Freeze-drying device and process for preparing a transfer factor capsule

Abstract

The application relates to the technical field of medicines, and discloses a freeze-drying preparation device and process for transfer factor capsules. The freeze-drying preparation device for the transfer factor capsules comprises a crushing kettle, the side wall of the crushing kettle is fixedly connected with a mounting plate, and the top end face of the mounting plate is provided with a lifting assembly. The freeze-drying preparation device for the transfer factor capsules comprises a lifting motor, the lifting motor is fixedly connected to the bottom end face of the mounting plate, the output end of the lifting motor is fixedly connected with a threaded rod, the side wall of the threaded rod is threadedly connected with a cover body, the bottom end face of the cover body is provided with a pressing-down assembly, the pressing-down plate intermittently presses down, the tissue thrown up is hit downward again, the tissue is dropped onto the blade again due to the action of inertia, and then is crushed by the blade; the longitudinal movement of the pressing-down plate drives the mounting sleeve and the blade to move longitudinally along the rotating shaft through the transmission of the connecting sleeve, and the coverage range of the blade crushing is improved.

Description

Technical Field

[0001] This invention relates to the field of pharmaceutical technology, specifically to a freeze-drying preparation apparatus and process for transfer factor capsules. Background Technology

[0002] Transfer factor is a mixture of biologically active polypeptides and nucleotides with a molecular weight of less than 10,000 that can transmit delayed-type hypersensitivity reactions. It is characterized by its activity not being destroyed by trypsin or ribonuclease, its heat resistance, and its lack of species limitations. Currently, it is mainly used to treat viral infections and autoimmune diseases. It can bidirectionally regulate the body's immune function, exerting a dual immunomodulatory effect on the increase and decrease of cellular and humoral immunity. Simultaneously, it promotes the proliferation of T lymphocytes and regulates bone marrow hematopoietic immune function. Currently, officially marketed transfer factor products include injections, oral solutions, and transfer factor capsules.

[0003] The process for preparing transfer factor capsules typically involves pre-treating fresh tissue, which involves crushing and homogenizing the tissue, repeatedly freezing and thawing it several times, centrifuging it using a high-speed refrigerated centrifuge, collecting the centrifuged liquid, dialyzing the centrifuged liquid, collecting the dialysate, and then ultrafiltration the dialysate to obtain ultrafiltrate with a molecular weight of 6,000-10,000. Ultrafiltrates with different molecular weights are used to prepare different dosage forms of transfer factor.

[0004] However, during the actual crushing process of the aforementioned fresh tissues, due to the inherent toughness and structural complexity of the tissues, the blades often cannot completely penetrate or pierce the tissues when rotating, causing the tissues to be flung against the inner wall of the crushing container. This significantly reduces the crushing effect of the tissues, making it impossible to achieve uniform and fine cutting, thus preventing the tissues from being completely lysed and affecting the subsequent extraction and separation effects. In view of this, we propose a freeze-drying preparation device and process for transfer factor capsules. Summary of the Invention

[0005] The purpose of this invention is to provide a freeze-drying preparation apparatus and process for transfer factor capsules to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a freeze-drying preparation apparatus for transfer factor capsules, comprising a crushing vessel, wherein a mounting plate is fixedly connected to the side wall of the crushing vessel, and a lifting assembly is provided on the top end face of the mounting plate, the lifting assembly comprising:

[0007] A lifting motor is fixedly connected to the bottom end face of the mounting plate. A threaded rod is fixedly connected to the output end of the lifting motor. A cover is threadedly connected to the side wall of the threaded rod. A pressing component is provided on the bottom end face of the cover.

[0008] The pressing component includes:

[0009] A rotary motor, wherein the rotary motor is fixedly connected to the top end face of the cover, and the output end of the rotary motor is fixedly connected to a rotating shaft, and the side wall of the rotating shaft is provided with a crushing component;

[0010] The housing is fixedly connected to the bottom end face of the cover, and a reciprocating screw is rotatably connected to the inner top of the housing. A connecting piece is threaded to the side wall of the reciprocating screw.

[0011] The lower pressure plate is fixedly connected to the bottom end face of the connector;

[0012] The crushing component includes:

[0013] The mounting sleeve has a connecting sleeve fitted on its top, which is fixedly connected to the bottom end face of the lower pressure plate. The mounting sleeve is sleeved with a rotating shaft, and a blade is fixedly connected to the side wall of the mounting sleeve.

