生物修复剂及其制备方法和应用、石油污染土壤的修复方法

By using a bioremediation agent made from the fermentation liquid of fresh plant *Pseudolarix amabilis* stalks and *Arthrobacter* strains, the problems of numerous materials, complex processes, and high costs associated with oil-contaminated soil remediation agents have been solved. This has enabled efficient and low-cost remediation of oil-contaminated soil without affecting soil biosafety.

CN119839033BActive Publication Date: 2026-07-17PETROCHINA CO LTD

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
PETROCHINA CO LTD
Filing Date
2023-10-17
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing oil-contaminated soil remediation agents are made from a variety of materials, involve complex processes, and are costly. They may also cause soil microbial safety issues. Traditional methods are ineffective and time-consuming for remediating oil-contaminated soil with an oil content of more than 10%.

Method used

Using fresh plant *Pseudolarix amabilis* stalk fermentation liquid and *Arthrobacter* strain as bioremediation agents, *Pseudolarix amabilis* fermentation liquid is produced through anaerobic fermentation for the remediation of petroleum-contaminated soil. This simplifies the process and reduces costs, does not introduce exogenous microorganisms, and utilizes *Pseudolarix amabilis* to enhance soil fertility.

Benefits of technology

It achieves efficient remediation of petroleum-contaminated soil with an oil content of 10% to 20%, with a petroleum hydrocarbon degradation rate of over 88.7%. The operation is simple, low-cost, and does not affect the safety of soil biological populations, while shortening the remediation cycle.

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Abstract

本发明提供了一种生物修复剂及其制备方法和应用、石油污染土壤的修复方法,其中石油污染土壤的生物修复剂的制备方法,包括以下步骤:S1将新鲜植物假苇拂子茅杆切段,加水,厌氧发酵2~3天;S2添加接种量为1wt%~2wt%保藏编号为CGMCC No.6581的节杆菌菌株,在25~35℃的温度下发酵7~10天,制成假苇拂子茅酵液,即得生物修复剂。本发明使用新鲜植物假苇拂子茅,利用其发酵液及植物本身作为修复剂,来降解污染土壤中石油烃,该生物修复剂整体加工工艺较简单,修复过程操作便捷,减少了人为操作过程。同时采用的新鲜植物假苇拂子茅草随处可得,制作成本低,且未引入多种外源性微生物,整体修复过程中土壤生物种群安全性未受影响。
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