Magnetic bead mixing device

Abstract

A magnetic bead blending device comprises a supporting mechanism (100), a driving mechanism (200) and a reagent bottle fixing piece (300), the supporting mechanism (100) is provided with a containing cavity and an opening, the center line of the containing cavity extends along a first straight line (20), an output shaft (201) of the driving mechanism (200) can rotate around the first straight line (20), and the reagent bottle fixing piece (300) is arranged to be used for fixing a reagent bottle (10) containing magnetic beads. The reagent bottle fixing piece (300) is arranged in the containing cavity through the opening, the bottom end of the reagent bottle fixing piece (300) is in transmission connection with the output shaft (201), and the central axis of the reagent bottle fixing piece (300) extends along the second straight line (30). Under the driving of the driving mechanism (200), the reagent bottle fixing piece (300) can swing in the mode that the second straight line (30) moves around the first straight line (20) in the circumferential direction, in the swinging process, the included angle between the second straight line (30) and the first straight line (20) is alpha degrees all the time, and the moving track of the second straight line (30) is a conical surface.

Description

Magnetic bead mixing device Technical Field

[0001] The present application relates to the technical field of biological sample extraction, for example, to a magnetic bead mixing device. Background Art

[0002] Magnetic bead extraction technology is widely used in the extraction and purification of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Before using magnetic beads to extract and purify DNA or RNA from biological samples, the beads and solution in the reagent bottle must be mixed thoroughly to allow the silanol groups on the bead surface to specifically bind to the nucleic acids in the solution through hydrogen bonds and electrostatic interactions. Under high-salt conditions, the magnetic beads can bind to nucleic acids, while in low-salt environments, the bound nucleic acids can be eluted from the beads. This principle allows for the direct isolation of nucleic acids from complex biological systems.

[0003] The automated magnetic bead mixing method used in gene sequencing primarily relies on pipetting. This involves first adding magnetic beads to a sample-filled reagent bottle, then repeatedly aspirating and flushing the beads using a pipette and injection needle to achieve elution and mixing. However, when this method is applied to long-fragment libraries for gene sequencing, magnetic beads clumping is more severe because the magnetic beads attached to the side of the reagent bottle are difficult to flush away during the pipetting mixing process. This makes elution and mixing relatively difficult.

[0004] Summary of the Invention

[0005] The present application provides a magnetic bead mixing device, which prevents magnetic beads from agglomerating on the side wall of a reagent bottle, thereby improving the effect and efficiency of biological sample extraction.

[0006] The present application provides a magnetic bead mixing device, comprising: a supporting mechanism, wherein the supporting mechanism has a accommodating cavity, the accommodating cavity forms an opening at the top of the supporting mechanism, and the center line of the accommodating cavity extends along a first straight line; a driving mechanism, wherein the driving mechanism includes an output shaft, and the output shaft can rotate around the first straight line; a reagent bottle fixing member, wherein the reagent bottle fixing member is configured to fix a reagent bottle containing magnetic beads, the reagent bottle fixing member is placed in the accommodating cavity through the opening, and the bottom end of the reagent bottle fixing member is transmission-connected to the output shaft, the central axis of the reagent bottle fixing member extends along a second straight line, and the second straight line and the first straight line are intersecting; wherein, under the drive of the driving mechanism, the reagent bottle fixing member can swing in a manner that the second straight line moves circumferentially around the first straight line, and during the swinging process, the angle between the second straight line and the first straight line is always α°, and the moving trajectory of the second straight line is a conical surface. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] FIG1 is a schematic structural diagram of a magnetic bead mixing device provided in an embodiment of the present application;

[0008] Figure 2 is an enlarged view of portion B in Figure 1;

[0009] FIG3 is a partially cutaway schematic diagram of a magnetic bead mixing device provided in an embodiment of the present application;

[0010] FIG4 is a cross-sectional view of a magnetic bead mixing device provided in an embodiment of the present application;

[0011] FIG5 is a schematic diagram of an eccentric drive shaft of a magnetic bead mixing device provided in an embodiment of the present application.

