White wheat ear sprouting resistance gene ta myb10-94e and application thereof

Abstract

The application discloses a white wheat ear sprouting resistance gene TaMyb10-94E and application thereof. The application clones a TaMyb10-94E gene from a 3D chromosome of Suining Tuotuo wheat, and proves that overexpression of the TaMyb10-94E gene does not cause red grain color of wheat as overexpression of other TaMyb10 genes, and simultaneously has the function of enhancing grain dormancy and reducing grain sprouting rate. It is shown that the special variation of the TaMyb10-94E gene from the Suining Tuotuo wheat can impart the wheat ear sprouting resistance without producing red grains. The application has important application value for cultivating white grain and ear sprouting resistant wheat.

Description

Technical Field

[0001] This invention belongs to the field of biotechnology, specifically relating to a gene for resistance to germination in white-grained wheat ears. TaMyb10-94E And its applications. Background Technology

[0002] Wheat is a vital global food crop, with approximately 35%–40% of the world's population relying on it as their primary food source. Sprouting on the ear refers to the phenomenon where wheat seeds germinate on the ear due to prolonged periods of damp weather before harvest. Sprouting on the ear leads to a rapid increase in the activity of related hydrolytic enzymes in the wheat grains, degrading storage substances and severely impacting wheat quality. It also affects storage and the germination rate of the following year's sowing, causing significant economic losses to wheat production. Long-term production practices have revealed a close relationship between wheat seed coat color and sprouting on the ear. Common wheat seed coat colors are mainly red and white, with red generally exhibiting stronger resistance to sprouting on the ear than white. In the rainy wheat-growing regions of Sichuan, Anhui, and Jiangsu, where sprouting on the ear is severe, red-skinned wheat is commonly planted. However, white-skinned wheat is more popular in production due to its thinner skin, relatively higher endosperm content, and higher flour yield. Therefore, the discovery and utilization of sprouting resistance genes in white-grained wheat is of significant value. Summary of the Invention

[0003] The purpose of this invention is to provide a gene for resistance to germination in white-grained wheat ears. TaMyb10-94E And its applications.

[0004] wheat TaMyb10 This gene activates the flavonoid and ABA hormone synthesis pathways, simultaneously regulating wheat grain color and germination rate. Wheat varieties possessing this gene on chromosome 3D mostly have red grains. Suining Tuotuo wheat, a white-grained wheat variety, has a germination index of 10.1%, classifying it as a resistant to pre-harvest germination. In the F2 generation of a Suining Tuotuo wheat (white grain) × Chinese Spring (red grain) hybrid population, individuals with extremely red and white grains were selected for mixed sequencing to observe the relationship between grain color and germination rate. TaMyb10 Co-segregation of gene allelic variations revealed that all mixed samples of white-grained wheat materials were... TaMyb10-94E The genetic type of the red-grained wheat materials showed heterozygosity in the mixed sample, indicating that... TaMyb10-94E The gene does not cause wheat grains to turn red. Therefore, the 3D chromosome from Suining Tuotuo wheat... TaMyb10-94E Special genetic variations can confer wheat ear germination resistance without producing red grains.

[0005] To achieve the objectives of this invention, in a first aspect, this invention provides a white-grained wheat spike germination resistance gene. TaMyb10- 94E (From Tuotuo wheat from Suining, Sichuan), it is a gene encoding either protein (a) or (b) as follows: (a) A protein consisting of the amino acid sequence shown in SEQ ID NO:1; (b) A protein derived from (a) with the sequence shown in SEQ ID NO:1 substituted, deleted or added with one or more amino acids and having the same function.

[0006] Gene TaMyb10-94E The nucleotide sequence is as follows: i) The nucleotide sequence shown in SEQ ID NO:2; ii) A nucleotide sequence of the nucleotide sequence shown in SEQ ID NO:2 that has been substituted, deleted and / or added with one or more nucleotides and expresses a protein with the same function; iii) A nucleotide sequence that hybridizes with the sequence shown in SEQ ID NO:2 under stringent conditions and expresses a protein with the same function, wherein the stringent conditions are hybridization at 65°C in 0.1×SSPE containing 0.1% SDS or 0.1×SSC containing 0.1% SDS, followed by washing the membrane with the same solution; or iv) Nucleotide sequences that have more than 90% homology with the nucleotide sequences of i), ii) or iii) and express the same functional protein.