[0014] A sector-shaped plate, wherein a rotating shaft is fixedly connected to the side wall of the sector-shaped plate.

[0015] Preferably, the connector is fitted onto the inner bottom surface of the shell, and the diameter of the shell is equal to the inner diameter of the crusher.

[0016] Preferably, a pulley is fixedly connected to the side wall of the reciprocating lead screw, a pulley is fixedly connected to the side wall of the rotating shaft, and a belt is driven to the side wall of the pulley, so that the rotating shaft can rotate and drive the shaft.

[0017] Preferably, a vertical rod is fixedly connected to the top end face of the mounting plate. The vertical rod is sleeved with the side wall of the cover. The length of the threaded rod is equal to the length of the crushing component. The vertical rod limits the mounting plate so that when the lifting motor drives the threaded rod to rotate, the cover can move longitudinally. The upward movement of the cover can open the crushing vessel, allowing tissue to be placed or removed, while simultaneously rinsing the device.

[0018] Preferably, a connecting rod is fixedly connected to the side wall of the rotating shaft, and an installation ring is fixedly connected to the end of the connecting rod away from the rotating shaft. An arc-shaped plate is fixedly connected to the top end face of the installation ring, and the arc-shaped plate is movably connected to the inner wall of the crushing vessel.

[0019] Preferably, the number of arc-shaped plates is set in several groups, and the several groups of arc-shaped plates are equally spaced on the side wall of the mounting ring. The tops of the several groups of arc-shaped plates are fixedly connected by a connecting ring. The arc-shaped plates drive the tissue below to flip upward, and at the same time, the arc-shaped plates scrape the inner wall of the crushing vessel to prevent the tissue from adhering to the inner wall of the crushing vessel for a long time, thereby improving the crushing effect of the tissue.

[0020] Preferably, a guide ring is fixedly connected to the bottom end face of the lower pressure plate, and the outer wall of the guide ring is movably connected to the inner wall of the crushing vessel.

[0021] Preferably, the inner wall of the guide ring is arc-shaped, and the inner wall of the guide ring is provided with a large diameter end and a small diameter end. The small diameter end is provided on the side closer to the lower pressure plate, and the large diameter end is provided on the side away from the lower pressure plate.

[0022] Preferably, the rotating shaft includes a first cylinder, a hexagonal prism, and a second cylinder. The first cylinder is fixedly connected to the output end of the rotary motor, the hexagonal prism is fixedly connected to the bottom end of the first cylinder, and the second cylinder is fixedly connected to the bottom end of the hexagonal prism. The first cylinder is sleeved with the housing and the lower pressure plate. The hexagonal prism is sleeved with the mounting sleeve. A sector plate is fixedly connected to the side wall of the second cylinder. The sector plate is movably connected to the inner bottom of the crushing vessel. A connecting rod is fixedly connected to the side wall of the second cylinder. The rotation of the rotating shaft can drive the mounting sleeve and the blade to rotate. The blade can further crush the tissue. The rotation of the rotating shaft drives the sector plate to rotate, stirring up the tissue that has settled at the bottom, so that the tissue comes into contact with the blade again and is crushed by the blade.

[0023] A lyophilization preparation process for transfer factor capsules, the preparation process comprising the following steps:

[0024] S1. Break up fresh tissue at 10-12℃.

[0025] S2. Repeat the freeze-thaw cycle three times to further break down the cells;

[0026] S3. The tissue is centrifuged using a high-speed refrigerated centrifuge to obtain centrifuged liquid;

[0027] S4. Filter the centrifuged liquid using a filter press;

[0028] S5. Dialyze and ultrafilter the centrifuged liquid to obtain substances with a molecular weight in the range of 6000-10000;

[0029] S6. Use nanofiltration technology to concentrate the ultrafiltrate of the transfer factor to obtain a high-concentration raw material solution;

[0030] S7. Concentrate the transfer factor stock solution into powder using freeze-drying;

[0031] S8. Add excipients and additives to the powder to form small particles;

[0032] S9. Fill into capsules to make transfer factor capsules.