[0012] In the picture:

[0013] 100, support mechanism; 101, base; 102, support column; 103, motor base; 104, bearing seat; 105, bearing end cover; 106, first chamber; 107, second chamber;

[0014] 200, driving mechanism; 201, output shaft;

[0015] 300, reagent bottle fixing part; 301, test tube cavity; 302, tubular portion; 303, tapered portion; 304, connecting column;

[0016] 400, eccentric drive shaft; 401, plug hole; 402, mounting hole; 403, sensor plate;

[0017] 500, limiting mechanism; 501, first limiting member; 502, second limiting member;

[0018] 600, third limiter;

[0019] 700, first joint bearing;

[0020] 800, second spherical bearing;

[0021] 900, induction sensor;

[0022] 110. Deep groove ball bearings;

[0023] 120, circlip;

[0024] 10. Reagent bottle; 20. First straight line; 30. Second straight line. DETAILED DESCRIPTION

[0025] The technical solution of the present application will be described below in conjunction with the accompanying drawings, and the described embodiments are part of the embodiments of the present application.

[0026] In the description of this application, terms such as "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer" indicate positions or locations based on those shown in the accompanying drawings. These terms are intended solely to facilitate the description of this application and simplify the description. They are not intended to indicate or imply that the devices or components referred to must have, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed to indicate or imply relative importance. The terms "first position" and "second position" refer to two distinct positions.

[0027] In the description of this application, unless otherwise specified or limited, the terms "mounted," "connected," and "connected" should be understood broadly. For example, they can refer to fixed or detachable connections; mechanical or electrical connections; direct or indirect connections through an intermediate medium; and internal communication between two components. A person of ordinary skill in the art will understand the meaning of these terms in this application based on the circumstances.

[0028] The present application provides a magnetic bead mixing device, which is configured to mix the magnetic beads in a reagent bottle to prevent the magnetic beads from aggregating on the side walls of the reagent bottle, thereby affecting the extraction and purification of biological macromolecules such as DNA and RNA in biological samples.

[0029] As shown in Figures 1 to 4, the magnetic bead mixing device includes a supporting mechanism 100, a driving mechanism 200 and a reagent bottle fixture 300. The supporting mechanism 100 is the mounting structure of the entire magnetic bead mixing device, and the driving mechanism 200 and the reagent bottle fixture 300 are both mounted on the supporting mechanism 100. The supporting mechanism 100 has a accommodating cavity, which forms an opening at the top of the supporting mechanism 100, and the center line of the accommodating cavity extends along the first straight line 20. In some embodiments, the first straight line 20 is vertically arranged. The driving mechanism 200 is the power component of the entire magnetic bead mixing device, and the driving mechanism 200 includes an output shaft 201, which can rotate with the first straight line 20 as the rotation axis. The reagent bottle fixing part 300 is a component for fixing the reagent bottle 10. The reagent bottle fixing part 300 is placed in the accommodating cavity through the opening, and the bottom end of the reagent bottle fixing part 300 is transmission-connected to the output shaft 201. The central axis of the reagent bottle fixing part 300 extends along the second straight line 30, and the second straight line 30 and the first straight line 20 are intersected, that is, the second straight line 30 and the first straight line 20 have an intersection.

[0030] Driven by the driving mechanism 200, the reagent bottle fixing member 300 can swing in a manner such that the second straight line 30 moves circumferentially around the first straight line 20. During the swinging process of the reagent bottle fixing member 300, the angle between the second straight line 30 and the first straight line 20 is always α°, and the movement trajectory of the second straight line 30 is a conical surface. Since the reagent bottle 10 is fixed on the reagent bottle fixing member 300, the reagent bottle 10 and the sample and magnetic beads in the reagent bottle 10 can all swing. During the swinging process of the reagent bottle 10, a centrifugal force is generated in the reagent bottle 10. Under the action of this centrifugal force, the magnetic beads can be shaken and mixed with the sample, and the magnetic beads cannot condense on the side wall of the reagent bottle 10, thereby improving the effect and efficiency of biological sample extraction.

[0031] In some embodiments, α° is 1°, 2°, or 3°. This angle can prevent the magnetic beads from gathering on the sidewalls of the reagent bottle 10, and can also prevent the sample in the reagent bottle 10 from spilling out of the opening of the reagent bottle 10 under the action of centrifugal force. In other embodiments of the present application, the value of α° can be flexibly set according to needs.