[0007] Secondly, the present invention provides a product containing the aforementioned gene. TaMyb10-94E Biological materials, including but not limited to recombinant DNA, expression cassettes, transposons, plasmid vectors, viral vectors, engineered bacteria, or transgenic cell lines.

[0008] Thirdly, the present invention provides the aforementioned gene. TaMyb10-94E Or any of the following applications of biological materials containing the said gene: (1) Used to regulate the color of wheat grains; (2) Used to regulate the germination rate of wheat grains; (3) Used for plant breeding or improvement of plant germplasm resources; (4) Used to prepare transgenic plants.

[0009] Furthermore, the application includes: 1) Make wheat contain genes TaMyb10-94E ;or 2) Enable wheat to express genes TaMyb10-94E Encoded protein; or 3) Cause wheat to overexpress genes TaMyb10-94E ; This allows for the production of white-grained wheat resistant to ear sprouting.

[0010] Fourthly, the present invention provides a method for constructing white-grained wheat resistant to ear sprouting, the method comprising: using genetic engineering techniques to overexpress the gene in wheat. TaMyb10-94E .

[0011] Furthermore, the overexpression method can be selected from the following 1) to 6), or any combination thereof: 1) By importing a plasmid containing the gene; 2) By increasing the copy number of the aforementioned genes on plant chromosomes; 3) By altering the promoter sequence of the aforementioned genes on plant chromosomes; 4) By operatively linking a strong promoter to the gene; 5) By introducing enhancers; 6) Using gene editing techniques to remove certain components from wheat. TaMyb10 Genetically modified TaMyb10-94E Gene.

[0012] Expression vectors carrying the target gene can be introduced into plant cells using conventional biotechnological methods such as Ti plasmids, plant virus vectors, direct DNA transformation, microinjection, and electroporation (Weissbach, 1998, Method for Plant Molecular Biology VIII, Academy Press, New York, pp. 411-463; Geiserson and Corey, 1998, Plant Molecular Biology, 2). nd Edition).

[0013] Preferably, the expression vector containing the gene is transformed into Agrobacterium, and the gene is transferred into the plant through Agrobacterium-mediated transformation, so that the gene is overexpressed in the plant.

[0014] Fifthly, the present invention provides the application of transgenic wheat obtained according to the method in plant breeding.

[0015] Furthermore, breeding methods include, but are not limited to, transgenic, hybridization, backcrossing, self-pollination, or asexual reproduction.

[0016] By employing the above technical solution, the present invention has at least the following advantages and beneficial effects: This invention is the first to identify a 3D chromosome-derived gene from Suining Tuotuo wheat. TaMyb10-94E Gene. Compared with other wheat TaMYB10 proteins, including Chinese Spring wheat, the 94th amino acid of the TaMYB10-94E protein in Tuotuo wheat changes from glycine (G) to glutamic acid (E). Experiments demonstrate that this invention discovered... TaMyb10-94EThe gene and its encoded protein can control wheat grain color and pre-sprouting resistance, and its function has been further verified, making it suitable for constructing white-grained wheat resistant to pre-sprouting. This gene can be used in plant genetic engineering, gene editing technology, and molecular breeding. Attached Figure Description

[0017] Figure 1 Suining Tuotuo Wheat is a preferred embodiment of the present invention. TaMyb10-94E Other wheat TaMyb10 Comparison of nucleic acid sequences of genes.

[0018] Figure 2 In a preferred embodiment of the present invention, the TaMyb10-94E protein sequence of Tuotuo wheat is compared with the TaMyb10 protein sequence of other wheat varieties, represented by Chinese Spring wheat (protein-mc is Chinese Spring wheat, protein-mt is Suining Tuotuo wheat).

[0019] Figure 3 In a preferred embodiment of the present invention TaMyb10 Gene expression in Tuotuo wheat seeds.

[0020] Figure 4 In a preferred embodiment of the present invention, the grain color of the hybrid offspring of Suining Tuotuo wheat (white grain) × Chinese Spring wheat (red grain) is similar to... TaMyb10 Identification of co-segregation of gene allelic variations.

[0021] Figure 5 In a preferred embodiment of the present invention TaMyb10-94E Identification results of overexpression of transgenic wheat.

[0022] Figure 6 In a preferred embodiment of the present invention TaMyb10-94E - OE Germination rate of genetically modified wheat grains.