[0033] Compared with the prior art, the present invention provides a freeze-drying preparation apparatus and process for transfer factor capsules, which has the following beneficial effects:

[0034] 1. The freeze-drying preparation device and process for the transfer factor capsules, through the setting of lifting components, pressing components, and crushing components, intermittently presses down the lower plate, causing the thrown tissue to fall back down due to inertia and fall back onto the blade, where it is then crushed. The longitudinal movement of the lower plate, through the transmission of the connecting sleeve, drives the mounting sleeve and the blade to move longitudinally along the rotating shaft, increasing the coverage of the blade crushing. The fan-shaped plate is movably connected to the inner bottom of the crushing vessel, stirring up the tissue that has settled at the bottom, causing the tissue to re-contact the blade and be crushed, thereby improving the tissue crushing effect and thus improving the extraction effect of tissue fluid.

[0035] 2. The freeze-drying preparation device and process for the transfer factor capsules, through the setting of an arc plate, drives the tissue below to turn upward, while the arc plate scrapes the inner wall of the crushing vessel, preventing the tissue from adhering to the inner wall of the crushing vessel for a long time, thereby improving the crushing effect of the tissue.

[0036] 3. The freeze-drying preparation device and process for the transfer factor capsules, through the setting of the guide ring, scrapes the inner wall of the crushing vessel during the pressing of the lower platen, gathers the tissue attached to the inner wall of the crushing vessel to the middle, and then can be thrown back onto the blade as the lower platen presses down, thereby improving the crushing effect on the tissue. Attached Figure Description

[0037] Figure 1 This is a schematic diagram of the main structure of the present invention;

[0038] Figure 2 This is a schematic cross-sectional view of the main body of the present invention;

[0039] Figure 3 This is a schematic diagram of the cross-sectional structure of the shell of the present invention;

[0040] Figure 4 For the present invention Figure 3 Schematic diagram of the structure of region A in the middle;

[0041] Figure 5 This is a schematic diagram of the lower pressure plate structure of the present invention;

[0042] Figure 6 This is a schematic diagram of the crushing component structure of the present invention;

[0043] Figure 7 This is a schematic diagram of the cross-sectional structure of the lower pressure plate of the present invention;

[0044] Figure 8 This is a schematic diagram of the arc-shaped plate structure of the present invention.

[0045] In the diagram: 1. Crusher; 2. Mounting plate; 3. Lifting assembly; 301. Lifting motor; 302. Threaded rod; 304. Vertical rod; 305. Cover; 4. Pressing assembly; 401. Rotary motor; 402. Rotating shaft; 403. Housing; 404. Reciprocating screw; 405. Belt; 406. Connecting piece; 407. Pressing plate; 5. Crushing assembly; 501. Connecting sleeve; 502. Mounting sleeve; 503. Blade; 504. Sector plate; 6. Connecting rod; 7. Mounting ring; 8. Arc plate; 9. Guide ring. Detailed Implementation

[0046] like Figures 1-8 As shown, the present invention provides a technical solution: a freeze-drying preparation device for transfer factor capsules, including a crushing vessel 1, an installation plate 2 fixedly connected to the side wall of the crushing vessel 1, and a lifting component 3 provided on the top end face of the installation plate 2, the lifting component 3 including a lifting motor 301, a threaded rod 302, a vertical rod 304, and a cover 305.

[0047] In one embodiment of the present invention, a lifting motor 301 is fixedly connected to the bottom end face of the mounting plate 2. A threaded rod 302 is fixedly connected to the output end of the lifting motor 301. A cover 305 is threadedly connected to the side wall of the threaded rod 302. A vertical rod 304 is fixedly connected to the top end face of the mounting plate 2. The vertical rod 304 is sleeved with the side wall of the cover 305. The length of the threaded rod 302 is equal to the length of the crushing component 5. The vertical rod 304 limits the mounting plate 2 so that when the lifting motor 301 drives the threaded rod 302 to rotate, the cover 305 can move longitudinally. The cover 305 moves upward to open the crushing vessel 1, so that tissue can be placed or removed, and the device can be rinsed at the same time. A pressing component 4 is provided on the bottom end face of the cover 305.

[0048] The pressing assembly 4 includes a rotary motor 401, a rotating shaft 402, a housing 403, a reciprocating lead screw 404, a belt 405, a connecting piece 406, and a pressing plate 407.

[0049] The rotary motor 401 is fixedly connected to the top end face of the cover 305, and the output end of the rotary motor 401 is fixedly connected to the rotating shaft 402. The side wall of the rotating shaft 402 is provided with the crushing component 5.

