Feed enriched with prebiotic and probiotic complex and its preparation process
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- GUANGDONG KONDARL AGRO PASTORAL TECH
- Filing Date
- 2025-01-09
- Publication Date
- 2026-07-21
Abstract
Description
Technical Field
[0001] This invention belongs to the technical field of feed, and relates to a feed rich in prebiotic and probiotic complex and its preparation process. Background Technology
[0002] In modern animal husbandry, the scientific formulation of feed is crucial for improving animal health and production performance. Prebiotics and probiotics, as feed additives, play a significant role in maintaining intestinal health, enhancing immunity, and promoting nutrient absorption. Prebiotics such as xylooligosaccharides, Ganoderma lucidum polysaccharides, or chitosan oligosaccharides, when added to feed, selectively promote the growth and activity of beneficial microorganisms in the gut. Probiotics, being live microorganisms, provide health benefits to the host when added to feed in sufficient quantities. The addition of these two components not only helps improve the digestive and absorptive functions of animals but also effectively prevents intestinal diseases, reduces antibiotic use, and thus improves breeding efficiency and product quality. The stability of prebiotics such as xylooligosaccharides, Ganoderma lucidum polysaccharides, or chitosan oligosaccharides in feed is affected by various factors, including feed storage conditions, feed processing, and interactions with other components in the feed. For example, xylooligosaccharides are relatively stable under acidic conditions but are prone to degradation under alkaline conditions. Therefore, pH control of the feed is essential for maintaining the stability of xylooligosaccharides. In addition, the high temperature and high pressure during feed processing may damage the structure of prebiotics and affect their biological activity. Summary of the Invention
[0003] The purpose of this invention is to provide a feed rich in prebiotic and probiotic complex and its preparation process. This invention uses Bacillus subtilis and Saccharomyces cerevisiae to ferment soybean meal, which effectively increases the content of polypeptides and other prebiotics in the fermentation products, thereby significantly improving the nutritional value of the nutritional additive. Using montmorillonite as a carrier, the soybean meal fermentation products can attach to montmorillonite, and then cysteine, cinnamaldehyde derivatives and eugenol form chemical grafts, which can significantly improve the stability of the nutritional additive and its ABTS+ free radical scavenging ability.
[0004] The objective of this invention can be achieved through the following technical solutions:
[0005] A feed rich in prebiotic and probiotic complex includes a nutrient additive and a basal feed, wherein the mass ratio of the nutrient additive to the basal feed is 0.3-0.4:100, and the nutrient additive is composed of a mixture of probiotic and prebiotic additives at a mass ratio of 2-3:30-35.
[0006] As a preferred embodiment of the present invention, the probiotics are composed of Bacillus licheniformis freeze-dried powder, Bacillus subtilis freeze-dried powder, Lactobacillus acidophilus freeze-dried powder and Saccharomyces cerevisiae freeze-dried powder in a mass ratio of 1-2:1-2:0.6-0.8:3-4.
[0007] As a preferred embodiment of the present invention, the viable bacteria concentration in the freeze-dried powders of Bacillus licheniformis, Bacillus subtilis, Lactobacillus acidophilus, and Saccharomyces cerevisiae is 5 × 10⁻⁶. 9 -2×10 12 per g.
[0008] As a preferred embodiment of the present invention, the preparation method of the prebiotic additive includes the following steps:
[0009] Step 1: Crush, grind, sieve, sterilize, add deionized water and stir to mix to obtain soybean meal base liquid;
[0010] Step 2: Mix the soybean meal base liquid and the mixed microbial agent, ferment, sterilize with ultraviolet light, and dry to obtain the fermented soybean meal product;
[0011] Step 3: Mix soybean meal fermentation product, ethanol solution and montmorillonite, add cysteine and cinnamaldehyde derivative, heat and stir, add eugenol and ammonium persulfate and continue stirring, centrifuge, dry and obtain prebiotic additive.
[0012] As a preferred embodiment of the present invention, in step one, the sieving is performed through a 50-mesh sieve; the stirring and mixing is performed at a speed of 300-500 r / min for 15-20 min.
