An ocular repair composition and uses thereof

Abstract

This invention relates to an eye repair composition and its application, wherein the eye repair composition comprises ecdysterol, fermented product of *Ganoderma lucidum*, and sodium heparin. This invention creatively discovers that the composition of ecdysterol, fermented product of *Ganoderma lucidum*, and sodium heparin has significant effects on the skin, especially the skin around the eyes, in improving skin brightness, reducing melanin content around the eyes, and eliminating dark circles. Furthermore, the three components exhibit a certain synergistic effect in the above-mentioned effects, making it very suitable for use in eye care products. It is gentle, non-irritating, and has good stability.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to an eye repair composition and its application. Background Technology

[0002] With the fast pace of modern life and increased work pressure, staying up late has become the norm, leading to a growing number of people experiencing dark circles under their eyes. Consequently, consumer demand for products that can effectively improve dark circles is increasing. The causes of dark circles are varied. Prolonged exposure to ultraviolet rays and post-inflammatory hyperpigmentation easily form pigmented dark circles, while insufficient sleep affects metabolism, causing dilation of blood vessels around the eyes and leading to vascular dark circles. Different causes require dark circle products to have more targeted effects to meet the needs of different users.

[0003] CN111759762A discloses a composition and essence for removing dark circles under the eyes, and their preparation methods. The composition includes elderberry extract, North African cedar bark extract, hydrolyzed collagen, methylsilanol mannitol ester, and sorbitol. This composition can effectively improve blood supply to blood vessels, enhance skin cell vitality, and reduce pigment deposition, thereby effectively removing dark circles, preventing their formation, and maintaining skin elasticity and firmness. The essence, based on the composition, can effectively remove dark circles and reduce cell texture, making the skin firmer and more elastic.

[0004] CN114652631A discloses a composition and cosmetic for removing dark circles under the eyes, and a method for preparing the same. The composition includes glucosyl hesperidin, carboxymethyl deacetylated chitosan, glycyrrhizin, palmitoyl tripeptide-5, and passion fruit extract. The components interact and coordinate to exert the best repair effect on the skin around the eyes, reducing the area of ​​severe pigmentation while improving the strength of capillaries and lymphatic vessels, reducing capillary permeability and blood stasis, and ultimately achieving the effect of reducing dark circles.

[0005] CN111728926A discloses an effective composition for removing dark circles under the eyes, its application, and a cosmetic preparation thereof. The composition uses Eriocaulon hydrochloride methanol extract, Magnolia officinalis extract, and 80% ethanol extract of Alternaria ostreatus as the main active ingredients. The combination of these three ingredients can significantly improve the microcirculation of the skin around the eyes, reduce pigmentation, protect the capillaries around the eyes, and repair the skin around the eyes. It has an ideal effect on pigmented dark circles and reduces the occurrence of complications from laser treatment of dark circles.

[0006] However, although the aforementioned cosmetics contain a large number of active ingredients, these active ingredients are difficult to penetrate deep into the skin to exert their effects. Therefore, it is of great significance to develop an eye repair composition that can penetrate deep into the skin to achieve the effect of brightening and lightening dark spots. Summary of the Invention

[0007] To address the shortcomings of existing technologies, the present invention aims to provide an eye repair composition and its application, specifically an eye repair composition that improves skin brightness, reduces melanin content around the eyes, and eliminates dark circles, and its application in the preparation of eye skincare products.

[0008] To achieve this objective, the present invention employs the following technical solution:

[0009] In a first aspect, the present invention provides an eye repair composition comprising ecdysterol, fermented product of Ganoderma lucidum, and sodium heparin.

[0010] Ecdysterol is a plant-derived, all-natural organic compound that can increase collagen synthesis, strengthen stress-damaged functional systems, and protect against damage caused by various environmental factors and stresses. Tibetan kefir mushroom is a complex symbiotic system composed of microorganisms, mainly lactobacilli, lactococci, and yeast. Fermented in a culture medium containing milk and Scutellaria baicalensis root powder, the resulting fermentation products can scavenge excess free radicals and inhibit the activity of related enzymes, achieving anti-inflammatory, anti-aging, and repairing effects. Sodium heparin can increase skin vascular permeability, improve local vascular circulation, and thus increase skin metabolism.

[0011] This invention creatively discovers that a composition consisting of ecdysterol, fermented Ganoderma lucidum, and sodium heparin has significant effects on the skin, especially the skin around the eyes, in improving skin brightness, reducing melanin content around the eyes, and eliminating dark circles. Moreover, the three components have a certain synergistic effect on the above effects, making it very suitable for use in eye care products. It is gentle, non-irritating, and has good stability.

[0012] Preferably, the fermented product of *Ganoderma lucidum* is prepared by a method comprising the following steps:

[0013] (1) The activated Tibetan Ganoderma lucidum spawn was inoculated into a seed culture medium and cultured to obtain seed liquid;

[0014] (2) The seed liquid was inoculated into a fermentation medium and cultured. The fermentation product was filtered and the filtrate was dried to obtain the fermentation product of the Tibetan Ganoderma lucidum.

[0015] The fermentation medium consists of milk and Scutellaria baicalensis root powder.