[0032] Continuing to refer to shown in Figures 2 to 5, the magnetic bead mixing device also includes an eccentric drive shaft 400, which is utilized to realize the transmission connection between the output shaft 201 of the reagent bottle fixing member 300 and the driving mechanism 200. The eccentric drive shaft 400 is placed in the accommodating chamber, and the bottom end of the eccentric drive shaft 400 is connected to the output shaft 201. The eccentric drive shaft 400 can rotate around the first straight line 20 under the drive of the driving mechanism 200. The top of the eccentric drive shaft 400 is provided with a mounting hole 402, and the spacing between the central axis of the mounting hole 402 and the first straight line 20 is A. The bottom end of the reagent bottle fixing member 300 is swingably connected in the mounting hole 402. In an embodiment of the present application, the value of spacing A is not limited and can be flexibly set according to demand.

[0033] In some embodiments, the accommodating chamber includes a first chamber 106 and a second chamber 107. The eccentric drive shaft 400 is rotatably disposed within the first chamber 106. A deep groove ball bearing 110 is disposed between the outer wall of the eccentric drive shaft 400 and the inner wall of the first chamber 106. In some embodiments, to limit the position of the deep groove ball bearing 110, a stepped surface is formed on the inner wall of the first chamber 106, and a shoulder is formed on the outer wall of the eccentric drive shaft 400. The deep groove ball bearing 110 is limited between the stepped surface and the shoulder.

[0034] In some other embodiments, in addition to using an eccentric drive shaft 400 to achieve a transmission connection between the reagent bottle fixture 300 and the output shaft 201 of the drive mechanism 200, a planetary gear mechanism can also be used to achieve a transmission connection between the reagent bottle fixture 300 and the output shaft 201 of the drive mechanism 200, the sun gear of the planetary gear mechanism is connected to the output shaft 201 of the drive mechanism 200, and the planetary gear of the planetary gear mechanism is swingably connected to the bottom end of the reagent bottle fixture 300.

[0035] Continuing with reference to Figures 3 to 4, in order to enable the eccentric drive shaft 400 to swing accurately according to the set angle, the magnetic bead mixing device also includes a first joint bearing 700, the first joint bearing 700 including an outer ring and an inner ring, the outer ring having an inner spherical surface, the inner ring having an outer spherical surface, and the inner ring being swingably placed inside the outer ring. The first joint bearing 700 is placed in the mounting hole 402, the outer ring of the first joint bearing 700 is fixedly arranged relative to the mounting hole 402, and the central axis of the outer ring of the first joint bearing 700 coincides with the central axis of the mounting hole 402. The bottom end of the reagent bottle fixture 300 is formed with a connecting column 304, which is inserted into the inner ring of the first joint bearing 700.

[0036] Continuing with Figure 3 , the magnetic bead mixing device further includes a second spherical plain bearing 800, which includes an outer ring and an inner ring. The outer ring has an inner spherical surface, the inner ring has an outer spherical surface, and the inner ring is swingably positioned within the outer ring. The second spherical plain bearing 800 is positioned within the accommodating cavity and positioned near the opening. The outer ring of the second spherical plain bearing 800 is fixedly positioned within the accommodating cavity, and the central axis of the outer ring of the second spherical plain bearing 800 coincides with the first straight line 20. The reagent bottle fixture 300 is positioned through the inner ring of the second spherical plain bearing 800. The central axis of the inner ring of the first spherical plain bearing 700 and the central axis of the inner ring of the second spherical plain bearing 800 always coincide with the second straight line 30.

[0037] 2 , the magnetic bead mixing device further includes a limiting mechanism 500, which is configured to limit the reagent bottle fixture 300 so that the reagent bottle fixture 300 can only swing. The limiting mechanism 500 includes a first limiting member 501 and a second limiting member 502, wherein the first limiting member 501 is disposed on the top surface of the support mechanism 100, and the second limiting member 502 is disposed on the outer wall surface of the reagent bottle fixture 300. The second limiting member 502 cooperates with the first limiting member 501 to limit the rotation of the reagent bottle fixture 300 around the second straight line 30.