[0023] Figure 7 In a preferred embodiment of the present invention TaMyb10-94E - OE Genetically modified wheat (No. 2 and No. 12) and TaMyb10-OE Comparison of grain color of genetically modified wheat (58-4#). Detailed Implementation

[0024] This invention provides a germination resistance gene for white-grained wheat (Suining Tuotuo wheat) in the ear. TaMyb10-94E and its cloning methods, including TaMyb10-94E Gene extraction, cloning, and functional analysis. This gene, derived from Suining Tuotuo wheat, is responsible for resistance to germination in white-grained wheat ears. TaMyb10-94EHaving a specific base sequence, its protein is named TaMYB10-94E. It has a different amino acid sequence compared to known wheat TaMYB10 proteins and possesses characteristics not found in other TaMYB10 proteins (it does not produce the red grain trait). It exhibits a different type of function and is a unique type not yet discovered in the relevant field. Specifically, it is as follows (a) or (b): (a) A protein consisting of the amino acid sequence shown in SEQ ID NO:1; (b) A protein derived from SEQ ID NO:1 by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence of SEQ ID NO:1 and which is associated with plant grain color and ear germination.

[0025] To facilitate the purification and detection of the protein in (a), a label as shown in Table 1 can be attached to the amino or carboxyl terminus of the protein shown in (a).

[0026] Table 1 Tag Sequence Poly-Arg 5-6 (usually 5) RRRRR Poly-His 2-10 (usually 6) HHHHHH FLAG 8 DYKDDDDK Strep-tag II 8 WSHPQFEK c-myc 10 EQKLISEEDL The protein in (b) above can be synthesized artificially, or its encoding gene can be synthesized first and then expressed biologically. The encoding gene of the protein in (b) above can be obtained by deleting one or more amino acid residues from the codon of the encoding gene of TaMYB10-94E protein, and / or by performing a missense mutation of one or more base pairs, and / or by attaching the coding sequence of the tag shown in Table 1 to its 5' end and / or 3' end.

[0027] The gene encoding the TaMYB10-94E protein ( TaMyb10-94E Genes are also within the scope of protection of this invention.

[0028] The TaMyb10-94E The gene is any one of the DNA molecules described in (1)-(3) below: (1) A DNA molecule with a coding region as shown in SEQ ID NO:2; (2) A DNA molecule that hybridizes to the DNA sequence defined in (1) under strict conditions and encodes a protein related to the color of plant seeds; (3) A DNA molecule that has more than 90% homology with the DNA sequence defined in (1) or (2) and encodes a protein related to the color of plant seeds.

[0029] The above stringent conditions can be achieved by hybridization at 65°C and washing the membrane in a solution of 0.1×SSPE (or 0.1×SSC) and 0.1% SDS during DNA or RNA hybridization experiments.

[0030] Those skilled in the art can use known methods, such as directed evolution and point mutation, to mutate the nucleotide sequence encoding the TaMYB10-94E protein of the present invention. Any artificially modified nucleotides that have 75% or higher identity with the nucleotide sequence of the TaMYB10-94E protein isolated according to the present invention, as long as they encode the TaMYB10-94E protein, are derived from and equivalent to the nucleotide sequence of the present invention.

[0031] The present invention also provides a product containing the aforementioned TaMyb10-94E Recombinant gene expression vectors, expression cassettes, transgenic cell lines, or recombinant bacteria.

[0032] This invention also protects the use of any of the above-described TaMYB10-94E proteins, or any of the above-described nucleic acid molecules, or expression cassettes, recombinant vectors, or recombinant microorganisms containing any of the above-described nucleic acid molecules in regulating wheat grain color and ear sprouting resistance, as well as in the use in breeding transgenic wheat with white grains resistant to ear sprouting.

[0033] Existing plant expression vectors can be used to construct structures containing... TaMyb10-94E Recombinant gene expression vectors. These plant expression vectors include binary Agrobacterium vectors and vectors suitable for plant micro-bombardment. Use TaMyb10-94E When constructing recombinant expression vectors, any type of enhancing, constitutive, tissue-specific, or inducible promoter can be added before the transcription initiation nucleotide. These can be used alone or in combination with other plant promoters. Furthermore, using… TaMyb10-94E When constructing recombinant expression vectors, enhancers, including translational enhancers or transcriptional enhancers, can be used. These enhancer regions can be ATG start codons or adjacent start codons, but they must be identical to the reading frame of the coding sequence to ensure correct translation of the entire sequence. The sources of the translation control signals and start codons are wide-ranging; they can be natural or synthetic. The translation initiation region can originate from the transcription initiation region or structural genes. To facilitate the identification and screening of transgenic plant cells or plants, the plant expression vectors used can be processed, such as by adding genes that express enzymes or luminescent compounds that produce color changes in plants, antibiotic resistance markers, or chemical reagent resistance marker genes.