[0016] Preferably, in step (1), the inoculation amount of the activated Tibetan Ganoderma lucidum strain is 1-15%, for example, it can be 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, etc.

[0017] Preferably, in step (1), the culture temperature is 25-30℃, for example, it can be 25℃, 25.5℃, 26℃, 26.5℃, 27℃, 27.5℃, 28℃, 28.5℃, 29℃, 29.5℃, 30℃, etc.; the culture time is 18-30h, for example, it can be 18h, 19h, 20h, 21h, 22h, 23h, 24h, 25h, 26h, 27h, 28h, 29h, 30h, etc.

[0018] Preferably, in step (2), the inoculation amount of the seed liquid is 5-10%, for example, it can be 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, etc.

[0019] Preferably, in step (2), the culture temperature is 25-30℃, for example, it can be 25℃, 25.5℃, 26℃, 26.5℃, 27℃, 27.5℃, 28℃, 28.5℃, 29℃, 29.5℃, 30℃, etc.; the culture time is 18-30h, for example, it can be 18h, 19h, 20h, 21h, 22h, 23h, 24h, 25h, 26h, 27h, 28h, 29h, 30h, etc.

[0020] Preferably, the Scutellaria baicalensis root powder has a mass percentage content of 1-2% in milk, for example, it can be 1%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2%, etc.

[0021] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0022] Preferably, the eye repair composition comprises, by weight, 0.1-5 parts ecdysterol, 1-8 parts fermented product of Ganoderma lucidum, and 0.01-5 parts sodium heparin.

[0023] Furthermore, when the above three components are combined in a specific mass ratio, they have a more significant effect on improving skin brightness, reducing melanin content around the eyes, and eliminating dark circles.

[0024] The weight parts of the ecdysterol can be 0.1 parts, 0.3 parts, 0.5 parts, 0.7 parts, 1 part, 1.2 parts, 1.5 parts, 1.7 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

[0025] The weight percentages of the fermented product of Tibetan Ganoderma lucidum can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, etc.

[0026] The weight parts of the heparin sodium can be 0.01 parts, 0.03 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.2 parts, 0.5 parts, 0.8 parts, 1 part, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

[0027] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0028] More preferably, the eye repair composition comprises, by weight, 0.5-2 parts ecdysterol, 2-5 parts fermented product of Ganoderma lucidum, and 0.05-2 parts sodium heparin.

[0029] Preferably, the eye repair composition further includes a solvent.

[0030] Preferably, the solvent comprises a polyol and deionized water.

[0031] Preferably, the polyol includes any one or a combination of at least two of ethylene glycol, propylene glycol, butylene glycol, pentanediol, dipropylene glycol, glycerol, polyethylene glycol, or sorbitol.

[0032] In a second aspect, the present invention provides a method for preparing the eye repair composition as described in the first aspect, the method comprising: mixing and homogenizing ecdysterol, fermented product of Ganoderma lucidum and sodium heparin to obtain the eye repair composition.

[0033] Preferably, the ecdysterol is mixed with a solvent, heated and kept warm, cooled and then mixed with fermented Ganoderma lucidum and sodium heparin, and homogenized to obtain the eye repair composition.

[0034] Preferably, the heating refers to raising the temperature to 85-90℃, for example, 85℃, 85.5℃, 86℃, 86.5℃, 87℃, 87.5℃, 88℃, 88.5℃, 89℃, 89.5℃, 90℃, etc.; the holding time is 20-30 minutes, for example, 20 minutes, 21 minutes, 22 minutes, 23 minutes, 24 minutes, 25 minutes, 26 minutes, 27 minutes, 28 minutes, 29 minutes, 30 minutes, etc.; the cooling temperature is 30-35℃, for example, 30℃, 30.5℃, 31℃, 31.5℃, 32℃, 32.5℃, 33℃, 33.5℃, 34℃, 34.5℃, 35℃, etc.

[0035] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0036] Thirdly, the present invention provides the use of the eye repair composition as described in the first aspect in the preparation of cosmetics.

[0037] Preferably, the cosmetic includes eye cream, eye gel, eye lotion, eye serum, eye mask, eye patch, or eye gel.

[0038] Preferably, the eye repair composition has a mass percentage content of 5-35% in the cosmetic, for example, it can be 5%, 8%, 10%, 12%, 15%, 20%, 23%, 25%, 28%, 30%, 35%, etc. Other specific values ​​within this range can be selected, and will not be elaborated here.

[0039] Preferably, the cosmetic also includes any one or a combination of at least two of the following: solvent, moisturizer, thickener, solubilizer, pH adjuster, chelating agent, preservative, skin conditioner, or fragrance.

[0040] Fourthly, the present invention provides a multi-effect repair eye cream, wherein the multi-effect repair eye cream comprises, by weight, 5-35 parts of the eye repair composition described in the first aspect, 3.5-8 parts of emulsifier, 2.5-9 parts of emollient, 1.3-4 parts of antioxidant, 13-17 parts of moisturizer, 0.5-0.8 parts of thickener, 0.3-0.6 parts of pH adjuster, and deionized water.

[0041] The weight parts of the eye repair composition can be 5 parts, 8 parts, 10 parts, 12 parts, 15 parts, 20 parts, 23 parts, 25 parts, 28 parts, 30 parts, 35 parts, etc.