[0038] In some embodiments, the first limiting member 501 is a limiting plate, and a limiting groove is provided on the limiting plate. For example, the limiting plate is a U-shaped plate, and the U-shaped plate surface of the U-shaped plate is perpendicular to the radial arrangement of the reagent bottle fixing member 300, the top of the U-shaped groove is open, and the U-shaped groove passes through the limiting plate in the radial direction of the reagent bottle fixing member 300. The second limiting member 502 includes a limiting shaft and a limiting bearing, the limiting shaft is protruded along the radial direction of the reagent bottle fixing member 300 outside the reagent bottle fixing member 300, the limiting bearing is sleeved on the limiting shaft, and the limiting bearing is movably placed in the limiting groove. Since the inner wall of the U-shaped groove has a certain limiting effect on the limiting bearing, the limiting bearing placed in the U-shaped groove can rotate around the limiting shaft and can also swing up and down in the U-shaped groove, but cannot be moved out of the U-shaped groove.

[0039] In some embodiments, the second position-limiting member 502 is a cam follower, which is a standard part that can be purchased directly and is easy to use.

[0040] Continuing with reference to Figures 1, 3, 4 and 5, the magnetic bead mixing device also includes a detection mechanism, which includes a sensing sheet 403 and a sensing sensor 900. The sensing sheet 403 is disposed on the eccentric drive shaft 400, and the sensing sensor 900 is disposed on the supporting mechanism 100. When the driving mechanism 200 is reset to the origin, the sensing sensor 900 detects the sensing sheet 403.

[0041] In some embodiments, as shown in Figures 4 and 5, the sensing piece 403 is directly integrated into the eccentric drive shaft 400. The sensing piece 403 is an annular convex edge formed on the outer wall surface of the eccentric drive shaft 400, and a through groove is provided at the outer edge of the annular convex edge. The inductive sensor 900 includes a transmitting end and a receiving end that are relatively arranged. The transmitting end and the receiving end are respectively arranged on the upper and lower sides of the annular convex edge and are arranged opposite to the position of the through groove. When the through groove rotates to between the transmitting end and the receiving end, the light emitted by the transmitting end can be received by the receiving end, which means that the drive mechanism 200 has reset to the origin; and when the through groove rotates to other positions, the other parts of the annular convex edge block the light emitted by the transmitting end, making it impossible for the receiving end to receive it, which means that the drive mechanism 200 has not reset to the origin. The inductive sensor 900 and the sensing piece 403 can count the number of rotations of the output shaft 201 of the drive mechanism 200, thereby facilitating the control of the swing duration of the reagent bottle 10.

[0042] In some parallel embodiments, the sensing plate 403 and the eccentric drive shaft 400 are two independent components. The sensing plate 403 may be an annular structure, so that the sensing plate 403 can be sleeved on the eccentric drive shaft 400 and fixed. The fixing method can be selected from welding, connecting with a connector, or clamping. The sensing plate 403 can also be a circular plate. The sensing plate 403 is fixedly connected to the end surface of the eccentric drive shaft 400, such as the upper end surface or the lower end surface. The fixing method of the sensing plate 403 and the eccentric drive shaft 400 includes, but is not limited to, welding, connecting with a connector, or clamping.

[0043] In some embodiments, the driving mechanism 200 includes a closed-loop motor, and the motor shaft of the closed-loop motor is the output shaft 201. The closed-loop motor can implement closed-loop control, thereby achieving high-speed and high-precision control.

[0044] In some parallel embodiments, the driving mechanism 200 includes a stepper motor, and the motor shaft of the stepper motor is the output shaft 201 .

[0045] In some parallel embodiments, the driving mechanism 200 includes a stepper motor and a synchronous belt mechanism, the motor shaft of the stepper motor is connected to the driving pulley of the synchronous belt mechanism, and the driven pulley shaft of the synchronous belt mechanism is the output shaft 201.

[0046] 4 , the reagent bottle holder 300 defines a test tube cavity 301 along the second straight line 30, and the reagent bottle 10 is inserted into the test tube cavity 301. This arrangement ensures that the sample in the reagent bottle 10 does not need to be touched during the mixing process, thereby avoiding the occurrence of sample cross contamination.