[0013] As a preferred embodiment of the present invention, in step one, the mass ratio of soybean meal to deionized water is 38-40:60-65.
[0014] As a preferred embodiment of the present invention, in step two, the fermentation is carried out at a temperature of 30-40℃ for 42-50 hours; the mass ratio of the soybean meal base liquid to the mixed microbial agent is 30-35:20-22.
[0015] As a preferred embodiment of the present invention, in step two, the concentration of Bacillus subtilis in the mixed bacterial agent is 10. 7 -10 8 CFU / g and the concentration of Saccharomyces cerevisiae was 10. 6 -10 7 CFU / g; the mixed bacterial agent also includes the following components by weight: 6-8 parts glucose, 0.2-0.4 parts dipotassium hydrogen phosphate, 1.5-1.8 parts potassium sorbate, 2.0-2.3 parts fructose and 60-64 parts deionized water;
[0016] As a preferred embodiment of the present invention, in step three, the stirring and mixing is carried out at a speed of 500-600 rpm for 20-30 minutes; the heating and stirring is carried out at a temperature of 60-70℃ for 6-7 hours; and the continued stirring is carried out at a temperature of 65-70℃ for 4-5 hours.
[0017] As a preferred embodiment of the present invention, in step three, the mass ratio of the soybean meal fermentation product, ethanol solution, montmorillonite, cysteine, cinnamaldehyde derivative, eugenol, and ammonium persulfate is 8-10:120-130:20-22:2.8-3.2:2.6-3.0:2.2-2.4:0.10-0.12; the concentration of the ethanol solution is 60 wt%; and the cinnamaldehyde derivative is 3-methoxy-4-hydroxycinnamaldehyde.
[0018] A method for preparing a feed rich in prebiotic and probiotic complex as described above, the method comprising the following steps: mixing the nutritional additives and the base feed to obtain the feed.
[0019] The beneficial effects of this invention are:
[0020] This invention utilizes a combination of Bacillus subtilis and Saccharomyces cerevisiae to ferment soybean meal, effectively increasing the content of polypeptides and other prebiotics in the fermentation products, thereby significantly improving the nutritional value of the nutritional additive. By using montmorillonite as a carrier, the fermentation products of the soybean meal can adhere to montmorillonite, followed by chemical grafting of cysteine, cinnamaldehyde derivatives, and eugenol, which can significantly improve the stability of the nutritional additive and its ABTS+ free radical scavenging ability. Detailed Implementation
[0021] To further illustrate the technical means and effects adopted by the present invention to achieve its intended purpose, the following detailed description, in conjunction with embodiments, details the specific implementation methods, structures, features, and effects of the present invention. The basic feed used in the following embodiments and comparative examples can vary depending on the species being fed; for example, the basic feed required for poultry, livestock, and commercial animals will be adjusted accordingly. Further example: The basic feed composition used in the following embodiments and comparative examples is as follows (wt%): wheat bran 4.6%, soybean meal 23.8%, fish meal 2.05%, rapeseed meal 4.1%, dicalcium phosphate 1.35%, salt 0.27%, vitamins 0.04% (a mixture of equal proportions of vitamins B1, B2, B6, and C), and the remainder corn. The viable cell concentrations in the Bacillus licheniformis freeze-dried powder, Bacillus subtilis freeze-dried powder, Lactobacillus acidophilus freeze-dried powder, and Saccharomyces cerevisiae freeze-dried powder are all approximately 5.5 × 10⁻⁶. 11 per g.
[0022] Example 1
[0023] A feed rich in prebiotic and probiotic complex includes a nutritional additive and a basic feed, wherein the mass ratio of the nutritional additive to the basic feed is 0.3:100, and the nutritional additive is composed of probiotics and prebiotic additives mixed at a mass ratio of 2:30; the probiotics are composed of Bacillus licheniformis freeze-dried powder, Bacillus subtilis freeze-dried powder, Lactobacillus acidophilus freeze-dried powder and Saccharomyces cerevisiae freeze-dried powder at a mass ratio of 1:1:0.6:3.