[0042] The emulsifier can be present in parts by weight of 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, etc.

[0043] The emollient can be present in parts by weight of 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, or 9 parts.

[0044] The antioxidant can be present in parts by weight of 1.3, 1.5, 2, 2.5, 3, 3.5, or 4 parts, etc.

[0045] The weight parts of the humectant can be 13 parts, 13.5 parts, 14 parts, 14.5 parts, 15 parts, 15.5 parts, 16 parts, 16.5 parts, 17 parts, etc.

[0046] The thickener can be present in parts by weight of 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, etc.

[0047] The pH adjuster can be present in weight parts of 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, etc.

[0048] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0049] Preferably, the emulsifier comprises any one or a combination of at least two of C14-22 alkyl alcohols / C12-20 alkyl glucosides, hydrogenated lecithin, behenol, sorbitan stearate, sorbitan isostearate, polyglycerol-10 myristate, or polysorbate-60.

[0050] Preferably, the emollient comprises any one or a combination of at least two of the following: C10-18 fatty acid triglycerides, polydimethylsiloxane, squalane, cetyl ethylhexanoate, diisostearyl malate, jojoba seed oil, or rice germ oil.

[0051] Preferably, the emollient comprises a combination of C10-18 fatty acid triglycerides, polydimethylsiloxane, and squalane.

[0052] In this invention, the preferred emollient is a combination of C10-18 fatty acid triglycerides, polydimethylsiloxane, and squalane. These three ingredients have a synergistic effect, which can further enhance the brightening and lightening effect of the eye cream involved in this invention.

[0053] Preferably, the emollient comprises, by weight, 0.6-3 parts of C10-18 fatty acid triglycerides, 0.5-4 parts of polydimethylsiloxane, and 0.5-6 parts of squalane.

[0054] In this invention, when the above three ingredients are combined in a specific mass ratio, the brightening and darkening effects of the eye cream involved in this invention are significantly enhanced.

[0055] The weight parts of the C10-18 fatty acid triglycerides can be 0.6 parts, 0.8 parts, 1 part, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.3 parts, 2.5 parts, 2.8 parts, 3 parts, etc.

[0056] The polydimethylsiloxane can be in the following weight proportions: 0.5 parts, 0.8 parts, 1 part, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.3 parts, 2.5 parts, 2.8 parts, 3 parts, 3.5 parts, 4 parts, etc.

[0057] The weight parts of squalane can be 0.5 parts, 0.8 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, etc.

[0058] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0059] Preferably, the antioxidant comprises any one or a combination of at least two of meadowfoam seed oil, tocopheryl acetate, retinyl palmitate, or p-hydroxyacetophenone.

[0060] Preferably, the antioxidant comprises a combination of meadowfoam seed oil and tocopheryl acetate.

[0061] Meadowfoam seed oil contains over 98% long-chain fatty acids with antioxidant properties. It forms a water-locking film on the skin's surface, preventing dryness and providing powerful antioxidant effects. It also smooths skin texture, deeply repairs skin damage, and improves overall skin contour. Tocopheryl acetate softens skin, retains moisture, promotes wound healing, prevents inflammation, prevents rough and cracked skin, and improves fine lines and dark spots. It also has strong reducing properties, eliminating free radicals and reducing UV damage to the skin.

[0062] In this invention, the antioxidant is preferably a combination of meadowfoam seed oil and tocopheryl acetate. The two components have a synergistic effect, which can further enhance the brightening and lightening effect of the eye cream involved in this invention.

[0063] Preferably, the antioxidant comprises, by weight, 1-3 parts meadowfoam seed oil and 0.3-1 parts tocopheryl acetate.

[0064] In this invention, when the two components are combined in a specific mass ratio, the brightening and darkening effects of the eye cream involved in this invention are significantly enhanced.

[0065] The weight parts of the meadowfoam seed oil can be 1 part, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.3 parts, 2.5 parts, 2.8 parts, 3 parts, etc.

[0066] The weight parts of the tocopherol acetate can be 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 part, etc.

[0067] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0068] Preferably, the moisturizer includes any one or a combination of at least two of glycerin, 1,3-butanediol, propylene glycol, 1,2-pentanediol, or sodium hyaluronate.

[0069] Preferably, the thickener comprises carbomer and / or xanthan gum.

[0070] Preferably, the pH adjuster comprises aminomethylpropanol.

[0071] Preferably, the multi-effect repair eye cream further includes 0.1-0.3 parts of preservative by weight.

[0072] The preservative can be present in parts by weight of 0.1, 0.12, 0.15, 0.18, 0.2, 0.23, 0.25, 0.28, 0.3, etc.

[0073] Preferably, the multi-effect repair eye cream further includes 0.01-0.03 parts of fragrance by weight.

[0074] The flavoring can be expressed in parts by weight of 0.01, 0.012, 0.015, 0.018, 0.02, 0.023, 0.025, 0.028, or 0.03.

[0075] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0076] Fifthly, the present invention provides a method for preparing a multi-effect repair eye cream as described in the fourth aspect, the method comprising:

[0077] (1) Add the emulsifier, emollient and antioxidant to the oil phase pot, stir and heat, keep warm to obtain phase A;

[0078] The humectant, thickener, pH adjuster and deionized water are added to an aqueous phase pot, stirred and heated, and kept warm to obtain phase B.

