Fusarium head blight resistance gene, which can be used to assist in selective breeding.

Benefits of technology

It provides a stable set of molecular markers and primers, enabling rapid identification and breeding of wheat resistant to Fusarium head blight, shortening the breeding cycle, improving the resistance effect of disease-resistant varieties, and laying the foundation for cloning new genes.

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Abstract

本发明属于小麦抗病育种技术领域,具体涉及一种与小麦抗赤霉病基因Qfhb.jaas‑6B连锁的分子标记、引物组及其应用;所述分子标记位于小麦6B染色体长臂上,当小麦为感赤霉病小麦时,所述小麦6B染色体第702386391个核苷酸为T,所述分子标记的核苷酸序列如SEQ ID No.1所示;当小麦为抗赤霉病小麦时,所述6B染色体第702386391个核苷酸为C,所述分子标记的核苷酸序列如SEQ ID No.2所示;所述引物组由核苷酸序列如SEQ ID No.5所示的前引物1、核苷酸序列如SEQ ID No.6所示的前引物2和核苷酸序列如SEQ ID No.7所示的后引物组成。
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