[0034] The recombinant expression vector may specifically be a vector that... pUBI:cas The fragment between the BamHI and PmlI restriction sites of the vector was replaced with the DNA molecule shown in SEQ ID NO:2 to obtain the recombinant expression vector.

[0035] The following examples are for illustrative purposes only and are not intended to limit the scope of the invention. Unless otherwise specified, the examples are conducted under conventional experimental conditions, such as those described in Sambrook et al., Molecular Cloning: a Laboratory Manual (Sambrook J & Russell DW, 2001), or as recommended in the manufacturer's instructions.

[0036] In the transcriptome sequencing examples below, all experiments were performed in triplicate, and the average value of the results was taken.

[0037] For the local wheat variety Tuotuo Wheat from Suining, Sichuan, please refer to Zhou Yong. Evaluation of Sprouting Resistance and Genome-Wide Association Analysis of Local Wheat Varieties in China [D]. Sichuan Agricultural University, 2017; the public can obtain this biological material from the Wheat Research Institute of Sichuan Agricultural University.

[0038] Other wheat TaMyb10 The transgenic line 58-4# overexpressing the gene (sequence shown in SEQ ID NO:3) can be found in Lang J, Fu Y, Zhou Y, Cheng M, Deng M, Li M, Zhu T, Yang J, Guo X, Gui L, LiL, Chen Z, Yi Y, Zhang L, Hao M, Huang L, Tan C, Chen G, Jiang Q, Qi P, Pu Z, Ma J, Liu Z, Liu Y, Luo MC, Wei Y, Zheng Y, Wu Y, Liu D, Wang J. Myb10-Dconfers PHS-3D resistance to pre-harvest sprouting by regulating NCED in ABAbiosynthesis pathway of wheat [J]. New phytologist, 2021, 230(5): 1940-52. This biomaterial can be obtained from the Wheat Research Institute of Sichuan Agricultural University.

[0039] pUBI-CAMBIA3301For the vector, see Zhang L, He G, Li Y, et al. PILtranscription factors directly interact with SPLs and repress tillering / branching in plants[J]. New Phytologist, 2022, 233(3): 1414-1425. The public can obtain this biomaterial from the Wheat Research Institute of Sichuan Agricultural University.

[0040] Example 1: Obtaining TaMYB10-94E protein and its encoding gene Total RNA was extracted from the common wheat variety Suining Tuotuo wheat and reverse transcribed into cDNA. PCR amplification was performed using primer pairs consisting of TaMYB10-F: 5'-ATGGGGAGGAAGCCATGCTGCGCCA-3' and TaMYB10-R: 5'-CTAGCAAAGCCACGCCAACTCCAGG-3'. The reaction program was: 94℃ for 2 min; 94℃ for 30 sec, 60℃ for 30 sec, 68℃ for 1 min, 34 cycles; 68℃ for 10 min. Approximately 798 bp of the PCR amplification product was recovered and sent to Qingke Biotechnology Co., Ltd. for sequencing. Sequencing results showed that the DNA sequence shown in SEQ ID NO:2 was obtained from the cDNA amplification product, and the protein encoded by it is shown in SEQ ID NO:1.

[0041] The protein shown in SEQ ID NO:1 is named TaMYB10-94E protein. The gene encoding TaMYB10-94E protein is named... TaMyb10-94E The gene, whose open reading frame is shown in SEQ ID NO:2.

[0042] Compared with other wheat varieties, including Chinese spring wheat TaMYB10 Gene comparison, Tuotuo wheat TaMyb10-94E The 281st base in the gene coding region changes from G to A ( Figure 1 ).

[0043] Compared with other wheat TaMYB10 proteins, including Chinese Spring wheat, the 94th amino acid in the TaMYB10-94E protein of Tuotuo wheat changes from glycine (G) to glutamic acid (E). Figure 2 ).