[0079] (2) Mix phase A and phase B in an emulsifying pan and homogenize them;

[0080] (3) Cool down, add the eye repair composition, homogenize, filter and discharge to obtain the multi-effect repair eye cream.

[0081] Preferably, the stirring speed in step (1) for preparing phase A is 800-1200 rpm, for example, 800 rpm, 850 rpm, 900 rpm, 950 rpm, 1000 rpm, 1050 rpm, 1100 rpm, 1150 rpm, 1200 rpm, etc.; the heating refers to raising the temperature to 80-90℃, for example, 80℃, 81℃, 82℃, 83℃, 84℃, 85℃, 86℃, 87℃, 88℃, 89℃, 90℃, etc.; the holding time is 5-15 min, for example, 5 min, 6 min, 7 min, 8 min, 9 min, 10 min, 11 min, 12 min, 13 min, 14 min, 15 min, etc.

[0082] Preferably, the stirring speed in step (1) for preparing phase B is 800-1200 rpm, for example, 800 rpm, 850 rpm, 900 rpm, 950 rpm, 1000 rpm, 1050 rpm, 1100 rpm, 1150 rpm, 1200 rpm, etc.; the heating refers to raising the temperature to 85-95℃, for example, 85℃, 86℃, 87℃, 88℃, 89℃, 90℃, 91℃, 92℃, 93℃, 94℃, 95℃, etc.; the holding time is 10-20 min, for example, 10 min, 11 min, 12 min, 13 min, 14 min, 15 min, 16 min, 17 min, 18 min, 19 min, 20 min, etc.

[0083] Preferably, the homogenization time in step (2) is 3-7 min, for example, it can be 3 min, 3.5 min, 4 min, 4.5 min, 5 min, 5.5 min, 6 min, 6.5 min, 7 min, etc.; the homogenization speed is 2500-3500 rpm, for example, it can be 2500 rpm, 2600 rpm, 2700 rpm, 2800 rpm, 2900 rpm, 3000 rpm, 3100 rpm, 3200 rpm, 3300 rpm, 3400 rpm, 3500 rpm, etc.

[0084] Preferably, the cooling in step (3) refers to cooling to 40-50℃, for example, 40℃, 41℃, 42℃, 43℃, 44℃, 45℃, 46℃, 47℃, 48℃, 49℃, 50℃, etc.; the homogenization time is 10-20min, for example, 10min, 11min, 12min, 13min, 14min, 15min, 16min, 17min, 18min, 19min, 20min, etc.

[0085] Other specific point values ​​within the range of the above values ​​can be selected, and will not be elaborated on here.

[0086] Compared with the prior art, the present invention has the following beneficial effects:

[0087] (1) The three components of the eye repair composition of the present invention, namely ecdysterol, fermented product of Ganoderma lucidum and sodium heparin, have a synergistic effect and have significant effects on improving skin brightness, reducing melanin content around the eyes and eliminating dark circles. It is very suitable for use in eye care products, is gentle and non-irritating, and has good stability.

[0088] (2) The eye repair composition is combined with other components to prepare a multi-effect repair eye cream that can increase skin nutrition, deeply moisturize the skin, and also has the effects of moisturizing, anti-oxidation, lightening dark circles, improving skin brightness and firming the skin.

[0089] (3) The three components, C10-18 fatty acid triglycerides, polydimethylsiloxane and squalane, have a synergistic effect. While softening the skin and moisturizing, they can further enhance the dark spot brightening effect of the multi-effect repair eye cream of this invention. When the above three components are combined in a specific mass ratio, the effect of enhancing dark spot brightening is more significant.

[0090] (4) The two ingredients, meadowfoam seed oil and tocopheryl acetate, work synergistically to lock in moisture, moisturize, resist inflammation and resist oxidation, further enhancing the dark spot brightening effect of the multi-effect repair eye cream of this invention; when the above two ingredients are combined in a specific mass ratio, the effect of enhancing dark spot brightening is more significant. Detailed Implementation

[0091] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0092] The sources of the active ingredients in the products involved in the following preparation examples, embodiments, comparative examples, application examples, and comparative application examples are as follows (only the active ingredients are shown; necessary excipients contained in other commercially available raw materials are not described):

[0093]

[0094]

[0095] All other raw materials can be used as long as they are purchased from authorized distributors.

[0096] Preparation Example 1

[0097] This preparation example provides a fermented product of *Ganoderma lucidum*, which is prepared by a method including the following steps:

[0098] (1) Preparation of Scutellaria baicalensis root powder: After drying, Scutellaria baicalensis root is pulverized and sieved to obtain Scutellaria baicalensis root powder, which is then packaged, sterilized and put into use.

[0099] (2) Preparation of fermentation culture medium: Add 1.5% by weight of Scutellaria baicalensis root powder to milk and mix well;

[0100] (3) The activated Tibetan Ganoderma lucidum spawn was inoculated into the seed culture medium at an inoculation rate of 7.5% and cultured at 25°C for 24 hours to obtain the seed liquid;

[0101] (4) The seed liquid was inoculated into the fermentation medium at an inoculation rate of 7.5%, and cultured at 25°C for 24 hours. The fermentation product was filtered, and the filtrate was dried to obtain the fermentation product of the Tibetan Ganoderma lucidum.

