5'UTR元件、表达载体及其应用

By providing a 5'UTR element with a specific nucleotide sequence, the problem of low expression efficiency and capping efficiency in in vitro synthesized mRNA was solved, achieving efficient mRNA expression and capping effects.

CN119842704BActive Publication Date: 2026-07-17YUNZHOU BIOSCIENCES (GUANGZHOU) INC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YUNZHOU BIOSCIENCES (GUANGZHOU) INC
Filing Date
2024-05-06
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

The 5'UTR sequence of in vitro synthesized mRNA cannot simultaneously achieve high expression efficiency and high capping efficiency, affecting the stability and expression efficiency of mRNA.

Method used

Provides 5'UTR elements with specific nucleotide sequences, including sequences shown in SEQ ID NO:9 to SEQ ID NO:62 and their variants, for constructing expression vectors to improve mRNA capping and expression efficiency.

Benefits of technology

It achieved a capping efficiency of over 80% for mRNA and significantly improved expression efficiency, which is superior to existing modified 5'UTR sequences.

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Abstract

本发明涉及体外转录领域，尤其涉及5'UTR元件、表达载体及其应用。本发明提供了5'UTR元件，所述5'UTR元件具有：如SEQ ID NO:9至SEQ ID NO:12任意所示的核苷酸序列；或如所示的核苷酸序列经取代、缺失或添加一个或多个碱基获得的核苷酸序列，且与所示的核苷酸序列功能相同或相似的核苷酸序列；或与所示的核苷酸序列至少有80％同一性的核苷酸序列。本发明通过提供5'UTR序列，解决体外合成mRNA时，原核生物5'UTR序列无法同时具有既能使mRNA表达效率高，又能使其加帽效率高的功能的问题。
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