A method for breeding high-sulforaphane content broccoli seeds

By using a specific ratio of glucoraphane synthesis promoters, ESP inhibitors, and plant growth regulators, the problem of low glucoraphane content in broccoli seeds was solved, resulting in a significant increase in glucoraphane content and improved glucoraphane yield.

CN119817405BActive Publication Date: 2026-07-21天津天隆江大生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
天津天隆江大生物科技有限公司
Filing Date
2025-02-07
Publication Date
2026-07-21

AI Technical Summary

Technical Problem

In existing technologies, broccoli seeds contain low levels of sulforaphane and have low yields of sulforaphane. Furthermore, epidermal specific sulfur protein (ESP) in broccoli seeds promotes the degradation of sulforaphane into nitrile compounds, leading to a decrease in the conversion rate of sulforaphane. Existing methods are time-consuming and have limited effectiveness.

Method used

By using a specific ratio of glucoraphane synthesis promoters, ESP inhibitors, and plant growth regulators in combination, and spraying broccoli leaves, the synthesis and accumulation of glucoraphane are promoted. This is achieved through the synergistic effect of components such as selenomethionine, chitosan oligosaccharide, tryptophan, seaweed extract, N-acetylcysteine, EDTA-2Na, gibberellin, naphthaleneacetic acid, and 6-benzylaminopurine, thereby increasing the glucoraphane content in broccoli seeds.

Benefits of technology

The study significantly increased the glucoraphane content in broccoli seeds. The synergistic effect of glucoraphane synthesis promoters and plant growth regulators effectively improved the yield of glucoraphane. The ESP inhibitor suppressed the activity of ESP, thus achieving the cultivation of broccoli seeds with high glucoraphane content.

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Abstract

The application relates to a high-sulforaphane-content broccoli seed cultivation method, which comprises the following steps: S1. After broccoli enters the flowering stage, a culture fertilizer is sprayed on the leaves of the broccoli for the first time, and then the spraying is performed every 3-4 days, and the spraying is performed for 3-4 times in total; S2. Mature broccoli seeds after cultivation for 60-65 days are collected and dried to obtain broccoli seeds rich in sulforaphane; the culture fertilizer in the step S1 comprises a sulforaphane synthesis promoter with a content of 1.2-1.6 mg / L, an ESP inhibitor with a content of 0.8-1.0 mg / L and a plant growth regulator with a content of 2.1-2.5 mg / L, and the sulforaphane synthesis promoter comprises selenomethionine, chitooligosaccharide, tryptophan and seaweed extract with a mass ratio of (5-7):(1-2):(20-30):(10-20). The application has the effect of improving the sulforaphane content in broccoli seeds.
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