[0047] The reagent bottle 10 includes a conical body and a cap. The body is connected to the opening of the body via a flexible connector. To prevent the reagent bottle 10 placed within the test tube cavity 301 from accidentally escaping from the test tube cavity 301, as shown in Figure 2, the reagent bottle fixture 300 is provided with a third retainer 600. The third retainer 600 is configured to engage the cap of the reagent bottle 10. In some embodiments, the third retainer 600 is a U-shaped piece, and the connector on the cap is engaged with the U-shaped groove of the U-shaped piece.

[0048] In some embodiments, the support mechanism 100 is a multi-component structure. Continuing with FIG1 , the support structure includes a base 101, a support column 102, a motor base 103, a bearing base 104, and a bearing end cap 105, connected sequentially from bottom to top. Connection methods include, but are not limited to, welding, connector connection, or magnetic attraction.

[0049] In some embodiments, the base 101 is a rectangular plate. There are multiple support columns 102, and the multiple support columns 102 are evenly distributed between the base 101 and the motor base 103, and a space for installing the drive mechanism 200 is formed between the multiple support columns 102. In some embodiments, there are four support columns 102, and the four support columns 102 are respectively arranged at the four corners of the base 101. The number of support columns 102 can be flexibly adjusted according to demand. A first chamber 106 in a stepped shape is provided in the motor base 103, and a second chamber 107 connected to the first chamber 106 is provided in the bearing seat 104, and the second chamber 107 is also stepped. A part of the structure of the eccentric drive shaft 400 is placed in the first chamber 106, and the other part is placed in the second chamber 107. The deep groove ball bearing 110 is placed in the first chamber 106, and the output shaft 201 of the drive mechanism 200 extends into the first chamber 106 and is connected to the bottom end of the eccentric drive shaft 400. The bearing end cap 105 is connected to the top of the bearing seat 104. The second spherical plain bearing 800 is placed in the second chamber 107 and close to the opening. The outer ring of the second spherical plain bearing 800 is limited between the stepped surface of the second chamber 107 and the bearing end cap 105. The bottom end of the inner ring of the second spherical plain bearing 800 is limited by the retaining ring 120 mounted on the reagent bottle fixture 300. The bearing end cap 105 is an annular structure, and its inner annular surface forms the opening of the support mechanism 100.

[0050] Example:

[0051] The following describes a magnetic bead mixing device according to an embodiment of the present application with reference to FIG1 to FIG5 .

[0052] As shown in Figures 1 to 5, the magnetic bead mixing device includes a supporting mechanism 100, a driving mechanism 200, a reagent bottle fixing member 300, an eccentric driving shaft 400, a first joint bearing 700, a second joint bearing 800, a deep groove ball bearing 110, a limiting mechanism 500 and a detection mechanism.

[0053] The support mechanism 100 includes a base 101, a support column 102, a motor base 103, a bearing seat 104, and a bearing end cover 105, which are connected in sequence from bottom to top. The base 101 is a rectangular plate. The bottom end of the motor base 103 is circumferentially provided with an annular convex edge. There are four support columns 102, which are respectively arranged at the four corners of the base 101. The bottom ends of the support columns 102 are fixedly connected to the base 101, and the top ends of the support columns 102 are fixedly connected to the annular convex edge of the motor shaft. The motor base 103 and the bearing seat 104 are connected by a connecting piece. A first chamber 106 is provided vertically through the motor base 103, and a second chamber 107 is provided vertically through the bearing seat 104. The first chamber 106 and the second chamber 107 are connected and are both stepped chambers. Bearing end cap 105 is a rectangular ring plate with a circular opening extending therethrough. Bearing end cap 105 is mounted on bearing seat 104 and partially blocks the top opening of second chamber 107. The first chamber 106, the second chamber 107, and the inner bore of bearing end cap 105 form the accommodating chamber of support mechanism 100, with the central axis of the accommodating chamber being first line 20.

[0054] The driving mechanism 200 is a closed-loop motor, which is arranged between the base 101 and the motor seat 103 and located between the four support columns 102. The motor shaft of the closed-loop motor is the output shaft 201 of the driving mechanism 200, and the motor shaft of the closed-loop motor extends into the first chamber 106.