[0024] The preparation method of the prebiotic additive includes the following steps:
[0025] Step 1: Crush, grind, sieve, and sterilize the soybean meal. Then, add deionized water and stir to obtain a soybean meal base liquid. The sieving is done through a 50-mesh sieve. The stirring is performed at 300 rpm for 15 minutes. The mass ratio of soybean meal to deionized water is 38:60.
[0026] Step 2: Mix the soybean meal base liquid and the mixed microbial agent, ferment, sterilize with ultraviolet light, and dry to obtain fermented soybean meal; wherein the fermentation is carried out at 32℃ for 42 hours; the mass ratio of the soybean meal base liquid to the mixed microbial agent is 30:20; the concentration of Bacillus subtilis in the mixed microbial agent is 10. 7 CFU / g and the concentration of Saccharomyces cerevisiae was 10. 6 CFU / g; the mixed bacterial agent also includes the following components by weight: 6 parts glucose, 0.2 parts dipotassium hydrogen phosphate, 1.5 parts potassium sorbate, 2.0 parts fructose, and 60 parts deionized water;
[0027] Step 3: After mixing soybean meal fermentation product, ethanol solution, and montmorillonite, add cysteine and cinnamaldehyde derivative, heat and stir, then add eugenol and ammonium persulfate and continue stirring. After centrifugation and drying, obtain the prebiotic additive. The stirring and mixing are performed at 500 rpm for 20 minutes; the heating and stirring are performed at 60°C for 6 hours; and the continued stirring is performed at 65°C for 4 hours. The mass ratio of soybean meal fermentation product, ethanol solution, montmorillonite, cysteine, cinnamaldehyde derivative, eugenol, and ammonium persulfate is 8:120:20:2.8:2.6:2.2:0.10; the concentration of the ethanol solution is 60 wt%; and the cinnamaldehyde derivative is 3-methoxy-4-hydroxycinnamaldehyde.
[0028] A method for preparing a feed rich in prebiotic and probiotic complex includes the following steps: mixing the nutritional additives and the base feed together to obtain the final product.
[0029] Example 2
[0030] A feed rich in prebiotic and probiotic complex includes a nutritional additive and a basal feed, wherein the mass ratio of the nutritional additive to the basal feed is 0.33:100, and the nutritional additive is composed of probiotics and prebiotic additives mixed at a mass ratio of 2.3:32; the probiotics are composed of Bacillus licheniformis freeze-dried powder, Bacillus subtilis freeze-dried powder, Lactobacillus acidophilus freeze-dried powder and Saccharomyces cerevisiae freeze-dried powder at a mass ratio of 1.3:1.3:0.67:3.3.
[0031] The preparation method of the prebiotic additive includes the following steps:
[0032] Step 1: Crush, grind, sieve, and sterilize the soybean meal. Then, add deionized water and stir to obtain a soybean meal base liquid. The sieving process involves passing the soybean meal through a 50-mesh sieve. The stirring and mixing process involves stirring at 360 rpm for 17 minutes. The mass ratio of soybean meal to deionized water is 38.7:62.
[0033] Step 2: Mix the soybean meal base liquid and the mixed microbial agent evenly, ferment, sterilize with ultraviolet light, and dry to obtain fermented soybean meal; wherein the fermentation is carried out at 32℃ for 45 hours; the mass ratio of the soybean meal base liquid to the mixed microbial agent is 32:20.7; the concentration of Bacillus subtilis in the mixed microbial agent is 5*10. 7 CFU / g and the concentration of Saccharomyces cerevisiae is 5*10. 6 CFU / g; the mixed bacterial agent also includes the following components by weight: 6.7 parts glucose, 0.27 parts dipotassium hydrogen phosphate, 1.6 parts potassium sorbate, 2.1 parts fructose and 61 parts deionized water;
[0034] Step 3: After mixing soybean meal fermentation product, ethanol solution, and montmorillonite, add cysteine and cinnamaldehyde derivative, heat and stir, then add eugenol and ammonium persulfate and continue stirring. After centrifugation and drying, obtain the prebiotic additive. The stirring and mixing are performed at 530 rpm for 25 min; the heating and stirring are performed at 63℃ for 6.5 h; and the continued stirring is performed at 67℃ for 4.5 h. The mass ratio of soybean meal fermentation product, ethanol solution, montmorillonite, cysteine, cinnamaldehyde derivative, eugenol, and ammonium persulfate is 8.7:123:20.7:2.9:2.7:2.27:0.115; the concentration of the ethanol solution is 60 wt%; and the cinnamaldehyde derivative is 3-methoxy-4-hydroxycinnamaldehyde.