[0050] The housing 403 is fixedly connected to the bottom end face of the cover 305. A reciprocating screw 404 is rotatably connected to the inner top of the housing 403. A pulley is fixedly connected to the side wall of the reciprocating screw 404. A pulley is fixedly connected to the side wall of the rotating shaft 402. A belt 405 is driven to drive the rotating shaft 402 to rotate. A connecting piece 406 is threadedly connected to the side wall of the reciprocating screw 404. The connecting piece 406 is sleeved with the inner bottom surface of the housing 403. The diameter of the housing 403 is equal to the inner diameter of the crusher 1. The lower pressure plate 407 is fixedly connected to the bottom end face of the connecting piece 406.

[0051] The crushing component 5 includes a connecting sleeve 501, a mounting sleeve 502, a blade 503, and a sector plate 504.

[0052] The top of the mounting sleeve 502 is fitted with a connecting sleeve 501, which is fixedly connected to the bottom end face of the lower pressure plate 407. The mounting sleeve 502 is fitted with the rotating shaft 402, and the side wall of the mounting sleeve 502 is fixedly connected with a blade 503.

[0053] Specifically, the freeze-drying preparation process of transfer factor capsules includes the following steps:

[0054] S1. Break up fresh tissue at 10-12℃.

[0055] S2. Repeat the freeze-thaw cycle three times to further break down the cells;

[0056] S3. The tissue is centrifuged using a high-speed refrigerated centrifuge to obtain centrifuged liquid;

[0057] S4. Filter the centrifuged liquid using a filter press;

[0058] S5. Dialyze and ultrafilter the centrifuged liquid to obtain substances with a molecular weight in the range of 6000-10000;

[0059] S6. Use nanofiltration technology to concentrate the ultrafiltrate of the transfer factor to obtain a high-concentration raw material solution;

[0060] S7. Concentrate the transfer factor stock solution into powder using freeze-drying;

[0061] S8. Add excipients and additives to the powder to form small particles;

[0062] S9. Fill into capsules to make transfer factor capsules.

[0063] A rotating shaft 402 is fixedly connected to the side wall of the sector plate 504. The rotating shaft 402 includes a cylinder 1, a hexagonal prism, and a cylinder 2. Cylinder 1 is fixedly connected to the output end of the rotary motor 401. The hexagonal prism is fixedly connected to the bottom end of cylinder 1. Cylinder 2 is fixedly connected to the bottom end of the hexagonal prism. Cylinder 1 is sleeved with the housing 403 and the lower pressure plate 407. The hexagonal prism is sleeved with the mounting sleeve 502. The sector plate 504 is fixedly connected to the side wall of cylinder 2. The sector plate 504 is movably connected to the inner bottom of the crushing vessel 1. A connecting rod 6 is fixedly connected to the side wall of cylinder 2. The rotation of the rotating shaft 402 can drive the mounting sleeve 502 and the blade 503 to rotate. The blade 503 can further crush the tissue. The rotation of the rotating shaft 402 drives the sector plate 504 to rotate, stirring up the tissue that has settled at the bottom, so that the tissue comes into contact with the blade 503 again and is crushed by the blade 503.

[0064] A connecting rod 6 is fixedly connected to the side wall of the rotating shaft 402. An installation ring 7 is fixedly connected to the end of the connecting rod 6 away from the rotating shaft 402. An arc-shaped plate 8 is fixedly connected to the top end face of the installation ring 7. The arc-shaped plate 8 is movably connected to the inner wall of the crushing vessel 1. Several sets of arc-shaped plates 8 are set at equal intervals on the side wall of the installation ring 7. The tops of the several sets of arc-shaped plates 8 are fixedly connected by the connecting ring. The arc-shaped plate 8 drives the tissue below to flip upward. At the same time, the arc-shaped plate 8 scrapes the inner wall of the crushing vessel 1 to prevent the tissue from adhering to the inner wall of the crushing vessel 1 for a long time, thereby improving the crushing effect of the tissue.

[0065] A guide ring 9 is fixedly connected to the bottom end face of the lower pressure plate 407. The outer wall of the guide ring 9 is movably connected to the inner wall of the crusher 1. The inner wall of the guide ring 9 is arc-shaped and has a large diameter end and a small diameter end. The small diameter end is located on the side close to the lower pressure plate 407, and the large diameter end is located on the side away from the lower pressure plate 407.

