A novel polyester intermediate and a preparation method and application thereof

By using BHET-MHET to screen compounds and protein catalysis to acyltransferase activity, the problem of time-consuming and limited detection of polyester depolymerase activity by HPLC has been solved, achieving efficient and accurate screening and activity detection of polyester depolymerase.

CN119841732BActive Publication Date: 2026-07-03TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Filing Date
2023-10-16
Publication Date
2026-07-03

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Patent Text Reader

Abstract

The application discloses a novel polyester intermediate and a preparation method and application thereof, and the novel polyester intermediate is O,O'-(ethane-1,2-diacyl) bis(2-hydroxyethyl) diphenyldicarboxylate (BHET-MHET). Experiments prove that, by taking BHET-MHET as a substrate, OD 600nm The polyester degradation enzyme can be polyester depolymerase LCC, polyester depolymerase TfCut2 or polyester depolymerase PETase. The polyester depolymerase screened by using BHET-MHET has the characteristics of high accuracy and strong reliability. The application has important application value.
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