Primer group for detecting African swine fever virus based on whole genome, library building method and application

By providing ASFV whole genome primer set and library building methods suitable for second-generation sequencing and nanopore sequencing platforms, the problem of difficulty in achieving ASFV whole genome detection in the prior art is solved, and efficient and sensitive virus detection and diagnosis are achieved.

CN119753240AActive Publication Date: 2025-04-04BEIJING ZHONGKE GENE TECH CO LTD
View PDF 5 Cites 3 Cited by

Patent Information

Application Number
CN202510267091.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-03-07
Publication Date
2025-04-04
Estimated Expiration
2045-03-07

AI Technical Summary

Technical Problem

The prior art is difficult to achieve rapid diagnosis and typing of African swine fever virus (ASFV) through genome-wide detection, and cannot provide the whole genome information of the virus.

Method used

It provides a primer set based on whole-genome detection of ASFV, combined with library building methods, adapted to the second-generation sequencing platform and nanopore sequencing platform to ensure that more than 95% of the virus sequence can be obtained in low-viral content samples, and the sequencing depth can reach more than 1000X.

Benefits of technology

It realizes the efficiency and sensitivity of ASFV whole genome sequencing, can accurately detect in low-viral content samples, provide deep coverage of genomic information, and supports rapid diagnosis and typing.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN119753240A_ABST
    Figure CN119753240A_ABST
Patent Text Reader

Abstract

The invention provides a primer group for sequencing an African swine fever virus based on a whole genome, a library building method and application. The primer group comprises 182 pairs of primers, and sequences of upstream primers and downstream primers of the 182 pairs of primers are shown as SEQ ID NO.1-SEQ ID NO.364. The primer group disclosed by the invention has good specificity and sensitivity, and has a good application prospect in African swine fever virus whole genome detection and preparation of reagents or kits required by detection. The library building method provided by the invention comprises the following steps: carrying out multiple PCR amplification by using the primer group provided by the invention; the library building method is suitable for a next-generation sequencing platform and a nanopore sequencing platform, the automation degree is high, 95% or above of African swine fever virus sequences can be obtained even in a sample with the virus content as low as 10 copies / microliter, and the sequencing depth can reach 1000 X or above.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • Primer probe combination for detecting African swine fever virus and application thereof

    CN116334305A

  • Real-time detection method for African swine fever virus and porcine reproductive and respiratory syndrome virus through specific primer enrichment and nanopore sequencing

    CN116970744A

  • Primer group for whole genome targeted detection of new coronavirus and application of primer group

    CN117844979A

  • Super-multiplex PCR (polymerase chain reaction) primer group and super-multiplex PCR and nanopore sequencing method for detecting broad-spectrum pig pathogens

    CN118240972A

  • PCR (Polymerase Chain Reaction) primer group for detecting African swine fever virus based on whole genome and nanopore sequencing method based on single molecule sequencing

    CN118703709A

Cited By

  • Primer group for detecting porcine HEV whole genome, kit comprising primer group and application

    CN121137260A

  • Primers and method for sequencing whole genome of African swine fever virus based on next-generation sequencing platform and targeted enrichment

    CN122128476A

  • Primer set, amplification methods, sequencing methods, and their applications for ALV whole genome amplification

    CN122669148A