[0102] Preparation Example 2

[0103] This preparation example provides a fermented product of *Ganoderma lucidum*, which is prepared by a method including the following steps:

[0104] (1) Preparation of Scutellaria baicalensis root powder: After drying, Scutellaria baicalensis root is pulverized and sieved to obtain Scutellaria baicalensis root powder, which is then packaged, sterilized and put into use.

[0105] (2) Preparation of fermentation culture medium: Add 1% by weight of Scutellaria baicalensis root powder to milk and mix well;

[0106] (3) The activated Tibetan Ganoderma lucidum spawn was inoculated into the seed culture medium at an inoculation rate of 5% and cultured at 25°C for 26 hours to obtain the seed liquid;

[0107] (4) The seed liquid was inoculated into the fermentation medium at an inoculation rate of 10%, and cultured at 25°C for 22 hours. The fermentation product was filtered, and the filtrate was dried to obtain the fermentation product of the Tibetan Ganoderma lucidum.

[0108] Preparation Example 3

[0109] This preparation example provides a fermented product of *Ganoderma lucidum*, which is prepared by a method including the following steps:

[0110] (1) Preparation of Scutellaria baicalensis root powder: After drying, Scutellaria baicalensis root is pulverized and sieved to obtain Scutellaria baicalensis root powder, which is then packaged, sterilized and put into use.

[0111] (2) Preparation of fermentation culture medium: Add 2% by weight of Scutellaria baicalensis root powder to milk and mix well;

[0112] (3) The activated Tibetan Ganoderma lucidum spawn was inoculated into the seed culture medium at an inoculation rate of 10% and cultured at 25°C for 22 hours to obtain the seed liquid;

[0113] (4) The seed liquid was inoculated into the fermentation medium at an inoculation rate of 5%, and cultured at 25°C for 26 hours. The fermentation product was filtered, and the filtrate was dried to obtain the fermentation product of the Tibetan Ganoderma lucidum.

[0114] Example 1

[0115] This embodiment provides an eye repair composition comprising, by weight, the following active ingredients: 1.5 parts of ecdysterol, 3 parts of fermented Ganoderma lucidum obtained in Preparation Example 1, and 1 part of sodium heparin.

[0116] The preparation method is as follows: 1.5 parts of ecdysterol, 7 parts of ethylene glycol, and 87.5 parts of deionized water are mixed, heated to 85°C, kept at the temperature for 20 minutes, and then cooled to 30°C. The mixture is then mixed with 3 parts of the fermented product of *Ganoderma lucidum* obtained in Preparation Example 1 and 1 part of sodium heparin, homogenized, and the mass percentage of the eye repair composition in the final product is 50%.

[0117] Example 2

[0118] This embodiment provides an eye repair composition comprising, by weight, the following active ingredients: 0.5 parts of ecdysterol, 2 parts of fermented Ganoderma lucidum obtained in Preparation Example 2, and 0.05 parts of sodium heparin.

[0119] The preparation method is as follows: 0.5 parts of ecdysterol, 4 parts of propylene glycol, and 93.45 parts of deionized water are mixed, heated to 87°C, kept at this temperature for 25 minutes, and then cooled to 32°C. The mixture is then mixed with 2 parts of the fermented product of *Ganoderma lucidum* obtained in Preparation Example 2 and 0.05 parts of sodium heparin, homogenized, and the mass percentage of the eye repair composition in the final product is 50%.

[0120] Example 3

[0121] This embodiment provides an eye repair composition comprising, by weight, the following active ingredients: 2 parts ecdysterol, 5 parts fermented product of Ganoderma lucidum obtained in Preparation Example 3, and 2 parts sodium heparin.

[0122] The preparation method is as follows: 2 parts of ecdysterol, 10 parts of butylene glycol, and 81 parts of deionized water are mixed, heated to 90°C, kept at 90°C for 30 minutes, and then cooled to 35°C. The mixture is then mixed with 5 parts of the fermented product of *Ganoderma lucidum* obtained in Preparation Example 3 and 2 parts of sodium heparin, homogenized, and the mass percentage of the eye repair composition in the final product is 50%.

[0123] Example 4

[0124] This embodiment provides an eye repair composition comprising, by weight, the following active ingredients: 1 part ecdysterol, 4 parts fermented product of Ganoderma lucidum obtained in Preparation Example 1, and 0.08 parts sodium heparin.

[0125] The preparation method is as follows: 1 part ecdysterol is mixed with 5 parts pentylene glycol and 89.92 parts deionized water, heated to 85°C, kept at the temperature for 20 min and then cooled to 30°C. It is then mixed with 4 parts of the fermented product of *Ganoderma lucidum* obtained in Preparation Example 1 and 0.08 parts of sodium heparin, homogenized, and the mass percentage of the eye repair composition in the final product is 50%.