[0055] The eccentric drive shaft 400 is rotatably connected to the housing cavity of the support mechanism 100 via a deep groove ball bearing 110. The central axis of the eccentric drive shaft 400 is the second straight line 30, and the angle between the second straight line 30 and the first straight line 20 is α°. The top end of the eccentric drive shaft 400 is placed in the second chamber 107 of the bearing seat 104, and the bottom end of the eccentric drive shaft 400 is placed in the first chamber 106 of the motor seat 103. A shoulder is formed on the outer wall of the eccentric drive shaft 400. The deep groove ball bearing 110 is placed in the first chamber 106 and sleeved on the eccentric drive shaft 400. The deep groove ball bearing 110 is vertically limited by the shoulder of the eccentric drive shaft 400 and the stepped surface in the first chamber 106. The bottom end of the eccentric drive shaft 400 is provided with a plug hole 401 along the vertical direction, and the top end of the eccentric drive shaft 400 is provided with a mounting hole 402 along the installation direction. The distance between the center axis of the plug hole 401 and the center axis of the mounting hole 402 is A. The motor shaft of the closed-loop motor is inserted into the plug hole 401 from bottom to top, and the first spherical plain bearing 700 is placed in the mounting hole 402. The first spherical plain bearing 700 includes an outer ring and an inner ring. The outer ring has an inner spherical surface, and the inner ring has an outer spherical surface. The inner ring is swingably positioned within the outer ring. The center axis of the outer ring of the first spherical plain bearing 700 coincides with the center axis of the mounting hole 402, and the center axis of the inner ring of the first spherical plain bearing 700 coincides with the second straight line 30. A second joint bearing 800 is arranged in the second chamber 107, and the reagent bottle fixing part 300 is arranged through the inner ring of the second joint bearing 800. The central axis of the outer ring of the second joint bearing 800 coincides with the first straight line 20, and the central axis of the inner ring of the second joint bearing 800 coincides with the second straight line 30. The inner ring of the second joint bearing 800 is limited by a retaining spring 120 mounted on the reagent bottle fixing part 300.

[0056] The reagent bottle fixing part 300 includes a tubular portion 302, a conical portion 303 and a connecting column 304 connected in sequence, the large-sized end of the conical portion 303 is connected to the bottom end of the tubular portion 302, and the small-sized end of the conical portion 303 is connected to the top end of the connecting column 304. The connecting column 304 is inserted into the inner ring of the first joint bearing 700. A test tube cavity 301 is provided on the tubular portion 302, and the reagent bottle 10 is inserted into the test tube cavity 301. The reagent bottle 10 includes a bottle body and a bottle cap, the bottle body is conical, and the bottle cap is connected to the opening of the bottle body by a bendable connecting piece. A third limiting member 600 is provided on the reagent bottle fixing part 300, and the third limiting member 600 is configured to clamp the bottle cap of the reagent bottle 10.

[0057] The limiting mechanism 500 includes a first limiting member 501 and a second limiting member 502. The first limiting member 501 is disposed on the top surface of the support mechanism 100 and is a U-shaped limiting plate having a U-shaped limiting groove. The U-shaped plate surface of the limiting plate is perpendicular to the radial direction of the reagent bottle holder 300. The top of the U-shaped limiting groove is open, and the limiting groove penetrates the limiting plate in the radial direction of the reagent bottle holder 300. The second limiting member 502 is disposed on the outer wall surface of the reagent bottle holder 300 and is a cam follower. The bearing of the cam follower is placed in the limiting groove.

[0058] The detection mechanism includes an induction sheet 403 and an induction sensor 900 . The induction sheet 403 is arranged on the eccentric drive shaft 400 , and the induction sensor 900 is arranged on the bearing seat 104 . When the closed motor is reset to the origin, the induction sensor 900 detects the induction sheet 403 .