[0035] A method for preparing a feed rich in prebiotic and probiotic complex includes the following steps: mixing the nutritional additives and the base feed together to obtain the final product.
[0036] Example 3
[0037] A feed rich in prebiotic and probiotic complex includes a nutritional additive and a basal feed, wherein the mass ratio of the nutritional additive to the basal feed is 0.37:100, and the nutritional additive is composed of probiotics and prebiotic additives mixed at a mass ratio of 2.7:33; the probiotics are composed of Bacillus licheniformis freeze-dried powder, Bacillus subtilis freeze-dried powder, Lactobacillus acidophilus freeze-dried powder and Saccharomyces cerevisiae freeze-dried powder at a mass ratio of 1.7:1.7:0.73:3.7.
[0038] The preparation method of the prebiotic additive includes the following steps:
[0039] Step 1: Crush, grind, sieve, and sterilize the soybean meal. Then, add deionized water and stir to obtain a soybean meal base liquid. The sieving is done through a 50-mesh sieve. The stirring is performed at 430 rpm for 18 minutes. The mass ratio of soybean meal to deionized water is 39.3:63.
[0040] Step 2: Mix the soybean meal base liquid and the mixed microbial agent, ferment, sterilize with ultraviolet light, and dry to obtain fermented soybean meal; wherein the fermentation is carried out at 32℃ for 47 hours; the mass ratio of the soybean meal base liquid to the mixed microbial agent is 33:21.3; the concentration of Bacillus subtilis in the mixed microbial agent is 5*10. 7 CFU / g and the concentration of Saccharomyces cerevisiae is 5*10. 6 CFU / g; the mixed bacterial agent also includes the following components by weight: 7.3 parts glucose, 0.33 parts dipotassium hydrogen phosphate, 1.7 parts potassium sorbate, 2.2 parts fructose and 63 parts deionized water;
[0041] Step 3: After mixing soybean meal fermentation product, ethanol solution, and montmorillonite, add cysteine and cinnamaldehyde derivative, heat and stir, then add eugenol and ammonium persulfate and continue stirring. After centrifugation and drying, obtain the prebiotic additive. The stirring and mixing are performed at 560 rpm for 25 min; the heating and stirring are performed at 67℃ for 6.5 h; and the continued stirring is performed at 68℃ for 4.5 h. The mass ratio of soybean meal fermentation product, ethanol solution, montmorillonite, cysteine, cinnamaldehyde derivative, eugenol, and ammonium persulfate is 9.3:127:21.3:3.1:2.9:2.33:0.115; the concentration of the ethanol solution is 60 wt%; and the cinnamaldehyde derivative is 3-methoxy-4-hydroxycinnamaldehyde.
[0042] A method for preparing a feed rich in prebiotic and probiotic complex includes the following steps: mixing the nutritional additives and the base feed together to obtain the final product.
[0043] Example 4
[0044] A feed rich in prebiotic and probiotic complex includes a nutritional additive and a basic feed, wherein the mass ratio of the nutritional additive to the basic feed is 0.4:100, and the nutritional additive is composed of probiotics and prebiotic additives mixed at a mass ratio of 3:35; the probiotics are composed of Bacillus licheniformis freeze-dried powder, Bacillus subtilis freeze-dried powder, Lactobacillus acidophilus freeze-dried powder and Saccharomyces cerevisiae freeze-dried powder at a mass ratio of 2:2:0.8:4.
[0045] The preparation method of the prebiotic additive includes the following steps:
[0046] Step 1: Crush, grind, sieve, and sterilize the soybean meal. Then, add deionized water and stir to obtain a soybean meal base liquid. The sieving is done through a 50-mesh sieve. The stirring is performed at 500 rpm for 20 minutes. The mass ratio of soybean meal to deionized water is 40:65.