[0126] Example 5

[0127] This embodiment provides an eye repair composition, which differs from Example 1 only in that it contains 6 parts of ecdysterol, 3 parts of fermented Ganoderma lucidum obtained in Preparation Example 1, and 1 part of sodium heparin.

[0128] The preparation method is the same as in Example 1.

[0129] Example 6

[0130] This embodiment provides an eye repair composition, which differs from Example 1 only in that it contains 1.5 parts of ecdysterol, 9 parts of fermented Ganoderma lucidum obtained in Preparation Example 1, and 1 part of sodium heparin.

[0131] The preparation method is the same as in Example 1.

[0132] Example 7

[0133] This embodiment provides an eye repair composition, which differs from Example 1 only in that it contains 1.5 parts of ecdysterol, 3 parts of the fermented product of Ganoderma lucidum obtained in Preparation Example 1, and 6 parts of sodium heparin.

[0134] The preparation method is the same as in Example 1.

[0135] Comparative Example 1

[0136] This comparative example provides an eye repair composition that differs from Example 1 only in that it does not contain ecdysterol, and its reduced amount is proportionally allocated to the other two ingredients.

[0137] The preparation method is the same as in Example 1.

[0138] Comparative Example 2

[0139] This comparative example provides an eye repair composition that differs from Example 1 only in that it does not contain the fermented product of *Ganoderma lucidum* obtained in Preparation Example 1, and the reduced amount of the product is proportionally allocated to the other two ingredients.

[0140] The preparation method is the same as in Example 1.

[0141] Comparative Example 3

[0142] This comparative example provides an eye care composition that differs from Example 1 only in that it does not contain sodium heparin, and its reduced amount is proportionally allocated to the other two ingredients.

[0143] The preparation method is the same as in Example 1.

[0144] Comparative Example 4

[0145] This comparative example provides an effective substance, which is a single component, ecdysterol.

[0146] Comparative Example 5

[0147] This comparative example provides an effective substance, which is the fermented product of *Ganoderma lucidum* obtained from Example 1, a single-component preparation.

[0148] Comparative Example 6

[0149] This comparative example provides an effective substance, which is a single component, sodium heparin.

[0150] Application Example 1

[0151] This application example provides a multi-effect repair eye cream, which comprises the following components by weight:

[0152]

[0153] Its preparation method is as follows:

[0154] (1) Add emulsifier, emollient and antioxidant to oil phase pot, stir at 1000 rpm, heat to 85℃, keep warm for 10 min to obtain phase A;

[0155] Add humectant, thickener, pH adjuster and deionized water to the aqueous phase pot, stir at 1000 rpm, heat to 90°C, keep warm for 15 min to obtain phase B;

[0156] (2) Mix phase A and phase B in an emulsifying pot and homogenize at 3000 rpm for 5 min;

[0157] (3) Cool down to 45°C, add the eye repair composition, homogenize for 15 minutes, filter and discharge to obtain the multi-effect repair eye cream.

[0158] Application Example 2

[0159] This application example provides a multi-effect repair eye cream, which differs from Application Example 1 only in that "20 parts of the eye repair composition prepared in Example 1" is replaced with "10 parts of the eye repair composition prepared in Example 2", while other raw materials and contents remain unchanged.

[0160] The preparation method is the same as in Application Example 1.

[0161] Application Example 3

[0162] This application example provides a multi-effect repair eye cream, which differs from Application Example 1 only in that "20 parts of the eye repair composition prepared in Example 1" is replaced with "15 parts of the eye repair composition prepared in Example 3", while other raw materials and contents remain unchanged.

[0163] The preparation method is the same as in Application Example 1.

[0164] Application Example 4

[0165] This application example provides a multi-effect repair eye cream, which differs from Application Example 1 only in that "20 parts of the eye repair composition prepared in Example 1" is replaced with "30 parts of the eye repair composition prepared in Example 4", while other raw materials and contents remain unchanged.

[0166] The preparation method is the same as in Application Example 1.

[0167] Application Example 5-7

[0168] This application example provides a multi-effect repair eye cream, which differs from Application Example 1 only in that "the eye repair composition prepared in Example 1" is replaced with "the eye repair composition prepared in Examples 5-7", while other raw materials and contents remain unchanged.

[0169] The preparation method is the same as in Application Example 1.

[0170] Application Example 8

[0171] This application example provides a multi-effect repair eye cream, which differs from Application Example 1 only in that it does not contain tocopheryl acetate, the reduction of which is made up by meadowfoam seed oil, while the other ingredients and contents remain unchanged.

[0172] The preparation method is the same as in Application Example 1.

[0173] Application Example 9

[0174] This application example provides a multi-effect repair eye cream, which differs from Application Example 1 only in that it does not contain meadowfoam seed oil, the reduction of which is made up by tocopheryl acetate, while the other ingredients and their contents remain unchanged.

[0175] The preparation method is the same as in Application Example 1.

[0176] Application Example 10

[0177] This application example provides a multi-effect repair eye cream, which differs from Application Example 1 only in that it does not contain C10-18 fatty acid triglycerides, and the reduced amount is proportionally allocated to the other two ingredients of the emollient, while the other raw materials and contents remain unchanged.

[0178] The preparation method is the same as in Application Example 1.