[0044] Example 2 TaMyb10-94E Genes and seed color co-segregate 1. Red-grained wheat, Chinese Spring, was crossed with white-grained wheat, Suining Tuotuo Wheat. The resulting seeds were designated F0 and propagated through field self-pollination to the F3 generation.

[0045] 2. Leaves were collected from each F3 wheat plant and tagged. Total DNA was extracted using the CTAB method and used as a template. PCR amplification was performed using the primer pair TaMYB10-F and TaMYB10-R described above. Approximately 798 bp of the PCR amplification product was recovered and sent to Qingke Biotechnology Co., Ltd. for sequencing.

[0046] 3. Harvest the F3 generation, and treat each wheat grain with vanillin reagent. Vanillin reacts with resorcinol or phloroglucinol, monomers of proanthocyanidins and catechins in the grain, to form a red carbocation under concentrated acid, which causes the grain to exhibit different colors. Analysis of the sequencing results from step 2 indicates that red-grained wheat in both the Chinese spring and F3 generations is... TaMYB10 Genotypes: Suining Tuotuo wheat (TTM) and F3 generation medium white wheat are both TaMyb10-94E genotype( Figure 4 ),illustrate TaMyb10-94E Genes co-segregated with white seeds.

[0047] Example 3 TaMyb10-94E Gene expression pattern analysis 1. Suining Tuotuo wheat was cultivated in an artificial climate chamber for 3 months, and samples were taken at different times after flowering (5d, 7d, 10d, 12d, 15d, 20d).

[0048] 2. Extract total RNA from samples obtained at each time point in step 1 and send them to Beijing Berry Genomics Co., Ltd. (berrygenomics.com) for next-generation transcriptome sequencing. The total data volume for a single sample is 6G. Kallisto was used to calculate the TPM of the genes, yielding the results for Tuotuo wheat. TaMyb10-94E The expression trend. The results are as follows: Figure 3 As shown. The results indicate that, TaMyb10-94E The expression level peaked on day 5 and day 10, and dropped to 0 on day 20.

[0049] Example 4 TaMyb10-94E Application of genes in regulating wheat grain color and ear germination resistance 1. Construction of recombinant expression vectors The fragment between the BamHI and PmlI restriction sites in the pUBI-CAMBIA3301 vector was replaced with the DNA molecule shown in SEQ ID NO:2 to obtain the recombinant expression vector (which has been verified by sequencing).

[0050] 2. Obtaining recombinant Agrobacterium The recombinant expression vector obtained in step 1 was introduced into Agrobacterium tumefaciens EHA105 using the heat shock conversion method to obtain recombinant Agrobacterium.

[0051] 3. Acquisition and Identification of Genetically Modified Wheat Using Agrobacterium-mediated genetic transformation, the recombinant Agrobacterium obtained in step 2 was transformed into the immature embryos of the wheat variety Fielder (white-grained, non-resistant to pre-budding ears), resulting in transgenic wheat plants. TaMyb10-94E-OE The Fielder genome does not contain TaMyb10-94E Therefore, DNA was extracted from leaves of control Fielder (WT) and transgenic wheat, and PCR amplification was performed using the primer pair TamyB10-F and TamyB10-R described above. The results are as follows: Figure 5 As shown, transgenic lines can amplify [the following]. TaMyb10-94E No amplified band was observed in the gene in WT.

[0052] 4. Phenotypic analysis of transgenic plants Plants to be tested: WT, obtained in step 3 TaMyb10-94E-OE Transgenic lines (2# and 12#) and overexpression of other wheat strains under the Fielder background TaMyb10 The transgenic line 58-4# was obtained from the gene.

[0053] Wheat ears at the waxy maturity stage were harvested and air-dried indoors. After 7 days, they were threshed, and the moisture content was measured to be 10%-12%. After sterilization and rinsing with 0.5% NaClO, they were placed in petri dishes (with moistened filter paper at the bottom). Starting on the second day of the experiment, the number of germinated seeds was counted, and germinated seeds were removed. This counting was continued for 7 days. The germination rate was calculated as: Germination rate = (Number of germinated seeds / Total number of seeds) × 100%. Results are as follows... Figure 6 As shown, WT's 7-day germination rate exceeds 80%, while TaMyb10-94E Overexpression significantly inhibits grain germination rate.