[0066] When the lifting motor 301 drives the threaded rod 302 to rotate, the cover 305 can move longitudinally. The cover 305 moves upward to open the crushing vessel 1, so that tissue can be placed or removed. At the same time, the device can be rinsed by lifting the cover 305.

[0067] The rotary motor 401 starts and drives the rotating shaft 402 to rotate. The rotation of the rotating shaft 402 drives the mounting sleeve 502 and the blade 503 to rotate. The blade 503 breaks the tissue. During the cutting process, the cells and tissues are compressed, stretched or torn by mechanical force, causing the cell fluid to flow out.

[0068] The rotation of the shaft 402 drives the reciprocating screw 404 to rotate via the belt 405. The rotation of the reciprocating screw 404 drives the connecting piece 406 and the lower pressure plate 407 to move longitudinally back and forth. The lower pressure plate 407 presses down intermittently, knocking the thrown tissue back down. Due to inertia, this part of the tissue falls back onto the blade 503 and is then crushed by the blade 503. The longitudinal movement of the lower pressure plate 407, through the transmission of the connecting sleeve 501, drives the mounting sleeve 502 and the blade 503 to move longitudinally along the shaft 402, increasing the crushing coverage of the blade 503.

[0069] The rotation of the shaft 402 drives the sector plate 504 to rotate. The sector plate 504 is movably connected to the inner bottom of the crushing vessel 1, stirring up the tissue that has settled at the bottom, so that the tissue comes into contact with the blade 503 again and is then crushed by the blade 503, thereby improving the tissue crushing effect and thus improving the extraction effect of tissue fluid.

[0070] To promote thorough tissue crushing, a connecting rod 6 is fixedly connected to the side wall of the rotating shaft 402. An installation ring 7 is fixedly connected to the end of the connecting rod 6 away from the rotating shaft 402. An arc-shaped plate 8 is fixedly connected to the top end face of the installation ring 7. The arc-shaped plate 8 is movably connected to the inner wall of the crushing vessel 1. Several sets of arc-shaped plates 8 are arranged at equal intervals on the side wall of the installation ring 7. The tops of the sets of arc-shaped plates 8 are fixedly connected by the connecting ring. The arc-shaped plates 8 cause the tissue below to flip upwards. At the same time, the arc-shaped plates 8 scrape the inner wall of the crushing vessel 1 to prevent the tissue from adhering to the inner wall of the crushing vessel 1 for a long time, thereby improving the tissue crushing effect.

[0071] In addition, to further improve the tissue crushing effect, a guide ring 9 is fixedly connected to the bottom end face of the lower pressure plate 407. The outer wall of the guide ring 9 is movably connected to the inner wall of the crushing vessel 1. The inner wall of the guide ring 9 is arc-shaped and has a large diameter end and a small diameter end. The small diameter end is located on the side closer to the lower pressure plate 407, and the large diameter end is located on the side away from the lower pressure plate 407. During the downward pressing of the lower pressure plate 407, the guide ring 9 scrapes the inner wall of the crushing vessel 1, gathering the tissue attached to the inner wall of the crushing vessel 1 to the middle. Then, with the downward pressing action of the lower pressure plate 407, it can be thrown back onto the blade 503, thereby improving the tissue crushing effect.

[0072] In this invention, during use, the lifting motor 301 drives the cover 305 to move longitudinally to open the crushing vessel 1, allowing tissue to be placed or removed. The rotating motor 401 drives the blade 503 to rotate, and the blade 503 moves longitudinally along the rotating shaft 402 to crush the tissue. The lower pressure plate 407 presses down intermittently, knocking the thrown tissue back down and then crushing it by the blade 503. The arc plate 8 causes the tissue below to flip upward, and at the same time, the arc plate 8 scrapes the inner wall of the crushing vessel 1 to prevent the tissue from adhering to the inner wall of the crushing vessel 1 for a long time.

[0073] The present invention has been described in detail above. However, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, any modifications or improvements that do not depart from the spirit of the present invention are within the scope of protection of the present invention.

