Polypeptide quantitative analysis method integrating multiple liquid chromatography-mass spectrometry maps and application thereof

Abstract

The invention discloses a polypeptide quantitative analysis method integrating multiple liquid chromatography-mass spectrometry maps and application of the polypeptide quantitative analysis method, and belongs to the cross technical field of analytical chemistry and computer disciplinary. A sample is analyzed by using a liquid chromatography-mass spectrometry technology to obtain multiple extracted ion flow chromatograms of secondary fragment ions; a plurality of liquid chromatography-mass spectrometry maps are integrated, the original mode of sequentially processing single maps and then combining results is changed, the retention time, the ion mass-to-charge ratio and the consistency of the relative strength of the same component are analyzed for multiple times by using the liquid chromatography-mass spectrometry technology, and inconsistent chromatographic peaks and secondary fragment ions in multiple times of analysis are eliminated; the accuracy of the screened quantitative chromatographic peak and quantitative ion is improved, the relative content of the polypeptide in the samples is calculated by utilizing the screened quantitative chromatographic peak and quantitative ion, the quantitative accuracy of the polypeptide and the protein is improved, and the method can be used for proteome non-targeted analysis of clinical samples and biological samples.

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