[0179] Application Example 11

[0180] This application example provides a multi-effect repair eye cream, which differs from Application Example 1 only in that it does not contain polydimethylsiloxane, and its reduced amount is proportionally allocated to the other two ingredients of the emollient, while the other raw materials and their contents remain unchanged.

[0181] The preparation method is the same as in Application Example 1.

[0182] Application Example 12

[0183] This application example provides a multi-effect repair eye cream, which differs from Application Example 1 only in that it does not contain squalane, and its reduced amount is proportionally allocated to the other two ingredients of the emollient, while the other raw materials and their contents remain unchanged.

[0184] The preparation method is the same as in Application Example 1.

[0185] Application Example 13

[0186] This application example provides a multi-effect repair eye cream, which differs from Application Example 1 only in that: 0.5 parts of C10-18 fatty acid triglycerides, 4.5 parts of polydimethylsiloxane, and 6.5 parts of squalane are added, while the other raw materials and their contents remain unchanged.

[0187] The preparation method is the same as in Application Example 1.

[0188] Comparative Application Examples 1-6

[0189] This comparative application example provides a multi-effect repair eye cream, which differs from Application Example 1 only in that the proportions of "eye repair composition prepared in Example 1" are replaced with "eye repair compositions prepared in Comparative Examples 1-6", while other raw materials and contents remain unchanged.

[0190] The preparation method is the same as in Application Example 1.

[0191] Comparative Application Example 7

[0192] This comparative application example provides a multi-effect repair eye cream, which differs from application example 1 only in that it does not contain the eye repair composition prepared in example 1, the reduction of which is made up by water, while the other raw materials and contents remain unchanged.

[0193] The preparation method is the same as in Application Example 1.

[0194] Test Example 1: Skin Safety Test

[0195] The multi-effect repair eye cream prepared in Application Examples 1-13 was placed in a patch applicator. The patch applicator containing the test substance was then applied to the flexor side of the forearm of 30 subjects. The patch applicator was gently pressed with the palm of the hand to ensure even application to the skin for 24 hours. Skin irritation and sensitization were observed according to Table 1 (Skin reaction grading standard for closed patch test) 30 min, 24 h, and 48 h after the patch applicator was removed, and the observation results were recorded.

[0196] Table 1

[0197]

[0198] The results showed that no adverse skin reactions occurred in the products of Application Examples 1-13, indicating that the eye repair composition and eye cream involved in the present invention are safe and non-irritating to the skin.

[0199] Test Example 2: Test on the effect of reducing dark circles

[0200] (1) Test samples: Multi-effect repair eye creams prepared using Examples 1-13 and Comparative Examples 1-7;

[0201] (2) Subject population: 35 healthy adult Chinese women aged 25-60 with problems such as dull skin tone, age spots, and dark circles around the eyes were selected;

[0202] (3) Test method: After cleansing and toning in the morning and evening, the subjects applied an appropriate amount of eye cream to the eye area, spread it evenly and massaged it until absorbed. Before the trial and after 8 weeks of trial, the melanin value, skin color L* value and skin color b* value of the eye area were tested with the VISIA skin analyzer, and photos were taken and visual assessments were performed to calculate ITA°.

[0203] (4) Evaluation criteria: A decrease in melanin value indicates that it has the effect of reducing melanin content around the eyes; an increase in skin color L* value indicates that it has the effect of improving skin brightness; an increase in ITA° indicates that it has the effect of improving dark circles.

[0204] The test results are shown in Table 2:

[0205] Table 2

[0206]

[0207] As shown in Table 2, the eye repair composition and eye cream involved in this invention have significant effects in improving skin brightness, reducing melanin content around the eyes, and eliminating dark circles. They are very suitable for use in eye care products, are gentle and non-irritating, and have good stability. Ecdysterol, fermented Ganoderma lucidum, and sodium heparin mutually enhance these effects, exhibiting an unexpected synergistic effect. Furthermore, the formulation of emollients and antioxidants also significantly impacts the product's brightening and lightening effects.

[0208] The applicant declares that the present invention is illustrated by the above embodiments to demonstrate an eye repair composition and its application, but the present invention is not limited to the above embodiments, that is, it does not mean that the present invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0209] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0210] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A multi-effect repairing eye cream, characterized in that, The multi-effect repair eye cream comprises, by weight, 5-35 parts of an eye repair composition, 3.5-8 parts of an emulsifier, 2.5-9 parts of a moisturizer, 1.3-4 parts of an antioxidant, 13-17 parts of a humectant, 0.5-0.8 parts of a thickener, 0.3-0.6 parts of a pH adjuster, and deionized water; The eye repair composition comprises, by weight, 0.1-5 parts ecdysterol, 1-8 parts fermented product of Ganoderma lucidum, and 0.01-5 parts sodium heparin; the eye repair composition also includes a solvent. The fermented product of *Ganoderma lucidum* is prepared by a method comprising the following steps: (1) inoculating activated *Ganoderma lucidum* spawn into a seed culture medium to obtain a seed liquid; (2) inoculating the seed liquid into a fermentation culture medium, filtering the fermentation product, and drying the filtrate to obtain the fermented product of *Ganoderma lucidum*; the inoculation amount of the seed liquid is 5-10%; the components of the fermentation culture medium include milk and *Scutellaria baicalensis* root powder; the mass percentage of *Scutellaria baicalensis* root powder in the milk is 1-2%; The emollient comprises, by weight, 0.6-3 parts of C10-18 fatty acid triglycerides, 0.5-4 parts of polydimethylsiloxane, and 0.5-6 parts of squalane; The antioxidants, by weight, comprise 1-3 parts meadowfoam seed oil and 0.3-1 parts tocopheryl acetate.

