Halohydrin dehalogenase mutant and application thereof in biosynthesis
Highly active and highly stereoselective halohydrin dehalogenase mutants were prepared by gene mutation technology. As a biological catalyst, the kinetic splitting reaction was catalyzed, solving the problem of difficulty in synthesizing chiral 3-hydroxy-3-hydroxymethyl indole and spiroethylene oxide indole indole in the prior art, and achieving efficient and mild synthesis.
Patent Information
- Application Number
- CN202411892362.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-20
- Publication Date
- 2025-04-04
AI Technical Summary
It is difficult to efficiently synthesize chiral 3-hydroxy-3-hydroxymethyl indole and spiroethylene oxide indole compounds indole, and the chemical synthesis method has problems of harsh conditions and environmental pollution.
By using gene mutation and protein modification technology, a highly stereoselective and highly active halogenol dehalogenase mutant HheC-T134S or HheC-T134A is prepared as a biocatalyst, catalyzing the kinetic resolution reaction between nitrite and racemic spiroethylene oxide indole compounds to synthesize chiral compounds.
The efficient synthesis of chiral 3-hydroxy-3-hydroxymethyl indole and spiroethylene oxide indole was achieved, with mild reaction conditions, environmentally friendly and selective, and the synthesis of chiral compounds was achieved for the first time.
Smart Images
Figure CN119752840A_ABST