Conservative B cell epitope peptide based on gE protein and application of B cell epitope peptide in diagnosis of porcine pseudorabies virus antibody

Abstract

The invention discloses a conservative B cell epitope peptide on surface envelope glycoprotein of porcine pseudorabies virus (PRV) and application of the conservative B cell epitope peptide in PRV antibody diagnosis. The sequence of the PRV gE conservative linear B cell epitope is GPGGGD, and the PRV gE conservative linear B cell epitope is named as GD-GG. The B cell epitope can be subjected to functional exhibition expression on the surface of an inert carrier S9H thallus, only a PRV infection antibody is specifically detected by a GD-GG-antibody direct mediated agglutination test detection method established on the basis of GD-GG subjected to exhibition expression on the surface of the S9H thallus, and the B cell epitope has no cross reaction with other porcine infectious disease antibodies. And if two amino acids GG in the GD-GG sequence are mutated into RR, namely GPGGGD is mutated into GPRRGD, the specific binding reaction with the PRV infected antibody is avoided. The diagnosis method has the advantages of high specificity, convenience in operation, intuitive result judgment and the like, and is expected to become a technical means for accurate and visual diagnosis of PRV infection.

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