2. The multi-effect repair eye cream as described in claim 1, characterized in that, The eye repair composition comprises, by weight, 0.5-2 parts ecdysterol, 2-5 parts fermented product of Ganoderma lucidum, and 0.05-2 parts sodium heparin.

3. The multi-effect repair eye cream as described in claim 1, characterized in that, The solvent includes polyols and deionized water.

4. The multi-effect repair eye cream as described in claim 3, characterized in that, The polyols include any one or a combination of at least two of ethylene glycol, propylene glycol, butylene glycol, pentanediol, dipropylene glycol, glycerol, polyethylene glycol, or sorbitol.

5. The multi-effect repair eye cream as described in claim 1, characterized in that, In step (1), the inoculation amount of the activated Tibetan Ganoderma lucidum strain is 1-15%.

6. The multi-effect repair eye cream as described in claim 1, characterized in that, In step (1), the culture temperature is 25-30℃ and the culture time is 18-30 h.

7. The multi-effect repair eye cream as described in claim 1, characterized in that, In step (2), the culture temperature is 25-30℃ and the culture time is 18-30 h.

8. The multi-effect repair eye cream as described in claim 1, characterized in that, The preparation method of the eye repair composition includes: mixing and homogenizing ecdysterol, fermented product of Ganoderma lucidum, sodium heparin and solvent to obtain the eye repair composition.

9. The multi-effect repair eye cream as described in claim 8, characterized in that, The ecdysterol was mixed with a solvent, heated and kept warm, and then cooled and mixed with fermented Ganoderma lucidum and sodium heparin. The mixture was homogenized to obtain the eye repair composition.

10. The multi-effect repair eye cream as described in claim 9, characterized in that, The heating refers to raising the temperature to 85-90℃, the holding time is 20-30 minutes, and the cooling temperature is 30-35℃.

11. The multi-effect repair eye cream as described in claim 1, characterized in that, The emulsifier comprises any one or a combination of at least two of the following: C14-22 alkyl alcohol / C12-20 alkyl glucoside, hydrogenated lecithin, behenol, sorbitan stearate, sorbitan isostearate, polyglycerol-10 myristate, or polysorbate-60.

12. The multi-effect repair eye cream as described in claim 1, characterized in that, The emollient also includes any one or a combination of at least two of cetyl ethylhexanoate, diisostearyl malate, jojoba seed oil, or rice germ oil.

13. The multi-effect repair eye cream as described in claim 1, characterized in that, The antioxidants also include retinyl palmitate and / or p-hydroxyacetophenone.

14. The multi-effect repair eye cream as described in claim 1, characterized in that, The moisturizer includes any one or a combination of at least two of glycerin, 1,3-butanediol, propylene glycol, 1,2-pentanediol, or sodium hyaluronate.

15. The multi-effect repair eye cream as described in claim 1, characterized in that, The thickeners include carbomer and / or xanthan gum.

16. The multi-effect repair eye cream as described in claim 1, characterized in that, The pH adjuster includes aminomethylpropanol.

17. The multi-effect repair eye cream as described in claim 1, characterized in that, The multi-effect repair eye cream also includes 0.1-0.3 parts of preservative by weight.

18. The multi-effect repair eye cream as described in claim 1, characterized in that, The multi-effect repair eye cream also includes 0.01-0.03 parts fragrance by weight.

19. The method for preparing the multi-effect repair eye cream according to any one of claims 1-18, characterized in that, The preparation method includes: (1) Add the emulsifier, emollient and antioxidant to the oil phase pot, stir and heat, keep warm to obtain phase A; The humectant, thickener, pH adjuster and deionized water are added to an aqueous phase pot, stirred and heated, and kept warm to obtain phase B. (2) Mix phase A and phase B in an emulsifying pan and homogenize them; (3) Cool down, add the eye repair composition, homogenize, filter and discharge to obtain the multi-effect repair eye cream.

20. The method for preparing the multi-effect repair eye cream as described in claim 19, characterized in that, In step (1), the stirring speed in the preparation of phase A is 800-1200 rpm, the heating refers to raising the temperature to 80-90℃, and the holding time is 5-15 min.

21. The method for preparing the multi-effect repair eye cream as described in claim 19, characterized in that, In step (1), the stirring speed in the preparation of phase B is 800-1200 rpm, the heating refers to raising the temperature to 85-95℃, and the holding time is 10-20 min.

22. The method for preparing the multi-effect repair eye cream as described in claim 19, characterized in that, The homogenization time in step (2) is 3-7 min, and the homogenization speed is 2500-3500 rpm.

23. The method for preparing the multi-effect repair eye cream as described in claim 19, characterized in that, The cooling mentioned in step (3) refers to cooling down to 40-50℃, and the homogenization time is 10-20 min.

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