Claims

1. A freeze-drying preparation apparatus for transfer factor capsules, comprising a crushing vessel (1), wherein a mounting plate (2) is fixedly connected to the side wall of the crushing vessel (1), characterized in that: The top end face of the mounting plate (2) is provided with a lifting component (3), the lifting component (3) including: A lifting motor (301) is fixedly connected to the bottom end face of the mounting plate (2). A threaded rod (302) is fixedly connected to the output end of the lifting motor (301). A cover (305) is threadedly connected to the side wall of the threaded rod (302). A pressing component (4) is provided on the bottom end face of the cover (305). The pressing component (4) includes: A rotary motor (401) is fixedly connected to the top end face of the cover (305). The output end of the rotary motor (401) is fixedly connected to a rotating shaft (402). A crushing component (5) is provided on the side wall of the rotating shaft (402). A vertical rod (304) is fixedly connected to the top end face of the mounting plate (2). The vertical rod (304) is sleeved with the side wall of the cover (305). The length of the threaded rod (302) is equal to the length of the crushing component (5). The housing (403) is fixedly connected to the bottom end face of the cover (305). The inner top of the housing (403) is rotatably connected to a reciprocating screw (404). A pulley is fixedly connected to the side wall of the reciprocating screw (404). A pulley is fixedly connected to the side wall of the rotating shaft (402). A belt (405) is drivenly connected to the side wall of the pulley. A connecting piece (406) is threadedly connected to the side wall of the reciprocating screw (404). The connecting piece (406) is sleeved with the inner bottom surface of the housing (403). The diameter of the housing (403) is equal to the inner diameter of the crusher (1). The lower pressure plate (407) is fixedly connected to the bottom end face of the connector (406). A guide ring (9) is fixedly connected to the bottom end face of the lower pressure plate (407). The outer wall of the guide ring (9) is movably connected to the inner wall of the crusher (1). The inner wall of the guide ring (9) is arc-shaped. The inner wall of the guide ring (9) is provided with a large diameter end and a small diameter end. The small diameter end is provided on the side close to the lower pressure plate (407), and the large diameter end is provided on the side away from the lower pressure plate (407). The crushing component (5) includes: Mounting sleeve (502), the top of which is fitted with a connecting sleeve (501), the connecting sleeve (501) is fixedly connected to the bottom end face of the lower pressure plate (407), the mounting sleeve (502) is fitted with the rotating shaft (402), and the side wall of the mounting sleeve (502) is fixedly connected with a blade (503). A fan-shaped plate (504) has a rotating shaft (402) fixedly connected to its side wall. A connecting rod (6) is fixedly connected to the side wall of the rotating shaft (402). The rotating shaft (402) includes a first cylinder, a hexagonal prism, and a second cylinder. The first cylinder is fixedly connected to the output end of a rotary motor (401). The hexagonal prism is fixedly connected to the bottom end of the first cylinder. The second cylinder is fixedly connected to the bottom end of the hexagonal prism. The first cylinder is sleeved with a housing (403) and a lower pressure plate (407). The hexagonal prism is sleeved with a mounting sleeve (502). A connecting rod (6) is fixedly connected to the side wall of the second cylinder. A fan-shaped plate (504) is movably connected to the inner bottom of the crushing vessel (1). A connecting rod (6) is fixedly connected to the side wall of the cylinder. An installation ring (7) is fixedly connected to the end of the connecting rod (6) away from the rotating shaft (402). An arc-shaped plate (8) is fixedly connected to the top end face of the installation ring (7). The arc-shaped plate (8) is movably connected to the inner wall of the crushing vessel (1). Several sets of arc-shaped plates (8) are provided. Several sets of arc-shaped plates (8) are equally spaced on the side wall of the installation ring (7). The tops of several sets of arc-shaped plates (8) are fixedly connected by a fixed connecting ring.

2. A freeze-drying preparation process for transfer factor capsules, based on the freeze-drying preparation apparatus for transfer factor capsules according to claim 1, characterized in that, The preparation process includes the following steps: S1. Break up fresh tissue at 10-12°C. S2. Repeat the freeze-thaw cycle three times to further break down the cells; S3. The tissue is centrifuged using a high-speed refrigerated centrifuge to obtain centrifuged liquid; S4. Filter the centrifuged liquid using a filter press; S5. Dialyze and ultrafilter the centrifuged liquid to obtain substances with a molecular weight in the range of 6000-10000; S6. Use nanofiltration technology to concentrate the ultrafiltrate of the transfer factor to obtain a high-concentration raw material solution; S7. Concentrate the transfer factor stock solution into powder using freeze-drying; S8. Add excipients and additives to the powder to form small particles; S9. Fill into capsules to make transfer factor capsules.

Images