[0047] Step 2: Mix the soybean meal base liquid and the mixed microbial agent, ferment, sterilize with ultraviolet light, and dry to obtain fermented soybean meal; wherein the fermentation is carried out at 32℃ for 50 hours; the mass ratio of the soybean meal base liquid to the mixed microbial agent is 35:22; the concentration of Bacillus subtilis in the mixed microbial agent is 10. 8 CFU / g and the concentration of Saccharomyces cerevisiae was 10. 7 CFU / g; the mixed bacterial agent also includes the following components by weight: 8 parts glucose, 0.4 parts dipotassium hydrogen phosphate, 1.8 parts potassium sorbate, 2.3 parts fructose, and 64 parts deionized water;
[0048] Step 3: After mixing soybean meal fermentation product, ethanol solution, and montmorillonite, add cysteine and cinnamaldehyde derivative, heat and stir, then add eugenol and ammonium persulfate and continue stirring. After centrifugation and drying, obtain the prebiotic additive. The stirring and mixing are performed at 600 rpm for 30 minutes; the heating and stirring are performed at 70°C for 7 hours; and the continued stirring is performed at 70°C for 5 hours. The mass ratio of soybean meal fermentation product, ethanol solution, montmorillonite, cysteine, cinnamaldehyde derivative, eugenol, and ammonium persulfate is 10:130:22:3.2:3.0:2.4:0.12; the concentration of the ethanol solution is 60 wt%; and the cinnamaldehyde derivative is 3-methoxy-4-hydroxycinnamaldehyde.
[0049] A method for preparing a feed rich in prebiotic and probiotic complex includes the following steps: mixing the nutritional additives and the base feed together to obtain the final product.
[0050] Comparative Example 1
[0051] Compared with Example 3, Comparative Example 1 differs in that montmorillonite is not used, while the other components, preparation steps and parameters are the same.
[0052] Comparative Example 2
[0053] Compared with Example 3, Comparative Example 2 differs in that cysteine is not used, while the other components, preparation steps and parameters are the same.
[0054] Comparative Example 3
[0055] Compared with Example 3, Comparative Example 3 differs in that cinnamaldehyde is used instead of cinnamaldehyde derivative, while the other components, preparation steps and parameters are the same.
[0056] Comparative Example 4
[0057] Compared with Example 3, Comparative Example 4 differs in that it does not use cinnamaldehyde derivatives, while the other components, preparation steps and parameters are the same.
[0058] Comparative Example 5
[0059] Compared with Example 3, Comparative Example 5 differs in that eugenol is not used, while the other components, preparation steps and parameters are the same.
[0060] The prebiotic additives prepared in Examples 1-4 and Comparative Examples 1-5 were tested for the following properties, and the test results are shown in Table 1.
[0061] ABTS + Free radical scavenging rate = (A 空白 -A 样品 ) / A 空白 *100%
[0062] Table 1: Test Results
[0063] ABTS+ free radical scavenging rate (%) ABTS+ free radical scavenging rate (%) after treatment at 50℃ for 24 h Example 1 92.8 91.2 Example 2 93.3 91.6 Example 3 93.6 92.1 Example 4 93.4 91.6 Comparative Example 1 75.7 69.4 Comparative Example 2 78.6 73.2 Comparative Example 3 84.7 79.8 Comparative Example 4 77.8 71.6 Comparative Example 5 86.2 81.3
[0064] As can be seen from the test results in Table 1, compared with Comparative Examples 1-5, the prebiotic additives prepared by the present invention have excellent stability and ABTS+ free radical scavenging ability.
[0065] Comparative analysis reveals that this invention, through the combined fermentation of soybean meal with Bacillus subtilis and Saccharomyces cerevisiae, effectively increases the content of polypeptides and other prebiotics in the fermentation products, thereby significantly improving the nutritional value of the nutritional additive. Utilizing montmorillonite as a carrier, the soybean meal fermentation products can adhere to montmorillonite, increasing its stability. The sulfhydryl groups of cysteine can form coordinate bonds with the metal ions of montmorillonite, promoting cysteine adhesion. Subsequently, cysteine chemically grafts with cinnamaldehyde derivatives, coating the soybean meal fermentation products and montmorillonite, introducing a Schiff base structure with strong antioxidant capacity and high stability. Compared to cinnamaldehyde, the oxygen-containing functional groups on the cinnamaldehyde derivative possess higher antioxidant capacity. Combined with its own highly stable benzene ring structure, it can significantly improve the stability and antioxidant capacity of the prebiotic additive. The double bonds on the cinnamaldehyde derivative can graft with eugenol, further enhancing the stability and ABTS+ free radical scavenging ability of the prebiotic additive.