[0054] After harvesting mature seeds, they were treated with vanillin and NaOH solution, respectively. NaOH reacts with flavonols and dihydroflavones, turning brown or yellow. The results are as follows... Figure 7 As shown. TaMyb10-94E Overexpression (2# and 12#) causes wheat grains to turn white, while other wheat grains... TaMyb10 Gene overexpression (58-4#) causes wheat grains to turn red.

[0055] In summary, natural wheat materials contain substances that do not include... TaMyb10 Studies have shown that the gene is overexpressed in common wheat, such as Fielder (white-grained, not resistant to ear sprouting), in the Fielder background. TaMyb10-94E The genetically modified wheat has white grains and resistance to ear bud break ( Figure 6 and Figure 7 In the Fielder context, overexpression of other wheat species... TaMyb10The genetically modified wheat has red grains and resistance to ear bud break, indicating that... TaMyb10-94E The gene has the function of producing resistance to ear bud break without producing red grains. TaMyb10-94E Regulates wheat grain color and germination.

[0056] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. Germination resistance gene in white-grained wheat ears TaMyb10-94E Its characteristics are, It is a gene that encodes a protein with an amino acid sequence as shown in SEQ ID NO:

1.

2. The gene according to claim 1 TaMyb10-94E Its characteristics are, Its nucleotide sequence is as follows: i) The nucleotide sequence shown in SEQ ID NO:2; ii) A nucleotide sequence of the nucleotide sequence shown in SEQ ID NO:2 that has been substituted, deleted and / or added with one or more nucleotides and expresses a protein with the same function; iii) A nucleotide sequence that hybridizes with the sequence shown in SEQ ID NO:2 under stringent conditions and expresses a protein with the same function, wherein the stringent conditions are hybridization at 65°C in 0.1×SSPE containing 0.1% SDS or 0.1×SSC containing 0.1% SDS, followed by washing the membrane with the same solution; or iv) Nucleotide sequences that have more than 90% homology with the nucleotide sequences of i), ii) or iii) and express the same functional protein.

3. Containing the gene described in claim 1 or 2 TaMyb10-94E The biomaterial is characterized by, The biological material is recombinant DNA, expression cassette, transposon, plasmid vector, viral vector, or engineered bacteria.

4. The gene according to claim 1 or 2 TaMyb10-94E Or any of the following applications of the biomaterial described in claim 3: (1) Used to obtain white-grained wheat resistant to ear sprouting; (2) Used to reduce the germination rate of wheat grains; (3) Used for plant breeding or plant germplasm resource improvement. The purpose of breeding or germplasm resource improvement is to obtain white-grained wheat resistant to ear sprouting and reduce the germination rate of wheat grains. (4) Used to prepare genetically modified wheat. The purpose of genetic modification is to obtain white-grained wheat resistant to ear germination and reduce the germination rate of wheat grains.

5. The application according to claim 4, characterized in that, The applications include: 1) Make wheat contain genes TaMyb10-94E ;or 2) Enable wheat to express genes TaMyb10-94E Encoded protein; or 3) Cause wheat to overexpress genes TaMyb10-94E ; This allows for the production of white-grained wheat resistant to ear sprouting.

6. A method for constructing white-grained wheat resistant to ear sprouting, characterized in that, The method includes: using genetic engineering techniques to overexpress the gene of claim 1 or 2 in wheat. TaMyb10-94E ; The overexpression method is selected from the following 1) to 6), or any combination thereof: 1) By importing a plasmid containing the gene; 2) By increasing the copy number of the aforementioned genes on the wheat chromosome; 3) By altering the promoter sequences of the aforementioned genes on the wheat chromosome; 4) By operatively linking a strong promoter to the gene; 5) By introducing enhancers; 6) Using gene editing techniques to remove certain components from wheat. TaMyb10 Genetically modified TaMyb10-94E Gene.

7. The method according to claim 6, characterized in that, The expression vector containing the gene was transformed into Agrobacterium, and the gene was transferred into wheat through Agrobacterium-mediated transformation, thereby overexpressing the gene in wheat.

8. The application of the transgenic wheat obtained according to the method of claim 6 or 7 in plant breeding; The purpose of breeding is to obtain white-grained wheat resistant to ear germination and reduce the germination rate of wheat grains.

9. The application according to claim 8, characterized in that, Breeding methods include transgenic, hybridization, backcrossing, self-pollination, or asexual reproduction.

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