Claims

1. Magnetic bead mixing device, including: A support mechanism (100), the support mechanism (100) having a receiving cavity, the receiving cavity forming an opening at the top of the support mechanism (100), the center line of the receiving cavity extending along a first straight line (20); A driving mechanism (200), the driving mechanism (200) comprising an output shaft (201), the output shaft (201) being configured to perform rotational motion around the first straight line (20); A reagent bottle fixing member (300), the reagent bottle fixing member (300) is configured to fix a reagent bottle (10) containing magnetic beads, the reagent bottle fixing member (300) is placed in the accommodating cavity through the opening, and the bottom end of the reagent bottle fixing member (300) is transmission-connected to the output shaft (201), the central axis of the reagent bottle fixing member (300) extends along a second straight line (30), and the second straight line (30) and the first straight line (20) are intersected; Wherein, under the drive of the driving mechanism (200), the reagent bottle fixing member (300) can swing in a manner that the second straight line (30) moves circumferentially around the first straight line (20). During the swinging process, the angle between the second straight line (30) and the first straight line (20) is always α°, and the movement trajectory of the second straight line (30) is a conical surface.

2. The magnetic bead mixing device according to claim 1 further includes an eccentric drive shaft (400), the eccentric drive shaft (400) is placed in the accommodating cavity, the bottom end of the eccentric drive shaft (400) is connected to the output shaft (201), and the eccentric drive shaft (400) is configured to rotate around the first straight line (20) under the drive of the driving mechanism (200), and a mounting hole (402) is provided at the top end of the eccentric drive shaft (400), and the distance between the central axis of the mounting hole (402) and the first straight line (20) is A, and the bottom end of the reagent bottle fixing member (300) is swingably connected to the mounting hole (402).

3. The magnetic bead mixing device according to claim 2, further comprising at least one of the following: A first joint bearing (700), wherein the first joint bearing (700) is placed in the mounting hole (402), and a connecting column (304) is formed at the bottom end of the reagent bottle fixing member (300), and the connecting column (304) is inserted into the inner ring of the first joint bearing (700); A second joint bearing (800) is placed in the accommodating cavity, and the reagent bottle fixing member (300) is arranged through the inner ring of the second joint bearing (800).

4. The magnetic bead mixing device according to claim 1 further includes a limiting mechanism (500), wherein the limiting mechanism (500) includes a first limiting member (501) and a second limiting member (502), wherein the first limiting member (501) is arranged on the top surface of the supporting mechanism (100), and the second limiting member (502) is arranged on the outer wall surface of the reagent bottle fixing member (300), and the second limiting member (502) cooperates with the first limiting member (501) to limit the rotation of the reagent bottle fixing member (300) around the second straight line (30).

5. The magnetic bead mixing device according to claim 4, wherein: The first limiting member (501) is a limiting plate, and a limiting slot is provided on the limiting plate; The second limiting member (502) includes a limiting shaft and a limiting bearing, wherein the limiting shaft is protruded outside the reagent bottle fixing member (300) along the radial direction of the reagent bottle fixing member (300), and the limiting bearing is sleeved on the limiting shaft, and the limiting bearing is movably placed in the limiting groove.

6. The magnetic bead mixing device according to claim 5, wherein: The second position-limiting member (502) is a cam follower.

7. The magnetic bead mixing device according to claim 2 further includes a detection mechanism, wherein the detection mechanism includes a sensing piece (403) and a sensing sensor (900), wherein the sensing piece (403) is arranged on the eccentric drive shaft (400), and the sensing sensor (900) is arranged on the supporting mechanism (100), and when the driving mechanism (200) is reset to the origin, the sensing sensor (900) detects the sensing piece (403).

8. The magnetic bead mixing device according to claim 1, wherein: The driving mechanism (200) comprises a closed-loop motor, the motor shaft of the closed-loop motor being the output shaft (201); or, The driving mechanism (200) includes a stepping motor, the motor shaft of the stepping motor being the output shaft (201); or, The driving mechanism (200) comprises a stepping motor and a synchronous belt mechanism, the motor shaft of the stepping motor is connected to the driving pulley of the synchronous belt mechanism, and the driven pulley shaft of the synchronous belt mechanism is the output shaft (201).

9. The magnetic bead mixing device according to any one of claims 1 to 8, wherein: The reagent bottle fixing member (300) is provided with a test tube cavity (301) along the second straight line (30), and the reagent bottle (10) is inserted into the test tube cavity (301).

10. The magnetic bead mixing device according to claim 9, wherein: The reagent bottle fixing member (300) is provided with a third limiting member (600), and the third limiting member (600) is configured to clamp the bottle cap of the reagent bottle (10).

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