Polycyclic aromatic hydrocarbon degradation bacterial membrane vesicle as well as preparation method and application thereof

Abstract

The invention discloses a polycyclic aromatic hydrocarbon degrading bacterial membrane vesicle and a preparation method and application thereof, and relates to the field of microbiology, the polycyclic aromatic hydrocarbon degrading bacterial membrane vesicle (MVs) is separated and purified from polycyclic aromatic hydrocarbon degrading bacteria and has the function of assisting the bacteria to cope with the oxidation pressure of benzo [a] pyrene, and the polycyclic aromatic hydrocarbon degrading bacteria are novosphingobacterium pentaromativorans US6-1; the cultured bacterial liquid of the polycyclic aromatic hydrocarbon degrading bacteria is subjected to high-speed centrifugation, precipitates are discarded, supernate is collected, then filtering and centrifugal concentration are performed to obtain supernate concentrated liquid, ultracentrifugation is performed, and precipitates are the membrane vesicles. The MVs secreted by the polycyclic aromatic hydrocarbon degrading bacteria are separated and purified for the first time, the prepared MVs can be used as a delivery carrier to deliver biomolecules with an antioxidant function to recipient bacteria, and the MVs have a good effect of assisting the degrading bacteria to resist oxidation pressure generated in the process of degrading benzo [a] pyrene.

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