[0066] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any indirect modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.
Claims
1. A feed rich in prebiotic and probiotic complex, comprising a nutritional additive and a basal feed, wherein the mass ratio of the nutritional additive to the basal feed is 0.3-0.4:100, characterized in that: The nutritional additive is composed of probiotics and prebiotic additives mixed in a mass ratio of 2-3:30-35. The probiotics consist of Bacillus licheniformis freeze-dried powder, Bacillus subtilis freeze-dried powder, Lactobacillus acidophilus freeze-dried powder, and Saccharomyces cerevisiae freeze-dried powder. The preparation method of the prebiotic additive includes the following steps: Step 1: Crush, grind, sieve, sterilize, add deionized water and stir to mix to obtain soybean meal base liquid; Step 2: Mix the soybean meal base liquid and the mixed microbial agent, ferment, sterilize with ultraviolet light, and dry to obtain the fermented soybean meal product; Step 3: Mix soybean meal fermentation product, ethanol solution and montmorillonite, add cysteine and 3-methoxy-4-hydroxycinnamaldehyde, heat and stir, add eugenol and ammonium persulfate and continue stirring, centrifuge, dry and obtain prebiotic additive; In step three, the mass ratio of the fermented soybean meal, ethanol solution, montmorillonite, cysteine, 3-methoxy-4-hydroxycinnamaldehyde, eugenol, and ammonium persulfate is 8-10:120-130:20-22:2.8-3.2:2.6-3.0:2.2-2.4:0.10-0.12; the concentration of the ethanol solution is 60 wt%. In step three, the heating and stirring is carried out at a temperature of 60-67℃ for 6-7 hours; the continued stirring is carried out at a temperature of 65-70℃ for 4-5 hours. The probiotics are composed of Bacillus licheniformis freeze-dried powder, Bacillus subtilis freeze-dried powder, Lactobacillus acidophilus freeze-dried powder and Saccharomyces cerevisiae freeze-dried powder in a mass ratio of 1-2:1-2:0.6-0.8:3-4.
2. The feed rich in prebiotic and probiotic complex according to claim 1, characterized in that: In step one, the sieving is done through a 50-mesh sieve; the stirring and mixing is done at a speed of 300-500 r / min for 15-20 min.
3. The feed rich in prebiotic and probiotic complex according to claim 1, characterized in that: In step one, the mass ratio of soybean meal to deionized water is 38-40:60-65.
4. The feed rich in prebiotic and probiotic complex according to claim 1, characterized in that: In step two, the fermentation is carried out at a temperature of 30-40℃ for 42-50 hours; the mass ratio of the soybean meal base liquid to the mixed microbial agent is 30-35:20-22.
5. The feed rich in prebiotic and probiotic complex according to claim 1, characterized in that: In step two, the concentration of Bacillus subtilis in the mixed bacterial agent is 10. 7 -10 8 CFU / g and the concentration of Saccharomyces cerevisiae was 10. 6 -10 7 CFU / g; the mixed bacterial agent also includes the following components by weight: 6-8 parts glucose, 0.2-0.4 parts dipotassium hydrogen phosphate, 1.5-1.8 parts potassium sorbate, 2.0-2.3 parts fructose and 60-64 parts deionized water.
6. The feed rich in prebiotic and probiotic complex according to claim 1, characterized in that: In step three, the stirring and mixing is carried out at a speed of 500-600 rpm for 20-30 minutes.
7. A method for preparing a feed rich in prebiotic and probiotic complex as described in any one of claims 1 to 6, characterized in that, Includes the following steps: The nutritional additives and basic feed are mixed evenly to obtain the final product.